Restriction map for
pF1KSDA0859
(Linear) (Six-base) MAPSORT of: KIAA0859.seq Check: 299 from: 1 to: 2097
KIAA0859.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 441 2097
Size: 441 1656
AccI GT'mk_AC
Cuts at: 0 1236 2097
Size: 1236 861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 643 972 1309 1841 2097
Size: 520 123 329 337 532 256
Fragments arranged by size:
532 520 337 329 256 123
AflIII A'CryG_T
Cuts at: 0 2054 2097
Size: 2054 43
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 715 790 1364 1716 1994 2097
Size: 715 75 574 352 278 103
Fragments arranged by size:
715 574 352 278 103 75
AlwNI CAG_nnn'CTG
Cuts at: 0 646 2097
Size: 646 1451
ApaLI G'TGCA_C
Cuts at: 0 547 2097
Size: 547 1550
AvaI C'yCGr_G
Cuts at: 0 524 1377 2097
Size: 524 853 720
Fragments arranged by size:
853 720 524
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1218 1251 2097
Size: 1218 33 846
Fragments arranged by size:
1218 846 33
BanI G'GyrC_C
Cuts at: 0 364 2097
Size: 364 1733
BanII G_rGCy'C
Cuts at: 0 1889 1994 2097
Size: 1889 105 103
Fragments arranged by size:
1889 105 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 406 2097
Size: 406 1691
BbvCI CC'TCA_GC
Cuts at: 0 431 1249 2097
Size: 431 818 848
Fragments arranged by size:
848 818 431
BglI GCCn_nnn'nGGC
Cuts at: 0 702 1675 2097
Size: 702 973 422
Fragments arranged by size:
973 702 422
BglII A'GATC_T
Cuts at: 0 662 2097
Size: 662 1435
BlpI GC'TnA_GC
Cuts at: 0 1055 2097
Size: 1055 1042
Bme1580I G_kGCm'C
Cuts at: 0 367 551 2097
Size: 367 184 1546
Fragments arranged by size:
1546 367 184
BmrI ACTGGGnnnn_n'
Cuts at: 0 1596 1756 2097
Size: 1596 160 341
Fragments arranged by size:
1596 341 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 51 821 2097
Size: 51 770 1276
Fragments arranged by size:
1276 770 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 2097
Size: 727 1370
Bpu10I CC'TnA_GC
Cuts at: 0 431 485 680 1249 1967 2097
Size: 431 54 195 569 718 130
Fragments arranged by size:
718 569 431 195 130 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1200 1873 2062 2097
Size: 1200 673 189 35
Fragments arranged by size:
1200 673 189 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 922 2097
Size: 922 1175
BsaAI yAC'GTr
Cuts at: 0 855 2057 2097
Size: 855 1202 40
Fragments arranged by size:
1202 855 40
BsaBI GATnn'nnATC
Cuts at: 0 907 2097
Size: 907 1190
BsaWI w'CCGG_w
Cuts at: 0 1148 2097
Size: 1148 949
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 919 949 1976 2006 2097
Size: 919 30 1027 30 91
Fragments arranged by size:
1027 919 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1697 1700 2097
Size: 1697 3 397
Fragments arranged by size:
1697 397 3
BseYI C'CCAG_C
Cuts at: 0 792 1086 1090 1103 1767 1882 2097
Size: 792 294 4 13 664 115 215
Fragments arranged by size:
792 664 294 215 115 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 786 1288 1945 2097
Size: 757 29 502 657 152
Fragments arranged by size:
757 657 502 152 29
BsiEI CG_ry'CG
Cuts at: 0 462 2097
Size: 462 1635
BsiHKAI G_wGCw'C
Cuts at: 0 551 681 1994 2097
Size: 551 130 1313 103
Fragments arranged by size:
1313 551 130 103
Bsp1286I G_dGCh'C
Cuts at: 0 367 551 681 1889 1994 2097
Size: 367 184 130 1208 105 103
Fragments arranged by size:
1208 367 184 130 105 103
BspEI T'CCGG_A
Cuts at: 0 1148 2097
Size: 1148 949
BspHI T'CATG_A
Cuts at: 0 623 2097
Size: 623 1474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 441 1101 2097
Size: 441 660 996
Fragments arranged by size:
996 660 441
BsrBI CCG'CTC
Cuts at: 0 720 1184 2019 2097
Size: 720 464 835 78
Fragments arranged by size:
835 720 464 78
BsrDI GCAATG_nn'
Cuts at: 0 1643 2097
Size: 1643 454
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1609 2097
Size: 1609 488
BstYI r'GATC_y
Cuts at: 0 662 1475 1943 2097
Size: 662 813 468 154
Fragments arranged by size:
813 662 468 154
Bsu36I CC'TnA_GG
Cuts at: 0 917 2026 2097
Size: 917 1109 71
Fragments arranged by size:
1109 917 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 833 866 2097
Size: 833 33 1231
Fragments arranged by size:
1231 833 33
BtsI GCAGTG_nn'
Cuts at: 0 515 1901 2097
Size: 515 1386 196
Fragments arranged by size:
1386 515 196
ClaI AT'CG_AT
Cuts at: 0 1574 2097
Size: 1574 523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1469 1504 2097
Size: 1469 35 593
Fragments arranged by size:
1469 593 35
DraIII CAC_nnn'GTG
Cuts at: 0 555 858 1604 2097
Size: 555 303 746 493
Fragments arranged by size:
746 555 493 303
DrdI GACnn_nn'nnGTC
Cuts at: 0 1144 2059 2097
Size: 1144 915 38
Fragments arranged by size:
1144 915 38
EaeI y'GGCC_r
Cuts at: 0 438 557 725 974 1595 2097
Size: 438 119 168 249 621 502
Fragments arranged by size:
621 502 438 249 168 119
EarI CTCTTCn'nnn_
Cuts at: 0 389 2097
Size: 389 1708
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1751 2097
Size: 1751 346
EcoICRI GAG'CTC
Cuts at: 0 1992 2097
Size: 1992 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 643 727 972 1309 1841 2097
Size: 520 123 84 245 337 532 256
Fragments arranged by size:
532 520 337 256 245 123 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 484 2097
Size: 484 1613
EcoO109I rG'GnC_Cy
Cuts at: 0 1113 1362 1737 2097
Size: 1113 249 375 360
Fragments arranged by size:
1113 375 360 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1245 2097
Size: 1245 852
HincII GTy'rAC
Cuts at: 0 42 1237 1728 2097
Size: 42 1195 491 369
Fragments arranged by size:
1195 491 369 42
HindIII A'AGCT_T
Cuts at: 0 80 2097
Size: 80 2017
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 560 1568 2097
Size: 335 225 1008 529
Fragments arranged by size:
1008 529 335 225
MscI TGG'CCA
Cuts at: 0 559 1597 2097
Size: 559 1038 500
Fragments arranged by size:
1038 559 500
MspA1I CmG'CkG
Cuts at: 0 774 969 1090 1344 1959 2097
Size: 774 195 121 254 615 138
Fragments arranged by size:
774 615 254 195 138 121
PasI CC'CwG_GG
Cuts at: 0 143 2097
Size: 143 1954
PflMI CCAn_nnn'nTGG
Cuts at: 0 579 646 858 1604 2013 2097
Size: 579 67 212 746 409 84
Fragments arranged by size:
746 579 409 212 84 67
PmlI CAC'GTG
Cuts at: 0 855 2097
Size: 855 1242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1933 1965 2097
Size: 580 32 1321 32 132
Fragments arranged by size:
1321 580 132 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1113 1362 1737 2097
Size: 1113 249 375 360
Fragments arranged by size:
1113 375 360 249
PstI C_TGCA'G
Cuts at: 0 452 1174 1374 2097
Size: 452 722 200 723
Fragments arranged by size:
723 722 452 200
PvuII CAG'CTG
Cuts at: 0 774 969 1090 1959 2097
Size: 774 195 121 869 138
Fragments arranged by size:
869 774 195 138 121
RsrII CG'GwC_CG
Cuts at: 0 1151 2097
Size: 1151 946
SacI G_AGCT'C
Cuts at: 0 1994 2097
Size: 1994 103
SalI G'TCGA_C
Cuts at: 0 1235 2097
Size: 1235 862
SbfI CC_TGCA'GG
Cuts at: 0 452 2097
Size: 452 1645
SexAI A'CCwGG_T
Cuts at: 0 572 2097
Size: 572 1525
SfcI C'TryA_G
Cuts at: 0 448 1170 1370 1792 2097
Size: 448 722 200 422 305
Fragments arranged by size:
722 448 422 305 200
SmaI CCC'GGG
Cuts at: 0 526 1379 2097
Size: 526 853 718
Fragments arranged by size:
853 718 526
SmlI C'TyrA_G
Cuts at: 0 1179 1888 2077 2097
Size: 1179 709 189 20
Fragments arranged by size:
1179 709 189 20
StuI AGG'CCT
Cuts at: 0 1502 2097
Size: 1502 595
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 155 946 1117 1120 1615 1643 2097
Size: 155 791 171 3 495 28 454
Fragments arranged by size:
791 495 454 171 155 28 3
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 919 951 1976 2008 2097
Size: 919 32 1025 32 89
Fragments arranged by size:
1025 919 89 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1192 2097
Size: 1192 905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1590 1648 2097
Size: 1590 58 449
Fragments arranged by size:
1590 449 58
XmaI C'CCGG_G
Cuts at: 0 524 1377 2097
Size: 524 853 720
Fragments arranged by size:
853 720 524
XmnI GAAnn'nnTTC
Cuts at: 0 58 82 2097
Size: 58 24 2015
Fragments arranged by size:
2015 58 24
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaLI AvaI
BaeI BanI BanII BbsI BbvCI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstXI BstYI
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HincII
HindIII MmeI MscI MspA1I PasI PflMI PmlI PpiI
PpuMI PstI PvuII RsrII SacI SalI SbfI SexAI
SfcI SmaI SmlI StuI TaqII TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BsaHI
BsiWI BsmI BsmBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI DraI EagI EcoRI EcoRV
FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PshAI PsiI PspOMI PspXI PsrI PvuI SacII
SanDI SapI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI XbaI
XhoI ZraI