Restriction map for
pF1KSDA0857
(Linear) (Six-base) MAPSORT of: KIAA0857.seq Check: 9024 from: 1 to: 1959
KIAA0857.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 359 1959
Size: 359 1600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 881 955 1774 1959
Size: 552 329 74 819 185
Fragments arranged by size:
819 552 329 185 74
AflIII A'CryG_T
Cuts at: 0 128 1959
Size: 128 1831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 1959
Size: 733 1226
AlwNI CAG_nnn'CTG
Cuts at: 0 1875 1959
Size: 1875 84
ApaI G_GGCC'C
Cuts at: 0 84 243 283 960 1051 1278 1951 1959
Size: 84 159 40 677 91 227 673 8
Fragments arranged by size:
677 673 227 159 91 84 40 8
AvaI C'yCGr_G
Cuts at: 0 105 199 953 1074 1944 1959
Size: 105 94 754 121 870 15
Fragments arranged by size:
870 754 121 105 94 15
BamHI G'GATC_C
Cuts at: 0 654 1959
Size: 654 1305
BanI G'GyrC_C
Cuts at: 0 285 811 1058 1959
Size: 285 526 247 901
Fragments arranged by size:
901 526 285 247
BanII G_rGCy'C
Cuts at: 0 84 106 243 283 304 760 960 1043
Size: 84 22 137 40 21 456 200 83
Cuts at: 1043 1051 1081 1278 1359 1814 1951 1959
Size: 8 30 197 81 455 137 8
Fragments arranged by size:
456 455 200 197 137 137 84 83
81 40 30 22 21 8 8
BbeI G_GCGC'C
Cuts at: 0 289 815 1959
Size: 289 526 1144
Fragments arranged by size:
1144 526 289
BbsI GAAGACnn'nnnn_
Cuts at: 0 190 393 1959
Size: 190 203 1566
Fragments arranged by size:
1566 203 190
BbvCI CC'TCA_GC
Cuts at: 0 777 1959
Size: 777 1182
BglI GCCn_nnn'nGGC
Cuts at: 0 385 1246 1723 1959
Size: 385 861 477 236
Fragments arranged by size:
861 477 385 236
BlpI GC'TnA_GC
Cuts at: 0 1846 1959
Size: 1846 113
Bme1580I G_kGCm'C
Cuts at: 0 84 243 283 960 1051 1278 1951 1959
Size: 84 159 40 677 91 227 673 8
Fragments arranged by size:
677 673 227 159 91 84 40 8
BmgBI CAC'GTC
Cuts at: 0 60 1959
Size: 60 1899
BmrI ACTGGGnnnn_n'
Cuts at: 0 732 1559 1959
Size: 732 827 400
Fragments arranged by size:
827 732 400
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 234 1959
Size: 234 1725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1280 1620 1687 1897 1959
Size: 1280 340 67 210 62
Fragments arranged by size:
1280 340 210 67 62
Bpu10I CC'TnA_GC
Cuts at: 0 517 777 1223 1654 1959
Size: 517 260 446 431 305
Fragments arranged by size:
517 446 431 305 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1554 1959
Size: 1554 405
BsaI GGTCTCn'nnnn_
Cuts at: 0 566 1393 1909 1959
Size: 566 827 516 50
Fragments arranged by size:
827 566 516 50
BsaHI Gr'CG_yC
Cuts at: 0 286 356 812 1959
Size: 286 70 456 1147
Fragments arranged by size:
1147 456 286 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1051 1081 1959
Size: 1051 30 878
Fragments arranged by size:
1051 878 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 811 938 941 1051 1144 1225 1279
Size: 226 585 127 3 110 93 81 54
Cuts at: 1279 1282 1317 1959
Size: 3 35 642
Fragments arranged by size:
642 585 226 127 110 93 81 54
35 3 3
BseYI C'CCAG_C
Cuts at: 0 419 753 1959
Size: 419 334 1206
Fragments arranged by size:
1206 419 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 863 1888 1959
Size: 427 436 1025 71
Fragments arranged by size:
1025 436 427 71
BsiEI CG_ry'CG
Cuts at: 0 1893 1959
Size: 1893 66
BsiHKAI G_wGCw'C
Cuts at: 0 106 220 304 329 1224 1814 1959
Size: 106 114 84 25 895 590 145
Fragments arranged by size:
895 590 145 114 106 84 25
BsmI GAATG_Cn'
Cuts at: 0 1697 1959
Size: 1697 262
Bsp1286I G_dGCh'C
Cuts at: 0 84 106 220 243 283 304 329 760
Size: 84 22 114 23 40 21 25 431
Cuts at: 760 960 1043 1051 1081 1224 1278 1359 1814
Size: 200 83 8 30 143 54 81 455
Cuts at: 1814 1951 1959
Size: 137 8
Fragments arranged by size:
455 431 200 143 137 114 84 83
81 54 40 30 25 23 22 21
8 8
BsrBI CCG'CTC
Cuts at: 0 112 344 830 998 1634 1839 1959
Size: 112 232 486 168 636 205 120
Fragments arranged by size:
636 486 232 205 168 120 112
BsrFI r'CCGG_y
Cuts at: 0 24 1892 1959
Size: 24 1868 67
Fragments arranged by size:
1868 67 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 808 1959
Size: 808 1151
BstYI r'GATC_y
Cuts at: 0 654 1937 1959
Size: 654 1283 22
Fragments arranged by size:
1283 654 22
BtgI C'CryG_G
Cuts at: 0 84 380 1959
Size: 84 296 1579
Fragments arranged by size:
1579 296 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 935 1959
Size: 935 1024
BtsI GCAGTG_nn'
Cuts at: 0 436 1202 1665 1959
Size: 436 766 463 294
Fragments arranged by size:
766 463 436 294
DraIII CAC_nnn'GTG
Cuts at: 0 431 1578 1959
Size: 431 1147 381
Fragments arranged by size:
1147 431 381
EaeI y'GGCC_r
Cuts at: 0 1728 1781 1959
Size: 1728 53 178
Fragments arranged by size:
1728 178 53
EarI CTCTTCn'nnn_
Cuts at: 0 1005 1119 1396 1959
Size: 1005 114 277 563
Fragments arranged by size:
1005 563 277 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 294 1959
Size: 294 1665
EcoICRI GAG'CTC
Cuts at: 0 104 302 1812 1959
Size: 104 198 1510 147
Fragments arranged by size:
1510 198 147 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 881 955 1280 1620 1687 1774 1897
Size: 552 329 74 325 340 67 87 123
Cuts at: 1897 1959
Size: 62
Fragments arranged by size:
552 340 329 325 123 87 74 67
62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 1959
Size: 373 1586
EcoO109I rG'GnC_Cy
Cuts at: 0 34 239 240 805 911 957 1047 1048
Size: 34 205 1 565 106 46 90 1
Cuts at: 1048 1135 1274 1462 1475 1548 1716 1948 1959
Size: 87 139 188 13 73 168 232 11
Fragments arranged by size:
565 232 205 188 168 139 106 90
87 73 46 34 13 11 1 1
FspI TGC'GCA
Cuts at: 0 721 1959
Size: 721 1238
HaeII r_GCGC'y
Cuts at: 0 289 391 524 647 815 1959
Size: 289 102 133 123 168 1144
Fragments arranged by size:
1144 289 168 133 123 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 976 1008 1959
Size: 601 32 343 32 951
Fragments arranged by size:
951 601 343 32 32
HincII GTy'rAC
Cuts at: 0 360 1959
Size: 360 1599
KasI G'GCGC_C
Cuts at: 0 285 811 1959
Size: 285 526 1148
Fragments arranged by size:
1148 526 285
MluI A'CGCG_T
Cuts at: 0 128 1959
Size: 128 1831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1399 1959
Size: 767 632 560
Fragments arranged by size:
767 632 560
MscI TGG'CCA
Cuts at: 0 1730 1959
Size: 1730 229
MslI CAynn'nnrTG
Cuts at: 0 334 983 1959
Size: 334 649 976
Fragments arranged by size:
976 649 334
MspA1I CmG'CkG
Cuts at: 0 44 86 785 848 1765 1833 1854 1959
Size: 44 42 699 63 917 68 21 105
Fragments arranged by size:
917 699 105 68 63 44 42 21
NaeI GCC'GGC
Cuts at: 0 26 1959
Size: 26 1933
NarI GG'CG_CC
Cuts at: 0 286 812 1959
Size: 286 526 1147
Fragments arranged by size:
1147 526 286
NgoMIV G'CCGG_C
Cuts at: 0 24 1959
Size: 24 1935
NspI r_CATG'y
Cuts at: 0 1867 1959
Size: 1867 92
PasI CC'CwG_GG
Cuts at: 0 290 1044 1243 1959
Size: 290 754 199 716
Fragments arranged by size:
754 716 290 199
PflMI CCAn_nnn'nTGG
Cuts at: 0 1093 1737 1959
Size: 1093 644 222
Fragments arranged by size:
1093 644 222
PpuMI rG'GwC_Cy
Cuts at: 0 805 911 1462 1548 1959
Size: 805 106 551 86 411
Fragments arranged by size:
805 551 411 106 86
PspOMI G'GGCC_C
Cuts at: 0 80 239 279 956 1047 1274 1947 1959
Size: 80 159 40 677 91 227 673 12
Fragments arranged by size:
677 673 227 159 91 80 40 12
PstI C_TGCA'G
Cuts at: 0 1832 1937 1959
Size: 1832 105 22
Fragments arranged by size:
1832 105 22
PvuII CAG'CTG
Cuts at: 0 848 1765 1959
Size: 848 917 194
Fragments arranged by size:
917 848 194
SacI G_AGCT'C
Cuts at: 0 106 304 1814 1959
Size: 106 198 1510 145
Fragments arranged by size:
1510 198 145 106
SacII CC_GC'GG
Cuts at: 0 87 1959
Size: 87 1872
SalI G'TCGA_C
Cuts at: 0 358 1959
Size: 358 1601
SanDI GG'GwC_CC
Cuts at: 0 1462 1959
Size: 1462 497
SapI GCTCTTCn'nnn_
Cuts at: 0 1005 1959
Size: 1005 954
ScaI AGT'ACT
Cuts at: 0 1789 1959
Size: 1789 170
SfcI C'TryA_G
Cuts at: 0 915 1828 1933 1959
Size: 915 913 105 26
Fragments arranged by size:
915 913 105 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1246 1959
Size: 1246 713
SfoI GGC'GCC
Cuts at: 0 287 813 1959
Size: 287 526 1146
Fragments arranged by size:
1146 526 287
SmaI CCC'GGG
Cuts at: 0 1946 1959
Size: 1946 13
SmlI C'TyrA_G
Cuts at: 0 1533 1959
Size: 1533 426
StuI AGG'CCT
Cuts at: 0 1412 1959
Size: 1412 547
StyI C'CwwG_G
Cuts at: 0 556 1407 1544 1959
Size: 556 851 137 415
Fragments arranged by size:
851 556 415 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 916 1329 1938 1959
Size: 916 413 609 21
Fragments arranged by size:
916 609 413 21
TatI w'GTAC_w
Cuts at: 0 161 1787 1959
Size: 161 1626 172
Fragments arranged by size:
1626 172 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1959
Size: 1051 32 876
Fragments arranged by size:
1051 876 32
Tth111I GACn'n_nGTC
Cuts at: 0 397 738 1959
Size: 397 341 1221
Fragments arranged by size:
1221 397 341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 387 750 789 1317 1959
Size: 387 363 39 528 642
Fragments arranged by size:
642 528 387 363 39
XmaI C'CCGG_G
Cuts at: 0 1944 1959
Size: 1944 15
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BsrBI BsrFI BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PflMI PpuMI
PspOMI PstI PvuII SacI SacII SalI SanDI SapI
ScaI SfcI SfiI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglII BmtI BsaAI
BsaBI BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SbfI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI XmnI ZraI