Restriction map for
pF1KSDA0846
(Linear) (Six-base) MAPSORT of: KIAA0846.seq Check: 9057 from: 1 to: 2067
KIAA0846.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AccI GT'mk_AC
Cuts at: 0 1997 2067
Size: 1997 70
Acc65I G'GTAC_C
Cuts at: 0 1194 2067
Size: 1194 873
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 682 776 1127 2067
Size: 268 414 94 351 940
Fragments arranged by size:
940 414 351 268 94
AflIII A'CryG_T
Cuts at: 0 339 2067
Size: 339 1728
AhdI GACnn_n'nnGTC
Cuts at: 0 1881 2067
Size: 1881 186
AlwNI CAG_nnn'CTG
Cuts at: 0 1864 2067
Size: 1864 203
ApaLI G'TGCA_C
Cuts at: 0 48 2067
Size: 48 2019
ApoI r'AATT_y
Cuts at: 0 205 301 708 1149 2067
Size: 205 96 407 441 918
Fragments arranged by size:
918 441 407 205 96
AvaI C'yCGr_G
Cuts at: 0 1640 1878 2067
Size: 1640 238 189
Fragments arranged by size:
1640 238 189
BanI G'GyrC_C
Cuts at: 0 1194 1644 2067
Size: 1194 450 423
Fragments arranged by size:
1194 450 423
BanII G_rGCy'C
Cuts at: 0 458 2067
Size: 458 1609
BbeI G_GCGC'C
Cuts at: 0 1648 2067
Size: 1648 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1510 1544 2067
Size: 1510 34 523
Fragments arranged by size:
1510 523 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 423 1555 2067
Size: 423 1132 512
Fragments arranged by size:
1132 512 423
BclI T'GATC_A
Cuts at: 0 1067 1293 2067
Size: 1067 226 774
Fragments arranged by size:
1067 774 226
BglII A'GATC_T
Cuts at: 0 218 1796 2067
Size: 218 1578 271
Fragments arranged by size:
1578 271 218
BlpI GC'TnA_GC
Cuts at: 0 470 2067
Size: 470 1597
Bme1580I G_kGCm'C
Cuts at: 0 52 2008 2067
Size: 52 1956 59
Fragments arranged by size:
1956 59 52
BmgBI CAC'GTC
Cuts at: 0 342 2067
Size: 342 1725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 849 2067
Size: 849 1218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 472 508 574 1159 1222 1748 2067
Size: 209 263 36 66 585 63 526 319
Fragments arranged by size:
585 526 319 263 209 66 63 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1489 1674 2067
Size: 1489 185 393
Fragments arranged by size:
1489 393 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 766 837 2035 2067
Size: 766 71 1198 32
Fragments arranged by size:
1198 766 71 32
BsaHI Gr'CG_yC
Cuts at: 0 1177 1645 2067
Size: 1177 468 422
Fragments arranged by size:
1177 468 422
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 982 1012 2067
Size: 982 30 1055
Fragments arranged by size:
1055 982 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 825 976 2067
Size: 825 151 1091
Fragments arranged by size:
1091 825 151
BseYI C'CCAG_C
Cuts at: 0 644 1698 1868 2067
Size: 644 1054 170 199
Fragments arranged by size:
1054 644 199 170
BsiHKAI G_wGCw'C
Cuts at: 0 52 476 2067
Size: 52 424 1591
Fragments arranged by size:
1591 424 52
BsmI GAATG_Cn'
Cuts at: 0 365 1038 2067
Size: 365 673 1029
Fragments arranged by size:
1029 673 365
Bsp1286I G_dGCh'C
Cuts at: 0 52 458 476 2008 2067
Size: 52 406 18 1532 59
Fragments arranged by size:
1532 406 59 52 18
BspHI T'CATG_A
Cuts at: 0 230 2067
Size: 230 1837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1082 2067
Size: 1082 985
BsrDI GCAATG_nn'
Cuts at: 0 207 2067
Size: 207 1860
BstAPI GCAn_nnn'nTGC
Cuts at: 0 851 2067
Size: 851 1216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 465 1773 2067
Size: 150 315 1308 294
Fragments arranged by size:
1308 315 294 150
BstYI r'GATC_y
Cuts at: 0 218 273 509 1796 2067
Size: 218 55 236 1287 271
Fragments arranged by size:
1287 271 236 218 55
BtgI C'CryG_G
Cuts at: 0 543 1238 2067
Size: 543 695 829
Fragments arranged by size:
829 695 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 896 2067
Size: 896 1171
BtsI GCAGTG_nn'
Cuts at: 0 734 1665 1672 1733 2067
Size: 734 931 7 61 334
Fragments arranged by size:
931 734 334 61 7
ClaI AT'CG_AT
Cuts at: 0 580 2067
Size: 580 1487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1560 1595 2067
Size: 1560 35 472
Fragments arranged by size:
1560 472 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1240 2067
Size: 1240 827
EarI CTCTTCn'nnn_
Cuts at: 0 384 1791 2033 2067
Size: 384 1407 242 34
Fragments arranged by size:
1407 384 242 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 268 472 508 574 682 776 1127
Size: 209 59 204 36 66 108 94 351
Cuts at: 1127 1159 1222 1748 2067
Size: 32 63 526 319
Fragments arranged by size:
526 351 319 209 204 108 94 66
63 59 36 32
EcoO109I rG'GnC_Cy
Cuts at: 0 1883 2067
Size: 1883 184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1530 2067
Size: 1530 537
HaeII r_GCGC'y
Cuts at: 0 1648 2067
Size: 1648 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 203 235 481 513 765 797 1485 1517
Size: 203 32 246 32 252 32 688 32
Cuts at: 1517 1647 1679 1867 1899 2067
Size: 130 32 188 32 168
Fragments arranged by size:
688 252 246 203 188 168 130 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 322 2067
Size: 322 1745
KasI G'GCGC_C
Cuts at: 0 1644 2067
Size: 1644 423
KpnI G_GTAC'C
Cuts at: 0 1198 2067
Size: 1198 869
MfeI C'AATT_G
Cuts at: 0 1587 2067
Size: 1587 480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 863 2067
Size: 589 274 1204
Fragments arranged by size:
1204 589 274
MslI CAynn'nnrTG
Cuts at: 0 61 721 1301 1982 2067
Size: 61 660 580 681 85
Fragments arranged by size:
681 660 580 85 61
NarI GG'CG_CC
Cuts at: 0 1645 2067
Size: 1645 422
NcoI C'CATG_G
Cuts at: 0 543 2067
Size: 543 1524
NspI r_CATG'y
Cuts at: 0 174 1981 2067
Size: 174 1807 86
Fragments arranged by size:
1807 174 86
PasI CC'CwG_GG
Cuts at: 0 1970 2067
Size: 1970 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 2067
Size: 621 1446
PpuMI rG'GwC_Cy
Cuts at: 0 1883 2067
Size: 1883 184
PstI C_TGCA'G
Cuts at: 0 261 1031 1630 2067
Size: 261 770 599 437
Fragments arranged by size:
770 599 437 261
SanDI GG'GwC_CC
Cuts at: 0 1883 2067
Size: 1883 184
SapI GCTCTTCn'nnn_
Cuts at: 0 1791 2067
Size: 1791 276
SbfI CC_TGCA'GG
Cuts at: 0 1630 2067
Size: 1630 437
SfcI C'TryA_G
Cuts at: 0 257 1027 1626 1810 2067
Size: 257 770 599 184 257
Fragments arranged by size:
770 599 257 257 184
SfoI GGC'GCC
Cuts at: 0 1646 2067
Size: 1646 421
SmaI CCC'GGG
Cuts at: 0 1642 2067
Size: 1642 425
SmlI C'TyrA_G
Cuts at: 0 1504 1653 2067
Size: 1504 149 414
Fragments arranged by size:
1504 414 149
SpeI A'CTAG_T
Cuts at: 0 1017 2067
Size: 1017 1050
SphI G_CATG'C
Cuts at: 0 1981 2067
Size: 1981 86
SrfI GCCC'GGGC
Cuts at: 0 1642 2067
Size: 1642 425
StuI AGG'CCT
Cuts at: 0 740 868 2067
Size: 740 128 1199
Fragments arranged by size:
1199 740 128
StyI C'CwwG_G
Cuts at: 0 15 543 569 903 2067
Size: 15 528 26 334 1164
Fragments arranged by size:
1164 528 334 26 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1852 2067
Size: 1852 215
TatI w'GTAC_w
Cuts at: 0 909 2067
Size: 909 1158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014 2067
Size: 982 32 1053
Fragments arranged by size:
1053 982 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1188 2067
Size: 1188 879
XmaI C'CCGG_G
Cuts at: 0 1640 2067
Size: 1640 427
XmnI GAAnn'nnTTC
Cuts at: 0 161 2067
Size: 161 1906
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DrdI EarI Eco57MI EcoO109I FalI HaeII Hin4I
HincII KasI KpnI MfeI MmeI MslI NarI NcoI
NspI PasI PflMI PpuMI PstI SanDI SapI SbfI
SfcI SfoI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BfrBI BglI BmrI BmtI Bpu10I BsaAI
BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI MluI MscI
MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI ScaI SexAI SfiI SgfI SgrAI SnaBI SspI
SwaI Tth111I XbaI XhoI ZraI