Restriction map for
pF1KSDA0844
(Linear) (Six-base) MAPSORT of: KIAA0844.seq Check: 376 from: 1 to: 1221
KIAA0844.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AflIII A'CryG_T
Cuts at: 0 477 1221
Size: 477 744
ApaI G_GGCC'C
Cuts at: 0 135 1221
Size: 135 1086
ApoI r'AATT_y
Cuts at: 0 490 1221
Size: 490 731
AvaI C'yCGr_G
Cuts at: 0 84 1221
Size: 84 1137
BanI G'GyrC_C
Cuts at: 0 971 1221
Size: 971 250
BanII G_rGCy'C
Cuts at: 0 135 1145 1221
Size: 135 1010 76
Fragments arranged by size:
1010 135 76
BglI GCCn_nnn'nGGC
Cuts at: 0 1163 1221
Size: 1163 58
Bme1580I G_kGCm'C
Cuts at: 0 135 920 974 1221
Size: 135 785 54 247
Fragments arranged by size:
785 247 135 54
BmgBI CAC'GTC
Cuts at: 0 669 1221
Size: 669 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 796 1221
Size: 160 636 425
Fragments arranged by size:
636 425 160
Bpu10I CC'TnA_GC
Cuts at: 0 961 1221
Size: 961 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 886 988 1092 1221
Size: 147 739 102 104 129
Fragments arranged by size:
739 147 129 104 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 89 1106 1221
Size: 89 1017 115
Fragments arranged by size:
1017 115 89
BsaAI yAC'GTr
Cuts at: 0 921 1221
Size: 921 300
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 733 736 927 1221
Size: 733 3 191 294
Fragments arranged by size:
733 294 191 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 625 1221
Size: 397 228 596
Fragments arranged by size:
596 397 228
BsiEI CG_ry'CG
Cuts at: 0 430 1221
Size: 430 791
BsiHKAI G_wGCw'C
Cuts at: 0 1145 1221
Size: 1145 76
BsmI GAATG_Cn'
Cuts at: 0 978 1221
Size: 978 243
BsmBI CGTCTCn'nnnn_
Cuts at: 0 630 1221
Size: 630 591
Bsp1286I G_dGCh'C
Cuts at: 0 135 920 974 1145 1221
Size: 135 785 54 171 76
Fragments arranged by size:
785 171 135 76 54
BsrBI CCG'CTC
Cuts at: 0 645 1221
Size: 645 576
BsrGI T'GTAC_A
Cuts at: 0 302 1221
Size: 302 919
BstXI CCAn_nnnn'nTGG
Cuts at: 0 558 1221
Size: 558 663
BtgI C'CryG_G
Cuts at: 0 392 674 1221
Size: 392 282 547
Fragments arranged by size:
547 392 282
BtsI GCAGTG_nn'
Cuts at: 0 267 386 1221
Size: 267 119 835
Fragments arranged by size:
835 267 119
EaeI y'GGCC_r
Cuts at: 0 671 1155 1221
Size: 671 484 66
Fragments arranged by size:
671 484 66
EarI CTCTTCn'nnn_
Cuts at: 0 526 1221
Size: 526 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 868 1221
Size: 868 353
EcoICRI GAG'CTC
Cuts at: 0 1143 1221
Size: 1143 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 796 1221
Size: 160 636 425
Fragments arranged by size:
636 425 160
EcoNI CCTnn'n_nnAGG
Cuts at: 0 942 1042 1221
Size: 942 100 179
Fragments arranged by size:
942 179 100
EcoO109I rG'GnC_Cy
Cuts at: 0 131 433 1221
Size: 131 302 788
Fragments arranged by size:
788 302 131
EcoRV GAT'ATC
Cuts at: 0 31 1221
Size: 31 1190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 922 954 991 1023 1221
Size: 120 32 770 32 37 32 198
Fragments arranged by size:
770 198 120 37 32 32 32
HincII GTy'rAC
Cuts at: 0 297 1221
Size: 297 924
HindIII A'AGCT_T
Cuts at: 0 114 461 1221
Size: 114 347 760
Fragments arranged by size:
760 347 114
HpaI GTT'AAC
Cuts at: 0 297 1221
Size: 297 924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1221
Size: 563 658
MslI CAynn'nnrTG
Cuts at: 0 391 1221
Size: 391 830
MspA1I CmG'CkG
Cuts at: 0 609 781 840 914 1221
Size: 609 172 59 74 307
Fragments arranged by size:
609 307 172 74 59
NcoI C'CATG_G
Cuts at: 0 392 1221
Size: 392 829
NspI r_CATG'y
Cuts at: 0 481 1071 1221
Size: 481 590 150
Fragments arranged by size:
590 481 150
PciI A'CATG_T
Cuts at: 0 477 1221
Size: 477 744
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1221
Size: 768 453
PfoI T'CCnGG_A
Cuts at: 0 435 767 812 1221
Size: 435 332 45 409
Fragments arranged by size:
435 409 332 45
PmlI CAC'GTG
Cuts at: 0 921 1221
Size: 921 300
PpuMI rG'GwC_Cy
Cuts at: 0 433 1221
Size: 433 788
PspOMI G'GGCC_C
Cuts at: 0 131 1221
Size: 131 1090
PstI C_TGCA'G
Cuts at: 0 1123 1221
Size: 1123 98
PvuII CAG'CTG
Cuts at: 0 609 781 1221
Size: 609 172 440
Fragments arranged by size:
609 440 172
SacI G_AGCT'C
Cuts at: 0 1145 1221
Size: 1145 76
ScaI AGT'ACT
Cuts at: 0 871 1221
Size: 871 350
SfcI C'TryA_G
Cuts at: 0 1119 1221
Size: 1119 102
SmlI C'TyrA_G
Cuts at: 0 126 865 1003 1107 1221
Size: 126 739 138 104 114
Fragments arranged by size:
739 138 126 114 104
SphI G_CATG'C
Cuts at: 0 1071 1221
Size: 1071 150
StuI AGG'CCT
Cuts at: 0 363 889 1221
Size: 363 526 332
Fragments arranged by size:
526 363 332
StyI C'CwwG_G
Cuts at: 0 326 392 1049 1079 1221
Size: 326 66 657 30 142
Fragments arranged by size:
657 326 142 66 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1034 1221
Size: 1034 187
TatI w'GTAC_w
Cuts at: 0 302 690 869 1221
Size: 302 388 179 352
Fragments arranged by size:
388 352 302 179
Tth111I GACn'n_nGTC
Cuts at: 0 217 426 1221
Size: 217 209 795
Fragments arranged by size:
795 217 209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 889 1221
Size: 889 332
Enzymes that do cut:
AflIII ApaI ApoI AvaI BanI BanII BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrGI BstXI
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV Hin4I HincII HindIII HpaI MmeI MslI
MspA1I NcoI NspI PciI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI ScaI SfcI SmlI SphI
StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI
BplI BsaBI BsaHI BsaWI BsaXI BseYI BsiWI BspEI
BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoRI FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI XbaI XhoI XmaI XmnI ZraI