Restriction map for
pF1KA0838
(Linear) (Six-base) MAPSORT of: KIAA0838.seq Check: 3587 from: 1 to: 2007
KIAA0838.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AclI AA'CG_TT
Cuts at: 0 1129 2007
Size: 1129 878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 835 1370 2007
Size: 464 371 535 637
Fragments arranged by size:
637 535 464 371
AflIII A'CryG_T
Cuts at: 0 338 2007
Size: 338 1669
AhdI GACnn_n'nnGTC
Cuts at: 0 672 2007
Size: 672 1335
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 498 530 2007
Size: 498 32 1477
Fragments arranged by size:
1477 498 32
AlwNI CAG_nnn'CTG
Cuts at: 0 246 402 2007
Size: 246 156 1605
Fragments arranged by size:
1605 246 156
ApaI G_GGCC'C
Cuts at: 0 142 2007
Size: 142 1865
ApoI r'AATT_y
Cuts at: 0 472 763 1060 1161 1541 1807 1910 2007
Size: 472 291 297 101 380 266 103 97
Fragments arranged by size:
472 380 297 291 266 103 101 97
AvaI C'yCGr_G
Cuts at: 0 18 113 274 325 2007
Size: 18 95 161 51 1682
Fragments arranged by size:
1682 161 95 51 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 821 854 2007
Size: 821 33 1153
Fragments arranged by size:
1153 821 33
BanI G'GyrC_C
Cuts at: 0 303 2007
Size: 303 1704
BanII G_rGCy'C
Cuts at: 0 142 200 1348 2007
Size: 142 58 1148 659
Fragments arranged by size:
1148 659 142 58
BbeI G_GCGC'C
Cuts at: 0 307 2007
Size: 307 1700
BbsI GAAGACnn'nnnn_
Cuts at: 0 841 1423 2007
Size: 841 582 584
Fragments arranged by size:
841 584 582
BclI T'GATC_A
Cuts at: 0 1641 2007
Size: 1641 366
BfrBI ATG'CAT
Cuts at: 0 1380 2007
Size: 1380 627
BglI GCCn_nnn'nGGC
Cuts at: 0 301 2007
Size: 301 1706
BglII A'GATC_T
Cuts at: 0 233 591 2007
Size: 233 358 1416
Fragments arranged by size:
1416 358 233
Bme1580I G_kGCm'C
Cuts at: 0 84 142 2007
Size: 84 58 1865
Fragments arranged by size:
1865 84 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 86 2007
Size: 86 1921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 845 1028 1466 1709 2007
Size: 746 99 183 438 243 298
Fragments arranged by size:
746 438 298 243 183 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1928 2007
Size: 1928 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 1508 2007
Size: 1508 499
BsaHI Gr'CG_yC
Cuts at: 0 108 304 2007
Size: 108 196 1703
Fragments arranged by size:
1703 196 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 749 1001 1031 2007
Size: 719 30 252 30 976
Fragments arranged by size:
976 719 252 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1496 1499 2007
Size: 1496 3 508
Fragments arranged by size:
1496 508 3
BseYI C'CCAG_C
Cuts at: 0 246 1453 2007
Size: 246 1207 554
Fragments arranged by size:
1207 554 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1685 1867 2007
Size: 1685 182 140
Fragments arranged by size:
1685 182 140
BsiEI CG_ry'CG
Cuts at: 0 49 130 134 2007
Size: 49 81 4 1873
Fragments arranged by size:
1873 81 49 4
BsiHKAI G_wGCw'C
Cuts at: 0 262 1255 2007
Size: 262 993 752
Fragments arranged by size:
993 752 262
BsmI GAATG_Cn'
Cuts at: 0 816 1380 1460 2007
Size: 816 564 80 547
Fragments arranged by size:
816 564 547 80
BsmBI CGTCTCn'nnnn_
Cuts at: 0 325 2007
Size: 325 1682
Bsp1286I G_dGCh'C
Cuts at: 0 84 142 200 262 1255 1348 2007
Size: 84 58 58 62 993 93 659
Fragments arranged by size:
993 659 93 84 62 58 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 2007
Size: 44 1963
BsrDI GCAATG_nn'
Cuts at: 0 881 2007
Size: 881 1126
BsrFI r'CCGG_y
Cuts at: 0 55 104 129 189 2007
Size: 55 49 25 60 1818
Fragments arranged by size:
1818 60 55 49 25
BsrGI T'GTAC_A
Cuts at: 0 796 2007
Size: 796 1211
BstBI TT'CG_AA
Cuts at: 0 1359 1707 2007
Size: 1359 348 300
Fragments arranged by size:
1359 348 300
BstEII G'GTnAC_C
Cuts at: 0 93 2007
Size: 93 1914
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1287 1485 2007
Size: 1287 198 522
Fragments arranged by size:
1287 522 198
BstYI r'GATC_y
Cuts at: 0 233 591 2007
Size: 233 358 1416
Fragments arranged by size:
1416 358 233
Bsu36I CC'TnA_GG
Cuts at: 0 375 2007
Size: 375 1632
BtgI C'CryG_G
Cuts at: 0 134 166 1868 2007
Size: 134 32 1702 139
Fragments arranged by size:
1702 139 134 32
BtsI GCAGTG_nn'
Cuts at: 0 1879 2007
Size: 1879 128
ClaI AT'CG_AT
Cuts at: 0 918 2007
Size: 918 1089
DraI TTT'AAA
Cuts at: 0 600 1908 2007
Size: 600 1308 99
Fragments arranged by size:
1308 600 99
DraIII CAC_nnn'GTG
Cuts at: 0 101 2007
Size: 101 1906
EaeI y'GGCC_r
Cuts at: 0 127 131 187 758 2007
Size: 127 4 56 571 1249
Fragments arranged by size:
1249 571 127 56 4
EagI C'GGCC_G
Cuts at: 0 127 131 2007
Size: 127 4 1876
Fragments arranged by size:
1876 127 4
EarI CTCTTCn'nnn_
Cuts at: 0 560 1623 2007
Size: 560 1063 384
Fragments arranged by size:
1063 560 384
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 2007
Size: 208 1799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 746 835 845 1028 1370 1466 1709
Size: 464 282 89 10 183 342 96 243
Cuts at: 1709 2007
Size: 298
Fragments arranged by size:
464 342 298 282 243 183 96 89
10
EcoO109I rG'GnC_Cy
Cuts at: 0 33 210 2007
Size: 33 177 1797
Fragments arranged by size:
1797 177 33
EcoRI G'AATT_C
Cuts at: 0 1541 2007
Size: 1541 466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1733 2007
Size: 1733 274
FseI GG_CCGG'CC
Cuts at: 0 133 2007
Size: 133 1874
HaeII r_GCGC'y
Cuts at: 0 307 2007
Size: 307 1700
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 658 690 2007
Size: 249 32 377 32 1317
Fragments arranged by size:
1317 377 249 32 32
HincII GTy'rAC
Cuts at: 0 632 2007
Size: 632 1375
KasI G'GCGC_C
Cuts at: 0 303 2007
Size: 303 1704
MfeI C'AATT_G
Cuts at: 0 437 1013 2007
Size: 437 576 994
Fragments arranged by size:
994 576 437
MluI A'CGCG_T
Cuts at: 0 338 2007
Size: 338 1669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 1092 2007
Size: 906 186 915
Fragments arranged by size:
915 906 186
MscI TGG'CCA
Cuts at: 0 760 2007
Size: 760 1247
MslI CAynn'nnrTG
Cuts at: 0 692 920 1216 1261 1385 1586 1867 1898
Size: 692 228 296 45 124 201 281 31
Cuts at: 1898 2007
Size: 109
Fragments arranged by size:
692 296 281 228 201 124 109 45
31
MspA1I CmG'CkG
Cuts at: 0 136 292 1248 1743 2007
Size: 136 156 956 495 264
Fragments arranged by size:
956 495 264 156 136
NaeI GCC'GGC
Cuts at: 0 57 106 131 191 2007
Size: 57 49 25 60 1816
Fragments arranged by size:
1816 60 57 49 25
NarI GG'CG_CC
Cuts at: 0 304 2007
Size: 304 1703
NcoI C'CATG_G
Cuts at: 0 1868 2007
Size: 1868 139
NgoMIV G'CCGG_C
Cuts at: 0 55 104 129 189 2007
Size: 55 49 25 60 1818
Fragments arranged by size:
1818 60 55 49 25
NsiI A_TGCA'T
Cuts at: 0 1382 2007
Size: 1382 625
NspI r_CATG'y
Cuts at: 0 1395 2007
Size: 1395 612
PflMI CCAn_nnn'nTGG
Cuts at: 0 1327 2007
Size: 1327 680
PpuMI rG'GwC_Cy
Cuts at: 0 33 2007
Size: 33 1974
PspOMI G'GGCC_C
Cuts at: 0 138 2007
Size: 138 1869
PstI C_TGCA'G
Cuts at: 0 1785 2007
Size: 1785 222
PvuII CAG'CTG
Cuts at: 0 1248 2007
Size: 1248 759
SacII CC_GC'GG
Cuts at: 0 137 2007
Size: 137 1870
ScaI AGT'ACT
Cuts at: 0 1340 2007
Size: 1340 667
SfcI C'TryA_G
Cuts at: 0 494 1781 2007
Size: 494 1287 226
Fragments arranged by size:
1287 494 226
SfoI GGC'GCC
Cuts at: 0 305 2007
Size: 305 1702
SgrAI Cr'CCGG_yG
Cuts at: 0 55 2007
Size: 55 1952
SmaI CCC'GGG
Cuts at: 0 276 327 2007
Size: 276 51 1680
Fragments arranged by size:
1680 276 51
SmlI C'TyrA_G
Cuts at: 0 1943 2007
Size: 1943 64
StyI C'CwwG_G
Cuts at: 0 89 314 1844 1868 2007
Size: 89 225 1530 24 139
Fragments arranged by size:
1530 225 139 89 24
TatI w'GTAC_w
Cuts at: 0 796 1338 1934 2007
Size: 796 542 596 73
Fragments arranged by size:
796 596 542 73
Tth111I GACn'n_nGTC
Cuts at: 0 1069 2007
Size: 1069 938
XbaI T'CTAG_A
Cuts at: 0 1756 2007
Size: 1756 251
XmaI C'CCGG_G
Cuts at: 0 274 325 2007
Size: 274 51 1682
Fragments arranged by size:
1682 274 51
XmnI GAAnn'nnTTC
Cuts at: 0 1355 2007
Size: 1355 652
Enzymes that do cut:
AclI AcuI AflIII AhdI AloI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbeI BbsI BclI BfrBI
BglI BglII Bme1580I BplI BpmI BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI DraI DraIII EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI
HaeII Hin4I HincII KasI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PflMI PpuMI PspOMI PstI PvuII SacII ScaI SfcI
SfoI SgrAI SmaI SmlI StyI TatI Tth111I XbaI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BamHI BbvCI
BcgI BciVI BlpI BmgBI BmrI BmtI Bpu10I BsaAI
BsaBI BsaWI BsiWI BspEI BspHI BsrBI BssHII BssSI
BstAPI BstZ17I BtgZI CspCI DrdI EcoICRI EcoNI EcoRV
FspI FspAI HindIII HpaI KpnI NdeI NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI SexAI SfiI SgfI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI XcmI
XhoI ZraI