Restriction map for
pF1KA0834
(Linear) (Six-base) MAPSORT of: KIAA0834.seq Check: 1424 from: 1 to: 1353
KIAA0834.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 886 1353
Size: 886 467
AccI GT'mk_AC
Cuts at: 0 88 392 1353
Size: 88 304 961
Fragments arranged by size:
961 304 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1353
Size: 454 899
AflIII A'CryG_T
Cuts at: 0 887 1238 1353
Size: 887 351 115
Fragments arranged by size:
887 351 115
AlwNI CAG_nnn'CTG
Cuts at: 0 666 1285 1353
Size: 666 619 68
Fragments arranged by size:
666 619 68
ApaLI G'TGCA_C
Cuts at: 0 583 1353
Size: 583 770
ApoI r'AATT_y
Cuts at: 0 328 1353
Size: 328 1025
BanII G_rGCy'C
Cuts at: 0 38 1353
Size: 38 1315
BbsI GAAGACnn'nnnn_
Cuts at: 0 1235 1353
Size: 1235 118
BclI T'GATC_A
Cuts at: 0 119 440 734 1353
Size: 119 321 294 619
Fragments arranged by size:
619 321 294 119
BglI GCCn_nnn'nGGC
Cuts at: 0 1218 1353
Size: 1218 135
BlpI GC'TnA_GC
Cuts at: 0 1102 1353
Size: 1102 251
Bme1580I G_kGCm'C
Cuts at: 0 587 1353
Size: 587 766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 827 1040 1307 1353
Size: 285 542 213 267 46
Fragments arranged by size:
542 285 267 213 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1206 1353
Size: 1206 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 704 1353
Size: 704 649
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 793 823 1117 1147 1353
Size: 793 30 294 30 206
Fragments arranged by size:
793 294 206 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 252 1353
Size: 252 1101
BseYI C'CCAG_C
Cuts at: 0 307 1353
Size: 307 1046
BsiHKAI G_wGCw'C
Cuts at: 0 181 587 1353
Size: 181 406 766
Fragments arranged by size:
766 406 181
BsmI GAATG_Cn'
Cuts at: 0 225 1353
Size: 225 1128
BsmBI CGTCTCn'nnnn_
Cuts at: 0 550 1353
Size: 550 803
Bsp1286I G_dGCh'C
Cuts at: 0 38 181 587 1353
Size: 38 143 406 766
Fragments arranged by size:
766 406 143 38
BspHI T'CATG_A
Cuts at: 0 534 1353
Size: 534 819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 886 1217 1353
Size: 886 331 136
Fragments arranged by size:
886 331 136
BsrDI GCAATG_nn'
Cuts at: 0 27 1353
Size: 27 1326
BsrGI T'GTAC_A
Cuts at: 0 1064 1353
Size: 1064 289
BssSI C'ACGA_G
Cuts at: 0 1192 1353
Size: 1192 161
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1180 1353
Size: 1180 173
BstEII G'GTnAC_C
Cuts at: 0 822 1353
Size: 822 531
BstXI CCAn_nnnn'nTGG
Cuts at: 0 819 1223 1353
Size: 819 404 130
Fragments arranged by size:
819 404 130
BstYI r'GATC_y
Cuts at: 0 376 1353
Size: 376 977
BstZ17I GTA'TAC
Cuts at: 0 393 1353
Size: 393 960
Bsu36I CC'TnA_GG
Cuts at: 0 143 1353
Size: 143 1210
BtgI C'CryG_G
Cuts at: 0 1216 1353
Size: 1216 137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 864 899 1353
Size: 864 35 454
Fragments arranged by size:
864 454 35
DraI TTT'AAA
Cuts at: 0 500 1353
Size: 500 853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 454 827 1040 1307 1353
Size: 285 169 373 213 267 46
Fragments arranged by size:
373 285 267 213 169 46
EcoO109I rG'GnC_Cy
Cuts at: 0 1125 1353
Size: 1125 228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 511 1353
Size: 511 842
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1353
Size: 1117 32 204
Fragments arranged by size:
1117 204 32
HindIII A'AGCT_T
Cuts at: 0 488 1353
Size: 488 865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1353
Size: 836 517
MslI CAynn'nnrTG
Cuts at: 0 69 82 1221 1237 1353
Size: 69 13 1139 16 116
Fragments arranged by size:
1139 116 69 16 13
NdeI CA'TA_TG
Cuts at: 0 348 1353
Size: 348 1005
NspI r_CATG'y
Cuts at: 0 517 891 1242 1299 1353
Size: 517 374 351 57 54
Fragments arranged by size:
517 374 351 57 54
PasI CC'CwG_GG
Cuts at: 0 619 1353
Size: 619 734
PciI A'CATG_T
Cuts at: 0 887 1238 1353
Size: 887 351 115
Fragments arranged by size:
887 351 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 1285 1353
Size: 108 1177 68
Fragments arranged by size:
1177 108 68
PfoI T'CCnGG_A
Cuts at: 0 942 1353
Size: 942 411
PpuMI rG'GwC_Cy
Cuts at: 0 1125 1353
Size: 1125 228
PstI C_TGCA'G
Cuts at: 0 33 452 1353
Size: 33 419 901
Fragments arranged by size:
901 419 33
SfcI C'TryA_G
Cuts at: 0 29 386 448 1353
Size: 29 357 62 905
Fragments arranged by size:
905 357 62 29
SmlI C'TyrA_G
Cuts at: 0 1185 1353
Size: 1185 168
SphI G_CATG'C
Cuts at: 0 1299 1353
Size: 1299 54
SspI AAT'ATT
Cuts at: 0 871 980 1353
Size: 871 109 373
Fragments arranged by size:
871 373 109
StyI C'CwwG_G
Cuts at: 0 551 924 1353
Size: 551 373 429
Fragments arranged by size:
551 429 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 760 1353
Size: 760 593
TatI w'GTAC_w
Cuts at: 0 602 1064 1353
Size: 602 462 289
Fragments arranged by size:
602 462 289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 793 825 1353
Size: 793 32 528
Fragments arranged by size:
793 528 32
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaLI ApoI BanII
BbsI BclI BglI BlpI Bme1580I BpmI BpuEI BsaI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI CspCI DraI Eco57MI EcoO109I FalI
Hin4I HindIII MmeI MslI NdeI NspI PasI PciI
PflMI PfoI PpuMI PstI SfcI SmlI SphI SspI
StyI TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BstBI BtgZI BtsI ClaI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI