Restriction map for
pF1KSDA0832
(Linear) (Six-base) MAPSORT of: KIAA0832.seq Check: 602 from: 1 to: 1374
KIAA0832.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AccI GT'mk_AC
Cuts at: 0 941 1374
Size: 941 433
Acc65I G'GTAC_C
Cuts at: 0 410 1374
Size: 410 964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 1156 1374
Size: 167 989 218
Fragments arranged by size:
989 218 167
AfeI AGC'GCT
Cuts at: 0 1193 1374
Size: 1193 181
AgeI A'CCGG_T
Cuts at: 0 944 1374
Size: 944 430
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 189 221 1374
Size: 189 32 1153
Fragments arranged by size:
1153 189 32
ApoI r'AATT_y
Cuts at: 0 920 1093 1374
Size: 920 173 281
Fragments arranged by size:
920 281 173
BanI G'GyrC_C
Cuts at: 0 410 1374
Size: 410 964
BbsI GAAGACnn'nnnn_
Cuts at: 0 1007 1232 1374
Size: 1007 225 142
Fragments arranged by size:
1007 225 142
BbvCI CC'TCA_GC
Cuts at: 0 671 1374
Size: 671 703
BglI GCCn_nnn'nGGC
Cuts at: 0 176 445 1374
Size: 176 269 929
Fragments arranged by size:
929 269 176
BglII A'GATC_T
Cuts at: 0 743 1374
Size: 743 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 1374
Size: 297 1077
Bpu10I CC'TnA_GC
Cuts at: 0 671 1374
Size: 671 703
BsaI GGTCTCn'nnnn_
Cuts at: 0 953 1374
Size: 953 421
BsaAI yAC'GTr
Cuts at: 0 610 1374
Size: 610 764
BsaHI Gr'CG_yC
Cuts at: 0 188 1374
Size: 188 1186
BsaWI w'CCGG_w
Cuts at: 0 735 944 1374
Size: 735 209 430
Fragments arranged by size:
735 430 209
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 189 219 1250 1280 1374
Size: 189 30 1031 30 94
Fragments arranged by size:
1031 189 94 30 30
BseYI C'CCAG_C
Cuts at: 0 135 1374
Size: 135 1239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1315 1374
Size: 25 1290 59
Fragments arranged by size:
1290 59 25
BsiEI CG_ry'CG
Cuts at: 0 819 1374
Size: 819 555
BsmI GAATG_Cn'
Cuts at: 0 448 1374
Size: 448 926
BspHI T'CATG_A
Cuts at: 0 554 1374
Size: 554 820
BsrFI r'CCGG_y
Cuts at: 0 944 1374
Size: 944 430
BstXI CCAn_nnnn'nTGG
Cuts at: 0 172 1374
Size: 172 1202
BstYI r'GATC_y
Cuts at: 0 330 743 1374
Size: 330 413 631
Fragments arranged by size:
631 413 330
BstZ17I GTA'TAC
Cuts at: 0 942 1374
Size: 942 432
Bsu36I CC'TnA_GG
Cuts at: 0 1270 1374
Size: 1270 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 1374
Size: 384 990
BtsI GCAGTG_nn'
Cuts at: 0 1258 1374
Size: 1258 116
DraI TTT'AAA
Cuts at: 0 566 1374
Size: 566 808
DraIII CAC_nnn'GTG
Cuts at: 0 805 1374
Size: 805 569
DrdI GACnn_nn'nnGTC
Cuts at: 0 766 1374
Size: 766 608
EaeI y'GGCC_r
Cuts at: 0 219 812 1212 1374
Size: 219 593 400 162
Fragments arranged by size:
593 400 219 162
EarI CTCTTCn'nnn_
Cuts at: 0 47 186 1374
Size: 47 139 1188
Fragments arranged by size:
1188 139 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 898 1374
Size: 898 476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 297 1156 1374
Size: 167 130 859 218
Fragments arranged by size:
859 218 167 130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 468 1374
Size: 468 906
HaeII r_GCGC'y
Cuts at: 0 1195 1374
Size: 1195 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1374
Size: 295 32 1047
Fragments arranged by size:
1047 295 32
HincII GTy'rAC
Cuts at: 0 159 1374
Size: 159 1215
HindIII A'AGCT_T
Cuts at: 0 1168 1374
Size: 1168 206
KpnI G_GTAC'C
Cuts at: 0 414 1374
Size: 414 960
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1340 1374
Size: 1340 34
MscI TGG'CCA
Cuts at: 0 221 1214 1374
Size: 221 993 160
Fragments arranged by size:
993 221 160
MspA1I CmG'CkG
Cuts at: 0 101 488 675 1053 1374
Size: 101 387 187 378 321
Fragments arranged by size:
387 378 321 187 101
NspI r_CATG'y
Cuts at: 0 357 579 1374
Size: 357 222 795
Fragments arranged by size:
795 357 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 1221 1374
Size: 1221 153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 1374
Size: 189 32 1153
Fragments arranged by size:
1153 189 32
PshAI GACnn'nnGTC
Cuts at: 0 1232 1374
Size: 1232 142
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 643 675 1374
Size: 643 32 699
Fragments arranged by size:
699 643 32
PstI C_TGCA'G
Cuts at: 0 67 905 1052 1199 1374
Size: 67 838 147 147 175
Fragments arranged by size:
838 175 147 147 67
PvuII CAG'CTG
Cuts at: 0 101 488 675 1053 1374
Size: 101 387 187 378 321
Fragments arranged by size:
387 378 321 187 101
SapI GCTCTTCn'nnn_
Cuts at: 0 47 186 1374
Size: 47 139 1188
Fragments arranged by size:
1188 139 47
SfcI C'TryA_G
Cuts at: 0 63 201 901 1048 1112 1195 1374
Size: 63 138 700 147 64 83 179
Fragments arranged by size:
700 179 147 138 83 64 63
SphI G_CATG'C
Cuts at: 0 579 1374
Size: 579 795
StuI AGG'CCT
Cuts at: 0 1022 1374
Size: 1022 352
StyI C'CwwG_G
Cuts at: 0 930 1286 1367 1374
Size: 930 356 81 7
Fragments arranged by size:
930 356 81 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 833 1374
Size: 833 541
TatI w'GTAC_w
Cuts at: 0 626 1374
Size: 626 748
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 1250 1282 1374
Size: 79 32 1139 32 92
Fragments arranged by size:
1139 92 79 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 154 1374
Size: 154 1220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 169 172 876 1374
Size: 169 3 704 498
Fragments arranged by size:
704 498 169 3
XmnI GAAnn'nnTTC
Cuts at: 0 56 1160 1374
Size: 56 1104 214
Fragments arranged by size:
1104 214 56
Enzymes that do cut:
AccI Acc65I AcuI AfeI AgeI AloI ApoI BanI
BbsI BbvCI BglI BglII BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsmI BspHI
BsrFI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI DraI
DraIII DrdI EaeI EarI EciI Eco57MI FalI HaeII
Hin4I HincII HindIII KpnI MmeI MscI MspA1I NspI
PflMI PpiI PshAI PsrI PstI PvuII SapI SfcI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BcgI BciVI BclI BfrBI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaBI
BseRI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgI
ClaI CspCI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HpaI KasI MfeI MluI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmaI ZraI