Restriction map for
pF1KA0830
(Linear) (Six-base) MAPSORT of: KIAA0830.seq Check: 4650 from: 1 to: 1500
KIAA0830.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1089 1500
Size: 1089 411
AccI GT'mk_AC
Cuts at: 0 937 1500
Size: 937 563
Acc65I G'GTAC_C
Cuts at: 0 1414 1500
Size: 1414 86
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1081 1279 1500
Size: 745 336 198 221
Fragments arranged by size:
745 336 221 198
AfeI AGC'GCT
Cuts at: 0 190 1500
Size: 190 1310
AleI CACnn'nnGTG
Cuts at: 0 358 1500
Size: 358 1142
AlwNI CAG_nnn'CTG
Cuts at: 0 646 768 1500
Size: 646 122 732
Fragments arranged by size:
732 646 122
ApoI r'AATT_y
Cuts at: 0 483 1500
Size: 483 1017
AseI AT'TA_AT
Cuts at: 0 335 1033 1106 1500
Size: 335 698 73 394
Fragments arranged by size:
698 394 335 73
AvaI C'yCGr_G
Cuts at: 0 212 719 1500
Size: 212 507 781
Fragments arranged by size:
781 507 212
BamHI G'GATC_C
Cuts at: 0 1241 1500
Size: 1241 259
BanI G'GyrC_C
Cuts at: 0 4 268 1414 1500
Size: 4 264 1146 86
Fragments arranged by size:
1146 264 86 4
BanII G_rGCy'C
Cuts at: 0 1141 1233 1500
Size: 1141 92 267
Fragments arranged by size:
1141 267 92
BbeI G_GCGC'C
Cuts at: 0 272 1500
Size: 272 1228
BbsI GAAGACnn'nnnn_
Cuts at: 0 587 1500
Size: 587 913
BbvCI CC'TCA_GC
Cuts at: 0 785 1500
Size: 785 715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 18 52 1500
Size: 18 34 1448
Fragments arranged by size:
1448 34 18
BglI GCCn_nnn'nGGC
Cuts at: 0 135 1379 1500
Size: 135 1244 121
Fragments arranged by size:
1244 135 121
BglII A'GATC_T
Cuts at: 0 161 756 1500
Size: 161 595 744
Fragments arranged by size:
744 595 161
BlpI GC'TnA_GC
Cuts at: 0 185 1140 1500
Size: 185 955 360
Fragments arranged by size:
955 360 185
Bme1580I G_kGCm'C
Cuts at: 0 7 611 1500
Size: 7 604 889
Fragments arranged by size:
889 604 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1378 1500
Size: 1378 122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 956 1500
Size: 956 544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 935 1500
Size: 704 231 565
Fragments arranged by size:
704 565 231
Bpu10I CC'TnA_GC
Cuts at: 0 445 785 1500
Size: 445 340 715
Fragments arranged by size:
715 445 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1189 1500
Size: 343 846 311
Fragments arranged by size:
846 343 311
BsaAI yAC'GTr
Cuts at: 0 156 1500
Size: 156 1344
BsaHI Gr'CG_yC
Cuts at: 0 269 1500
Size: 269 1231
BsaWI w'CCGG_w
Cuts at: 0 1238 1500
Size: 1238 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 1500
Size: 424 30 1046
Fragments arranged by size:
1046 424 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 340 701 1225 1500
Size: 91 249 361 524 275
Fragments arranged by size:
524 361 275 249 91
BseYI C'CCAG_C
Cuts at: 0 24 46 692 1500
Size: 24 22 646 808
Fragments arranged by size:
808 646 24 22
BsiEI CG_ry'CG
Cuts at: 0 146 1500
Size: 146 1354
BsiHKAI G_wGCw'C
Cuts at: 0 1205 1500
Size: 1205 295
BsmI GAATG_Cn'
Cuts at: 0 1187 1500
Size: 1187 313
Bsp1286I G_dGCh'C
Cuts at: 0 7 611 1141 1205 1233 1500
Size: 7 604 530 64 28 267
Fragments arranged by size:
604 530 267 64 28 7
BspEI T'CCGG_A
Cuts at: 0 1238 1500
Size: 1238 262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1089 1500
Size: 1089 411
BsrDI GCAATG_nn'
Cuts at: 0 1001 1361 1500
Size: 1001 360 139
Fragments arranged by size:
1001 360 139
BsrFI r'CCGG_y
Cuts at: 0 62 85 1500
Size: 62 23 1415
Fragments arranged by size:
1415 62 23
BsrGI T'GTAC_A
Cuts at: 0 1325 1500
Size: 1325 175
BssHII G'CGCG_C
Cuts at: 0 10 171 1500
Size: 10 161 1329
Fragments arranged by size:
1329 161 10
BssSI C'ACGA_G
Cuts at: 0 67 1500
Size: 67 1433
BstYI r'GATC_y
Cuts at: 0 161 756 1241 1500
Size: 161 595 485 259
Fragments arranged by size:
595 485 259 161
BtgI C'CryG_G
Cuts at: 0 698 1331 1500
Size: 698 633 169
Fragments arranged by size:
698 633 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 294 1500
Size: 294 1206
BtsI GCAGTG_nn'
Cuts at: 0 456 579 1390 1403 1500
Size: 456 123 811 13 97
Fragments arranged by size:
811 456 123 97 13
ClaI AT'CG_AT
Cuts at: 0 302 1500
Size: 302 1198
DraIII CAC_nnn'GTG
Cuts at: 0 568 1500
Size: 568 932
EaeI y'GGCC_r
Cuts at: 0 143 464 1163 1214 1310 1500
Size: 143 321 699 51 96 190
Fragments arranged by size:
699 321 190 143 96 51
EagI C'GGCC_G
Cuts at: 0 143 1500
Size: 143 1357
EarI CTCTTCn'nnn_
Cuts at: 0 40 1169 1500
Size: 40 1129 331
Fragments arranged by size:
1129 331 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 745 935 1081 1279 1500
Size: 704 41 190 146 198 221
Fragments arranged by size:
704 221 198 190 146 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 686 1432 1500
Size: 325 361 746 68
Fragments arranged by size:
746 361 325 68
EcoO109I rG'GnC_Cy
Cuts at: 0 122 1500
Size: 122 1378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 402 1500
Size: 402 1098
HaeII r_GCGC'y
Cuts at: 0 192 272 1340 1500
Size: 192 80 1068 160
Fragments arranged by size:
1068 192 160 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 891 923 1500
Size: 891 32 577
Fragments arranged by size:
891 577 32
HincII GTy'rAC
Cuts at: 0 858 1500
Size: 858 642
HindIII A'AGCT_T
Cuts at: 0 1021 1500
Size: 1021 479
HpaI GTT'AAC
Cuts at: 0 858 1500
Size: 858 642
KasI G'GCGC_C
Cuts at: 0 268 1500
Size: 268 1232
KpnI G_GTAC'C
Cuts at: 0 1418 1500
Size: 1418 82
MfeI C'AATT_G
Cuts at: 0 1346 1500
Size: 1346 154
MscI TGG'CCA
Cuts at: 0 466 1165 1216 1312 1500
Size: 466 699 51 96 188
Fragments arranged by size:
699 466 188 96 51
MslI CAynn'nnrTG
Cuts at: 0 226 358 1500
Size: 226 132 1142
Fragments arranged by size:
1142 226 132
MspA1I CmG'CkG
Cuts at: 0 789 1500
Size: 789 711
NaeI GCC'GGC
Cuts at: 0 64 87 1500
Size: 64 23 1413
Fragments arranged by size:
1413 64 23
NarI GG'CG_CC
Cuts at: 0 269 1500
Size: 269 1231
NcoI C'CATG_G
Cuts at: 0 698 1331 1500
Size: 698 633 169
Fragments arranged by size:
698 633 169
NgoMIV G'CCGG_C
Cuts at: 0 62 85 1500
Size: 62 23 1415
Fragments arranged by size:
1415 62 23
PasI CC'CwG_GG
Cuts at: 0 1419 1500
Size: 1419 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 568 1500
Size: 568 932
PfoI T'CCnGG_A
Cuts at: 0 509 845 866 1500
Size: 509 336 21 634
Fragments arranged by size:
634 509 336 21
PmlI CAC'GTG
Cuts at: 0 156 1500
Size: 156 1344
PpuMI rG'GwC_Cy
Cuts at: 0 122 1500
Size: 122 1378
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 223 255 869 901 1500
Size: 223 32 614 32 599
Fragments arranged by size:
614 599 223 32 32
PvuII CAG'CTG
Cuts at: 0 789 1500
Size: 789 711
SanDI GG'GwC_CC
Cuts at: 0 122 1500
Size: 122 1378
SfcI C'TryA_G
Cuts at: 0 204 618 1500
Size: 204 414 882
Fragments arranged by size:
882 414 204
SfoI GGC'GCC
Cuts at: 0 270 1500
Size: 270 1230
SmaI CCC'GGG
Cuts at: 0 214 721 1500
Size: 214 507 779
Fragments arranged by size:
779 507 214
SmlI C'TyrA_G
Cuts at: 0 322 1204 1500
Size: 322 882 296
Fragments arranged by size:
882 322 296
StyI C'CwwG_G
Cuts at: 0 698 1331 1374 1500
Size: 698 633 43 126
Fragments arranged by size:
698 633 126 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1411 1500
Size: 1411 89
TatI w'GTAC_w
Cuts at: 0 230 1325 1500
Size: 230 1095 175
Fragments arranged by size:
1095 230 175
Tth111I GACn'n_nGTC
Cuts at: 0 501 1500
Size: 501 999
XmaI C'CCGG_G
Cuts at: 0 212 719 1500
Size: 212 507 781
Fragments arranged by size:
781 507 212
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlwNI ApoI
AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstYI BtgI BtgZI BtsI ClaI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PfoI PmlI PpuMI PsrI PvuII SanDI SfcI
SfoI SmaI SmlI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AsiSI AvrII BaeI BciVI BclI
BfrBI BmgBI BmtI BsaI BsaBI BsgI BsiWI BsmBI
BspHI BsrBI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI MluI MmeI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XcmI XhoI XmnI ZraI