Restriction map for
pF1KA0810
(Linear) (Six-base) MAPSORT of: KIAA0810.seq Check: 9907 from: 1 to: 2436
KIAA0810.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1173 2436
Size: 1173 1263
AccI GT'mk_AC
Cuts at: 0 2392 2436
Size: 2392 44
Acc65I G'GTAC_C
Cuts at: 0 881 2108 2436
Size: 881 1227 328
Fragments arranged by size:
1227 881 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 268 1138 2436
Size: 74 194 870 1298
Fragments arranged by size:
1298 870 194 74
AfeI AGC'GCT
Cuts at: 0 237 2436
Size: 237 2199
AflIII A'CryG_T
Cuts at: 0 20 62 317 1091 1560 2436
Size: 20 42 255 774 469 876
Fragments arranged by size:
876 774 469 255 42 20
AleI CACnn'nnGTG
Cuts at: 0 1217 2436
Size: 1217 1219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 1883 2436
Size: 604 1279 553
Fragments arranged by size:
1279 604 553
AlwNI CAG_nnn'CTG
Cuts at: 0 560 759 850 1128 1257 1494 1680 1755
Size: 560 199 91 278 129 237 186 75
Cuts at: 1755 2265 2319 2436
Size: 510 54 117
Fragments arranged by size:
560 510 278 237 199 186 129 117
91 75 54
ApaI G_GGCC'C
Cuts at: 0 620 2436
Size: 620 1816
AvaI C'yCGr_G
Cuts at: 0 613 1265 1335 2436
Size: 613 652 70 1101
Fragments arranged by size:
1101 652 613 70
BamHI G'GATC_C
Cuts at: 0 2015 2436
Size: 2015 421
BanI G'GyrC_C
Cuts at: 0 214 226 680 881 932 2108 2436
Size: 214 12 454 201 51 1176 328
Fragments arranged by size:
1176 454 328 214 201 51 12
BanII G_rGCy'C
Cuts at: 0 620 2436
Size: 620 1816
BbsI GAAGACnn'nnnn_
Cuts at: 0 1573 2436
Size: 1573 863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 682 716 929 963 2436
Size: 682 34 213 34 1473
Fragments arranged by size:
1473 682 213 34 34
BclI T'GATC_A
Cuts at: 0 2283 2436
Size: 2283 153
BfrBI ATG'CAT
Cuts at: 0 1059 2436
Size: 1059 1377
Bme1580I G_kGCm'C
Cuts at: 0 620 2436
Size: 620 1816
BmgBI CAC'GTC
Cuts at: 0 335 588 1092 1611 2436
Size: 335 253 504 519 825
Fragments arranged by size:
825 519 504 335 253
BmrI ACTGGGnnnn_n'
Cuts at: 0 30 1056 2385 2436
Size: 30 1026 1329 51
Fragments arranged by size:
1329 1026 51 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 872 1498 1540 2179 2287 2436
Size: 818 54 626 42 639 108 149
Fragments arranged by size:
818 639 626 149 108 54 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1977 2436
Size: 1977 459
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 1730 2436
Size: 1000 730 706
Fragments arranged by size:
1000 730 706
BsaAI yAC'GTr
Cuts at: 0 65 318 1563 1867 2279 2436
Size: 65 253 1245 304 412 157
Fragments arranged by size:
1245 412 304 253 157 65
BsaBI GATnn'nnATC
Cuts at: 0 467 2436
Size: 467 1969
BsaHI Gr'CG_yC
Cuts at: 0 344 1840 2436
Size: 344 1496 596
Fragments arranged by size:
1496 596 344
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 2436
Size: 532 30 1874
Fragments arranged by size:
1874 532 30
BseYI C'CCAG_C
Cuts at: 0 867 2084 2142 2436
Size: 867 1217 58 294
Fragments arranged by size:
1217 867 294 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 2436
Size: 755 1681
BsiEI CG_ry'CG
Cuts at: 0 702 2436
Size: 702 1734
BsiHKAI G_wGCw'C
Cuts at: 0 119 593 1511 2165 2436
Size: 119 474 918 654 271
Fragments arranged by size:
918 654 474 271 119
BsmBI CGTCTCn'nnnn_
Cuts at: 0 103 1310 2436
Size: 103 1207 1126
Fragments arranged by size:
1207 1126 103
Bsp1286I G_dGCh'C
Cuts at: 0 119 593 620 1511 2165 2436
Size: 119 474 27 891 654 271
Fragments arranged by size:
891 654 474 271 119 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 318 727 982 1173 1748 2436
Size: 318 409 255 191 575 688
Fragments arranged by size:
688 575 409 318 255 191
BsrBI CCG'CTC
Cuts at: 0 259 2436
Size: 259 2177
BsrFI r'CCGG_y
Cuts at: 0 1570 2436
Size: 1570 866
BsrGI T'GTAC_A
Cuts at: 0 23 2436
Size: 23 2413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 560 759 1128 1494 1728 1755 2436
Size: 560 199 369 366 234 27 681
Fragments arranged by size:
681 560 369 366 234 199 27
BstBI TT'CG_AA
Cuts at: 0 939 2436
Size: 939 1497
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1216 2436
Size: 1216 1220
BstYI r'GATC_y
Cuts at: 0 1751 2015 2436
Size: 1751 264 421
Fragments arranged by size:
1751 421 264
BstZ17I GTA'TAC
Cuts at: 0 2393 2436
Size: 2393 43
BtgI C'CryG_G
Cuts at: 0 145 605 824 1153 1215 1811 2436
Size: 145 460 219 329 62 596 625
Fragments arranged by size:
625 596 460 329 219 145 62
DraI TTT'AAA
Cuts at: 0 2094 2436
Size: 2094 342
DrdI GACnn_nn'nnGTC
Cuts at: 0 804 1615 2221 2436
Size: 804 811 606 215
Fragments arranged by size:
811 804 606 215
EaeI y'GGCC_r
Cuts at: 0 173 260 821 2436
Size: 173 87 561 1615
Fragments arranged by size:
1615 561 173 87
EarI CTCTTCn'nnn_
Cuts at: 0 982 2436
Size: 982 1454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 268 818 872 1138 1498 1540 2179
Size: 74 194 550 54 266 360 42 639
Cuts at: 2179 2287 2436
Size: 108 149
Fragments arranged by size:
639 550 360 266 194 149 108 74
54 42
EcoO109I rG'GnC_Cy
Cuts at: 0 710 2316 2436
Size: 710 1606 120
Fragments arranged by size:
1606 710 120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2278 2436
Size: 2278 158
FspI TGC'GCA
Cuts at: 0 763 2436
Size: 763 1673
HaeII r_GCGC'y
Cuts at: 0 239 1292 1886 1999 2207 2436
Size: 239 1053 594 113 208 229
Fragments arranged by size:
1053 594 239 229 208 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2281 2313 2436
Size: 2281 32 123
Fragments arranged by size:
2281 123 32
KpnI G_GTAC'C
Cuts at: 0 885 2112 2436
Size: 885 1227 324
Fragments arranged by size:
1227 885 324
MscI TGG'CCA
Cuts at: 0 175 2436
Size: 175 2261
MslI CAynn'nnrTG
Cuts at: 0 1214 1217 2436
Size: 1214 3 1219
Fragments arranged by size:
1219 1214 3
MspA1I CmG'CkG
Cuts at: 0 224 607 1071 1518 1553 1677 1746 2022
Size: 224 383 464 447 35 124 69 276
Cuts at: 2022 2436
Size: 414
Fragments arranged by size:
464 447 414 383 276 224 124 69
35
NcoI C'CATG_G
Cuts at: 0 1215 2436
Size: 1215 1221
NsiI A_TGCA'T
Cuts at: 0 1061 2436
Size: 1061 1375
NspI r_CATG'y
Cuts at: 0 24 188 570 734 1059 2436
Size: 24 164 382 164 325 1377
Fragments arranged by size:
1377 382 325 164 164 24
PasI CC'CwG_GG
Cuts at: 0 192 2103 2436
Size: 192 1911 333
Fragments arranged by size:
1911 333 192
PciI A'CATG_T
Cuts at: 0 20 2436
Size: 20 2416
PflMI CCAn_nnn'nTGG
Cuts at: 0 2352 2436
Size: 2352 84
PfoI T'CCnGG_A
Cuts at: 0 793 2436
Size: 793 1643
PmlI CAC'GTG
Cuts at: 0 318 1563 1867 2436
Size: 318 1245 304 569
Fragments arranged by size:
1245 569 318 304
PpuMI rG'GwC_Cy
Cuts at: 0 710 2436
Size: 710 1726
PspOMI G'GGCC_C
Cuts at: 0 616 2436
Size: 616 1820
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 43 75 1953 1985 2436
Size: 43 32 1878 32 451
Fragments arranged by size:
1878 451 43 32 32
PstI C_TGCA'G
Cuts at: 0 374 556 565 755 1133 1262 1685 1745
Size: 374 182 9 190 378 129 423 60
Cuts at: 1745 1751 2436
Size: 6 685
Fragments arranged by size:
685 423 378 374 190 182 129 60
9 6
PvuII CAG'CTG
Cuts at: 0 1518 1553 1677 1746 2436
Size: 1518 35 124 69 690
Fragments arranged by size:
1518 690 124 69 35
SacII CC_GC'GG
Cuts at: 0 608 2436
Size: 608 1828
SanDI GG'GwC_CC
Cuts at: 0 710 2436
Size: 710 1726
SbfI CC_TGCA'GG
Cuts at: 0 374 2436
Size: 374 2062
ScaI AGT'ACT
Cuts at: 0 1962 2436
Size: 1962 474
SexAI A'CCwGG_T
Cuts at: 0 928 2111 2436
Size: 928 1183 325
Fragments arranged by size:
1183 928 325
SfcI C'TryA_G
Cuts at: 0 370 552 561 751 1129 1249 1258 1681
Size: 370 182 9 190 378 120 9 423
Cuts at: 1681 1741 1747 2436
Size: 60 6 689
Fragments arranged by size:
689 423 378 370 190 182 120 60
9 9 6
SmaI CCC'GGG
Cuts at: 0 615 2436
Size: 615 1821
SmlI C'TyrA_G
Cuts at: 0 1956 2436
Size: 1956 480
SphI G_CATG'C
Cuts at: 0 188 570 1059 2436
Size: 188 382 489 1377
Fragments arranged by size:
1377 489 382 188
StyI C'CwwG_G
Cuts at: 0 1215 2210 2436
Size: 1215 995 226
Fragments arranged by size:
1215 995 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 1299 1783 2156 2436
Size: 202 1097 484 373 280
Fragments arranged by size:
1097 484 373 280 202
TatI w'GTAC_w
Cuts at: 0 23 1960 2436
Size: 23 1937 476
Fragments arranged by size:
1937 476 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1762 1794 2436
Size: 1762 32 642
Fragments arranged by size:
1762 642 32
Tth111I GACn'n_nGTC
Cuts at: 0 2178 2436
Size: 2178 258
XmaI C'CCGG_G
Cuts at: 0 613 2436
Size: 613 1823
XmnI GAAnn'nnTTC
Cuts at: 0 1400 2436
Size: 1400 1036
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApaI AvaI BamHI BanI BanII BbsI BcgI
BclI BfrBI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstBI
BstXI BstYI BstZ17I BtgI DraI DrdI EaeI EarI
Eco57MI EcoO109I FalI FspI HaeII Hin4I KpnI MscI
MslI MspA1I NcoI NsiI NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacII
SanDI SbfI ScaI SexAI SfcI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI
BglI BglII BlpI BmtI BplI Bpu10I BsaWI BseRI
BsiWI BsmI BspEI BspHI BsrDI BssHII BssSI BstEII
Bsu36I BtgZI BtsI ClaI CspCI DraIII EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KasI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PmeI PpiI PshAI
PsiI PspXI PvuI RsrII SacI SalI SapI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XcmI XhoI ZraI