Restriction map for
pF1KA0808
(Linear) (Six-base) MAPSORT of: KIAA0808.seq Check: 5254 from: 1 to: 1578
KIAA0808.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1578
Size: 985 593
AflIII A'CryG_T
Cuts at: 0 116 1578
Size: 116 1462
AgeI A'CCGG_T
Cuts at: 0 1509 1578
Size: 1509 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 581 613 1578
Size: 581 32 965
Fragments arranged by size:
965 581 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1219 1578
Size: 1219 359
ApaI G_GGCC'C
Cuts at: 0 1085 1578
Size: 1085 493
ApaLI G'TGCA_C
Cuts at: 0 250 1578
Size: 250 1328
BamHI G'GATC_C
Cuts at: 0 768 1578
Size: 768 810
BanII G_rGCy'C
Cuts at: 0 782 1085 1578
Size: 782 303 493
Fragments arranged by size:
782 493 303
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 1036 1578
Size: 203 833 542
Fragments arranged by size:
833 542 203
BbvCI CC'TCA_GC
Cuts at: 0 1046 1578
Size: 1046 532
BciVI GTATCCnnnnn_n'
Cuts at: 0 1008 1578
Size: 1008 570
BclI T'GATC_A
Cuts at: 0 1052 1578
Size: 1052 526
BfrBI ATG'CAT
Cuts at: 0 1164 1578
Size: 1164 414
BglI GCCn_nnn'nGGC
Cuts at: 0 1099 1578
Size: 1099 479
Bme1580I G_kGCm'C
Cuts at: 0 254 1085 1578
Size: 254 831 493
Fragments arranged by size:
831 493 254
BmgBI CAC'GTC
Cuts at: 0 1026 1578
Size: 1026 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1246 1578
Size: 8 1238 332
Fragments arranged by size:
1238 332 8
Bpu10I CC'TnA_GC
Cuts at: 0 1046 1578
Size: 1046 532
BsaI GGTCTCn'nnnn_
Cuts at: 0 468 1578
Size: 468 1110
BsaWI w'CCGG_w
Cuts at: 0 1509 1578
Size: 1509 69
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 212 1034 1241 1244 1578
Size: 212 822 207 3 334
Fragments arranged by size:
822 334 212 207 3
BseYI C'CCAG_C
Cuts at: 0 29 369 1084 1578
Size: 29 340 715 494
Fragments arranged by size:
715 494 340 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1509 1578
Size: 1430 79 69
Fragments arranged by size:
1430 79 69
BsiHKAI G_wGCw'C
Cuts at: 0 254 567 1578
Size: 254 313 1011
Fragments arranged by size:
1011 313 254
Bsp1286I G_dGCh'C
Cuts at: 0 254 567 782 1085 1578
Size: 254 313 215 303 493
Fragments arranged by size:
493 313 303 254 215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1344 1578
Size: 1344 234
BsrBI CCG'CTC
Cuts at: 0 1314 1578
Size: 1314 264
BsrDI GCAATG_nn'
Cuts at: 0 600 1472 1578
Size: 600 872 106
Fragments arranged by size:
872 600 106
BsrFI r'CCGG_y
Cuts at: 0 1509 1578
Size: 1509 69
BstBI TT'CG_AA
Cuts at: 0 1060 1578
Size: 1060 518
BstEII G'GTnAC_C
Cuts at: 0 274 1578
Size: 274 1304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 1578
Size: 369 1209
BstYI r'GATC_y
Cuts at: 0 768 1578
Size: 768 810
BtgI C'CryG_G
Cuts at: 0 1077 1578
Size: 1077 501
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 692 1578
Size: 692 886
BtsI GCAGTG_nn'
Cuts at: 0 385 1578
Size: 385 1193
EaeI y'GGCC_r
Cuts at: 0 528 1578
Size: 528 1050
EarI CTCTTCn'nnn_
Cuts at: 0 905 1283 1406 1578
Size: 905 378 123 172
Fragments arranged by size:
905 378 172 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 985 1246 1578
Size: 8 977 261 332
Fragments arranged by size:
977 332 261 8
EcoO109I rG'GnC_Cy
Cuts at: 0 64 1578
Size: 64 1514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 1578
Size: 670 32 876
Fragments arranged by size:
876 670 32
HincII GTy'rAC
Cuts at: 0 1023 1578
Size: 1023 555
MscI TGG'CCA
Cuts at: 0 530 1578
Size: 530 1048
MslI CAynn'nnrTG
Cuts at: 0 121 257 406 668 1240 1578
Size: 121 136 149 262 572 338
Fragments arranged by size:
572 338 262 149 136 121
MspA1I CmG'CkG
Cuts at: 0 40 534 1050 1444 1578
Size: 40 494 516 394 134
Fragments arranged by size:
516 494 394 134 40
NcoI C'CATG_G
Cuts at: 0 1077 1578
Size: 1077 501
NsiI A_TGCA'T
Cuts at: 0 1166 1578
Size: 1166 412
NspI r_CATG'y
Cuts at: 0 120 1299 1551 1578
Size: 120 1179 252 27
Fragments arranged by size:
1179 252 120 27
PciI A'CATG_T
Cuts at: 0 116 1578
Size: 116 1462
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 1467 1578
Size: 246 1221 111
Fragments arranged by size:
1221 246 111
PpuMI rG'GwC_Cy
Cuts at: 0 64 1578
Size: 64 1514
PspOMI G'GGCC_C
Cuts at: 0 1081 1578
Size: 1081 497
PstI C_TGCA'G
Cuts at: 0 495 1537 1578
Size: 495 1042 41
Fragments arranged by size:
1042 495 41
PvuII CAG'CTG
Cuts at: 0 534 1050 1444 1578
Size: 534 516 394 134
Fragments arranged by size:
534 516 394 134
SapI GCTCTTCn'nnn_
Cuts at: 0 905 1578
Size: 905 673
SfcI C'TryA_G
Cuts at: 0 491 1008 1226 1439 1533 1578
Size: 491 517 218 213 94 45
Fragments arranged by size:
517 491 218 213 94 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1099 1578
Size: 1099 479
SphI G_CATG'C
Cuts at: 0 1551 1578
Size: 1551 27
StyI C'CwwG_G
Cuts at: 0 478 1077 1332 1578
Size: 478 599 255 246
Fragments arranged by size:
599 478 255 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1392 1474 1578
Size: 1392 82 104
Fragments arranged by size:
1392 104 82
TatI w'GTAC_w
Cuts at: 0 443 1578
Size: 443 1135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 172 1578
Size: 172 1406
Enzymes that do cut:
AcuI AflIII AgeI AloI AlwNI ApaI ApaLI BamHI
BanII BbsI BbvCI BciVI BclI BfrBI BglI Bme1580I
BmgBI BpmI Bpu10I BsaI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI
EcoO109I Hin4I HincII MscI MslI MspA1I NcoI NsiI
NspI PciI PflMI PpuMI PspOMI PstI PvuII SapI
SfcI SfiI SphI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BcgI BglII BlpI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI