Restriction map for
pF1KA0800
(Linear) (Six-base) MAPSORT of: KIAA0800.seq Check: 1043 from: 1 to: 4521
KIAA0800.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3082 3298 3343 4521
Size: 3082 216 45 1178
Fragments arranged by size:
3082 1178 216 45
Acc65I G'GTAC_C
Cuts at: 0 93 4521
Size: 93 4428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1105 1346 1612 1784 2029 3370 4521
Size: 158 947 241 266 172 245 1341 1151
Fragments arranged by size:
1341 1151 947 266 245 241 172 158
AflIII A'CryG_T
Cuts at: 0 18 1934 4521
Size: 18 1916 2587
Fragments arranged by size:
2587 1916 18
AgeI A'CCGG_T
Cuts at: 0 1231 4521
Size: 1231 3290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1585 2224 4521
Size: 1585 639 2297
Fragments arranged by size:
2297 1585 639
AlwNI CAG_nnn'CTG
Cuts at: 0 2348 2364 2671 4179 4296 4521
Size: 2348 16 307 1508 117 225
Fragments arranged by size:
2348 1508 307 225 117 16
ApaI G_GGCC'C
Cuts at: 0 408 2268 4521
Size: 408 1860 2253
Fragments arranged by size:
2253 1860 408
ApaLI G'TGCA_C
Cuts at: 0 1505 4521
Size: 1505 3016
ApoI r'AATT_y
Cuts at: 0 501 1156 1168 1488 1717 2027 2101 4521
Size: 501 655 12 320 229 310 74 2420
Fragments arranged by size:
2420 655 501 320 310 229 74 12
AseI AT'TA_AT
Cuts at: 0 3824 4521
Size: 3824 697
AvaI C'yCGr_G
Cuts at: 0 407 4521
Size: 407 4114
BamHI G'GATC_C
Cuts at: 0 156 4521
Size: 156 4365
BanI G'GyrC_C
Cuts at: 0 93 937 1896 2758 2944 3218 3331 4521
Size: 93 844 959 862 186 274 113 1190
Fragments arranged by size:
1190 959 862 844 274 186 113 93
BanII G_rGCy'C
Cuts at: 0 41 296 408 1427 2268 4521
Size: 41 255 112 1019 841 2253
Fragments arranged by size:
2253 1019 841 255 112 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 537 1438 1837 4427 4521
Size: 537 901 399 2590 94
Fragments arranged by size:
2590 901 537 399 94
BbvCI CC'TCA_GC
Cuts at: 0 1997 2792 4521
Size: 1997 795 1729
Fragments arranged by size:
1997 1729 795
BclI T'GATC_A
Cuts at: 0 1466 3818 4521
Size: 1466 2352 703
Fragments arranged by size:
2352 1466 703
BfrBI ATG'CAT
Cuts at: 0 271 1296 4521
Size: 271 1025 3225
Fragments arranged by size:
3225 1025 271
BglI GCCn_nnn'nGGC
Cuts at: 0 412 4521
Size: 412 4109
BglII A'GATC_T
Cuts at: 0 713 3651 4521
Size: 713 2938 870
Fragments arranged by size:
2938 870 713
BlpI GC'TnA_GC
Cuts at: 0 35 4521
Size: 35 4486
Bme1580I G_kGCm'C
Cuts at: 0 408 1509 2268 4521
Size: 408 1101 759 2253
Fragments arranged by size:
2253 1101 759 408
BmgBI CAC'GTC
Cuts at: 0 1935 4521
Size: 1935 2586
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 1556 2511 2659 4521
Size: 581 975 955 148 1862
Fragments arranged by size:
1862 975 955 581 148
BmtI G_CTAG'C
Cuts at: 0 1538 2821 4384 4521
Size: 1538 1283 1563 137
Fragments arranged by size:
1563 1538 1283 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 722 1897 3832 4318 4521
Size: 73 649 1175 1935 486 203
Fragments arranged by size:
1935 1175 649 486 203 73
Bpu10I CC'TnA_GC
Cuts at: 0 1997 2792 3113 4521
Size: 1997 795 321 1408
Fragments arranged by size:
1997 1408 795 321
BsaI GGTCTCn'nnnn_
Cuts at: 0 677 4521
Size: 677 3844
BsaBI GATnn'nnATC
Cuts at: 0 179 875 2053 2239 4521
Size: 179 696 1178 186 2282
Fragments arranged by size:
2282 1178 696 186 179
BsaHI Gr'CG_yC
Cuts at: 0 3722 4521
Size: 3722 799
BsaWI w'CCGG_w
Cuts at: 0 1231 4521
Size: 1231 3290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 974 1004 4291 4321 4521
Size: 974 30 3287 30 200
Fragments arranged by size:
3287 974 200 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 2108 3089 3394 4216 4327 4390 4411
Size: 646 1462 981 305 822 111 63 21
Cuts at: 4411 4453 4521
Size: 42 68
Fragments arranged by size:
1462 981 822 646 305 111 68 63
42 21
BseYI C'CCAG_C
Cuts at: 0 210 1737 2961 4521
Size: 210 1527 1224 1560
Fragments arranged by size:
1560 1527 1224 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3321 3467 3728 4521
Size: 3321 146 261 793
Fragments arranged by size:
3321 793 261 146
BsiHKAI G_wGCw'C
Cuts at: 0 41 1427 1509 2159 2213 4521
Size: 41 1386 82 650 54 2308
Fragments arranged by size:
2308 1386 650 82 54 41
BsmI GAATG_Cn'
Cuts at: 0 261 273 1646 3298 3519 4521
Size: 261 12 1373 1652 221 1002
Fragments arranged by size:
1652 1373 1002 261 221 12
Bsp1286I G_dGCh'C
Cuts at: 0 41 296 408 1427 1509 2159 2213 2268
Size: 41 255 112 1019 82 650 54 55
Cuts at: 2268 4521
Size: 2253
Fragments arranged by size:
2253 1019 650 255 112 82 55 54
41
BspHI T'CATG_A
Cuts at: 0 251 1692 4521
Size: 251 1441 2829
Fragments arranged by size:
2829 1441 251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1567 2633 3082 3298 3343 4521
Size: 1567 1066 449 216 45 1178
Fragments arranged by size:
1567 1178 1066 449 216 45
BsrBI CCG'CTC
Cuts at: 0 3315 3740 3877 4521
Size: 3315 425 137 644
Fragments arranged by size:
3315 644 425 137
BsrDI GCAATG_nn'
Cuts at: 0 476 2723 2907 3593 4521
Size: 476 2247 184 686 928
Fragments arranged by size:
2247 928 686 476 184
BsrFI r'CCGG_y
Cuts at: 0 1231 4521
Size: 1231 3290
BsrGI T'GTAC_A
Cuts at: 0 4072 4521
Size: 4072 449
BstBI TT'CG_AA
Cuts at: 0 528 3846 4521
Size: 528 3318 675
Fragments arranged by size:
3318 675 528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1605 3805 4521
Size: 1605 2200 716
Fragments arranged by size:
2200 1605 716
BstYI r'GATC_y
Cuts at: 0 156 713 1951 2354 2525 3651 4521
Size: 156 557 1238 403 171 1126 870
Fragments arranged by size:
1238 1126 870 557 403 171 156
Bsu36I CC'TnA_GG
Cuts at: 0 776 4521
Size: 776 3745
BtgI C'CryG_G
Cuts at: 0 592 1277 2797 2940 4521
Size: 592 685 1520 143 1581
Fragments arranged by size:
1581 1520 685 592 143
BtsI GCAGTG_nn'
Cuts at: 0 64 84 305 2157 2403 2669 4521
Size: 64 20 221 1852 246 266 1852
Fragments arranged by size:
1852 1852 266 246 221 64 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1152 1187 4521
Size: 1152 35 3334
Fragments arranged by size:
3334 1152 35
DraI TTT'AAA
Cuts at: 0 302 4521
Size: 302 4219
DrdI GACnn_nn'nnGTC
Cuts at: 0 185 4521
Size: 185 4336
EaeI y'GGCC_r
Cuts at: 0 1595 2165 4521
Size: 1595 570 2356
Fragments arranged by size:
2356 1595 570
EarI CTCTTCn'nnn_
Cuts at: 0 134 238 609 3962 4193 4208 4521
Size: 134 104 371 3353 231 15 313
Fragments arranged by size:
3353 371 313 231 134 104 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3218 4318 4521
Size: 3218 1100 203
Fragments arranged by size:
3218 1100 203
EcoICRI GAG'CTC
Cuts at: 0 39 1425 4521
Size: 39 1386 3096
Fragments arranged by size:
3096 1386 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 158 722 1105 1346 1612 1784 1897
Size: 73 85 564 383 241 266 172 113
Cuts at: 1897 2029 3370 3832 4318 4521
Size: 132 1341 462 486 203
Fragments arranged by size:
1341 564 486 462 383 266 241 203
172 132 113 85 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4393 4521
Size: 4393 128
EcoO109I rG'GnC_Cy
Cuts at: 0 2265 3440 3840 4521
Size: 2265 1175 400 681
Fragments arranged by size:
2265 1175 681 400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2566 3540 4521
Size: 2566 974 981
Fragments arranged by size:
2566 981 974
FspI TGC'GCA
Cuts at: 0 1178 1573 4521
Size: 1178 395 2948
Fragments arranged by size:
2948 1178 395
HaeII r_GCGC'y
Cuts at: 0 3139 4521
Size: 3139 1382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 974 1006 1936 1943 1968 1975 2875 2907
Size: 974 32 930 7 25 7 900 32
Cuts at: 2907 3724 3756 4408 4440 4521
Size: 817 32 652 32 81
Fragments arranged by size:
974 930 900 817 652 81 32 32
32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 3635 4521
Size: 3635 886
HindIII A'AGCT_T
Cuts at: 0 695 4521
Size: 695 3826
KpnI G_GTAC'C
Cuts at: 0 97 4521
Size: 97 4424
MfeI C'AATT_G
Cuts at: 0 118 1824 2058 4521
Size: 118 1706 234 2463
Fragments arranged by size:
2463 1706 234 118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1919 2216 2915 4289 4521
Size: 1919 297 699 1374 232
Fragments arranged by size:
1919 1374 699 297 232
MscI TGG'CCA
Cuts at: 0 1597 2167 4521
Size: 1597 570 2354
Fragments arranged by size:
2354 1597 570
MslI CAynn'nnrTG
Cuts at: 0 77 1039 2206 2233 3605 3803 4045 4521
Size: 77 962 1167 27 1372 198 242 476
Fragments arranged by size:
1372 1167 962 476 242 198 77 27
MspA1I CmG'CkG
Cuts at: 0 510 1762 2348 2364 2979 3348 4521
Size: 510 1252 586 16 615 369 1173
Fragments arranged by size:
1252 1173 615 586 510 369 16
NcoI C'CATG_G
Cuts at: 0 1277 2940 4521
Size: 1277 1663 1581
Fragments arranged by size:
1663 1581 1277
NdeI CA'TA_TG
Cuts at: 0 273 4521
Size: 273 4248
NheI G'CTAG_C
Cuts at: 0 1534 2817 4380 4521
Size: 1534 1283 1563 141
Fragments arranged by size:
1563 1534 1283 141
NsiI A_TGCA'T
Cuts at: 0 273 1298 4521
Size: 273 1025 3223
Fragments arranged by size:
3223 1025 273
NspI r_CATG'y
Cuts at: 0 22 885 1296 1682 3049 4521
Size: 22 863 411 386 1367 1472
Fragments arranged by size:
1472 1367 863 411 386 22
PasI CC'CwG_GG
Cuts at: 0 926 2808 4521
Size: 926 1882 1713
Fragments arranged by size:
1882 1713 926
PciI A'CATG_T
Cuts at: 0 18 4521
Size: 18 4503
PflMI CCAn_nnn'nTGG
Cuts at: 0 2174 3187 4296 4521
Size: 2174 1013 1109 225
Fragments arranged by size:
2174 1109 1013 225
PfoI T'CCnGG_A
Cuts at: 0 3257 4521
Size: 3257 1264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1990 2022 4521
Size: 1990 32 2499
Fragments arranged by size:
2499 1990 32
PpuMI rG'GwC_Cy
Cuts at: 0 3440 3840 4521
Size: 3440 400 681
Fragments arranged by size:
3440 681 400
PspOMI G'GGCC_C
Cuts at: 0 404 2264 4521
Size: 404 1860 2257
Fragments arranged by size:
2257 1860 404
PstI C_TGCA'G
Cuts at: 0 312 2387 2498 4521
Size: 312 2075 111 2023
Fragments arranged by size:
2075 2023 312 111
PvuII CAG'CTG
Cuts at: 0 510 1762 2348 2364 2979 3348 4521
Size: 510 1252 586 16 615 369 1173
Fragments arranged by size:
1252 1173 615 586 510 369 16
SacI G_AGCT'C
Cuts at: 0 41 1427 4521
Size: 41 1386 3094
Fragments arranged by size:
3094 1386 41
ScaI AGT'ACT
Cuts at: 0 1473 4521
Size: 1473 3048
SfcI C'TryA_G
Cuts at: 0 5 308 489 544 550 665 1955 1967
Size: 5 303 181 55 6 115 1290 12
Cuts at: 1967 2383 2494 2949 3695 4031 4521
Size: 416 111 455 746 336 490
Fragments arranged by size:
1290 746 490 455 416 336 303 181
115 111 55 12 6 5
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 412 4521
Size: 412 4109
SpeI A'CTAG_T
Cuts at: 0 745 4521
Size: 745 3776
SphI G_CATG'C
Cuts at: 0 1296 1682 4521
Size: 1296 386 2839
Fragments arranged by size:
2839 1296 386
SspI AAT'ATT
Cuts at: 0 997 1529 4521
Size: 997 532 2992
Fragments arranged by size:
2992 997 532
StyI C'CwwG_G
Cuts at: 0 1277 2940 4521
Size: 1277 1663 1581
Fragments arranged by size:
1663 1581 1277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 918 1673 2746 4521
Size: 918 755 1073 1775
Fragments arranged by size:
1775 1073 918 755
TatI w'GTAC_w
Cuts at: 0 1188 1471 4072 4521
Size: 1188 283 2601 449
Fragments arranged by size:
2601 1188 449 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1626 1658 3675 3707 3737 3769 3800 3832
Size: 1626 32 2017 32 30 32 31 32
Cuts at: 3832 4521
Size: 689
Fragments arranged by size:
2017 1626 689 32 32 32 32 31
30
Tth111I GACn'n_nGTC
Cuts at: 0 2404 4521
Size: 2404 2117
XbaI T'CTAG_A
Cuts at: 0 4392 4521
Size: 4392 129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 849 4521
Size: 849 3672
XmnI GAAnn'nnTTC
Cuts at: 0 394 4521
Size: 394 4127
Enzymes that do cut:
AarI Acc65I AcuI AflIII AgeI AlfI AlwNI ApaI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI
Bsu36I BtgI BtsI CspCI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NheI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
ScaI SfcI SfiI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AhdI AleI AloI
AscI AsiSI AvrII BaeI BbeI BcgI BciVI BplI
BpuEI BsaAI BsiEI BsiWI BsmBI BspEI BssHII BssSI
BstAPI BstEII BstZ17I BtgZI ClaI DraIII EagI EcoRI
EcoRV FseI FspAI HpaI KasI MluI NaeI NarI
NgoMIV NotI NruI PacI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI
SrfI StuI SwaI XhoI XmaI ZraI