Restriction map for
pF1KA0799
(Linear) (Six-base) MAPSORT of: KIAA0799.seq Check: 7751 from: 1 to: 6174
KIAA0799.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2646 6174
Size: 2646 3528
AatII G_ACGT'C
Cuts at: 0 2096 6174
Size: 2096 4078
AccI GT'mk_AC
Cuts at: 0 996 1184 5252 5945 6174
Size: 996 188 4068 693 229
Fragments arranged by size:
4068 996 693 229 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1357 2934 3071 4261 4678 6174
Size: 1357 1577 137 1190 417 1496
Fragments arranged by size:
1577 1496 1357 1190 417 137
AfeI AGC'GCT
Cuts at: 0 3709 6130 6174
Size: 3709 2421 44
Fragments arranged by size:
3709 2421 44
AflII C'TTAA_G
Cuts at: 0 5026 6174
Size: 5026 1148
AflIII A'CryG_T
Cuts at: 0 1767 3926 5219 5729 6174
Size: 1767 2159 1293 510 445
Fragments arranged by size:
2159 1767 1293 510 445
AgeI A'CCGG_T
Cuts at: 0 3401 6174
Size: 3401 2773
AhdI GACnn_n'nnGTC
Cuts at: 0 204 1281 6174
Size: 204 1077 4893
Fragments arranged by size:
4893 1077 204
AleI CACnn'nnGTG
Cuts at: 0 3118 4159 6174
Size: 3118 1041 2015
Fragments arranged by size:
3118 2015 1041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2131 3169 4933 6174
Size: 2131 1038 1764 1241
Fragments arranged by size:
2131 1764 1241 1038
AlwNI CAG_nnn'CTG
Cuts at: 0 2199 2724 2754 4340 4524 4734 4893 5511
Size: 2199 525 30 1586 184 210 159 618
Cuts at: 5511 5724 6174
Size: 213 450
Fragments arranged by size:
2199 1586 618 525 450 213 210 184
159 30
ApaI G_GGCC'C
Cuts at: 0 2253 6174
Size: 2253 3921
ApaLI G'TGCA_C
Cuts at: 0 2106 6174
Size: 2106 4068
ApoI r'AATT_y
Cuts at: 0 72 829 1018 2896 4174 5347 5788 6174
Size: 72 757 189 1878 1278 1173 441 386
Fragments arranged by size:
1878 1278 1173 757 441 386 189 72
AvaI C'yCGr_G
Cuts at: 0 763 2824 3390 5060 5759 6174
Size: 763 2061 566 1670 699 415
Fragments arranged by size:
2061 1670 763 699 566 415
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5491 5524 6174
Size: 5491 33 650
Fragments arranged by size:
5491 650 33
BamHI G'GATC_C
Cuts at: 0 5686 6174
Size: 5686 488
BanI G'GyrC_C
Cuts at: 0 368 3120 3295 3394 3775 3991 6009 6174
Size: 368 2752 175 99 381 216 2018 165
Fragments arranged by size:
2752 2018 381 368 216 175 165 99
BanII G_rGCy'C
Cuts at: 0 221 2253 2543 2780 3676 6174
Size: 221 2032 290 237 896 2498
Fragments arranged by size:
2498 2032 896 290 237 221
BbeI G_GCGC'C
Cuts at: 0 372 6174
Size: 372 5802
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 571 637 893 2034 2167 4220 5970
Size: 90 481 66 256 1141 133 2053 1750
Cuts at: 5970 6174
Size: 204
Fragments arranged by size:
2053 1750 1141 481 256 204 133 90
66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2087 2100 2121 2134 6174
Size: 2087 13 21 13 4040
Fragments arranged by size:
4040 2087 21 13 13
BciVI GTATCCnnnnn_n'
Cuts at: 0 1785 4958 6174
Size: 1785 3173 1216
Fragments arranged by size:
3173 1785 1216
BclI T'GATC_A
Cuts at: 0 4061 5837 6174
Size: 4061 1776 337
Fragments arranged by size:
4061 1776 337
BfrBI ATG'CAT
Cuts at: 0 3957 6174
Size: 3957 2217
BglI GCCn_nnn'nGGC
Cuts at: 0 377 5123 6174
Size: 377 4746 1051
Fragments arranged by size:
4746 1051 377
BglII A'GATC_T
Cuts at: 0 1069 6174
Size: 1069 5105
BlpI GC'TnA_GC
Cuts at: 0 1876 6174
Size: 1876 4298
Bme1580I G_kGCm'C
Cuts at: 0 1041 2110 2253 3125 3778 3994 4853 6012
Size: 1041 1069 143 872 653 216 859 1159
Cuts at: 6012 6174
Size: 162
Fragments arranged by size:
1159 1069 1041 872 859 653 216 162
143
BmgBI CAC'GTC
Cuts at: 0 942 5250 6174
Size: 942 4308 924
Fragments arranged by size:
4308 942 924
BmrI ACTGGGnnnn_n'
Cuts at: 0 3128 4324 6174
Size: 3128 1196 1850
Fragments arranged by size:
3128 1850 1196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4951 6174
Size: 4951 1223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1670 2008 2221 2653 2698 2809 3183 4756
Size: 1670 338 213 432 45 111 374 1573
Cuts at: 4756 5464 5488 5650 6153 6174
Size: 708 24 162 503 21
Fragments arranged by size:
1670 1573 708 503 432 374 338 213
162 111 45 24 21
Bpu10I CC'TnA_GC
Cuts at: 0 4903 6174
Size: 4903 1271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1523 3580 3625 3754 4921 4930 5563
Size: 496 1027 2057 45 129 1167 9 633
Cuts at: 5563 6174
Size: 611
Fragments arranged by size:
2057 1167 1027 633 611 496 129 45
9
BsaAI yAC'GTr
Cuts at: 0 6026 6174
Size: 6026 148
BsaBI GATnn'nnATC
Cuts at: 0 3838 5143 6174
Size: 3838 1305 1031
Fragments arranged by size:
3838 1305 1031
BsaHI Gr'CG_yC
Cuts at: 0 205 369 2093 3005 5933 6174
Size: 205 164 1724 912 2928 241
Fragments arranged by size:
2928 1724 912 241 205 164
BsaWI w'CCGG_w
Cuts at: 0 101 2861 3068 3401 5678 6174
Size: 101 2760 207 333 2277 496
Fragments arranged by size:
2760 2277 496 333 207 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 274 304 1487 1517 2785 2815 3181 3211
Size: 274 30 1183 30 1268 30 366 30
Cuts at: 3211 3232 3262 4729 4759 5923 5953 6174
Size: 21 30 1467 30 1164 30 221
Fragments arranged by size:
1467 1268 1183 1164 366 274 221 30
30 30 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1872 2358 2361 2806 2944 2983 2986
Size: 199 1673 486 3 445 138 39 3
Cuts at: 2986 3106 3186 3666 3775 4123 5725 5728 5742
Size: 120 80 480 109 348 1602 3 14
Cuts at: 5742 6174
Size: 432
Fragments arranged by size:
1673 1602 486 480 445 432 348 199
138 120 109 80 39 14 3 3
3
BseYI C'CCAG_C
Cuts at: 0 2011 2154 2549 3028 3235 4517 5164 6174
Size: 2011 143 395 479 207 1282 647 1010
Fragments arranged by size:
2011 1282 1010 647 479 395 207 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 2823 3190 4069 4893 5515 6174
Size: 101 2722 367 879 824 622 659
Fragments arranged by size:
2722 879 824 659 622 367 101
BsiEI CG_ry'CG
Cuts at: 0 4505 6174
Size: 4505 1669
BsiHKAI G_wGCw'C
Cuts at: 0 142 221 2110 2543 2780 3038 6174
Size: 142 79 1889 433 237 258 3136
Fragments arranged by size:
3136 1889 433 258 237 142 79
BsmI GAATG_Cn'
Cuts at: 0 1477 1857 2332 3987 6174
Size: 1477 380 475 1655 2187
Fragments arranged by size:
2187 1655 1477 475 380
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5943 6174
Size: 5943 231
Bsp1286I G_dGCh'C
Cuts at: 0 142 221 1041 2110 2253 2543 2780 3038
Size: 142 79 820 1069 143 290 237 258
Cuts at: 3038 3125 3676 3778 3994 4853 6012 6174
Size: 87 551 102 216 859 1159 162
Fragments arranged by size:
1159 1069 859 820 551 290 258 237
216 162 143 142 102 87 79
BspEI T'CCGG_A
Cuts at: 0 101 2861 3068 5678 6174
Size: 101 2760 207 2610 496
Fragments arranged by size:
2760 2610 496 207 101
BspHI T'CATG_A
Cuts at: 0 6095 6174
Size: 6095 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 472 2646 2705 4802 5345 6174
Size: 472 2174 59 2097 543 829
Fragments arranged by size:
2174 2097 829 543 472 59
BsrBI CCG'CTC
Cuts at: 0 2763 2874 3623 4629 6174
Size: 2763 111 749 1006 1545
Fragments arranged by size:
2763 1545 1006 749 111
BsrDI GCAATG_nn'
Cuts at: 0 630 941 1275 6174
Size: 630 311 334 4899
Fragments arranged by size:
4899 630 334 311
BsrFI r'CCGG_y
Cuts at: 0 365 3401 3491 4424 6174
Size: 365 3036 90 933 1750
Fragments arranged by size:
3036 1750 933 365 90
BsrGI T'GTAC_A
Cuts at: 0 638 3506 3545 4877 6174
Size: 638 2868 39 1332 1297
Fragments arranged by size:
2868 1332 1297 638 39
BssSI C'ACGA_G
Cuts at: 0 2109 6174
Size: 2109 4065
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1929 2754 4794 6174
Size: 1929 825 2040 1380
Fragments arranged by size:
2040 1929 1380 825
BstBI TT'CG_AA
Cuts at: 0 2990 6174
Size: 2990 3184
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2910 3235 5829 6075 6174
Size: 2910 325 2594 246 99
Fragments arranged by size:
2910 2594 325 246 99
BstYI r'GATC_y
Cuts at: 0 1069 1151 2666 3944 4889 5686 6174
Size: 1069 82 1515 1278 945 797 488
Fragments arranged by size:
1515 1278 1069 945 797 488 82
BstZ17I GTA'TAC
Cuts at: 0 997 6174
Size: 997 5177
Bsu36I CC'TnA_GG
Cuts at: 0 1138 2087 5665 5903 6174
Size: 1138 949 3578 238 271
Fragments arranged by size:
3578 1138 949 271 238
BtgI C'CryG_G
Cuts at: 0 222 350 1205 2237 3932 5105 5118 5201
Size: 222 128 855 1032 1695 1173 13 83
Cuts at: 5201 5341 5448 5690 5816 6174
Size: 140 107 242 126 358
Fragments arranged by size:
1695 1173 1032 855 358 242 222 140
128 126 107 83 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 309 390 3092 6174
Size: 309 81 2702 3082
Fragments arranged by size:
3082 2702 309 81
BtsI GCAGTG_nn'
Cuts at: 0 793 1025 1852 4086 4869 6174
Size: 793 232 827 2234 783 1305
Fragments arranged by size:
2234 1305 827 793 783 232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5874 5909 6174
Size: 5874 35 265
Fragments arranged by size:
5874 265 35
DraIII CAC_nnn'GTG
Cuts at: 0 4460 6174
Size: 4460 1714
DrdI GACnn_nn'nnGTC
Cuts at: 0 2857 6174
Size: 2857 3317
EaeI y'GGCC_r
Cuts at: 0 48 1202 2234 3039 3848 4070 5198 5244
Size: 48 1154 1032 805 809 222 1128 46
Cuts at: 5244 5451 5819 6086 6174
Size: 207 368 267 88
Fragments arranged by size:
1154 1128 1032 809 805 368 267 222
207 88 48 46
EarI CTCTTCn'nnn_
Cuts at: 0 1142 2210 2468 2523 2593 2657 3090 3440
Size: 1142 1068 258 55 70 64 433 350
Cuts at: 3440 5014 5549 5629 6174
Size: 1574 535 80 545
Fragments arranged by size:
1574 1142 1068 545 535 433 350 258
80 70 64 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3712 5655 5685 6138 6174
Size: 3712 1943 30 453 36
Fragments arranged by size:
3712 1943 453 36 30
EcoICRI GAG'CTC
Cuts at: 0 219 2541 2778 6174
Size: 219 2322 237 3396
Fragments arranged by size:
3396 2322 237 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1357 1670 2008 2221 2653 2698 2809 2934
Size: 1357 313 338 213 432 45 111 125
Cuts at: 2934 3071 3183 4261 4678 4756 5464 5488 5650
Size: 137 112 1078 417 78 708 24 162
Cuts at: 5650 6153 6174
Size: 503 21
Fragments arranged by size:
1357 1078 708 503 432 417 338 313
213 162 137 125 112 111 78 45
24 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2365 6174
Size: 2365 3809
EcoO109I rG'GnC_Cy
Cuts at: 0 2292 2694 3096 3377 3636 4319 4497 5334
Size: 2292 402 402 281 259 683 178 837
Cuts at: 5334 5660 6174
Size: 326 514
Fragments arranged by size:
2292 837 683 514 402 402 326 281
259 178
EcoRI G'AATT_C
Cuts at: 0 4174 6174
Size: 4174 2000
EcoRV GAT'ATC
Cuts at: 0 4536 6174
Size: 4536 1638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2958 6174
Size: 2958 3216
HaeII r_GCGC'y
Cuts at: 0 372 441 3711 6132 6174
Size: 372 69 3270 2421 42
Fragments arranged by size:
3270 2421 372 69 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 553 585 895 927 1136 1168
Size: 190 32 331 32 310 32 209 32
Cuts at: 1168 1267 1299 2058 2090 2658 2690 3232 3264
Size: 99 32 759 32 568 32 542 32
Cuts at: 3264 4729 4761 5678 5710 5753 5785 5923 5955
Size: 1465 32 917 32 43 32 138 32
Cuts at: 5955 6174
Size: 219
Fragments arranged by size:
1465 917 759 568 542 331 310 219
209 190 138 99 43 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 535 1623 5253 5600 6174
Size: 535 1088 3630 347 574
Fragments arranged by size:
3630 1088 574 535 347
HindIII A'AGCT_T
Cuts at: 0 614 6174
Size: 614 5560
HpaI GTT'AAC
Cuts at: 0 1623 6174
Size: 1623 4551
KasI G'GCGC_C
Cuts at: 0 368 6174
Size: 368 5806
MfeI C'AATT_G
Cuts at: 0 2428 6174
Size: 2428 3746
MluI A'CGCG_T
Cuts at: 0 3926 6174
Size: 3926 2248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2162 3353 6174
Size: 2162 1191 2821
Fragments arranged by size:
2821 2162 1191
MscI TGG'CCA
Cuts at: 0 50 1204 5200 5453 5821 6088 6174
Size: 50 1154 3996 253 368 267 86
Fragments arranged by size:
3996 1154 368 267 253 86 50
MslI CAynn'nnrTG
Cuts at: 0 1598 3118 3844 4159 6112 6118 6174
Size: 1598 1520 726 315 1953 6 56
Fragments arranged by size:
1953 1598 1520 726 315 56 6
MspA1I CmG'CkG
Cuts at: 0 255 425 678 1968 2154 3203 3365 3512
Size: 255 170 253 1290 186 1049 162 147
Cuts at: 3512 3584 4524 4883 5164 6174
Size: 72 940 359 281 1010
Fragments arranged by size:
1290 1049 1010 940 359 281 255 253
186 170 162 147 72
NaeI GCC'GGC
Cuts at: 0 367 6174
Size: 367 5807
NarI GG'CG_CC
Cuts at: 0 369 6174
Size: 369 5805
NcoI C'CATG_G
Cuts at: 0 222 350 1205 2237 5118 5201 5341 5448
Size: 222 128 855 1032 2881 83 140 107
Cuts at: 5448 5816 6174
Size: 368 358
Fragments arranged by size:
2881 1032 855 368 358 222 140 128
107 83
NgoMIV G'CCGG_C
Cuts at: 0 365 6174
Size: 365 5809
NsiI A_TGCA'T
Cuts at: 0 3959 6174
Size: 3959 2215
NspI r_CATG'y
Cuts at: 0 1585 1771 1923 2927 3225 4901 5223 5733
Size: 1585 186 152 1004 298 1676 322 510
Cuts at: 5733 6174
Size: 441
Fragments arranged by size:
1676 1585 1004 510 441 322 298 186
152
PciI A'CATG_T
Cuts at: 0 1767 5219 5729 6174
Size: 1767 3452 510 445
Fragments arranged by size:
3452 1767 510 445
PflMI CCAn_nnn'nTGG
Cuts at: 0 2259 3899 4460 6174
Size: 2259 1640 561 1714
Fragments arranged by size:
2259 1714 1640 561
PfoI T'CCnGG_A
Cuts at: 0 3178 3688 3947 4980 5372 6174
Size: 3178 510 259 1033 392 802
Fragments arranged by size:
3178 1033 802 510 392 259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5981 6013 6174
Size: 5981 32 161
Fragments arranged by size:
5981 161 32
PpuMI rG'GwC_Cy
Cuts at: 0 2292 2694 3096 3377 4319 5334 5660 6174
Size: 2292 402 402 281 942 1015 326 514
Fragments arranged by size:
2292 1015 942 514 402 402 326 281
PshAI GACnn'nnGTC
Cuts at: 0 113 2167 4324 6174
Size: 113 2054 2157 1850
Fragments arranged by size:
2157 2054 1850 113
PspOMI G'GGCC_C
Cuts at: 0 2249 6174
Size: 2249 3925
PspXI vC'TCGA_Gb
Cuts at: 0 2824 5759 6174
Size: 2824 2935 415
Fragments arranged by size:
2935 2824 415
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4933 4965 6174
Size: 4933 32 1209
Fragments arranged by size:
4933 1209 32
PstI C_TGCA'G
Cuts at: 0 2123 2702 2750 2759 4139 4730 5516 5573
Size: 2123 579 48 9 1380 591 786 57
Cuts at: 5573 6174
Size: 601
Fragments arranged by size:
2123 1380 786 601 591 579 57 48
9
PvuI CG_AT'CG
Cuts at: 0 4505 6174
Size: 4505 1669
PvuII CAG'CTG
Cuts at: 0 678 1968 2154 3512 4524 4883 6174
Size: 678 1290 186 1358 1012 359 1291
Fragments arranged by size:
1358 1291 1290 1012 678 359 186
RsrII CG'GwC_CG
Cuts at: 0 2022 6174
Size: 2022 4152
SacI G_AGCT'C
Cuts at: 0 221 2543 2780 6174
Size: 221 2322 237 3394
Fragments arranged by size:
3394 2322 237 221
SalI G'TCGA_C
Cuts at: 0 5251 6174
Size: 5251 923
SanDI GG'GwC_CC
Cuts at: 0 4319 5660 6174
Size: 4319 1341 514
Fragments arranged by size:
4319 1341 514
SapI GCTCTTCn'nnn_
Cuts at: 0 2593 6174
Size: 2593 3581
ScaI AGT'ACT
Cuts at: 0 66 1381 6174
Size: 66 1315 4793
Fragments arranged by size:
4793 1315 66
SfcI C'TryA_G
Cuts at: 0 1826 2119 2698 2746 2755 4135 4726 4777
Size: 1826 293 579 48 9 1380 591 51
Cuts at: 4777 5512 5569 6132 6174
Size: 735 57 563 42
Fragments arranged by size:
1826 1380 735 591 579 563 293 57
51 48 42 9
SfoI GGC'GCC
Cuts at: 0 370 6174
Size: 370 5804
SmaI CCC'GGG
Cuts at: 0 765 6174
Size: 765 5409
SmlI C'TyrA_G
Cuts at: 0 511 1538 2824 3595 3640 3769 4936 4945
Size: 511 1027 1286 771 45 129 1167 9
Cuts at: 4945 5026 5578 5759 6174
Size: 81 552 181 415
Fragments arranged by size:
1286 1167 1027 771 552 511 415 181
129 81 45 9
SnaBI TAC'GTA
Cuts at: 0 6026 6174
Size: 6026 148
SphI G_CATG'C
Cuts at: 0 1585 2927 3225 6174
Size: 1585 1342 298 2949
Fragments arranged by size:
2949 1585 1342 298
SspI AAT'ATT
Cuts at: 0 702 6174
Size: 702 5472
StuI AGG'CCT
Cuts at: 0 5195 6174
Size: 5195 979
StyI C'CwwG_G
Cuts at: 0 222 350 1205 2165 2186 2237 3821 3995
Size: 222 128 855 960 21 51 1584 174
Cuts at: 3995 4493 5118 5201 5341 5448 5816 6174
Size: 498 625 83 140 107 368 358
Fragments arranged by size:
1584 960 855 625 498 368 358 222
174 140 128 107 83 51 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2749 4279 4531 4621 5908 6174
Size: 2749 1530 252 90 1287 266
Fragments arranged by size:
2749 1530 1287 266 252 90
TatI w'GTAC_w
Cuts at: 0 64 359 638 1379 3506 3545 3740 4257
Size: 64 295 279 741 2127 39 195 517
Cuts at: 4257 4817 4877 5825 6174
Size: 560 60 948 349
Fragments arranged by size:
2127 948 741 560 517 349 295 279
195 64 60 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2785 2817 3181 3213 6174
Size: 2785 32 364 32 2961
Fragments arranged by size:
2961 2785 364 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 5935 6174
Size: 5935 239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3058 5161 6075 6174
Size: 3058 2103 914 99
Fragments arranged by size:
3058 2103 914 99
XhoI C'TCGA_G
Cuts at: 0 2824 5759 6174
Size: 2824 2935 415
Fragments arranged by size:
2935 2824 415
XmaI C'CCGG_G
Cuts at: 0 763 6174
Size: 763 5411
XmnI GAAnn'nnTTC
Cuts at: 0 4369 4976 6174
Size: 4369 607 1198
Fragments arranged by size:
4369 1198 607
ZraI GAC'GTC
Cuts at: 0 2094 6174
Size: 2094 4080
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI ScaI SfcI SfoI SmaI SmlI SnaBI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AloI AscI AseI AsiSI AvrII BbvCI
BmtI BsaI BsiWI BssHII BstEII ClaI DraI EagI
FseI FspI FspAI KpnI NdeI NheI NotI NruI
PacI PasI PmeI PmlI PsiI SacII SbfI SexAI
SfiI SgfI SgrAI SpeI SrfI SwaI XbaI