Restriction map for
pF1KA0790
(Linear) (Six-base) MAPSORT of: KIAA0790.seq Check: 6502 from: 1 to: 3741
KIAA0790.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 3422 3741
Size: 3422 319
AccI GT'mk_AC
Cuts at: 0 2663 3741
Size: 2663 1078
AclI AA'CG_TT
Cuts at: 0 703 3741
Size: 703 3038
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 878 1044 2353 2618 3329 3389 3591
Size: 298 580 166 1309 265 711 60 202
Cuts at: 3591 3634 3741
Size: 43 107
Fragments arranged by size:
1309 711 580 298 265 202 166 107
60 43
AflIII A'CryG_T
Cuts at: 0 227 875 2381 3741
Size: 227 648 1506 1360
Fragments arranged by size:
1506 1360 648 227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 212 677 2672 3741
Size: 212 465 1995 1069
Fragments arranged by size:
1995 1069 465 212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2868 2900 3741
Size: 2868 32 841
Fragments arranged by size:
2868 841 32
AlwNI CAG_nnn'CTG
Cuts at: 0 276 2148 2643 2752 3730 3741
Size: 276 1872 495 109 978 11
Fragments arranged by size:
1872 978 495 276 109 11
ApaI G_GGCC'C
Cuts at: 0 2482 3019 3030 3235 3741
Size: 2482 537 11 205 506
Fragments arranged by size:
2482 537 506 205 11
ApaLI G'TGCA_C
Cuts at: 0 928 1681 3741
Size: 928 753 2060
Fragments arranged by size:
2060 928 753
ApoI r'AATT_y
Cuts at: 0 885 2936 3741
Size: 885 2051 805
Fragments arranged by size:
2051 885 805
AvaI C'yCGr_G
Cuts at: 0 24 43 49 61 1189 1431 2165 3193
Size: 24 19 6 12 1128 242 734 1028
Cuts at: 3193 3263 3409 3741
Size: 70 146 332
Fragments arranged by size:
1128 1028 734 332 242 146 70 24
19 12 6
BamHI G'GATC_C
Cuts at: 0 2113 3741
Size: 2113 1628
BanI G'GyrC_C
Cuts at: 0 2893 3141 3586 3741
Size: 2893 248 445 155
Fragments arranged by size:
2893 445 248 155
BanII G_rGCy'C
Cuts at: 0 44 50 62 68 92 1558 2258 2482
Size: 44 6 12 6 24 1466 700 224
Cuts at: 2482 2740 3019 3030 3191 3235 3741
Size: 258 279 11 161 44 506
Fragments arranged by size:
1466 700 506 279 258 224 161 44
44 24 12 11 6 6
BbeI G_GCGC'C
Cuts at: 0 3145 3741
Size: 3145 596
BbsI GAAGACnn'nnnn_
Cuts at: 0 419 524 1522 1850 2018 2617 3741
Size: 419 105 998 328 168 599 1124
Fragments arranged by size:
1124 998 599 419 328 168 105
BciVI GTATCCnnnnn_n'
Cuts at: 0 160 3741
Size: 160 3581
BclI T'GATC_A
Cuts at: 0 599 3741
Size: 599 3142
BfrBI ATG'CAT
Cuts at: 0 2671 3741
Size: 2671 1070
BglI GCCn_nnn'nGGC
Cuts at: 0 3177 3741
Size: 3177 564
BlpI GC'TnA_GC
Cuts at: 0 2546 2801 2915 3741
Size: 2546 255 114 826
Fragments arranged by size:
2546 826 255 114
Bme1580I G_kGCm'C
Cuts at: 0 932 1685 2482 2896 3019 3030 3235 3264
Size: 932 753 797 414 123 11 205 29
Cuts at: 3264 3741
Size: 477
Fragments arranged by size:
932 797 753 477 414 205 123 29
11
BmgBI CAC'GTC
Cuts at: 0 141 228 308 1001 1833 2384 2556 3741
Size: 141 87 80 693 832 551 172 1185
Fragments arranged by size:
1185 832 693 551 172 141 87 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 175 1694 2437 3014 3029 3741
Size: 175 1519 743 577 15 712
Fragments arranged by size:
1519 743 712 577 175 15
BmtI G_CTAG'C
Cuts at: 0 302 2228 3741
Size: 302 1926 1513
Fragments arranged by size:
1926 1513 302
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 1446 2074 2670 3741
Size: 138 1308 628 596 1071
Fragments arranged by size:
1308 1071 628 596 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 2023 2494 2665 2752 2831 2863 3193
Size: 996 1027 471 171 87 79 32 330
Cuts at: 3193 3741
Size: 548
Fragments arranged by size:
1027 996 548 471 330 171 87 79
32
Bpu10I CC'TnA_GC
Cuts at: 0 38 1198 3741
Size: 38 1160 2543
Fragments arranged by size:
2543 1160 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1489 1708 1714 3741
Size: 613 876 219 6 2027
Fragments arranged by size:
2027 876 613 219 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 1192 2461 3258 3741
Size: 1192 1269 797 483
Fragments arranged by size:
1269 1192 797 483
BsaAI yAC'GTr
Cuts at: 0 876 1827 3741
Size: 876 951 1914
Fragments arranged by size:
1914 951 876
BsaBI GATnn'nnATC
Cuts at: 0 148 3741
Size: 148 3593
BsaHI Gr'CG_yC
Cuts at: 0 1878 2836 3142 3419 3741
Size: 1878 958 306 277 322
Fragments arranged by size:
1878 958 322 306 277
BsaWI w'CCGG_w
Cuts at: 0 84 3741
Size: 84 3657
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 506 536 862 892 987 1017 1286 1316
Size: 506 30 326 30 95 30 269 30
Cuts at: 1316 3319 3349 3741
Size: 2003 30 392
Fragments arranged by size:
2003 506 392 326 269 95 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 574 772 883 1864 2020 2362 3343
Size: 259 315 198 111 981 156 342 981
Cuts at: 3343 3685 3741
Size: 342 56
Fragments arranged by size:
981 981 342 342 315 259 198 156
111 56
BseYI C'CCAG_C
Cuts at: 0 644 850 1898 2257 2358 2636 3108 3228
Size: 644 206 1048 359 101 278 472 120
Cuts at: 3228 3741
Size: 513
Fragments arranged by size:
1048 644 513 472 359 278 206 120
101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 3286 3400 3741
Size: 1237 2049 114 341
Fragments arranged by size:
2049 1237 341 114
BsiEI CG_ry'CG
Cuts at: 0 2618 3741
Size: 2618 1123
BsiHKAI G_wGCw'C
Cuts at: 0 875 932 1558 1582 1685 1838 1949 3191
Size: 875 57 626 24 103 153 111 1242
Cuts at: 3191 3218 3741
Size: 27 523
Fragments arranged by size:
1242 875 626 523 153 111 103 57
27 24
BsmI GAATG_Cn'
Cuts at: 0 1451 2930 3741
Size: 1451 1479 811
Fragments arranged by size:
1479 1451 811
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1007 1369 3525 3741
Size: 1007 362 2156 216
Fragments arranged by size:
2156 1007 362 216
Bsp1286I G_dGCh'C
Cuts at: 0 44 50 62 68 92 875 932 1558
Size: 44 6 12 6 24 783 57 626
Cuts at: 1558 1582 1685 1838 1949 2258 2482 2740 2896
Size: 24 103 153 111 309 224 258 156
Cuts at: 2896 3019 3030 3191 3218 3235 3264 3741
Size: 123 11 161 27 17 29 477
Fragments arranged by size:
783 626 477 309 258 224 161 156
153 123 111 103 57 44 29 27
24 24 17 12 11 6 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1222 3741
Size: 1222 2519
BsrBI CCG'CTC
Cuts at: 0 564 1157 1565 3414 3741
Size: 564 593 408 1849 327
Fragments arranged by size:
1849 593 564 408 327
BsrDI GCAATG_nn'
Cuts at: 0 2302 2671 3741
Size: 2302 369 1070
Fragments arranged by size:
2302 1070 369
BsrFI r'CCGG_y
Cuts at: 0 650 891 3583 3658 3741
Size: 650 241 2692 75 83
Fragments arranged by size:
2692 650 241 83 75
BsrGI T'GTAC_A
Cuts at: 0 1304 3741
Size: 1304 2437
BssSI C'ACGA_G
Cuts at: 0 2863 3741
Size: 2863 878
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2556 2925 3741
Size: 2556 369 816
Fragments arranged by size:
2556 816 369
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1020 1104 1911 3741
Size: 1020 84 807 1830
Fragments arranged by size:
1830 1020 807 84
BstYI r'GATC_y
Cuts at: 0 1917 2113 3273 3440 3741
Size: 1917 196 1160 167 301
Fragments arranged by size:
1917 1160 301 196 167
Bsu36I CC'TnA_GG
Cuts at: 0 3731 3741
Size: 3731 10
BtgI C'CryG_G
Cuts at: 0 1203 1769 3011 3599 3741
Size: 1203 566 1242 588 142
Fragments arranged by size:
1242 1203 588 566 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 569 3741
Size: 569 3172
BtsI GCAGTG_nn'
Cuts at: 0 2075 2196 3741
Size: 2075 121 1545
Fragments arranged by size:
2075 1545 121
ClaI AT'CG_AT
Cuts at: 0 176 1748 3317 3741
Size: 176 1572 1569 424
Fragments arranged by size:
1572 1569 424 176
DraIII CAC_nnn'GTG
Cuts at: 0 2407 3222 3741
Size: 2407 815 519
Fragments arranged by size:
2407 815 519
DrdI GACnn_nn'nnGTC
Cuts at: 0 759 2659 3741
Size: 759 1900 1082
Fragments arranged by size:
1900 1082 759
EaeI y'GGCC_r
Cuts at: 0 682 1038 3351 3741
Size: 682 356 2313 390
Fragments arranged by size:
2313 682 390 356
EarI CTCTTCn'nnn_
Cuts at: 0 329 398 1634 2628 3715 3741
Size: 329 69 1236 994 1087 26
Fragments arranged by size:
1236 1087 994 329 69 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 900 1451 3741
Size: 900 551 2290
Fragments arranged by size:
2290 900 551
EcoICRI GAG'CTC
Cuts at: 0 1556 3189 3741
Size: 1556 1633 552
Fragments arranged by size:
1633 1556 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 878 996 1044 2023 2353 2494 2618
Size: 298 580 118 48 979 330 141 124
Cuts at: 2618 2665 2752 2831 2863 3193 3329 3389 3591
Size: 47 87 79 32 330 136 60 202
Cuts at: 3591 3634 3741
Size: 43 107
Fragments arranged by size:
979 580 330 330 298 202 141 136
124 118 107 87 79 60 48 47
43 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1165 1652 3131 3652 3741
Size: 1165 487 1479 521 89
Fragments arranged by size:
1479 1165 521 487 89
EcoO109I rG'GnC_Cy
Cuts at: 0 273 1080 1193 1775 2037 2145 2478 2479
Size: 273 807 113 582 262 108 333 1
Cuts at: 2479 3016 3026 3027 3727 3741
Size: 537 10 1 700 14
Fragments arranged by size:
807 700 582 537 333 273 262 113
108 14 10 1 1
EcoRI G'AATT_C
Cuts at: 0 885 3741
Size: 885 2856
EcoRV GAT'ATC
Cuts at: 0 1743 3741
Size: 1743 1998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 358 3741
Size: 358 3383
HaeII r_GCGC'y
Cuts at: 0 359 3145 3741
Size: 359 2786 596
Fragments arranged by size:
2786 596 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 892 924 973 1005 1702 1730
Size: 700 32 160 32 49 32 697 28
Cuts at: 1730 1734 1762 3741
Size: 4 28 1979
Fragments arranged by size:
1979 700 697 160 49 32 32 32
28 28 4
HincII GTy'rAC
Cuts at: 0 365 2136 2664 3741
Size: 365 1771 528 1077
Fragments arranged by size:
1771 1077 528 365
KasI G'GCGC_C
Cuts at: 0 3141 3741
Size: 3141 600
MfeI C'AATT_G
Cuts at: 0 605 3741
Size: 605 3136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1601 1861 3741
Size: 248 1353 260 1880
Fragments arranged by size:
1880 1353 260 248
MscI TGG'CCA
Cuts at: 0 684 3741
Size: 684 3057
MslI CAynn'nnrTG
Cuts at: 0 1825 3741
Size: 1825 1916
MspA1I CmG'CkG
Cuts at: 0 19 318 3356 3601 3741
Size: 19 299 3038 245 140
Fragments arranged by size:
3038 299 245 140 19
NaeI GCC'GGC
Cuts at: 0 3585 3660 3741
Size: 3585 75 81
Fragments arranged by size:
3585 81 75
NarI GG'CG_CC
Cuts at: 0 3142 3741
Size: 3142 599
NcoI C'CATG_G
Cuts at: 0 1769 3011 3741
Size: 1769 1242 730
Fragments arranged by size:
1769 1242 730
NgoMIV G'CCGG_C
Cuts at: 0 3583 3658 3741
Size: 3583 75 83
Fragments arranged by size:
3583 83 75
NheI G'CTAG_C
Cuts at: 0 298 2224 3741
Size: 298 1926 1517
Fragments arranged by size:
1926 1517 298
NsiI A_TGCA'T
Cuts at: 0 2673 3741
Size: 2673 1068
NspI r_CATG'y
Cuts at: 0 1953 3741
Size: 1953 1788
PasI CC'CwG_GG
Cuts at: 0 2889 3741
Size: 2889 852
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 2490 2517 2643 2889 3490 3741
Size: 276 2214 27 126 246 601 251
Fragments arranged by size:
2214 601 276 251 246 126 27
PfoI T'CCnGG_A
Cuts at: 0 152 896 1922 2808 3209 3741
Size: 152 744 1026 886 401 532
Fragments arranged by size:
1026 886 744 532 401 152
PmlI CAC'GTG
Cuts at: 0 876 3741
Size: 876 2865
PpuMI rG'GwC_Cy
Cuts at: 0 273 1193 1775 2037 3741
Size: 273 920 582 262 1704
Fragments arranged by size:
1704 920 582 273 262
PspOMI G'GGCC_C
Cuts at: 0 2478 3015 3026 3231 3741
Size: 2478 537 11 205 510
Fragments arranged by size:
2478 537 510 205 11
PstI C_TGCA'G
Cuts at: 0 1219 3702 3741
Size: 1219 2483 39
Fragments arranged by size:
2483 1219 39
PvuII CAG'CTG
Cuts at: 0 19 318 3741
Size: 19 299 3423
Fragments arranged by size:
3423 299 19
RsrII CG'GwC_CG
Cuts at: 0 908 3741
Size: 908 2833
SacI G_AGCT'C
Cuts at: 0 1558 3191 3741
Size: 1558 1633 550
Fragments arranged by size:
1633 1558 550
SacII CC_GC'GG
Cuts at: 0 3602 3741
Size: 3602 139
SalI G'TCGA_C
Cuts at: 0 2662 3741
Size: 2662 1079
SanDI GG'GwC_CC
Cuts at: 0 1193 2037 3741
Size: 1193 844 1704
Fragments arranged by size:
1704 1193 844
SapI GCTCTTCn'nnn_
Cuts at: 0 1634 3741
Size: 1634 2107
SexAI A'CCwGG_T
Cuts at: 0 2403 3741
Size: 2403 1338
SfcI C'TryA_G
Cuts at: 0 351 422 1215 1520 3698 3741
Size: 351 71 793 305 2178 43
Fragments arranged by size:
2178 793 351 305 71 43
SfoI GGC'GCC
Cuts at: 0 3143 3741
Size: 3143 598
SmaI CCC'GGG
Cuts at: 0 26 3265 3741
Size: 26 3239 476
Fragments arranged by size:
3239 476 26
SmlI C'TyrA_G
Cuts at: 0 628 1504 1723 1729 3741
Size: 628 876 219 6 2012
Fragments arranged by size:
2012 876 628 219 6
StuI AGG'CCT
Cuts at: 0 3126 3741
Size: 3126 615
StyI C'CwwG_G
Cuts at: 0 1769 3011 3741
Size: 1769 1242 730
Fragments arranged by size:
1769 1242 730
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 1782 2350 3518 3556 3741
Size: 151 1631 568 1168 38 185
Fragments arranged by size:
1631 1168 568 185 151 38
TatI w'GTAC_w
Cuts at: 0 1304 3741
Size: 1304 2437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 987 1019 1286 1318 1686 1718 3741
Size: 987 32 267 32 368 32 2023
Fragments arranged by size:
2023 987 368 267 32 32 32
XbaI T'CTAG_A
Cuts at: 0 3276 3741
Size: 3276 465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2149 3741
Size: 2149 1592
XmaI C'CCGG_G
Cuts at: 0 24 3263 3741
Size: 24 3239 478
Fragments arranged by size:
3239 478 24
XmnI GAAnn'nnTTC
Cuts at: 0 1529 3741
Size: 1529 2212
ZraI GAC'GTC
Cuts at: 0 3420 3741
Size: 3420 321
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII RsrII SacI
SacII SalI SanDI SapI SexAI SfcI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AhdI AleI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BglII BsiWI
BspEI BspHI BssHII BstBI BstEII BstZ17I CspCI DraI
EagI FseI FspI FspAI HindIII HpaI KpnI MluI
NdeI NotI NruI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XhoI