Restriction map for
pF1KA0777
(Linear) (Six-base) MAPSORT of: KIAA0777.seq Check: 8163 from: 1 to: 3300
KIAA0777.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1967 3300
Size: 1967 1333
AccI GT'mk_AC
Cuts at: 0 272 2669 3300
Size: 272 2397 631
Fragments arranged by size:
2397 631 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 741 1369 3300
Size: 652 89 628 1931
Fragments arranged by size:
1931 652 628 89
AfeI AGC'GCT
Cuts at: 0 2330 3300
Size: 2330 970
AflIII A'CryG_T
Cuts at: 0 347 513 1773 3300
Size: 347 166 1260 1527
Fragments arranged by size:
1527 1260 347 166
AhdI GACnn_n'nnGTC
Cuts at: 0 1590 2508 3300
Size: 1590 918 792
Fragments arranged by size:
1590 918 792
AleI CACnn'nnGTG
Cuts at: 0 2860 3300
Size: 2860 440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 3300
Size: 88 3212
AlwNI CAG_nnn'CTG
Cuts at: 0 129 1967 2468 3144 3300
Size: 129 1838 501 676 156
Fragments arranged by size:
1838 676 501 156 129
ApaI G_GGCC'C
Cuts at: 0 230 3300
Size: 230 3070
ApaLI G'TGCA_C
Cuts at: 0 322 2492 2555 3300
Size: 322 2170 63 745
Fragments arranged by size:
2170 745 322 63
ApoI r'AATT_y
Cuts at: 0 311 610 1534 1572 2130 3259 3300
Size: 311 299 924 38 558 1129 41
Fragments arranged by size:
1129 924 558 311 299 41 38
AvaI C'yCGr_G
Cuts at: 0 1034 1338 1505 1694 1766 2305 3300
Size: 1034 304 167 189 72 539 995
Fragments arranged by size:
1034 995 539 304 189 167 72
BamHI G'GATC_C
Cuts at: 0 579 1909 3300
Size: 579 1330 1391
Fragments arranged by size:
1391 1330 579
BanI G'GyrC_C
Cuts at: 0 136 1500 1515 1728 1979 3300
Size: 136 1364 15 213 251 1321
Fragments arranged by size:
1364 1321 251 213 136 15
BanII G_rGCy'C
Cuts at: 0 230 971 1219 2054 2344 3076 3185 3300
Size: 230 741 248 835 290 732 109 115
Fragments arranged by size:
835 741 732 290 248 230 115 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 3300
Size: 548 2752
BbvCI CC'TCA_GC
Cuts at: 0 2332 3011 3300
Size: 2332 679 289
Fragments arranged by size:
2332 679 289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 72 106 3300
Size: 72 34 3194
Fragments arranged by size:
3194 72 34
BclI T'GATC_A
Cuts at: 0 1872 2688 2913 3300
Size: 1872 816 225 387
Fragments arranged by size:
1872 816 387 225
BglI GCCn_nnn'nGGC
Cuts at: 0 1706 2058 3300
Size: 1706 352 1242
Fragments arranged by size:
1706 1242 352
Bme1580I G_kGCm'C
Cuts at: 0 230 326 1505 1520 1733 1984 2496 2559
Size: 230 96 1179 15 213 251 512 63
Cuts at: 2559 3300
Size: 741
Fragments arranged by size:
1179 741 512 251 230 213 96 63
15
BmgBI CAC'GTC
Cuts at: 0 2448 3300
Size: 2448 852
BmrI ACTGGGnnnn_n'
Cuts at: 0 493 585 3300
Size: 493 92 2715
Fragments arranged by size:
2715 493 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 865 1254 2331 3300
Size: 865 389 1077 969
Fragments arranged by size:
1077 969 865 389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 509 569 969 1122 1497 1684 2564
Size: 45 464 60 400 153 375 187 880
Cuts at: 2564 3199 3300
Size: 635 101
Fragments arranged by size:
880 635 464 400 375 187 153 101
60 45
Bpu10I CC'TnA_GC
Cuts at: 0 2332 3011 3300
Size: 2332 679 289
Fragments arranged by size:
2332 679 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3090 3231 3300
Size: 3090 141 69
Fragments arranged by size:
3090 141 69
BsaAI yAC'GTr
Cuts at: 0 1776 2748 3300
Size: 1776 972 552
Fragments arranged by size:
1776 972 552
BsaWI w'CCGG_w
Cuts at: 0 241 2117 3300
Size: 241 1876 1183
Fragments arranged by size:
1876 1183 241
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1110 1140 2651 2681 2876 2906 3300
Size: 1110 30 1511 30 195 30 394
Fragments arranged by size:
1511 1110 394 195 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1246 1510 2062 2349 2352 3300
Size: 1246 264 552 287 3 948
Fragments arranged by size:
1246 948 552 287 264 3
BseYI C'CCAG_C
Cuts at: 0 45 113 2804 3300
Size: 45 68 2691 496
Fragments arranged by size:
2691 496 68 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2254 2332 3300
Size: 2254 78 968
Fragments arranged by size:
2254 968 78
BsiEI CG_ry'CG
Cuts at: 0 276 3300
Size: 276 3024
BsiHKAI G_wGCw'C
Cuts at: 0 326 405 1489 1773 2496 2559 3076 3185
Size: 326 79 1084 284 723 63 517 109
Cuts at: 3185 3300
Size: 115
Fragments arranged by size:
1084 723 517 326 284 115 109 79
63
BsmI GAATG_Cn'
Cuts at: 0 2426 3300
Size: 2426 874
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1128 3300
Size: 1128 2172
Bsp1286I G_dGCh'C
Cuts at: 0 230 326 405 971 1219 1489 1505 1520
Size: 230 96 79 566 248 270 16 15
Cuts at: 1520 1733 1773 1984 2054 2344 2496 2559 3076
Size: 213 40 211 70 290 152 63 517
Cuts at: 3076 3185 3300
Size: 109 115
Fragments arranged by size:
566 517 290 270 248 230 213 211
152 115 109 96 79 70 63 40
16 15
BspEI T'CCGG_A
Cuts at: 0 241 2117 3300
Size: 241 1876 1183
Fragments arranged by size:
1876 1183 241
BspHI T'CATG_A
Cuts at: 0 3105 3300
Size: 3105 195
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 367 1472 1967 3300
Size: 367 1105 495 1333
Fragments arranged by size:
1333 1105 495 367
BsrBI CCG'CTC
Cuts at: 0 1693 3300
Size: 1693 1607
BsrFI r'CCGG_y
Cuts at: 0 275 1700 3300
Size: 275 1425 1600
Fragments arranged by size:
1600 1425 275
BsrGI T'GTAC_A
Cuts at: 0 383 1355 3300
Size: 383 972 1945
Fragments arranged by size:
1945 972 383
BssHII G'CGCG_C
Cuts at: 0 1470 3300
Size: 1470 1830
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2782 3300
Size: 2782 518
BstBI TT'CG_AA
Cuts at: 0 1538 3300
Size: 1538 1762
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2485 3300
Size: 2485 815
BstYI r'GATC_y
Cuts at: 0 579 1909 3300
Size: 579 1330 1391
Fragments arranged by size:
1391 1330 579
Bsu36I CC'TnA_GG
Cuts at: 0 2677 3300
Size: 2677 623
BtgI C'CryG_G
Cuts at: 0 230 796 1256 1490 2484 2715 3300
Size: 230 566 460 234 994 231 585
Fragments arranged by size:
994 585 566 460 234 231 230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1769 3300
Size: 1769 1531
BtsI GCAGTG_nn'
Cuts at: 0 791 1863 2365 2436 3300
Size: 791 1072 502 71 864
Fragments arranged by size:
1072 864 791 502 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 785 820 3300
Size: 785 35 2480
Fragments arranged by size:
2480 785 35
DraIII CAC_nnn'GTG
Cuts at: 0 321 3300
Size: 321 2979
DrdI GACnn_nn'nnGTC
Cuts at: 0 1195 3300
Size: 1195 2105
EaeI y'GGCC_r
Cuts at: 0 746 3300
Size: 746 2554
EarI CTCTTCn'nnn_
Cuts at: 0 173 178 766 897 2565 3300
Size: 173 5 588 131 1668 735
Fragments arranged by size:
1668 735 588 173 131 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2786 3300
Size: 2786 514
EcoICRI GAG'CTC
Cuts at: 0 3074 3183 3300
Size: 3074 109 117
Fragments arranged by size:
3074 117 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 509 569 652 741 969 1122 1369
Size: 45 464 60 83 89 228 153 247
Cuts at: 1369 1497 1684 2564 3199 3300
Size: 128 187 880 635 101
Fragments arranged by size:
880 635 464 247 228 187 153 128
101 89 83 60 45
EcoO109I rG'GnC_Cy
Cuts at: 0 1268 1386 1461 3242 3300
Size: 1268 118 75 1781 58
Fragments arranged by size:
1781 1268 118 75 58
EcoRI G'AATT_C
Cuts at: 0 1534 3300
Size: 1534 1766
FspI TGC'GCA
Cuts at: 0 122 3300
Size: 122 3178
HaeII r_GCGC'y
Cuts at: 0 2332 3300
Size: 2332 968
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 179 211 478 510 1295 1327 1576
Size: 26 153 32 267 32 785 32 249
Cuts at: 1576 1608 1831 1863 2494 2526 2651 2683 2714
Size: 32 223 32 631 32 125 32 31
Cuts at: 2714 2746 2945 2977 3300
Size: 32 199 32 323
Fragments arranged by size:
785 631 323 267 249 223 199 153
125 32 32 32 32 32 32 32
32 32 31 26
HincII GTy'rAC
Cuts at: 0 273 3300
Size: 273 3027
HindIII A'AGCT_T
Cuts at: 0 1899 3300
Size: 1899 1401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1184 1811 1838 3300
Size: 485 699 627 27 1462
Fragments arranged by size:
1462 699 627 485 27
MscI TGG'CCA
Cuts at: 0 748 3300
Size: 748 2552
MslI CAynn'nnrTG
Cuts at: 0 70 346 1025 2860 3300
Size: 70 276 679 1835 440
Fragments arranged by size:
1835 679 440 276 70
MspA1I CmG'CkG
Cuts at: 0 2468 3300
Size: 2468 832
NaeI GCC'GGC
Cuts at: 0 1702 3300
Size: 1702 1598
NcoI C'CATG_G
Cuts at: 0 1256 3300
Size: 1256 2044
NgoMIV G'CCGG_C
Cuts at: 0 1700 3300
Size: 1700 1600
NspI r_CATG'y
Cuts at: 0 121 351 517 1590 3300
Size: 121 230 166 1073 1710
Fragments arranged by size:
1710 1073 230 166 121
PasI CC'CwG_GG
Cuts at: 0 217 3300
Size: 217 3083
PciI A'CATG_T
Cuts at: 0 347 513 3300
Size: 347 166 2787
Fragments arranged by size:
2787 347 166
PflMI CCAn_nnn'nTGG
Cuts at: 0 287 1262 2448 3300
Size: 287 975 1186 852
Fragments arranged by size:
1186 975 852 287
PfoI T'CCnGG_A
Cuts at: 0 1141 1184 1280 1423 3300
Size: 1141 43 96 143 1877
Fragments arranged by size:
1877 1141 143 96 43
PmlI CAC'GTG
Cuts at: 0 1776 3300
Size: 1776 1524
PpuMI rG'GwC_Cy
Cuts at: 0 1268 1386 1461 3242 3300
Size: 1268 118 75 1781 58
Fragments arranged by size:
1781 1268 118 75 58
PshAI GACnn'nnGTC
Cuts at: 0 1320 3300
Size: 1320 1980
PspOMI G'GGCC_C
Cuts at: 0 226 3300
Size: 226 3074
PstI C_TGCA'G
Cuts at: 0 786 1436 1858 3300
Size: 786 650 422 1442
Fragments arranged by size:
1442 786 650 422
PvuII CAG'CTG
Cuts at: 0 2468 3300
Size: 2468 832
SacI G_AGCT'C
Cuts at: 0 3076 3185 3300
Size: 3076 109 115
Fragments arranged by size:
3076 115 109
SalI G'TCGA_C
Cuts at: 0 271 3300
Size: 271 3029
SanDI GG'GwC_CC
Cuts at: 0 1268 3300
Size: 1268 2032
SapI GCTCTTCn'nnn_
Cuts at: 0 766 897 3300
Size: 766 131 2403
Fragments arranged by size:
2403 766 131
SfcI C'TryA_G
Cuts at: 0 396 435 704 782 1002 1432 1854 2828
Size: 396 39 269 78 220 430 422 974
Cuts at: 2828 3300
Size: 472
Fragments arranged by size:
974 472 430 422 396 269 220 78
39
SmaI CCC'GGG
Cuts at: 0 1340 1507 3300
Size: 1340 167 1793
Fragments arranged by size:
1793 1340 167
SmlI C'TyrA_G
Cuts at: 0 3069 3246 3300
Size: 3069 177 54
Fragments arranged by size:
3069 177 54
SnaBI TAC'GTA
Cuts at: 0 2748 3300
Size: 2748 552
SphI G_CATG'C
Cuts at: 0 121 3300
Size: 121 3179
SspI AAT'ATT
Cuts at: 0 3114 3300
Size: 3114 186
StyI C'CwwG_G
Cuts at: 0 281 625 1241 1256 2560 3300
Size: 281 344 616 15 1304 740
Fragments arranged by size:
1304 740 616 344 281 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 542 605 2378 3300
Size: 542 63 1773 922
Fragments arranged by size:
1773 922 542 63
TatI w'GTAC_w
Cuts at: 0 383 1355 3300
Size: 383 972 1945
Fragments arranged by size:
1945 972 383
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 512 544 3300
Size: 70 32 410 32 2756
Fragments arranged by size:
2756 410 70 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2482 3300
Size: 2482 818
XmaI C'CCGG_G
Cuts at: 0 1338 1505 3300
Size: 1338 167 1795
Fragments arranged by size:
1795 1338 167
XmnI GAAnn'nnTTC
Cuts at: 0 3113 3134 3300
Size: 3113 21 166
Fragments arranged by size:
3113 166 21
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SalI SanDI SapI SfcI SmaI SmlI SnaBI SphI
SspI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AloI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BfrBI BglII BlpI
BmtI BsaI BsaBI BsaHI BsiWI BsrDI BssSI BstEII
BstZ17I ClaI DraI EagI EcoNI EcoRV FalI FseI
FspAI HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SpeI SrfI StuI SwaI
Tth111I XbaI XhoI ZraI