Restriction map for
pF1KA0774
(Linear) (Six-base) MAPSORT of: KIAA0774.seq Check: 2989 from: 1 to: 4137
KIAA0774.orf
With 182 enzymes: SgfI *
March 20, 2014 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 822 1273 2074 3417 4137
Size: 822 451 801 1343 720
Fragments arranged by size:
1343 822 801 720 451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 2116 2139 2390 2456 2728 3052 3106
Size: 256 1860 23 251 66 272 324 54
Cuts at: 3106 3775 4137
Size: 669 362
Fragments arranged by size:
1860 669 362 324 272 256 251 66
54 23
AflII C'TTAA_G
Cuts at: 0 117 2326 4137
Size: 117 2209 1811
Fragments arranged by size:
2209 1811 117
AflIII A'CryG_T
Cuts at: 0 187 822 1775 2047 4137
Size: 187 635 953 272 2090
Fragments arranged by size:
2090 953 635 272 187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2969 4137
Size: 2969 1168
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3256 3288 4137
Size: 3256 32 849
Fragments arranged by size:
3256 849 32
AlwNI CAG_nnn'CTG
Cuts at: 0 874 1992 3327 3399 3645 4137
Size: 874 1118 1335 72 246 492
Fragments arranged by size:
1335 1118 874 492 246 72
ApaI G_GGCC'C
Cuts at: 0 4103 4137
Size: 4103 34
ApaLI G'TGCA_C
Cuts at: 0 1760 2642 4137
Size: 1760 882 1495
Fragments arranged by size:
1760 1495 882
ApoI r'AATT_y
Cuts at: 0 219 259 488 882 1268 2122 3579 4137
Size: 219 40 229 394 386 854 1457 558
Fragments arranged by size:
1457 854 558 394 386 229 219 40
AscI GG'CGCG_CC
Cuts at: 0 3367 4137
Size: 3367 770
AvaI C'yCGr_G
Cuts at: 0 635 2190 4095 4137
Size: 635 1555 1905 42
Fragments arranged by size:
1905 1555 635 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2541 2574 4137
Size: 2541 33 1563
Fragments arranged by size:
2541 1563 33
BamHI G'GATC_C
Cuts at: 0 1068 1817 4137
Size: 1068 749 2320
Fragments arranged by size:
2320 1068 749
BanI G'GyrC_C
Cuts at: 0 1476 4137
Size: 1476 2661
BanII G_rGCy'C
Cuts at: 0 1228 1487 2602 3320 3660 4103 4137
Size: 1228 259 1115 718 340 443 34
Fragments arranged by size:
1228 1115 718 443 340 259 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 1824 2145 2396 3746 3890 4137
Size: 688 1136 321 251 1350 144 247
Fragments arranged by size:
1350 1136 688 321 251 247 144
BbvCI CC'TCA_GC
Cuts at: 0 1596 4137
Size: 1596 2541
BciVI GTATCCnnnnn_n'
Cuts at: 0 2542 2929 4137
Size: 2542 387 1208
Fragments arranged by size:
2542 1208 387
BglI GCCn_nnn'nGGC
Cuts at: 0 2575 3004 4137
Size: 2575 429 1133
Fragments arranged by size:
2575 1133 429
BglII A'GATC_T
Cuts at: 0 3435 4137
Size: 3435 702
BlpI GC'TnA_GC
Cuts at: 0 3008 3400 4137
Size: 3008 392 737
Fragments arranged by size:
3008 737 392
Bme1580I G_kGCm'C
Cuts at: 0 822 1764 2646 3159 4103 4137
Size: 822 942 882 513 944 34
Fragments arranged by size:
944 942 882 822 513 34
BmgBI CAC'GTC
Cuts at: 0 1778 2557 4137
Size: 1778 779 1580
Fragments arranged by size:
1778 1580 779
BmrI ACTGGGnnnn_n'
Cuts at: 0 34 1676 1805 2622 4043 4137
Size: 34 1642 129 817 1421 94
Fragments arranged by size:
1642 1421 817 129 94 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 180 617 3659 4137
Size: 180 437 3042 478
Fragments arranged by size:
3042 478 437 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2586 2604 2747 2757 3283 3304 3349 3724
Size: 2586 18 143 10 526 21 45 375
Cuts at: 3724 3937 4137
Size: 213 200
Fragments arranged by size:
2586 526 375 213 200 143 45 21
18 10
Bpu10I CC'TnA_GC
Cuts at: 0 1481 1596 4137
Size: 1481 115 2541
Fragments arranged by size:
2541 1481 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3699 3832 4137
Size: 3699 133 305
Fragments arranged by size:
3699 305 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 796 2539 4137
Size: 796 1743 1598
Fragments arranged by size:
1743 1598 796
BsaHI Gr'CG_yC
Cuts at: 0 2006 4137
Size: 2006 2131
BsaWI w'CCGG_w
Cuts at: 0 2161 2594 3407 4137
Size: 2161 433 813 730
Fragments arranged by size:
2161 813 730 433
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3256 3286 4072 4102 4137
Size: 3256 30 786 30 35
Fragments arranged by size:
3256 786 35 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 561 1190 1405 1915 2261 2601 3271
Size: 156 405 629 215 510 346 340 670
Cuts at: 3271 3346 3394 3709 4080 4083 4095 4137
Size: 75 48 315 371 3 12 42
Fragments arranged by size:
670 629 510 405 371 346 340 315
215 156 75 48 42 12 3
BseYI C'CCAG_C
Cuts at: 0 2333 3063 3294 4137
Size: 2333 730 231 843
Fragments arranged by size:
2333 843 730 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 858 1301 4137
Size: 858 443 2836
Fragments arranged by size:
2836 858 443
BsiEI CG_ry'CG
Cuts at: 0 2751 4137
Size: 2751 1386
BsiHKAI G_wGCw'C
Cuts at: 0 365 793 848 1764 2602 2646 3320 3386
Size: 365 428 55 916 838 44 674 66
Cuts at: 3386 4137
Size: 751
Fragments arranged by size:
916 838 751 674 428 365 66 55
44
BsmI GAATG_Cn'
Cuts at: 0 141 552 1578 4137
Size: 141 411 1026 2559
Fragments arranged by size:
2559 1026 411 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2563 2613 4137
Size: 2563 50 1524
Fragments arranged by size:
2563 1524 50
Bsp1286I G_dGCh'C
Cuts at: 0 365 793 822 848 1228 1487 1764 2602
Size: 365 428 29 26 380 259 277 838
Cuts at: 2602 2646 3159 3320 3386 3660 4103 4137
Size: 44 513 161 66 274 443 34
Fragments arranged by size:
838 513 443 428 380 365 277 274
259 161 66 44 34 29 26
BspEI T'CCGG_A
Cuts at: 0 2161 2594 4137
Size: 2161 433 1543
Fragments arranged by size:
2161 1543 433
BspHI T'CATG_A
Cuts at: 0 171 3549 4137
Size: 171 3378 588
Fragments arranged by size:
3378 588 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 822 1273 2074 2144 2886 3417 3750 4137
Size: 822 451 801 70 742 531 333 387
Fragments arranged by size:
822 801 742 531 451 387 333 70
BsrBI CCG'CTC
Cuts at: 0 1508 3012 3039 3336 4137
Size: 1508 1504 27 297 801
Fragments arranged by size:
1508 1504 801 297 27
BsrDI GCAATG_nn'
Cuts at: 0 685 2357 3601 4137
Size: 685 1672 1244 536
Fragments arranged by size:
1672 1244 685 536
BsrFI r'CCGG_y
Cuts at: 0 2660 2746 4137
Size: 2660 86 1391
Fragments arranged by size:
2660 1391 86
BssHII G'CGCG_C
Cuts at: 0 3367 4137
Size: 3367 770
BssSI C'ACGA_G
Cuts at: 0 3790 4137
Size: 3790 347
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3021 4137
Size: 3021 1116
BstBI TT'CG_AA
Cuts at: 0 3668 4137
Size: 3668 469
BstEII G'GTnAC_C
Cuts at: 0 1533 2089 3388 4137
Size: 1533 556 1299 749
Fragments arranged by size:
1533 1299 749 556
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1141 3063 4137
Size: 1141 1922 1074
Fragments arranged by size:
1922 1141 1074
BstYI r'GATC_y
Cuts at: 0 960 1068 1817 3435 3857 4137
Size: 960 108 749 1618 422 280
Fragments arranged by size:
1618 960 749 422 280 108
Bsu36I CC'TnA_GG
Cuts at: 0 1236 4137
Size: 1236 2901
BtgI C'CryG_G
Cuts at: 0 3119 4083 4137
Size: 3119 964 54
Fragments arranged by size:
3119 964 54
BtsI GCAGTG_nn'
Cuts at: 0 846 1416 4137
Size: 846 570 2721
Fragments arranged by size:
2721 846 570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1527 1562 4137
Size: 1527 35 2575
Fragments arranged by size:
2575 1527 35
DraI TTT'AAA
Cuts at: 0 2483 4137
Size: 2483 1654
DraIII CAC_nnn'GTG
Cuts at: 0 369 1279 1327 4137
Size: 369 910 48 2810
Fragments arranged by size:
2810 910 369 48
DrdI GACnn_nn'nnGTC
Cuts at: 0 700 3526 4137
Size: 700 2826 611
Fragments arranged by size:
2826 700 611
EaeI y'GGCC_r
Cuts at: 0 1110 1503 2662 2748 3122 4112 4137
Size: 1110 393 1159 86 374 990 25
Fragments arranged by size:
1159 1110 990 393 374 86 25
EagI C'GGCC_G
Cuts at: 0 2748 4137
Size: 2748 1389
EarI CTCTTCn'nnn_
Cuts at: 0 353 1034 1146 1160 1165 2066 2252 3082
Size: 353 681 112 14 5 901 186 830
Cuts at: 3082 3283 3665 3742 3982 4004 4137
Size: 201 382 77 240 22 133
Fragments arranged by size:
901 830 681 382 353 240 201 186
133 112 77 22 14 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 849 2966 3373 4137
Size: 849 2117 407 764
Fragments arranged by size:
2117 849 764 407
EcoICRI GAG'CTC
Cuts at: 0 2600 3318 4137
Size: 2600 718 819
Fragments arranged by size:
2600 819 718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 2116 2139 2390 2456 2586 2604 2728
Size: 256 1860 23 251 66 130 18 124
Cuts at: 2728 2747 2757 3052 3106 3283 3304 3349 3724
Size: 19 10 295 54 177 21 45 375
Cuts at: 3724 3775 3937 4137
Size: 51 162 200
Fragments arranged by size:
1860 375 295 256 251 200 177 162
130 124 66 54 51 45 23 21
19 18 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1971 1996 3376 4137
Size: 1971 25 1380 761
Fragments arranged by size:
1971 1380 761 25
EcoO109I rG'GnC_Cy
Cuts at: 0 1727 1989 2431 4038 4099 4100 4137
Size: 1727 262 442 1607 61 1 37
Fragments arranged by size:
1727 1607 442 262 61 37 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1848 4033 4137
Size: 1848 2185 104
Fragments arranged by size:
2185 1848 104
FseI GG_CCGG'CC
Cuts at: 0 2664 2750 4137
Size: 2664 86 1387
Fragments arranged by size:
2664 1387 86
FspI TGC'GCA
Cuts at: 0 3074 4137
Size: 3074 1063
HaeII r_GCGC'y
Cuts at: 0 2417 2812 4137
Size: 2417 395 1325
Fragments arranged by size:
2417 1325 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 738 750 770 835 867 1033 1065
Size: 718 20 12 20 65 32 166 32
Cuts at: 1065 1711 1743 2379 2411 3531 3563 4137
Size: 646 32 636 32 1120 32 574
Fragments arranged by size:
1120 718 646 636 574 166 65 32
32 32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 26 1182 3415 4137
Size: 26 1156 2233 722
Fragments arranged by size:
2233 1156 722 26
HindIII A'AGCT_T
Cuts at: 0 2489 2920 4137
Size: 2489 431 1217
Fragments arranged by size:
2489 1217 431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 412 1437 2323 3894 3914 4137
Size: 134 278 1025 886 1571 20 223
Fragments arranged by size:
1571 1025 886 278 223 134 20
MscI TGG'CCA
Cuts at: 0 1112 3124 4137
Size: 1112 2012 1013
Fragments arranged by size:
2012 1112 1013
MslI CAynn'nnrTG
Cuts at: 0 1927 4137
Size: 1927 2210
MspA1I CmG'CkG
Cuts at: 0 2833 3018 3063 3345 3396 3702 3930 4085
Size: 2833 185 45 282 51 306 228 155
Cuts at: 4085 4137
Size: 52
Fragments arranged by size:
2833 306 282 228 185 155 52 51
45
NaeI GCC'GGC
Cuts at: 0 2662 2748 4137
Size: 2662 86 1389
Fragments arranged by size:
2662 1389 86
NdeI CA'TA_TG
Cuts at: 0 2034 4137
Size: 2034 2103
NgoMIV G'CCGG_C
Cuts at: 0 2660 2746 4137
Size: 2660 86 1391
Fragments arranged by size:
2660 1391 86
NspI r_CATG'y
Cuts at: 0 191 502 826 1948 2051 4137
Size: 191 311 324 1122 103 2086
Fragments arranged by size:
2086 1122 324 311 191 103
PasI CC'CwG_GG
Cuts at: 0 1186 1992 4137
Size: 1186 806 2145
Fragments arranged by size:
2145 1186 806
PciI A'CATG_T
Cuts at: 0 187 822 2047 4137
Size: 187 635 1225 2090
Fragments arranged by size:
2090 1225 635 187
PflMI CCAn_nnn'nTGG
Cuts at: 0 813 1992 3327 4137
Size: 813 1179 1335 810
Fragments arranged by size:
1335 1179 813 810
PfoI T'CCnGG_A
Cuts at: 0 2544 3320 3953 4137
Size: 2544 776 633 184
Fragments arranged by size:
2544 776 633 184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3256 3288 4137
Size: 3256 32 849
Fragments arranged by size:
3256 849 32
PpuMI rG'GwC_Cy
Cuts at: 0 1727 2431 4038 4137
Size: 1727 704 1607 99
Fragments arranged by size:
1727 1607 704 99
PshAI GACnn'nnGTC
Cuts at: 0 3930 4137
Size: 3930 207
PspOMI G'GGCC_C
Cuts at: 0 4099 4137
Size: 4099 38
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1648 1680 4137
Size: 1648 32 2457
Fragments arranged by size:
2457 1648 32
PstI C_TGCA'G
Cuts at: 0 395 588 833 2141 3344 3377 3509 4137
Size: 395 193 245 1308 1203 33 132 628
Fragments arranged by size:
1308 1203 628 395 245 193 132 33
PvuII CAG'CTG
Cuts at: 0 3018 3063 3345 3396 3702 3930 4137
Size: 3018 45 282 51 306 228 207
Fragments arranged by size:
3018 306 282 228 207 51 45
SacI G_AGCT'C
Cuts at: 0 2602 3320 4137
Size: 2602 718 817
Fragments arranged by size:
2602 817 718
SacII CC_GC'GG
Cuts at: 0 4086 4137
Size: 4086 51
SanDI GG'GwC_CC
Cuts at: 0 1727 4038 4137
Size: 1727 2311 99
Fragments arranged by size:
2311 1727 99
SapI GCTCTTCn'nnn_
Cuts at: 0 353 4004 4137
Size: 353 3651 133
Fragments arranged by size:
3651 353 133
SbfI CC_TGCA'GG
Cuts at: 0 3377 3509 4137
Size: 3377 132 628
Fragments arranged by size:
3377 628 132
SexAI A'CCwGG_T
Cuts at: 0 1323 3611 4137
Size: 1323 2288 526
Fragments arranged by size:
2288 1323 526
SfcI C'TryA_G
Cuts at: 0 391 578 584 829 2137 2457 3340 3373
Size: 391 187 6 245 1308 320 883 33
Cuts at: 3373 3505 4137
Size: 132 632
Fragments arranged by size:
1308 883 632 391 320 245 187 132
33 6
SmaI CCC'GGG
Cuts at: 0 637 2192 4137
Size: 637 1555 1945
Fragments arranged by size:
1945 1555 637
SmlI C'TyrA_G
Cuts at: 0 117 2326 3678 3811 4137
Size: 117 2209 1352 133 326
Fragments arranged by size:
2209 1352 326 133 117
SspI AAT'ATT
Cuts at: 0 1623 4137
Size: 1623 2514
StuI AGG'CCT
Cuts at: 0 3676 4137
Size: 3676 461
StyI C'CwwG_G
Cuts at: 0 506 1055 1140 1376 1733 1844 2146 2738
Size: 506 549 85 236 357 111 302 592
Cuts at: 2738 4137
Size: 1399
Fragments arranged by size:
1399 592 549 506 357 302 236 111
85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1285 1655 1695 2300 2335 2438 2641 2751
Size: 1285 370 40 605 35 103 203 110
Cuts at: 2751 4137
Size: 1386
Fragments arranged by size:
1386 1285 605 370 203 110 103 40
35
TatI w'GTAC_w
Cuts at: 0 825 4137
Size: 825 3312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 734 766 2156 2188 4137
Size: 734 32 1390 32 1949
Fragments arranged by size:
1949 1390 734 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 419 4137
Size: 419 3718
XbaI T'CTAG_A
Cuts at: 0 459 1566 4137
Size: 459 1107 2571
Fragments arranged by size:
2571 1107 459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 513 4137
Size: 513 3624
XmaI C'CCGG_G
Cuts at: 0 635 2190 4137
Size: 635 1555 1947
Fragments arranged by size:
1947 1555 635
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SacII SanDI SapI SbfI SexAI SfcI SmaI SmlI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AseI AsiSI AvrII BbeI BcgI BclI BfrBI BmtI
BsaAI BsaBI BsiWI BsrGI BstZ17I BtgZI ClaI EcoRI
EcoRV FspAI HpaI KasI KpnI MfeI MluI NarI
NcoI NheI NotI NruI NsiI PacI PmeI PmlI
PsiI PspXI PvuI RsrII SalI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI XhoI
XmnI ZraI