Restriction map for
pF1KA0773
(Linear) (Six-base) MAPSORT of: KIAA0773.seq Check: 7287 from: 1 to: 1080
KIAA0773.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AatII G_ACGT'C
Cuts at: 0 1028 1080
Size: 1028 52
AccI GT'mk_AC
Cuts at: 0 997 1080
Size: 997 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 791 1080
Size: 82 709 289
Fragments arranged by size:
709 289 82
AflII C'TTAA_G
Cuts at: 0 211 1080
Size: 211 869
AflIII A'CryG_T
Cuts at: 0 653 1080
Size: 653 427
AhdI GACnn_n'nnGTC
Cuts at: 0 963 1080
Size: 963 117
AlwNI CAG_nnn'CTG
Cuts at: 0 594 1080
Size: 594 486
ApaI G_GGCC'C
Cuts at: 0 246 898 1080
Size: 246 652 182
Fragments arranged by size:
652 246 182
BamHI G'GATC_C
Cuts at: 0 736 1080
Size: 736 344
BanI G'GyrC_C
Cuts at: 0 924 1080
Size: 924 156
BanII G_rGCy'C
Cuts at: 0 246 864 898 1080
Size: 246 618 34 182
Fragments arranged by size:
618 246 182 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 367 506 989 1080
Size: 367 139 483 91
Fragments arranged by size:
483 367 139 91
BbvCI CC'TCA_GC
Cuts at: 0 430 902 1080
Size: 430 472 178
Fragments arranged by size:
472 430 178
BciVI GTATCCnnnnn_n'
Cuts at: 0 402 805 1080
Size: 402 403 275
Fragments arranged by size:
403 402 275
BglI GCCn_nnn'nGGC
Cuts at: 0 727 1080
Size: 727 353
Bme1580I G_kGCm'C
Cuts at: 0 246 898 1080
Size: 246 652 182
Fragments arranged by size:
652 246 182
BmrI ACTGGGnnnn_n'
Cuts at: 0 867 1080
Size: 867 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 982 1080
Size: 844 138 98
Fragments arranged by size:
844 138 98
Bpu10I CC'TnA_GC
Cuts at: 0 430 902 1080
Size: 430 472 178
Fragments arranged by size:
472 430 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1080
Size: 605 475
BsaAI yAC'GTr
Cuts at: 0 654 1080
Size: 654 426
BsaBI GATnn'nnATC
Cuts at: 0 71 1080
Size: 71 1009
BsaHI Gr'CG_yC
Cuts at: 0 467 1025 1080
Size: 467 558 55
Fragments arranged by size:
558 467 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 919 922 946 1072 1080
Size: 919 3 24 126 8
Fragments arranged by size:
919 126 24 8 3
BseYI C'CCAG_C
Cuts at: 0 353 772 840 1080
Size: 353 419 68 240
Fragments arranged by size:
419 353 240 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1063 1080
Size: 88 975 17
Fragments arranged by size:
975 88 17
Bsp1286I G_dGCh'C
Cuts at: 0 246 864 898 1080
Size: 246 618 34 182
Fragments arranged by size:
618 246 182 34
BsrBI CCG'CTC
Cuts at: 0 1003 1080
Size: 1003 77
BsrDI GCAATG_nn'
Cuts at: 0 716 1080
Size: 716 364
BssSI C'ACGA_G
Cuts at: 0 977 1080
Size: 977 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 504 1080
Size: 312 192 576
Fragments arranged by size:
576 312 192
BstYI r'GATC_y
Cuts at: 0 269 736 1080
Size: 269 467 344
Fragments arranged by size:
467 344 269
Bsu36I CC'TnA_GG
Cuts at: 0 929 1035 1080
Size: 929 106 45
Fragments arranged by size:
929 106 45
BtgI C'CryG_G
Cuts at: 0 179 311 518 629 1080
Size: 179 132 207 111 451
Fragments arranged by size:
451 207 179 132 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 449 1080
Size: 449 631
BtsI GCAGTG_nn'
Cuts at: 0 50 100 884 1080
Size: 50 50 784 196
Fragments arranged by size:
784 196 50 50
EaeI y'GGCC_r
Cuts at: 0 124 248 728 1080
Size: 124 124 480 352
Fragments arranged by size:
480 352 124 124
EarI CTCTTCn'nnn_
Cuts at: 0 907 932 1080
Size: 907 25 148
Fragments arranged by size:
907 148 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 791 844 982 1080
Size: 82 709 53 138 98
Fragments arranged by size:
709 138 98 82 53
EcoO109I rG'GnC_Cy
Cuts at: 0 57 242 243 790 894 895 957 1080
Size: 57 185 1 547 104 1 62 123
Fragments arranged by size:
547 185 123 104 62 57 1 1
FspI TGC'GCA
Cuts at: 0 403 1080
Size: 403 677
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 588 620 949 981 1015 1047
Size: 406 32 150 32 329 32 34 32
Cuts at: 1047 1080
Size: 33
Fragments arranged by size:
406 329 150 34 33 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 208 1080
Size: 208 872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1080
Size: 449 631
MscI TGG'CCA
Cuts at: 0 250 1080
Size: 250 830
MslI CAynn'nnrTG
Cuts at: 0 310 389 502 538 1080
Size: 310 79 113 36 542
Fragments arranged by size:
542 310 113 79 36
MspA1I CmG'CkG
Cuts at: 0 370 1080
Size: 370 710
NcoI C'CATG_G
Cuts at: 0 179 311 518 629 1080
Size: 179 132 207 111 451
Fragments arranged by size:
451 207 179 132 111
PasI CC'CwG_GG
Cuts at: 0 660 1080
Size: 660 420
PfoI T'CCnGG_A
Cuts at: 0 16 1080
Size: 16 1064
PpuMI rG'GwC_Cy
Cuts at: 0 790 957 1080
Size: 790 167 123
Fragments arranged by size:
790 167 123
PspOMI G'GGCC_C
Cuts at: 0 242 894 1080
Size: 242 652 186
Fragments arranged by size:
652 242 186
PvuII CAG'CTG
Cuts at: 0 370 1080
Size: 370 710
SfcI C'TryA_G
Cuts at: 0 573 1080
Size: 573 507
SmlI C'TyrA_G
Cuts at: 0 211 620 1080
Size: 211 409 460
Fragments arranged by size:
460 409 211
StyI C'CwwG_G
Cuts at: 0 179 311 518 629 1080
Size: 179 132 207 111 451
Fragments arranged by size:
451 207 179 132 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 565 642 1080
Size: 565 77 438
Fragments arranged by size:
565 438 77
TatI w'GTAC_w
Cuts at: 0 668 1080
Size: 668 412
ZraI GAC'GTC
Cuts at: 0 1026 1080
Size: 1026 54
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AhdI AlwNI ApaI
BamHI BanI BanII BbsI BbvCI BciVI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI
BseYI BsgI Bsp1286I BsrBI BsrDI BssSI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I
FspI Hin4I HindIII MmeI MscI MslI MspA1I NcoI
PasI PfoI PpuMI PspOMI PvuII SfcI SmlI StyI
TaqII TatI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BsaI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI