Restriction map for
pF1KSDA0770
(Linear) (Six-base) MAPSORT of: KIAA0770.seq Check: 680 from: 1 to: 2625
KIAA0770.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1515 2478 2625
Size: 1515 963 147
Fragments arranged by size:
1515 963 147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1696 1709 2625
Size: 1696 13 916
Fragments arranged by size:
1696 916 13
AgeI A'CCGG_T
Cuts at: 0 352 2625
Size: 352 2273
AleI CACnn'nnGTG
Cuts at: 0 1099 2625
Size: 1099 1526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 1088 2625
Size: 823 265 1537
Fragments arranged by size:
1537 823 265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1772 1804 2030 2062 2625
Size: 1772 32 226 32 563
Fragments arranged by size:
1772 563 226 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1593 1602 2625
Size: 1593 9 1023
Fragments arranged by size:
1593 1023 9
ApoI r'AATT_y
Cuts at: 0 424 489 998 1749 2449 2625
Size: 424 65 509 751 700 176
Fragments arranged by size:
751 700 509 424 176 65
AvaI C'yCGr_G
Cuts at: 0 2290 2625
Size: 2290 335
BanII G_rGCy'C
Cuts at: 0 563 590 1508 2046 2625
Size: 563 27 918 538 579
Fragments arranged by size:
918 579 563 538 27
BbvCI CC'TCA_GC
Cuts at: 0 2123 2625
Size: 2123 502
BciVI GTATCCnnnnn_n'
Cuts at: 0 1166 2103 2625
Size: 1166 937 522
Fragments arranged by size:
1166 937 522
BclI T'GATC_A
Cuts at: 0 1269 2625
Size: 1269 1356
BglI GCCn_nnn'nGGC
Cuts at: 0 1393 2625
Size: 1393 1232
BglII A'GATC_T
Cuts at: 0 1692 2625
Size: 1692 933
Bme1580I G_kGCm'C
Cuts at: 0 467 687 1609 2625
Size: 467 220 922 1016
Fragments arranged by size:
1016 922 467 220
BmrI ACTGGGnnnn_n'
Cuts at: 0 213 1900 2625
Size: 213 1687 725
Fragments arranged by size:
1687 725 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1827 2625
Size: 1827 798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 604 907 1432 1937 2254 2272 2625
Size: 322 282 303 525 505 317 18 353
Fragments arranged by size:
525 505 353 322 317 303 282 18
Bpu10I CC'TnA_GC
Cuts at: 0 2123 2625
Size: 2123 502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 2413 2625
Size: 1345 1068 212
Fragments arranged by size:
1345 1068 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 343 2625
Size: 343 2282
BsaAI yAC'GTr
Cuts at: 0 1449 1720 2625
Size: 1449 271 905
Fragments arranged by size:
1449 905 271
BsaBI GATnn'nnATC
Cuts at: 0 2177 2625
Size: 2177 448
BsaWI w'CCGG_w
Cuts at: 0 352 2625
Size: 352 2273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 905 935 2030 2060 2503 2533 2567 2597
Size: 905 30 1095 30 443 30 34 30
Cuts at: 2597 2625
Size: 28
Fragments arranged by size:
1095 905 443 34 30 30 30 30
28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 1450 1816 1948 2140 2524 2625
Size: 373 1077 366 132 192 384 101
Fragments arranged by size:
1077 384 373 366 192 132 101
BseYI C'CCAG_C
Cuts at: 0 1911 1935 2212 2613 2625
Size: 1911 24 277 401 12
Fragments arranged by size:
1911 401 277 24 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1606 2625
Size: 654 952 1019
Fragments arranged by size:
1019 952 654
BsiHKAI G_wGCw'C
Cuts at: 0 563 722 1178 1448 1532 2046 2429 2625
Size: 563 159 456 270 84 514 383 196
Fragments arranged by size:
563 514 456 383 270 196 159 84
BsmI GAATG_Cn'
Cuts at: 0 2216 2625
Size: 2216 409
Bsp1286I G_dGCh'C
Cuts at: 0 467 563 590 687 722 1178 1448 1508
Size: 467 96 27 97 35 456 270 60
Cuts at: 1508 1532 1609 2046 2429 2625
Size: 24 77 437 383 196
Fragments arranged by size:
467 456 437 383 270 196 97 96
77 60 35 27 24
BspHI T'CATG_A
Cuts at: 0 429 2625
Size: 429 2196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1056 1130 1515 1625 2274 2478 2625
Size: 1056 74 385 110 649 204 147
Fragments arranged by size:
1056 649 385 204 147 110 74
BsrBI CCG'CTC
Cuts at: 0 1293 2278 2472 2625
Size: 1293 985 194 153
Fragments arranged by size:
1293 985 194 153
BsrDI GCAATG_nn'
Cuts at: 0 737 1165 2625
Size: 737 428 1460
Fragments arranged by size:
1460 737 428
BsrFI r'CCGG_y
Cuts at: 0 352 1946 1997 2625
Size: 352 1594 51 628
Fragments arranged by size:
1594 628 352 51
BssSI C'ACGA_G
Cuts at: 0 1510 1531 1573 2625
Size: 1510 21 42 1052
Fragments arranged by size:
1510 1052 42 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1593 2222 2625
Size: 1593 629 403
Fragments arranged by size:
1593 629 403
BstXI CCAn_nnnn'nTGG
Cuts at: 0 474 2577 2625
Size: 474 2103 48
Fragments arranged by size:
2103 474 48
BstYI r'GATC_y
Cuts at: 0 203 665 764 1089 1692 2625
Size: 203 462 99 325 603 933
Fragments arranged by size:
933 603 462 325 203 99
Bsu36I CC'TnA_GG
Cuts at: 0 831 2455 2625
Size: 831 1624 170
Fragments arranged by size:
1624 831 170
BtgI C'CryG_G
Cuts at: 0 473 644 713 896 2625
Size: 473 171 69 183 1729
Fragments arranged by size:
1729 473 183 171 69
BtsI GCAGTG_nn'
Cuts at: 0 749 1422 2220 2625
Size: 749 673 798 405
Fragments arranged by size:
798 749 673 405
EaeI y'GGCC_r
Cuts at: 0 710 857 2625
Size: 710 147 1768
Fragments arranged by size:
1768 710 147
EarI CTCTTCn'nnn_
Cuts at: 0 1039 1919 2462 2625
Size: 1039 880 543 163
Fragments arranged by size:
1039 880 543 163
EcoICRI GAG'CTC
Cuts at: 0 561 2044 2625
Size: 561 1483 581
Fragments arranged by size:
1483 581 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 604 907 1432 1696 1709 1937 2254
Size: 322 282 303 525 264 13 228 317
Cuts at: 2254 2272 2625
Size: 18 353
Fragments arranged by size:
525 353 322 317 303 282 264 228
18 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1565 2380 2625
Size: 1565 815 245
Fragments arranged by size:
1565 815 245
EcoO109I rG'GnC_Cy
Cuts at: 0 815 2286 2325 2625
Size: 815 1471 39 300
Fragments arranged by size:
1471 815 300 39
EcoRI G'AATT_C
Cuts at: 0 2449 2625
Size: 2449 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 124 1221 2625
Size: 124 1097 1404
Fragments arranged by size:
1404 1097 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 1797 1829 2567 2599 2625
Size: 1261 32 504 32 738 32 26
Fragments arranged by size:
1261 738 504 32 32 32 26
MfeI C'AATT_G
Cuts at: 0 1243 2625
Size: 1243 1382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 936 2625
Size: 592 344 1689
Fragments arranged by size:
1689 592 344
MscI TGG'CCA
Cuts at: 0 712 859 2625
Size: 712 147 1766
Fragments arranged by size:
1766 712 147
MslI CAynn'nnrTG
Cuts at: 0 678 706 742 1099 1379 2625
Size: 678 28 36 357 280 1246
Fragments arranged by size:
1246 678 357 280 36 28
MspA1I CmG'CkG
Cuts at: 0 582 1915 2617 2625
Size: 582 1333 702 8
Fragments arranged by size:
1333 702 582 8
NaeI GCC'GGC
Cuts at: 0 1999 2625
Size: 1999 626
NcoI C'CATG_G
Cuts at: 0 473 713 896 2625
Size: 473 240 183 1729
Fragments arranged by size:
1729 473 240 183
NgoMIV G'CCGG_C
Cuts at: 0 1997 2625
Size: 1997 628
PflMI CCAn_nnn'nTGG
Cuts at: 0 479 1104 1669 2625
Size: 479 625 565 956
Fragments arranged by size:
956 625 565 479
PfoI T'CCnGG_A
Cuts at: 0 923 1697 2375 2462 2625
Size: 923 774 678 87 163
Fragments arranged by size:
923 774 678 163 87
PmlI CAC'GTG
Cuts at: 0 1449 1720 2625
Size: 1449 271 905
Fragments arranged by size:
1449 905 271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2030 2062 2625
Size: 2030 32 563
Fragments arranged by size:
2030 563 32
PpuMI rG'GwC_Cy
Cuts at: 0 2286 2625
Size: 2286 339
PspXI vC'TCGA_Gb
Cuts at: 0 2290 2625
Size: 2290 335
PstI C_TGCA'G
Cuts at: 0 401 953 1526 1598 1896 2285 2625
Size: 401 552 573 72 298 389 340
Fragments arranged by size:
573 552 401 389 340 298 72
PvuII CAG'CTG
Cuts at: 0 582 1915 2617 2625
Size: 582 1333 702 8
Fragments arranged by size:
1333 702 582 8
SacI G_AGCT'C
Cuts at: 0 563 2046 2625
Size: 563 1483 579
Fragments arranged by size:
1483 579 563
SapI GCTCTTCn'nnn_
Cuts at: 0 2462 2625
Size: 2462 163
SbfI CC_TGCA'GG
Cuts at: 0 1896 2625
Size: 1896 729
ScaI AGT'ACT
Cuts at: 0 2131 2625
Size: 2131 494
SfcI C'TryA_G
Cuts at: 0 117 397 949 1137 1522 1594 1892 2281
Size: 117 280 552 188 385 72 298 389
Cuts at: 2281 2625
Size: 344
Fragments arranged by size:
552 389 385 344 298 280 188 117
72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1393 2625
Size: 1393 1232
SmlI C'TyrA_G
Cuts at: 0 1360 2290 2428 2625
Size: 1360 930 138 197
Fragments arranged by size:
1360 930 197 138
StyI C'CwwG_G
Cuts at: 0 473 713 896 2321 2625
Size: 473 240 183 1425 304
Fragments arranged by size:
1425 473 304 240 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1111 1635 2165 2625
Size: 1111 524 530 460
Fragments arranged by size:
1111 530 524 460
TatI w'GTAC_w
Cuts at: 0 1469 2129 2625
Size: 1469 660 496
Fragments arranged by size:
1469 660 496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2503 2535 2625
Size: 2503 32 90
Fragments arranged by size:
2503 90 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1101 2625
Size: 1101 1524
XhoI C'TCGA_G
Cuts at: 0 2290 2625
Size: 2290 335
XmnI GAAnn'nnTTC
Cuts at: 0 80 2413 2625
Size: 80 2333 212
Fragments arranged by size:
2333 212 80
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI AloI AlwNI ApoI
AvaI BanII BbvCI BciVI BclI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV PflMI PfoI PmlI
PpiI PpuMI PspXI PstI PvuII SacI SapI SbfI
ScaI SfcI SfiI SmlI StyI TaqII TatI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BcgI BfrBI BlpI BmgBI BmtI
BsaHI BsiEI BsiWI BsmBI BspEI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI NarI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI
ZraI