Restriction map for
pF1KA0766
(Linear) (Six-base) MAPSORT of: KIAA0766.seq Check: 6960 from: 1 to: 1821
KIAA0766.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AatII G_ACGT'C
Cuts at: 0 161 1821
Size: 161 1660
AccI GT'mk_AC
Cuts at: 0 35 362 1821
Size: 35 327 1459
Fragments arranged by size:
1459 327 35
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1513 1821
Size: 299 1214 308
Fragments arranged by size:
1214 308 299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1601 1821
Size: 1601 220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1420 1452 1821
Size: 1420 32 369
Fragments arranged by size:
1420 369 32
ApaI G_GGCC'C
Cuts at: 0 111 1821
Size: 111 1710
ApoI r'AATT_y
Cuts at: 0 949 1257 1408 1821
Size: 949 308 151 413
Fragments arranged by size:
949 413 308 151
AvaI C'yCGr_G
Cuts at: 0 58 304 400 1821
Size: 58 246 96 1421
Fragments arranged by size:
1421 246 96 58
BamHI G'GATC_C
Cuts at: 0 458 1821
Size: 458 1363
BanI G'GyrC_C
Cuts at: 0 164 1821
Size: 164 1657
BanII G_rGCy'C
Cuts at: 0 111 311 865 1778 1821
Size: 111 200 554 913 43
Fragments arranged by size:
913 554 200 111 43
BbeI G_GCGC'C
Cuts at: 0 168 1821
Size: 168 1653
BglII A'GATC_T
Cuts at: 0 618 1821
Size: 618 1203
BlpI GC'TnA_GC
Cuts at: 0 179 706 1821
Size: 179 527 1115
Fragments arranged by size:
1115 527 179
Bme1580I G_kGCm'C
Cuts at: 0 111 1821
Size: 111 1710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 308 1821
Size: 308 1513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 1821
Size: 422 1399
Bpu10I CC'TnA_GC
Cuts at: 0 463 1024 1821
Size: 463 561 797
Fragments arranged by size:
797 561 463
BsaBI GATnn'nnATC
Cuts at: 0 881 1376 1733 1821
Size: 881 495 357 88
Fragments arranged by size:
881 495 357 88
BsaHI Gr'CG_yC
Cuts at: 0 10 158 165 1821
Size: 10 148 7 1656
Fragments arranged by size:
1656 148 10 7
BsaWI w'CCGG_w
Cuts at: 0 93 1821
Size: 93 1728
BseYI C'CCAG_C
Cuts at: 0 68 341 1821
Size: 68 273 1480
Fragments arranged by size:
1480 273 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1821
Size: 317 1504
BsiHKAI G_wGCw'C
Cuts at: 0 185 865 1821
Size: 185 680 956
Fragments arranged by size:
956 680 185
BsmBI CGTCTCn'nnnn_
Cuts at: 0 783 1402 1821
Size: 783 619 419
Fragments arranged by size:
783 619 419
Bsp1286I G_dGCh'C
Cuts at: 0 111 185 311 865 1778 1821
Size: 111 74 126 554 913 43
Fragments arranged by size:
913 554 126 111 74 43
BspEI T'CCGG_A
Cuts at: 0 93 1821
Size: 93 1728
BsrBI CCG'CTC
Cuts at: 0 198 1821
Size: 198 1623
BssHII G'CGCG_C
Cuts at: 0 219 1821
Size: 219 1602
BssSI C'ACGA_G
Cuts at: 0 293 1821
Size: 293 1528
BstBI TT'CG_AA
Cuts at: 0 1244 1821
Size: 1244 577
BstYI r'GATC_y
Cuts at: 0 458 618 1821
Size: 458 160 1203
Fragments arranged by size:
1203 458 160
BstZ17I GTA'TAC
Cuts at: 0 363 1821
Size: 363 1458
Bsu36I CC'TnA_GG
Cuts at: 0 1432 1821
Size: 1432 389
BtgI C'CryG_G
Cuts at: 0 260 585 1821
Size: 260 325 1236
Fragments arranged by size:
1236 325 260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 1821
Size: 116 1705
DraI TTT'AAA
Cuts at: 0 516 1043 1482 1821
Size: 516 527 439 339
Fragments arranged by size:
527 516 439 339
EarI CTCTTCn'nnn_
Cuts at: 0 275 1821
Size: 275 1546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 223 1821
Size: 223 1598
EcoICRI GAG'CTC
Cuts at: 0 863 1821
Size: 863 958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 422 1513 1821
Size: 299 123 1091 308
Fragments arranged by size:
1091 308 299 123
EcoO109I rG'GnC_Cy
Cuts at: 0 107 108 595 1428 1821
Size: 107 1 487 833 393
Fragments arranged by size:
833 487 393 107 1
EcoRV GAT'ATC
Cuts at: 0 444 1821
Size: 444 1377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 637 1821
Size: 637 1184
HaeII r_GCGC'y
Cuts at: 0 168 1821
Size: 168 1653
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1380 1412 1821
Size: 1380 32 409
Fragments arranged by size:
1380 409 32
HincII GTy'rAC
Cuts at: 0 36 429 1821
Size: 36 393 1392
Fragments arranged by size:
1392 393 36
KasI G'GCGC_C
Cuts at: 0 164 1821
Size: 164 1657
MfeI C'AATT_G
Cuts at: 0 1017 1821
Size: 1017 804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 705 796 1821
Size: 584 121 91 1025
Fragments arranged by size:
1025 584 121 91
MslI CAynn'nnrTG
Cuts at: 0 376 653 1821
Size: 376 277 1168
Fragments arranged by size:
1168 376 277
MspA1I CmG'CkG
Cuts at: 0 587 1821
Size: 587 1234
NarI GG'CG_CC
Cuts at: 0 165 1821
Size: 165 1656
NspI r_CATG'y
Cuts at: 0 409 1821
Size: 409 1412
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 815 1821
Size: 372 443 1006
Fragments arranged by size:
1006 443 372
PpuMI rG'GwC_Cy
Cuts at: 0 1428 1821
Size: 1428 393
PspOMI G'GGCC_C
Cuts at: 0 107 1821
Size: 107 1714
PstI C_TGCA'G
Cuts at: 0 695 1821
Size: 695 1126
SacI G_AGCT'C
Cuts at: 0 865 1821
Size: 865 956
SacII CC_GC'GG
Cuts at: 0 588 1821
Size: 588 1233
SalI G'TCGA_C
Cuts at: 0 34 1821
Size: 34 1787
SfcI C'TryA_G
Cuts at: 0 691 1821
Size: 691 1130
SfoI GGC'GCC
Cuts at: 0 166 1821
Size: 166 1655
SspI AAT'ATT
Cuts at: 0 1052 1361 1821
Size: 1052 309 460
Fragments arranged by size:
1052 460 309
StuI AGG'CCT
Cuts at: 0 302 1821
Size: 302 1519
StyI C'CwwG_G
Cuts at: 0 533 1821
Size: 533 1288
TatI w'GTAC_w
Cuts at: 0 1237 1821
Size: 1237 584
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 744 776 1821
Size: 744 32 1045
Fragments arranged by size:
1045 744 32
Tth111I GACn'n_nGTC
Cuts at: 0 1007 1821
Size: 1007 814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1767 1821
Size: 1767 54
XmnI GAAnn'nnTTC
Cuts at: 0 620 1169 1821
Size: 620 549 652
Fragments arranged by size:
652 620 549
ZraI GAC'GTC
Cuts at: 0 159 1821
Size: 159 1662
Enzymes that do cut:
AatII AccI AcuI AlfI AloI ApaI ApoI AvaI
BamHI BanI BanII BbeI BglII BlpI Bme1580I BplI
BpmI Bpu10I BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BsrBI BssHII BssSI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI DraI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI
MfeI MmeI MslI MspA1I NarI NspI PflMI PpuMI
PspOMI PstI SacI SacII SalI SfcI SfoI SspI
StuI StyI TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaXI BseRI BsiEI
BsiWI BsmI BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EcoNI EcoRI FseI FspI FspAI HindIII HpaI
KpnI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
TaqII XbaI XhoI XmaI