Restriction map for
pF1KSDA0760
(Linear) (Six-base) MAPSORT of: KIAA0760.seq Check: 2516 from: 1 to: 3672
KIAA0760.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 31 1958 2106 2303 2672 3228 3672
Size: 31 1927 148 197 369 556 444
Fragments arranged by size:
1927 556 444 369 197 148 31
AatII G_ACGT'C
Cuts at: 0 3071 3672
Size: 3071 601
AccI GT'mk_AC
Cuts at: 0 734 833 1885 3672
Size: 734 99 1052 1787
Fragments arranged by size:
1787 1052 734 99
Acc65I G'GTAC_C
Cuts at: 0 2352 3672
Size: 2352 1320
AclI AA'CG_TT
Cuts at: 0 2618 3672
Size: 2618 1054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 618 1126 1792 2172 3672
Size: 478 140 508 666 380 1500
Fragments arranged by size:
1500 666 508 478 380 140
AfeI AGC'GCT
Cuts at: 0 2719 3672
Size: 2719 953
AflII C'TTAA_G
Cuts at: 0 2407 3672
Size: 2407 1265
AflIII A'CryG_T
Cuts at: 0 1144 2459 2696 3672
Size: 1144 1315 237 976
Fragments arranged by size:
1315 1144 976 237
AhdI GACnn_n'nnGTC
Cuts at: 0 48 3672
Size: 48 3624
AleI CACnn'nnGTG
Cuts at: 0 1442 1969 2197 3672
Size: 1442 527 228 1475
Fragments arranged by size:
1475 1442 527 228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 3581 3672
Size: 241 3340 91
Fragments arranged by size:
3340 241 91
AlwNI CAG_nnn'CTG
Cuts at: 0 1860 2955 2997 3054 3672
Size: 1860 1095 42 57 618
Fragments arranged by size:
1860 1095 618 57 42
ApaI G_GGCC'C
Cuts at: 0 81 2683 3278 3672
Size: 81 2602 595 394
Fragments arranged by size:
2602 595 394 81
ApaLI G'TGCA_C
Cuts at: 0 321 489 2029 2119 3571 3672
Size: 321 168 1540 90 1452 101
Fragments arranged by size:
1540 1452 321 168 101 90
AvaI C'yCGr_G
Cuts at: 0 1276 3148 3187 3672
Size: 1276 1872 39 485
Fragments arranged by size:
1872 1276 485 39
BanI G'GyrC_C
Cuts at: 0 2352 2421 2782 2935 3286 3499 3672
Size: 2352 69 361 153 351 213 173
Fragments arranged by size:
2352 361 351 213 173 153 69
BanII G_rGCy'C
Cuts at: 0 81 1633 2471 2683 2924 3155 3278 3672
Size: 81 1552 838 212 241 231 123 394
Fragments arranged by size:
1552 838 394 241 231 212 123 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 720 1114 1649 1995 3224 3522 3672
Size: 466 254 394 535 346 1229 298 150
Fragments arranged by size:
1229 535 466 394 346 298 254 150
BciVI GTATCCnnnnn_n'
Cuts at: 0 1724 3672
Size: 1724 1948
BglI GCCn_nnn'nGGC
Cuts at: 0 2141 3672
Size: 2141 1531
BglII A'GATC_T
Cuts at: 0 1151 3590 3672
Size: 1151 2439 82
Fragments arranged by size:
2439 1151 82
BlpI GC'TnA_GC
Cuts at: 0 2378 2967 3672
Size: 2378 589 705
Fragments arranged by size:
2378 705 589
Bme1580I G_kGCm'C
Cuts at: 0 81 220 325 493 796 805 1813 2033
Size: 81 139 105 168 303 9 1008 220
Cuts at: 2033 2123 2683 2785 3278 3308 3575 3613 3672
Size: 90 560 102 493 30 267 38 59
Fragments arranged by size:
1008 560 493 303 267 220 168 139
105 102 90 81 59 38 30 9
BmgBI CAC'GTC
Cuts at: 0 1883 2124 2454 3672
Size: 1883 241 330 1218
Fragments arranged by size:
1883 1218 330 241
BmrI ACTGGGnnnn_n'
Cuts at: 0 910 1627 1809 2097 3672
Size: 910 717 182 288 1575
Fragments arranged by size:
1575 910 717 288 182
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 831 978 3672
Size: 831 147 2694
Fragments arranged by size:
2694 831 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 126 456 673 852 924 1114 1804
Size: 113 13 330 217 179 72 190 690
Cuts at: 1804 2305 3244 3672
Size: 501 939 428
Fragments arranged by size:
939 690 501 428 330 217 190 179
113 72 13
Bpu10I CC'TnA_GC
Cuts at: 0 806 3672
Size: 806 2866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 430 1720 2908 2938 3013 3672
Size: 288 142 1290 1188 30 75 659
Fragments arranged by size:
1290 1188 659 288 142 75 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 2176 3259 3672
Size: 2176 1083 413
Fragments arranged by size:
2176 1083 413
BsaAI yAC'GTr
Cuts at: 0 663 3672
Size: 663 3009
BsaBI GATnn'nnATC
Cuts at: 0 3370 3672
Size: 3370 302
BsaHI Gr'CG_yC
Cuts at: 0 2974 3068 3672
Size: 2974 94 604
Fragments arranged by size:
2974 604 94
BsaWI w'CCGG_w
Cuts at: 0 1470 3672
Size: 1470 2202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1369 1399 1684 1714 1819 1849 2194 2224
Size: 1369 30 285 30 105 30 345 30
Cuts at: 2224 2458 2488 3672
Size: 234 30 1184
Fragments arranged by size:
1369 1184 345 285 234 105 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 369 504 661 807 2833 2836 3448
Size: 220 149 135 157 146 2026 3 612
Cuts at: 3448 3672
Size: 224
Fragments arranged by size:
2026 612 224 220 157 149 146 135
3
BseYI C'CCAG_C
Cuts at: 0 1130 3672
Size: 1130 2542
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1498 1506 1762 1968 2058 3213 3672
Size: 1192 306 8 256 206 90 1155 459
Fragments arranged by size:
1192 1155 459 306 256 206 90 8
BsiEI CG_ry'CG
Cuts at: 0 366 2330 2557 3162 3672
Size: 366 1964 227 605 510
Fragments arranged by size:
1964 605 510 366 227
BsiHKAI G_wGCw'C
Cuts at: 0 325 493 668 1196 1313 1387 1424 2033
Size: 325 168 175 528 117 74 37 609
Cuts at: 2033 2123 2663 2837 2924 3479 3575 3672
Size: 90 540 174 87 555 96 97
Fragments arranged by size:
609 555 540 528 325 175 174 168
117 97 96 90 87 74 37
BsmI GAATG_Cn'
Cuts at: 0 710 3672
Size: 710 2962
BsmBI CGTCTCn'nnnn_
Cuts at: 0 140 1326 3672
Size: 140 1186 2346
Fragments arranged by size:
2346 1186 140
Bsp1286I G_dGCh'C
Cuts at: 0 81 220 325 493 668 796 805 1196
Size: 81 139 105 168 175 128 9 391
Cuts at: 1196 1313 1387 1424 1633 1813 2033 2123 2471
Size: 117 74 37 209 180 220 90 348
Cuts at: 2471 2663 2683 2785 2837 2924 3155 3278 3308
Size: 192 20 102 52 87 231 123 30
Cuts at: 3308 3479 3575 3613 3672
Size: 171 96 38 59
Fragments arranged by size:
391 348 231 220 209 192 180 175
171 168 139 128 123 117 105 102
96 90 87 81 74 59 52 38
37 30 20 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 31 710 1946 1958 2106 2120 2303 2399
Size: 31 679 1236 12 148 14 183 96
Cuts at: 2399 2672 2864 3228 3672
Size: 273 192 364 444
Fragments arranged by size:
1236 679 444 364 273 192 183 148
96 31 14 12
BsrDI GCAATG_nn'
Cuts at: 0 2058 3672
Size: 2058 1614
BsrFI r'CCGG_y
Cuts at: 0 365 854 2531 3097 3161 3172 3672
Size: 365 489 1677 566 64 11 500
Fragments arranged by size:
1677 566 500 489 365 64 11
BssSI C'ACGA_G
Cuts at: 0 286 409 3412 3672
Size: 286 123 3003 260
Fragments arranged by size:
3003 286 260 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 329 497 716 1271 1382 1727 2081 2400
Size: 329 168 219 555 111 345 354 319
Cuts at: 2400 3335 3672
Size: 935 337
Fragments arranged by size:
935 555 354 345 337 329 319 219
168 111
BstEII G'GTnAC_C
Cuts at: 0 2754 3672
Size: 2754 918
BstYI r'GATC_y
Cuts at: 0 293 449 1097 1151 2927 3365 3590 3672
Size: 293 156 648 54 1776 438 225 82
Fragments arranged by size:
1776 648 438 293 225 156 82 54
Bsu36I CC'TnA_GG
Cuts at: 0 722 2417 3672
Size: 722 1695 1255
Fragments arranged by size:
1695 1255 722
BtgI C'CryG_G
Cuts at: 0 1388 2507 2931 3672
Size: 1388 1119 424 741
Fragments arranged by size:
1388 1119 741 424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3281 3672
Size: 3281 391
BtsI GCAGTG_nn'
Cuts at: 0 402 712 714 745 1260 1326 1437 1449
Size: 402 310 2 31 515 66 111 12
Cuts at: 1449 1458 1980 2215 2835 3201 3672
Size: 9 522 235 620 366 471
Fragments arranged by size:
620 522 515 471 402 366 310 235
111 66 31 12 9 2
DraIII CAC_nnn'GTG
Cuts at: 0 497 1896 1991 2313 2754 3672
Size: 497 1399 95 322 441 918
Fragments arranged by size:
1399 918 497 441 322 95
EaeI y'GGCC_r
Cuts at: 0 572 1085 2150 2342 2363 2554 2988 3449
Size: 572 513 1065 192 21 191 434 461
Cuts at: 3449 3672
Size: 223
Fragments arranged by size:
1065 572 513 461 434 223 192 191
21
EagI C'GGCC_G
Cuts at: 0 2554 3672
Size: 2554 1118
EarI CTCTTCn'nnn_
Cuts at: 0 274 346 3672
Size: 274 72 3326
Fragments arranged by size:
3326 274 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 712 3672
Size: 712 2960
EcoICRI GAG'CTC
Cuts at: 0 2922 3672
Size: 2922 750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 126 456 478 618 673 852 924
Size: 113 13 330 22 140 55 179 72
Cuts at: 924 1114 1126 1792 1804 2172 2305 3244 3672
Size: 190 12 666 12 368 133 939 428
Fragments arranged by size:
939 666 428 368 330 190 179 140
133 113 72 55 22 13 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 1993 3672
Size: 202 1791 1679
Fragments arranged by size:
1791 1679 202
EcoO109I rG'GnC_Cy
Cuts at: 0 2679 2680 3051 3275 3672
Size: 2679 1 371 224 397
Fragments arranged by size:
2679 397 371 224 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 525 621 1089 2187 3642 3672
Size: 525 96 468 1098 1455 30
Fragments arranged by size:
1455 1098 525 468 96 30
FspI TGC'GCA
Cuts at: 0 2860 3672
Size: 2860 812
HaeII r_GCGC'y
Cuts at: 0 929 947 1691 2721 3119 3672
Size: 929 18 744 1030 398 553
Fragments arranged by size:
1030 929 744 553 398 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 34 66 142 174 1819 1851 2557
Size: 27 7 32 76 32 1645 32 706
Cuts at: 2557 2589 3672
Size: 32 1083
Fragments arranged by size:
1645 1083 706 76 32 32 32 32
27 7
HincII GTy'rAC
Cuts at: 0 735 1886 3672
Size: 735 1151 1786
Fragments arranged by size:
1786 1151 735
HindIII A'AGCT_T
Cuts at: 0 3061 3672
Size: 3061 611
KpnI G_GTAC'C
Cuts at: 0 2356 3672
Size: 2356 1316
MluI A'CGCG_T
Cuts at: 0 2459 3672
Size: 2459 1213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1769 2444 3672
Size: 560 1209 675 1228
Fragments arranged by size:
1228 1209 675 560
MscI TGG'CCA
Cuts at: 0 574 2365 2990 3672
Size: 574 1791 625 682
Fragments arranged by size:
1791 682 625 574
MslI CAynn'nnrTG
Cuts at: 0 169 1442 1969 2026 2051 2197 2512 3238
Size: 169 1273 527 57 25 146 315 726
Cuts at: 3238 3324 3672
Size: 86 348
Fragments arranged by size:
1273 726 527 348 315 169 146 86
57 25
MspA1I CmG'CkG
Cuts at: 0 86 684 990 1674 2072 2882 2971 3672
Size: 86 598 306 684 398 810 89 701
Fragments arranged by size:
810 701 684 598 398 306 89 86
NaeI GCC'GGC
Cuts at: 0 3099 3174 3672
Size: 3099 75 498
Fragments arranged by size:
3099 498 75
NcoI C'CATG_G
Cuts at: 0 1388 2507 2931 3672
Size: 1388 1119 424 741
Fragments arranged by size:
1388 1119 741 424
NgoMIV G'CCGG_C
Cuts at: 0 3097 3172 3672
Size: 3097 75 500
Fragments arranged by size:
3097 500 75
NspI r_CATG'y
Cuts at: 0 543 1148 1962 2403 2700 2898 2949 3237
Size: 543 605 814 441 297 198 51 288
Cuts at: 3237 3672
Size: 435
Fragments arranged by size:
814 605 543 441 435 297 288 198
51
PciI A'CATG_T
Cuts at: 0 1144 2696 3672
Size: 1144 1552 976
Fragments arranged by size:
1552 1144 976
PfoI T'CCnGG_A
Cuts at: 0 90 3672
Size: 90 3582
PmlI CAC'GTG
Cuts at: 0 663 3672
Size: 663 3009
PpuMI rG'GwC_Cy
Cuts at: 0 3051 3672
Size: 3051 621
PspOMI G'GGCC_C
Cuts at: 0 77 2679 3274 3672
Size: 77 2602 595 398
Fragments arranged by size:
2602 595 398 77
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1207 1239 3672
Size: 1207 32 2433
Fragments arranged by size:
2433 1207 32
PstI C_TGCA'G
Cuts at: 0 334 533 707 1307 1444 1856 1943 2117
Size: 334 199 174 600 137 412 87 174
Cuts at: 2117 2210 2257 2396 2525 2669 2813 3002 3644
Size: 93 47 139 129 144 144 189 642
Cuts at: 3644 3672
Size: 28
Fragments arranged by size:
642 600 412 334 199 189 174 174
144 144 139 137 129 93 87 47
28
PvuII CAG'CTG
Cuts at: 0 684 3672
Size: 684 2988
SacI G_AGCT'C
Cuts at: 0 2924 3672
Size: 2924 748
SalI G'TCGA_C
Cuts at: 0 733 1884 3672
Size: 733 1151 1788
Fragments arranged by size:
1788 1151 733
SbfI CC_TGCA'GG
Cuts at: 0 1307 2117 2396 3672
Size: 1307 810 279 1276
Fragments arranged by size:
1307 1276 810 279
SexAI A'CCwGG_T
Cuts at: 0 3053 3672
Size: 3053 619
SfcI C'TryA_G
Cuts at: 0 330 529 703 1044 1303 1440 1852 1939
Size: 330 199 174 341 259 137 412 87
Cuts at: 1939 2113 2206 2253 2392 2521 2665 2809 2998
Size: 174 93 47 139 129 144 144 189
Cuts at: 2998 3640 3672
Size: 642 32
Fragments arranged by size:
642 412 341 330 259 199 189 174
174 144 144 139 137 129 93 87
47 32
SmlI C'TyrA_G
Cuts at: 0 267 445 1735 2407 2917 2923 3028 3672
Size: 267 178 1290 672 510 6 105 644
Fragments arranged by size:
1290 672 644 510 267 178 105 6
SphI G_CATG'C
Cuts at: 0 2403 2898 3672
Size: 2403 495 774
Fragments arranged by size:
2403 774 495
StuI AGG'CCT
Cuts at: 0 2263 3672
Size: 2263 1409
StyI C'CwwG_G
Cuts at: 0 1034 1388 1616 2012 2153 2507 2931 3672
Size: 1034 354 228 396 141 354 424 741
Fragments arranged by size:
1034 741 424 396 354 354 228 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 114 177 2344 3121 3304 3649 3672
Size: 114 63 2167 777 183 345 23
Fragments arranged by size:
2167 777 345 183 114 63 23
TatI w'GTAC_w
Cuts at: 0 897 3672
Size: 897 2775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 2194 2226 3672
Size: 691 32 1471 32 1446
Fragments arranged by size:
1471 1446 691 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1881 3672
Size: 1881 1791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 88 119 948 2280 3126 3672
Size: 88 31 829 1332 846 546
Fragments arranged by size:
1332 846 829 546 88 31
XmnI GAAnn'nnTTC
Cuts at: 0 3622 3672
Size: 3622 50
ZraI GAC'GTC
Cuts at: 0 3069 3672
Size: 3069 603
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI
BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KpnI MluI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII
SacI SalI SbfI SexAI SfcI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BmtI BsiWI
BspEI BspHI BsrBI BsrGI BssHII BstBI BstXI BstZ17I
ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspAI
HpaI KasI MfeI NarI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmeI PpiI PshAI PsiI
PspXI PvuI RsrII SacII SanDI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmaI