Restriction map for
pF1KSDA0755
(Linear) (Six-base) MAPSORT of: KIAA0755.seq Check: 2724 from: 1 to: 3096
KIAA0755.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AatII G_ACGT'C
Cuts at: 0 1441 3096
Size: 1441 1655
AccI GT'mk_AC
Cuts at: 0 2772 3096
Size: 2772 324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 1781 2900 3096
Size: 1414 367 1119 196
Fragments arranged by size:
1414 1119 367 196
AflIII A'CryG_T
Cuts at: 0 1106 1634 2678 2777 3096
Size: 1106 528 1044 99 319
Fragments arranged by size:
1106 1044 528 319 99
AhdI GACnn_n'nnGTC
Cuts at: 0 2614 3096
Size: 2614 482
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 3096
Size: 491 32 2573
Fragments arranged by size:
2573 491 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3009 3096
Size: 3009 87
ApoI r'AATT_y
Cuts at: 0 1412 2420 3096
Size: 1412 1008 676
Fragments arranged by size:
1412 1008 676
BamHI G'GATC_C
Cuts at: 0 90 777 3096
Size: 90 687 2319
Fragments arranged by size:
2319 687 90
BanI G'GyrC_C
Cuts at: 0 544 3096
Size: 544 2552
BanII G_rGCy'C
Cuts at: 0 222 587 3096
Size: 222 365 2509
Fragments arranged by size:
2509 365 222
BbsI GAAGACnn'nnnn_
Cuts at: 0 1227 1402 2222 2280 3023 3096
Size: 1227 175 820 58 743 73
Fragments arranged by size:
1227 820 743 175 73 58
BbvCI CC'TCA_GC
Cuts at: 0 44 1952 3096
Size: 44 1908 1144
Fragments arranged by size:
1908 1144 44
BglI GCCn_nnn'nGGC
Cuts at: 0 135 3096
Size: 135 2961
BglII A'GATC_T
Cuts at: 0 2579 3077 3096
Size: 2579 498 19
Fragments arranged by size:
2579 498 19
BlpI GC'TnA_GC
Cuts at: 0 977 3096
Size: 977 2119
Bme1580I G_kGCm'C
Cuts at: 0 83 1114 3096
Size: 83 1031 1982
Fragments arranged by size:
1982 1031 83
BmgBI CAC'GTC
Cuts at: 0 965 1929 3096
Size: 965 964 1167
Fragments arranged by size:
1167 965 964
BmrI ACTGGGnnnn_n'
Cuts at: 0 606 3096
Size: 606 2490
BmtI G_CTAG'C
Cuts at: 0 2320 3096
Size: 2320 776
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1322 3096
Size: 1322 1774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1982 3096
Size: 749 1233 1114
Fragments arranged by size:
1233 1114 749
Bpu10I CC'TnA_GC
Cuts at: 0 44 1952 3096
Size: 44 1908 1144
Fragments arranged by size:
1908 1144 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 3096
Size: 488 2608
BsaAI yAC'GTr
Cuts at: 0 18 3096
Size: 18 3078
BsaHI Gr'CG_yC
Cuts at: 0 1438 3096
Size: 1438 1658
BsaWI w'CCGG_w
Cuts at: 0 2459 3096
Size: 2459 637
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 93 123 827 857 3096
Size: 93 30 704 30 2239
Fragments arranged by size:
2239 704 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 401 452 587 590 848 3096
Size: 401 51 135 3 258 2248
Fragments arranged by size:
2248 401 258 135 51 3
BseYI C'CCAG_C
Cuts at: 0 353 357 1511 1938 3096
Size: 353 4 1154 427 1158
Fragments arranged by size:
1158 1154 427 353 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 722 3096
Size: 293 429 2374
Fragments arranged by size:
2374 429 293
BsiHKAI G_wGCw'C
Cuts at: 0 222 3096
Size: 222 2874
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1429 3096
Size: 1429 1667
Bsp1286I G_dGCh'C
Cuts at: 0 83 222 587 1114 3096
Size: 83 139 365 527 1982
Fragments arranged by size:
1982 527 365 139 83
BspHI T'CATG_A
Cuts at: 0 1331 2618 3096
Size: 1331 1287 478
Fragments arranged by size:
1331 1287 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2715 3096
Size: 2715 381
BsrDI GCAATG_nn'
Cuts at: 0 451 2869 3096
Size: 451 2418 227
Fragments arranged by size:
2418 451 227
BsrGI T'GTAC_A
Cuts at: 0 569 940 2531 3096
Size: 569 371 1591 565
Fragments arranged by size:
1591 569 565 371
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1094 3096
Size: 1094 2002
BstEII G'GTnAC_C
Cuts at: 0 232 3096
Size: 232 2864
BstXI CCAn_nnnn'nTGG
Cuts at: 0 205 2631 3096
Size: 205 2426 465
Fragments arranged by size:
2426 465 205
BstYI r'GATC_y
Cuts at: 0 90 499 777 869 1258 2579 3077 3096
Size: 90 409 278 92 389 1321 498 19
Fragments arranged by size:
1321 498 409 389 278 92 90 19
BtgI C'CryG_G
Cuts at: 0 2183 2624 3096
Size: 2183 441 472
Fragments arranged by size:
2183 472 441
BtsI GCAGTG_nn'
Cuts at: 0 2183 3096
Size: 2183 913
ClaI AT'CG_AT
Cuts at: 0 2162 3096
Size: 2162 934
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1433 1468 3096
Size: 1433 35 1628
Fragments arranged by size:
1628 1433 35
DraI TTT'AAA
Cuts at: 0 2382 3096
Size: 2382 714
EaeI y'GGCC_r
Cuts at: 0 1862 3096
Size: 1862 1234
EarI CTCTTCn'nnn_
Cuts at: 0 635 1418 1427 1492 1906 3045 3096
Size: 635 783 9 65 414 1139 51
Fragments arranged by size:
1139 783 635 414 65 51 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 743 3096
Size: 743 2353
EcoICRI GAG'CTC
Cuts at: 0 220 3096
Size: 220 2876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1414 1781 1982 2900 3096
Size: 749 665 367 201 918 196
Fragments arranged by size:
918 749 665 367 201 196
EcoNI CCTnn'n_nnAGG
Cuts at: 0 53 632 3096
Size: 53 579 2464
Fragments arranged by size:
2464 579 53
EcoO109I rG'GnC_Cy
Cuts at: 0 136 178 256 328 424 733 3096
Size: 136 42 78 72 96 309 2363
Fragments arranged by size:
2363 309 136 96 78 72 42
EcoRV GAT'ATC
Cuts at: 0 658 1147 3096
Size: 658 489 1949
Fragments arranged by size:
1949 658 489
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 735 767 769 801 854 886
Size: 571 32 132 32 2 32 53 32
Cuts at: 886 1918 1950 2216 2248 2274 2306 2334 2366
Size: 1032 32 266 32 26 32 28 32
Cuts at: 2366 2564 2596 2600 2632 3096
Size: 198 32 4 32 464
Fragments arranged by size:
1032 571 464 266 198 132 53 32
32 32 32 32 32 32 32 32
32 28 26 4 2
HincII GTy'rAC
Cuts at: 0 7 270 1581 3096
Size: 7 263 1311 1515
Fragments arranged by size:
1515 1311 263 7
MfeI C'AATT_G
Cuts at: 0 2963 3096
Size: 2963 133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1221 1521 1544 1598 2955 3024 3096
Size: 136 1085 300 23 54 1357 69 72
Fragments arranged by size:
1357 1085 300 136 72 69 54 23
MscI TGG'CCA
Cuts at: 0 1864 3096
Size: 1864 1232
MslI CAynn'nnrTG
Cuts at: 0 2623 2629 2863 3096
Size: 2623 6 234 233
Fragments arranged by size:
2623 234 233 6
MspA1I CmG'CkG
Cuts at: 0 357 2613 3087 3096
Size: 357 2256 474 9
Fragments arranged by size:
2256 474 357 9
NcoI C'CATG_G
Cuts at: 0 2624 3096
Size: 2624 472
NdeI CA'TA_TG
Cuts at: 0 1381 2444 3096
Size: 1381 1063 652
Fragments arranged by size:
1381 1063 652
NheI G'CTAG_C
Cuts at: 0 2316 3096
Size: 2316 780
NspI r_CATG'y
Cuts at: 0 292 1110 1638 2456 2781 3096
Size: 292 818 528 818 325 315
Fragments arranged by size:
818 818 528 325 315 292
PciI A'CATG_T
Cuts at: 0 1106 1634 2777 3096
Size: 1106 528 1143 319
Fragments arranged by size:
1143 1106 528 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 190 645 1203 2448 3096
Size: 190 455 558 1245 648
Fragments arranged by size:
1245 648 558 455 190
PfoI T'CCnGG_A
Cuts at: 0 189 2414 3096
Size: 189 2225 682
Fragments arranged by size:
2225 682 189
PpuMI rG'GwC_Cy
Cuts at: 0 178 328 424 733 3096
Size: 178 150 96 309 2363
Fragments arranged by size:
2363 309 178 150 96
PsiI TTA'TAA
Cuts at: 0 2330 3096
Size: 2330 766
PstI C_TGCA'G
Cuts at: 0 2308 2487 3096
Size: 2308 179 609
Fragments arranged by size:
2308 609 179
PvuII CAG'CTG
Cuts at: 0 357 2613 3087 3096
Size: 357 2256 474 9
Fragments arranged by size:
2256 474 357 9
SacI G_AGCT'C
Cuts at: 0 222 3096
Size: 222 2874
SanDI GG'GwC_CC
Cuts at: 0 178 424 3096
Size: 178 246 2672
Fragments arranged by size:
2672 246 178
SapI GCTCTTCn'nnn_
Cuts at: 0 1418 3045 3096
Size: 1418 1627 51
Fragments arranged by size:
1627 1418 51
SfcI C'TryA_G
Cuts at: 0 887 2304 2483 3096
Size: 887 1417 179 613
Fragments arranged by size:
1417 887 613 179
SmlI C'TyrA_G
Cuts at: 0 503 3096
Size: 503 2593
SspI AAT'ATT
Cuts at: 0 2753 2828 3096
Size: 2753 75 268
Fragments arranged by size:
2753 268 75
StuI AGG'CCT
Cuts at: 0 59 1102 3096
Size: 59 1043 1994
Fragments arranged by size:
1994 1043 59
StyI C'CwwG_G
Cuts at: 0 644 909 1865 2624 2820 3096
Size: 644 265 956 759 196 276
Fragments arranged by size:
956 759 644 276 265 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 367 537 637 2617 3096
Size: 367 170 100 1980 479
Fragments arranged by size:
1980 479 367 170 100
TatI w'GTAC_w
Cuts at: 0 569 940 2531 2559 2695 3096
Size: 569 371 1591 28 136 401
Fragments arranged by size:
1591 569 401 371 136 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 93 125 2454 2486 3096
Size: 93 32 2329 32 610
Fragments arranged by size:
2329 610 93 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 427 1872 3096
Size: 427 1445 1224
Fragments arranged by size:
1445 1224 427
XmnI GAAnn'nnTTC
Cuts at: 0 1412 1729 3096
Size: 1412 317 1367
Fragments arranged by size:
1412 1367 317
ZraI GAC'GTC
Cuts at: 0 1439 3096
Size: 1439 1657
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AloI AlwNI ApoI
BamHI BanI BanII BbsI BbvCI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtsI ClaI CspCI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I
HincII MfeI MmeI MscI MslI MspA1I NcoI NdeI
NheI NspI PciI PflMI PfoI PpuMI PsiI PstI
PvuII SacI SanDI SapI SfcI SmlI SspI StuI
StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BclI BfrBI BsaI BsaBI BsiEI
BsiWI BsmI BspEI BsrBI BsrFI BssHII BssSI BstBI
BstZ17I Bsu36I BtgZI DraIII DrdI EagI EcoRI FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI NaeI NarI NgoMIV NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XhoI XmaI