Restriction map for
pF1KA0750
(Linear) (Six-base) MAPSORT of: KIAA0750.seq Check: 546 from: 1 to: 3372
KIAA0750.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AatII G_ACGT'C
Cuts at: 0 653 3372
Size: 653 2719
Acc65I G'GTAC_C
Cuts at: 0 79 1409 3372
Size: 79 1330 1963
Fragments arranged by size:
1963 1330 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 2783 2909 3372
Size: 590 2193 126 463
Fragments arranged by size:
2193 590 463 126
AflIII A'CryG_T
Cuts at: 0 1388 2999 3001 3021 3372
Size: 1388 1611 2 20 351
Fragments arranged by size:
1611 1388 351 20 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 87 2405 3372
Size: 87 2318 967
Fragments arranged by size:
2318 967 87
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2823 2855 3372
Size: 2823 32 517
Fragments arranged by size:
2823 517 32
AlwNI CAG_nnn'CTG
Cuts at: 0 921 2166 3092 3372
Size: 921 1245 926 280
Fragments arranged by size:
1245 926 921 280
ApaLI G'TGCA_C
Cuts at: 0 249 3372
Size: 249 3123
ApoI r'AATT_y
Cuts at: 0 607 706 817 2463 2584 3145 3372
Size: 607 99 111 1646 121 561 227
Fragments arranged by size:
1646 607 561 227 121 111 99
AvaI C'yCGr_G
Cuts at: 0 1016 1492 2490 3372
Size: 1016 476 998 882
Fragments arranged by size:
1016 998 882 476
BamHI G'GATC_C
Cuts at: 0 1766 3372
Size: 1766 1606
BanI G'GyrC_C
Cuts at: 0 79 661 1291 1409 1574 1867 3310 3372
Size: 79 582 630 118 165 293 1443 62
Fragments arranged by size:
1443 630 582 293 165 118 79 62
BanII G_rGCy'C
Cuts at: 0 1507 1685 1859 2095 2378 2565 3372
Size: 1507 178 174 236 283 187 807
Fragments arranged by size:
1507 807 283 236 187 178 174
BbvCI CC'TCA_GC
Cuts at: 0 1924 2843 3372
Size: 1924 919 529
Fragments arranged by size:
1924 919 529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2145 2179 3372
Size: 2145 34 1193
Fragments arranged by size:
2145 1193 34
BclI T'GATC_A
Cuts at: 0 2916 3372
Size: 2916 456
BglI GCCn_nnn'nGGC
Cuts at: 0 1212 3372
Size: 1212 2160
BglII A'GATC_T
Cuts at: 0 852 1516 3372
Size: 852 664 1856
Fragments arranged by size:
1856 852 664
BlpI GC'TnA_GC
Cuts at: 0 1822 3043 3372
Size: 1822 1221 329
Fragments arranged by size:
1822 1221 329
Bme1580I G_kGCm'C
Cuts at: 0 253 1236 1294 1870 2363 3372
Size: 253 983 58 576 493 1009
Fragments arranged by size:
1009 983 576 493 253 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 1510 3325 3372
Size: 1510 1815 47
Fragments arranged by size:
1815 1510 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3290 3372
Size: 3290 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1324 1498 1515 1757 2536 2930 3372
Size: 70 1254 174 17 242 779 394 442
Fragments arranged by size:
1254 779 442 394 242 174 70 17
Bpu10I CC'TnA_GC
Cuts at: 0 1679 1924 2407 2843 3372
Size: 1679 245 483 436 529
Fragments arranged by size:
1679 529 483 436 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 877 1222 2362 3372
Size: 142 735 345 1140 1010
Fragments arranged by size:
1140 1010 735 345 142
BsaAI yAC'GTr
Cuts at: 0 71 2364 3002 3030 3372
Size: 71 2293 638 28 342
Fragments arranged by size:
2293 638 342 71 28
BsaBI GATnn'nnATC
Cuts at: 0 730 3372
Size: 730 2642
BsaHI Gr'CG_yC
Cuts at: 0 650 3372
Size: 650 2722
BsaWI w'CCGG_w
Cuts at: 0 2008 3372
Size: 2008 1364
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 317 346 347 376 628 658 2611 2641
Size: 317 29 1 29 252 30 1953 30
Cuts at: 2641 2679 2709 3372
Size: 38 30 663
Fragments arranged by size:
1953 663 317 252 38 30 30 30
29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1527 2194 2311 2312 2693 3244 3247 3372
Size: 1527 667 117 1 381 551 3 125
Fragments arranged by size:
1527 667 551 381 125 117 3 1
BseYI C'CCAG_C
Cuts at: 0 516 1555 2308 2448 2818 3372
Size: 516 1039 753 140 370 554
Fragments arranged by size:
1039 753 554 516 370 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 3372
Size: 867 2505
BsiHKAI G_wGCw'C
Cuts at: 0 253 1484 1685 1859 2188 2254 2369 2378
Size: 253 1231 201 174 329 66 115 9
Cuts at: 2378 3372
Size: 994
Fragments arranged by size:
1231 994 329 253 201 174 115 66
9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2028 2726 3195 3372
Size: 2028 698 469 177
Fragments arranged by size:
2028 698 469 177
Bsp1286I G_dGCh'C
Cuts at: 0 253 1236 1294 1484 1507 1685 1859 1870
Size: 253 983 58 190 23 178 174 11
Cuts at: 1870 2095 2188 2254 2363 2369 2378 2565 3372
Size: 225 93 66 109 6 9 187 807
Fragments arranged by size:
983 807 253 225 190 187 178 174
109 93 66 58 23 11 9 6
BspHI T'CATG_A
Cuts at: 0 2204 3372
Size: 2204 1168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 30 1010 1123 3372
Size: 30 980 113 2249
Fragments arranged by size:
2249 980 113 30
BsrBI CCG'CTC
Cuts at: 0 1155 3372
Size: 1155 2217
BsrFI r'CCGG_y
Cuts at: 0 1334 2241 3372
Size: 1334 907 1131
Fragments arranged by size:
1334 1131 907
BsrGI T'GTAC_A
Cuts at: 0 2906 3372
Size: 2906 466
BssSI C'ACGA_G
Cuts at: 0 1174 3372
Size: 1174 2198
BstAPI GCAn_nnn'nTGC
Cuts at: 0 921 1033 1170 2402 3372
Size: 921 112 137 1232 970
Fragments arranged by size:
1232 970 921 137 112
BstEII G'GTnAC_C
Cuts at: 0 168 3372
Size: 168 3204
BstYI r'GATC_y
Cuts at: 0 852 1516 1766 3372
Size: 852 664 250 1606
Fragments arranged by size:
1606 852 664 250
Bsu36I CC'TnA_GG
Cuts at: 0 2927 3372
Size: 2927 445
BtgI C'CryG_G
Cuts at: 0 711 1217 1236 1781 1883 1990 2150 3372
Size: 711 506 19 545 102 107 160 1222
Fragments arranged by size:
1222 711 545 506 160 107 102 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3149 3372
Size: 3149 223
BtsI GCAGTG_nn'
Cuts at: 0 299 1644 2403 3302 3372
Size: 299 1345 759 899 70
Fragments arranged by size:
1345 899 759 299 70
DraIII CAC_nnn'GTG
Cuts at: 0 263 1432 3372
Size: 263 1169 1940
Fragments arranged by size:
1940 1169 263
DrdI GACnn_nn'nnGTC
Cuts at: 0 934 3372
Size: 934 2438
EaeI y'GGCC_r
Cuts at: 0 669 1097 1748 1931 2168 2573 3113 3299
Size: 669 428 651 183 237 405 540 186
Cuts at: 3299 3372
Size: 73
Fragments arranged by size:
669 651 540 428 405 237 186 183
73
EarI CTCTTCn'nnn_
Cuts at: 0 343 776 1270 1864 1975 2259 2551 3193
Size: 343 433 494 594 111 284 292 642
Cuts at: 3193 3372
Size: 179
Fragments arranged by size:
642 594 494 433 343 292 284 179
111
EcoICRI GAG'CTC
Cuts at: 0 1683 1857 2376 3372
Size: 1683 174 519 996
Fragments arranged by size:
1683 996 519 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 590 1324 1498 1515 1757 2536 2783
Size: 70 520 734 174 17 242 779 247
Cuts at: 2783 2909 2930 3372
Size: 126 21 442
Fragments arranged by size:
779 734 520 442 247 242 174 126
70 21 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 3372
Size: 499 2873
EcoO109I rG'GnC_Cy
Cuts at: 0 283 414 828 1439 3372
Size: 283 131 414 611 1933
Fragments arranged by size:
1933 611 414 283 131
EcoRI G'AATT_C
Cuts at: 0 706 3372
Size: 706 2666
EcoRV GAT'ATC
Cuts at: 0 1548 3372
Size: 1548 1824
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3180 3372
Size: 3180 192
FspI TGC'GCA
Cuts at: 0 298 3294 3372
Size: 298 2996 78
Fragments arranged by size:
2996 298 78
HaeII r_GCGC'y
Cuts at: 0 3050 3372
Size: 3050 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 512 544 1783 1815 2941 2973
Size: 346 32 134 32 1239 32 1126 32
Cuts at: 2973 3372
Size: 399
Fragments arranged by size:
1239 1126 399 346 134 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1524 1608 2760 3372
Size: 1524 84 1152 612
Fragments arranged by size:
1524 1152 612 84
KpnI G_GTAC'C
Cuts at: 0 83 1413 3372
Size: 83 1330 1959
Fragments arranged by size:
1959 1330 83
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1373 3372
Size: 614 759 1999
Fragments arranged by size:
1999 759 614
MscI TGG'CCA
Cuts at: 0 1099 1933 2170 2575 3372
Size: 1099 834 237 405 797
Fragments arranged by size:
1099 834 797 405 237
MslI CAynn'nnrTG
Cuts at: 0 69 536 964 1831 3372
Size: 69 467 428 867 1541
Fragments arranged by size:
1541 867 467 428 69
MspA1I CmG'CkG
Cuts at: 0 766 1050 1170 2163 2448 2734 2847 2870
Size: 766 284 120 993 285 286 113 23
Cuts at: 2870 2887 3083 3372
Size: 17 196 289
Fragments arranged by size:
993 766 289 286 285 284 196 120
113 23 17
NcoI C'CATG_G
Cuts at: 0 1217 2150 3372
Size: 1217 933 1222
Fragments arranged by size:
1222 1217 933
NspI r_CATG'y
Cuts at: 0 1122 1606 3372
Size: 1122 484 1766
Fragments arranged by size:
1766 1122 484
PasI CC'CwG_GG
Cuts at: 0 1399 3372
Size: 1399 1973
PflMI CCAn_nnn'nTGG
Cuts at: 0 1631 3367 3372
Size: 1631 1736 5
Fragments arranged by size:
1736 1631 5
PfoI T'CCnGG_A
Cuts at: 0 364 1531 3372
Size: 364 1167 1841
Fragments arranged by size:
1841 1167 364
PmlI CAC'GTG
Cuts at: 0 71 2364 3002 3372
Size: 71 2293 638 370
Fragments arranged by size:
2293 638 370 71
PpuMI rG'GwC_Cy
Cuts at: 0 283 828 3372
Size: 283 545 2544
Fragments arranged by size:
2544 545 283
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 853 885 3372
Size: 853 32 2487
Fragments arranged by size:
2487 853 32
PstI C_TGCA'G
Cuts at: 0 1029 2531 2886 3088 3372
Size: 1029 1502 355 202 284
Fragments arranged by size:
1502 1029 355 284 202
PvuII CAG'CTG
Cuts at: 0 1050 1170 2163 2448 2734 2847 2887 3372
Size: 1050 120 993 285 286 113 40 485
Fragments arranged by size:
1050 993 485 286 285 120 113 40
SacI G_AGCT'C
Cuts at: 0 1685 1859 2378 3372
Size: 1685 174 519 994
Fragments arranged by size:
1685 994 519 174
SapI GCTCTTCn'nnn_
Cuts at: 0 1270 1864 2259 3372
Size: 1270 594 395 1113
Fragments arranged by size:
1270 1113 594 395
SexAI A'CCwGG_T
Cuts at: 0 1570 3372
Size: 1570 1802
SfcI C'TryA_G
Cuts at: 0 484 617 1025 2443 2527 2882 3084 3372
Size: 484 133 408 1418 84 355 202 288
Fragments arranged by size:
1418 484 408 355 288 202 133 84
SmaI CCC'GGG
Cuts at: 0 1018 3372
Size: 1018 2354
SmlI C'TyrA_G
Cuts at: 0 157 856 1201 1492 2377 3372
Size: 157 699 345 291 885 995
Fragments arranged by size:
995 885 699 345 291 157
StuI AGG'CCT
Cuts at: 0 91 2678 3372
Size: 91 2587 694
Fragments arranged by size:
2587 694 91
StyI C'CwwG_G
Cuts at: 0 164 773 1217 1975 2150 2416 3372
Size: 164 609 444 758 175 266 956
Fragments arranged by size:
956 758 609 444 266 175 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 901 1177 2322 2440 3372
Size: 901 276 1145 118 932
Fragments arranged by size:
1145 932 901 276 118
TatI w'GTAC_w
Cuts at: 0 1385 2906 3372
Size: 1385 1521 466
Fragments arranged by size:
1521 1385 466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 432 464 2611 2643 3372
Size: 317 32 83 32 2147 32 729
Fragments arranged by size:
2147 729 317 83 32 32 32
XbaI T'CTAG_A
Cuts at: 0 558 3372
Size: 558 2814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 2085 3108 3312 3372
Size: 336 1749 1023 204 60
Fragments arranged by size:
1749 1023 336 204 60
XhoI C'TCGA_G
Cuts at: 0 1492 3372
Size: 1492 1880
XmaI C'CCGG_G
Cuts at: 0 1016 3372
Size: 1016 2356
XmnI GAAnn'nnTTC
Cuts at: 0 2044 2546 3372
Size: 2044 502 826
Fragments arranged by size:
2044 826 502
ZraI GAC'GTC
Cuts at: 0 651 3372
Size: 651 2721
Enzymes that do cut:
AatII Acc65I AcuI AflIII AlfI AloI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NcoI NspI PasI
PflMI PfoI PmlI PpuMI PsrI PstI PvuII SacI
SapI SexAI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AleI
ApaI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BciVI BfrBI BmgBI BmtI BsaI BsiEI BsiWI BsmI
BspEI BsrDI BssHII BstBI BstXI BstZ17I ClaI CspCI
DraI EagI EciI FseI FspAI HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I