Restriction map for
pF1KSDA0742
(Linear) (Six-base) MAPSORT of: KIAA0742.seq Check: 2824 from: 1 to: 3963
KIAA0742.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1360 3963
Size: 1360 2603
AccI GT'mk_AC
Cuts at: 0 239 3011 3963
Size: 239 2772 952
Fragments arranged by size:
2772 952 239
AclI AA'CG_TT
Cuts at: 0 1826 3963
Size: 1826 2137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1082 1406 1445 1701 2967 3347 3803
Size: 202 880 324 39 256 1266 380 456
Cuts at: 3803 3963
Size: 160
Fragments arranged by size:
1266 880 456 380 324 256 202 160
39
AflII C'TTAA_G
Cuts at: 0 3133 3439 3963
Size: 3133 306 524
Fragments arranged by size:
3133 524 306
AflIII A'CryG_T
Cuts at: 0 748 3963
Size: 748 3215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 849 3545 3963
Size: 849 2696 418
Fragments arranged by size:
2696 849 418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2195 2227 3963
Size: 2195 32 1736
Fragments arranged by size:
2195 1736 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2389 3963
Size: 2389 1574
ApaI G_GGCC'C
Cuts at: 0 3292 3963
Size: 3292 671
ApoI r'AATT_y
Cuts at: 0 196 308 505 587 995 1034 1142 1446
Size: 196 112 197 82 408 39 108 304
Cuts at: 1446 2667 3243 3401 3559 3835 3963
Size: 1221 576 158 158 276 128
Fragments arranged by size:
1221 576 408 304 276 197 196 158
158 128 112 108 82 39
AseI AT'TA_AT
Cuts at: 0 3316 3963
Size: 3316 647
AvaI C'yCGr_G
Cuts at: 0 3214 3963
Size: 3214 749
BamHI G'GATC_C
Cuts at: 0 376 3963
Size: 376 3587
BanII G_rGCy'C
Cuts at: 0 3292 3741 3963
Size: 3292 449 222
Fragments arranged by size:
3292 449 222
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 782 2309 3655 3963
Size: 457 325 1527 1346 308
Fragments arranged by size:
1527 1346 457 325 308
BclI T'GATC_A
Cuts at: 0 1191 3624 3963
Size: 1191 2433 339
Fragments arranged by size:
2433 1191 339
BglII A'GATC_T
Cuts at: 0 3282 3963
Size: 3282 681
Bme1580I G_kGCm'C
Cuts at: 0 3292 3963
Size: 3292 671
BmgBI CAC'GTC
Cuts at: 0 102 3963
Size: 102 3861
BmrI ACTGGGnnnn_n'
Cuts at: 0 845 2028 3963
Size: 845 1183 1935
Fragments arranged by size:
1935 1183 845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 2341 2348 2957 3782 3963
Size: 1151 1190 7 609 825 181
Fragments arranged by size:
1190 1151 825 609 181 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 2683 2765 3963
Size: 986 1697 82 1198
Fragments arranged by size:
1697 1198 986 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 1871 3963
Size: 1871 2092
BsaWI w'CCGG_w
Cuts at: 0 2063 2654 3963
Size: 2063 591 1309
Fragments arranged by size:
2063 1309 591
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2036 2066 2195 2225 3041 3071 3963
Size: 2036 30 129 30 816 30 892
Fragments arranged by size:
2036 892 816 129 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 691 2447 2813 3963
Size: 57 634 1756 366 1150
Fragments arranged by size:
1756 1150 634 366 57
BseYI C'CCAG_C
Cuts at: 0 95 645 1548 2800 3600 3689 3963
Size: 95 550 903 1252 800 89 274
Fragments arranged by size:
1252 903 800 550 274 95 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1999 2369 3963
Size: 886 1113 370 1594
Fragments arranged by size:
1594 1113 886 370
BsiHKAI G_wGCw'C
Cuts at: 0 8 2357 3516 3741 3963
Size: 8 2349 1159 225 222
Fragments arranged by size:
2349 1159 225 222 8
BsmI GAATG_Cn'
Cuts at: 0 1521 2804 3963
Size: 1521 1283 1159
Fragments arranged by size:
1521 1283 1159
Bsp1286I G_dGCh'C
Cuts at: 0 8 2357 3292 3516 3741 3963
Size: 8 2349 935 224 225 222
Fragments arranged by size:
2349 935 225 224 222 8
BspEI T'CCGG_A
Cuts at: 0 2654 3963
Size: 2654 1309
BspHI T'CATG_A
Cuts at: 0 3621 3963
Size: 3621 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1360 2021 3963
Size: 1360 661 1942
Fragments arranged by size:
1942 1360 661
BsrFI r'CCGG_y
Cuts at: 0 27 2419 3963
Size: 27 2392 1544
Fragments arranged by size:
2392 1544 27
BsrGI T'GTAC_A
Cuts at: 0 324 729 3699 3963
Size: 324 405 2970 264
Fragments arranged by size:
2970 405 324 264
BstEII G'GTnAC_C
Cuts at: 0 1808 3963
Size: 1808 2155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2808 3963
Size: 2808 1155
BstYI r'GATC_y
Cuts at: 0 177 376 3282 3963
Size: 177 199 2906 681
Fragments arranged by size:
2906 681 199 177
Bsu36I CC'TnA_GG
Cuts at: 0 1241 3963
Size: 1241 2722
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 92 3963
Size: 92 3871
BtsI GCAGTG_nn'
Cuts at: 0 2357 3208 3813 3914 3963
Size: 2357 851 605 101 49
Fragments arranged by size:
2357 851 605 101 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1208 1243 3963
Size: 1208 35 2720
Fragments arranged by size:
2720 1208 35
DraI TTT'AAA
Cuts at: 0 551 1451 2537 3963
Size: 551 900 1086 1426
Fragments arranged by size:
1426 1086 900 551
DraIII CAC_nnn'GTG
Cuts at: 0 2022 3963
Size: 2022 1941
EaeI y'GGCC_r
Cuts at: 0 25 1865 2471 3142 3197 3277 3963
Size: 25 1840 606 671 55 80 686
Fragments arranged by size:
1840 686 671 606 80 55 25
EarI CTCTTCn'nnn_
Cuts at: 0 665 2065 2125 3575 3963
Size: 665 1400 60 1450 388
Fragments arranged by size:
1450 1400 665 388 60
EcoICRI GAG'CTC
Cuts at: 0 3739 3963
Size: 3739 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1082 1151 1406 1445 1701 2341 2348
Size: 202 880 69 255 39 256 640 7
Cuts at: 2348 2957 2967 3347 3782 3803 3963
Size: 609 10 380 435 21 160
Fragments arranged by size:
880 640 609 435 380 256 255 202
160 69 39 21 10 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1240 3963
Size: 1240 2723
EcoO109I rG'GnC_Cy
Cuts at: 0 936 3289 3963
Size: 936 2353 674
Fragments arranged by size:
2353 936 674
EcoRI G'AATT_C
Cuts at: 0 2667 3401 3835 3963
Size: 2667 734 434 128
Fragments arranged by size:
2667 734 434 128
EcoRV GAT'ATC
Cuts at: 0 3844 3963
Size: 3844 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1266 1878 3454 3963
Size: 1266 612 1576 509
Fragments arranged by size:
1576 1266 612 509
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 2777 2809 3963
Size: 193 32 2552 32 1154
Fragments arranged by size:
2552 1154 193 32 32
HincII GTy'rAC
Cuts at: 0 669 1922 2496 3963
Size: 669 1253 574 1467
Fragments arranged by size:
1467 1253 669 574
HindIII A'AGCT_T
Cuts at: 0 512 3963
Size: 512 3451
MfeI C'AATT_G
Cuts at: 0 1796 3963
Size: 1796 2167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 2181 2357 3963
Size: 338 1843 176 1606
Fragments arranged by size:
1843 1606 338 176
MscI TGG'CCA
Cuts at: 0 1867 3144 3199 3963
Size: 1867 1277 55 764
Fragments arranged by size:
1867 1277 764 55
MslI CAynn'nnrTG
Cuts at: 0 533 3362 3527 3775 3963
Size: 533 2829 165 248 188
Fragments arranged by size:
2829 533 248 188 165
MspA1I CmG'CkG
Cuts at: 0 95 1166 2389 2486 3963
Size: 95 1071 1223 97 1477
Fragments arranged by size:
1477 1223 1071 97 95
NaeI GCC'GGC
Cuts at: 0 2421 3963
Size: 2421 1542
NgoMIV G'CCGG_C
Cuts at: 0 2419 3963
Size: 2419 1544
NspI r_CATG'y
Cuts at: 0 752 1327 3808 3963
Size: 752 575 2481 155
Fragments arranged by size:
2481 752 575 155
PciI A'CATG_T
Cuts at: 0 748 3963
Size: 748 3215
PfoI T'CCnGG_A
Cuts at: 0 133 1525 2178 3963
Size: 133 1392 653 1785
Fragments arranged by size:
1785 1392 653 133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2195 2227 3963
Size: 2195 32 1736
Fragments arranged by size:
2195 1736 32
PpuMI rG'GwC_Cy
Cuts at: 0 936 3963
Size: 936 3027
PspOMI G'GGCC_C
Cuts at: 0 3288 3963
Size: 3288 675
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 308 340 3963
Size: 308 32 3623
Fragments arranged by size:
3623 308 32
PstI C_TGCA'G
Cuts at: 0 918 1310 3963
Size: 918 392 2653
Fragments arranged by size:
2653 918 392
PvuII CAG'CTG
Cuts at: 0 95 1166 2389 3963
Size: 95 1071 1223 1574
Fragments arranged by size:
1574 1223 1071 95
SacI G_AGCT'C
Cuts at: 0 3741 3963
Size: 3741 222
SapI GCTCTTCn'nnn_
Cuts at: 0 3575 3963
Size: 3575 388
ScaI AGT'ACT
Cuts at: 0 954 3963
Size: 954 3009
SfcI C'TryA_G
Cuts at: 0 240 914 1306 2675 3963
Size: 240 674 392 1369 1288
Fragments arranged by size:
1369 1288 674 392 240
SmlI C'TyrA_G
Cuts at: 0 1001 2698 2780 3133 3439 3963
Size: 1001 1697 82 353 306 524
Fragments arranged by size:
1697 1001 524 353 306 82
StuI AGG'CCT
Cuts at: 0 844 1585 2778 3963
Size: 844 741 1193 1185
Fragments arranged by size:
1193 1185 844 741
StyI C'CwwG_G
Cuts at: 0 984 3963
Size: 984 2979
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2010 2383 3659 3963
Size: 2010 373 1276 304
Fragments arranged by size:
2010 1276 373 304
TatI w'GTAC_w
Cuts at: 0 324 729 910 952 1168 1856 3218 3699
Size: 324 405 181 42 216 688 1362 481
Cuts at: 3699 3963
Size: 264
Fragments arranged by size:
1362 688 481 405 324 264 216 181
42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2036 2068 2699 2731 3963
Size: 2036 32 631 32 1232
Fragments arranged by size:
2036 1232 631 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 611 1827 3515 3963
Size: 611 1216 1688 448
Fragments arranged by size:
1688 1216 611 448
XmnI GAAnn'nnTTC
Cuts at: 0 3086 3963
Size: 3086 877
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AlfI AloI
AlwNI ApaI ApoI AseI AvaI BamHI BanII BbsI
BclI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrFI BsrGI BstEII BstXI BstYI
Bsu36I BtgZI BtsI CspCI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PciI PfoI PpiI PpuMI PspOMI PsrI
PstI PvuII SacI SapI ScaI SfcI SmlI StuI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AleI ApaLI AscI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BlpI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstZ17I BtgI ClaI DrdI EagI EciI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PshAI PsiI PspXI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI ZraI