Restriction map for
pF1KSDA0736
(Linear) (Six-base) MAPSORT of: KIAA0736.seq Check: 529 from: 1 to: 2226
KIAA0736.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2205 2226
Size: 2205 21
AccI GT'mk_AC
Cuts at: 0 632 1790 2226
Size: 632 1158 436
Fragments arranged by size:
1158 632 436
Acc65I G'GTAC_C
Cuts at: 0 1247 2226
Size: 1247 979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 2212 2226
Size: 1141 1071 14
Fragments arranged by size:
1141 1071 14
AfeI AGC'GCT
Cuts at: 0 576 1255 2226
Size: 576 679 971
Fragments arranged by size:
971 679 576
AflIII A'CryG_T
Cuts at: 0 587 1628 2009 2226
Size: 587 1041 381 217
Fragments arranged by size:
1041 587 381 217
AleI CACnn'nnGTG
Cuts at: 0 1453 2226
Size: 1453 773
AlwNI CAG_nnn'CTG
Cuts at: 0 316 702 2220 2226
Size: 316 386 1518 6
Fragments arranged by size:
1518 386 316 6
ApoI r'AATT_y
Cuts at: 0 1299 2226
Size: 1299 927
AvaI C'yCGr_G
Cuts at: 0 305 1462 2207 2226
Size: 305 1157 745 19
Fragments arranged by size:
1157 745 305 19
AvrII C'CTAG_G
Cuts at: 0 1278 2226
Size: 1278 948
BanI G'GyrC_C
Cuts at: 0 348 1247 1777 2226
Size: 348 899 530 449
Fragments arranged by size:
899 530 449 348
BanII G_rGCy'C
Cuts at: 0 1050 1072 2226
Size: 1050 22 1154
Fragments arranged by size:
1154 1050 22
BbeI G_GCGC'C
Cuts at: 0 352 2226
Size: 352 1874
BbsI GAAGACnn'nnnn_
Cuts at: 0 723 819 996 2226
Size: 723 96 177 1230
Fragments arranged by size:
1230 723 177 96
BbvCI CC'TCA_GC
Cuts at: 0 2157 2226
Size: 2157 69
BglI GCCn_nnn'nGGC
Cuts at: 0 1045 1973 2226
Size: 1045 928 253
Fragments arranged by size:
1045 928 253
BglII A'GATC_T
Cuts at: 0 275 2226
Size: 275 1951
BmgBI CAC'GTC
Cuts at: 0 1758 2010 2226
Size: 1758 252 216
Fragments arranged by size:
1758 252 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 176 2226
Size: 176 2050
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 676 2226
Size: 676 1550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1017 1408 1875 2226
Size: 380 637 391 467 351
Fragments arranged by size:
637 467 391 380 351
Bpu10I CC'TnA_GC
Cuts at: 0 56 1163 2157 2226
Size: 56 1107 994 69
Fragments arranged by size:
1107 994 69 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 1293 1546 2226
Size: 396 897 253 680
Fragments arranged by size:
897 680 396 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 11 2197 2226
Size: 11 2186 29
Fragments arranged by size:
2186 29 11
BsaBI GATnn'nnATC
Cuts at: 0 1222 2104 2226
Size: 1222 882 122
Fragments arranged by size:
1222 882 122
BsaHI Gr'CG_yC
Cuts at: 0 349 2226
Size: 349 1877
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 684 714 2226
Size: 684 30 1512
Fragments arranged by size:
1512 684 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 166 215 235 2226
Size: 163 3 49 20 1991
Fragments arranged by size:
1991 163 49 20 3
BseYI C'CCAG_C
Cuts at: 0 571 684 1256 2094 2226
Size: 571 113 572 838 132
Fragments arranged by size:
838 572 571 132 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1629 1714 2123 2226
Size: 1629 85 409 103
Fragments arranged by size:
1629 409 103 85
BsiEI CG_ry'CG
Cuts at: 0 486 2226
Size: 486 1740
BsmI GAATG_Cn'
Cuts at: 0 299 1883 2004 2073 2226
Size: 299 1584 121 69 153
Fragments arranged by size:
1584 299 153 121 69
Bsp1286I G_dGCh'C
Cuts at: 0 1050 1072 2226
Size: 1050 22 1154
Fragments arranged by size:
1154 1050 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1116 1278 2205 2226
Size: 1116 162 927 21
Fragments arranged by size:
1116 927 162 21
BsrBI CCG'CTC
Cuts at: 0 333 2226
Size: 333 1893
BsrDI GCAATG_nn'
Cuts at: 0 50 1985 2226
Size: 50 1935 241
Fragments arranged by size:
1935 241 50
BsrFI r'CCGG_y
Cuts at: 0 680 2226
Size: 680 1546
BstAPI GCAn_nnn'nTGC
Cuts at: 0 702 2220 2226
Size: 702 1518 6
Fragments arranged by size:
1518 702 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 2226
Size: 87 2139
BstYI r'GATC_y
Cuts at: 0 275 1223 1511 2226
Size: 275 948 288 715
Fragments arranged by size:
948 715 288 275
BstZ17I GTA'TAC
Cuts at: 0 1791 2226
Size: 1791 435
BtgI C'CryG_G
Cuts at: 0 36 480 2226
Size: 36 444 1746
Fragments arranged by size:
1746 444 36
BtsI GCAGTG_nn'
Cuts at: 0 703 1436 1826 2226
Size: 703 733 390 400
Fragments arranged by size:
733 703 400 390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2037 2072 2226
Size: 2037 35 154
Fragments arranged by size:
2037 154 35
EaeI y'GGCC_r
Cuts at: 0 77 477 483 1946 2226
Size: 77 400 6 1463 280
Fragments arranged by size:
1463 400 280 77 6
EagI C'GGCC_G
Cuts at: 0 483 2226
Size: 483 1743
EarI CTCTTCn'nnn_
Cuts at: 0 781 1589 2226
Size: 781 808 637
Fragments arranged by size:
808 781 637
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1405 2226
Size: 1405 821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1017 1141 1408 1875 2212 2226
Size: 380 637 124 267 467 337 14
Fragments arranged by size:
637 467 380 337 267 124 14
EcoO109I rG'GnC_Cy
Cuts at: 0 96 209 391 1241 2226
Size: 96 113 182 850 985
Fragments arranged by size:
985 850 182 113 96
HaeII r_GCGC'y
Cuts at: 0 352 526 578 1257 2226
Size: 352 174 52 679 969
Fragments arranged by size:
969 679 352 174 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 684 716 800 832 1185 1213 1217 1245
Size: 684 32 84 32 353 28 4 28
Cuts at: 1245 1404 1426 1436 1458 1603 1635 2226
Size: 159 22 10 22 145 32 591
Fragments arranged by size:
684 591 353 159 145 84 32 32
32 28 28 22 22 10 4
HincII GTy'rAC
Cuts at: 0 723 2015 2226
Size: 723 1292 211
Fragments arranged by size:
1292 723 211
KasI G'GCGC_C
Cuts at: 0 348 2226
Size: 348 1878
KpnI G_GTAC'C
Cuts at: 0 1251 2226
Size: 1251 975
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 629 670 1211 1481 2226
Size: 623 6 41 541 270 745
Fragments arranged by size:
745 623 541 270 41 6
MscI TGG'CCA
Cuts at: 0 479 2226
Size: 479 1747
MslI CAynn'nnrTG
Cuts at: 0 646 1342 1453 2226
Size: 646 696 111 773
Fragments arranged by size:
773 696 646 111
MspA1I CmG'CkG
Cuts at: 0 922 995 2089 2161 2226
Size: 922 73 1094 72 65
Fragments arranged by size:
1094 922 73 72 65
NarI GG'CG_CC
Cuts at: 0 349 2226
Size: 349 1877
NspI r_CATG'y
Cuts at: 0 76 591 618 894 1146 1483 2226
Size: 76 515 27 276 252 337 743
Fragments arranged by size:
743 515 337 276 252 76 27
PasI CC'CwG_GG
Cuts at: 0 397 2226
Size: 397 1829
PciI A'CATG_T
Cuts at: 0 587 2226
Size: 587 1639
PflMI CCAn_nnn'nTGG
Cuts at: 0 1431 2226
Size: 1431 795
PfoI T'CCnGG_A
Cuts at: 0 110 1993 2226
Size: 110 1883 233
Fragments arranged by size:
1883 233 110
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 2226
Size: 1156 32 1038
Fragments arranged by size:
1156 1038 32
PpuMI rG'GwC_Cy
Cuts at: 0 209 391 1241 2226
Size: 209 182 850 985
Fragments arranged by size:
985 850 209 182
PstI C_TGCA'G
Cuts at: 0 2225 2226
Size: 2225 1
PvuII CAG'CTG
Cuts at: 0 922 995 2089 2161 2226
Size: 922 73 1094 72 65
Fragments arranged by size:
1094 922 73 72 65
SanDI GG'GwC_CC
Cuts at: 0 209 391 2226
Size: 209 182 1835
Fragments arranged by size:
1835 209 182
ScaI AGT'ACT
Cuts at: 0 1531 2226
Size: 1531 695
SexAI A'CCwGG_T
Cuts at: 0 1243 2226
Size: 1243 983
SfcI C'TryA_G
Cuts at: 0 2221 2226
Size: 2221 5
SfoI GGC'GCC
Cuts at: 0 350 2226
Size: 350 1876
SmaI CCC'GGG
Cuts at: 0 307 1464 2209 2226
Size: 307 1157 745 17
Fragments arranged by size:
1157 745 307 17
SmlI C'TyrA_G
Cuts at: 0 375 1272 1561 2226
Size: 375 897 289 665
Fragments arranged by size:
897 665 375 289
SphI G_CATG'C
Cuts at: 0 76 618 2226
Size: 76 542 1608
Fragments arranged by size:
1608 542 76
SspI AAT'ATT
Cuts at: 0 121 2226
Size: 121 2105
StuI AGG'CCT
Cuts at: 0 623 1108 2226
Size: 623 485 1118
Fragments arranged by size:
1118 623 485
StyI C'CwwG_G
Cuts at: 0 1278 2132 2172 2226
Size: 1278 854 40 54
Fragments arranged by size:
1278 854 54 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 944 1342 2157 2226
Size: 944 398 815 69
Fragments arranged by size:
944 815 398 69
TatI w'GTAC_w
Cuts at: 0 1529 1688 1720 2226
Size: 1529 159 32 506
Fragments arranged by size:
1529 506 159 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 891 923 2226
Size: 891 32 1303
Fragments arranged by size:
1303 891 32
Tth111I GACn'n_nGTC
Cuts at: 0 106 2226
Size: 106 2120
XmaI C'CCGG_G
Cuts at: 0 305 1462 2207 2226
Size: 305 1157 745 19
Fragments arranged by size:
1157 745 305 19
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI
ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI
BglI BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI BstZ17I BtgI BtsI CspCI EaeI EagI EarI
EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NspI PasI PciI
PflMI PfoI PpiI PpuMI PstI PvuII SanDI ScaI
SexAI SfcI SfoI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI BaeI BamHI BcgI BciVI
BclI BfrBI BlpI Bme1580I BmtI BsaAI BsaWI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI
BstEII Bsu36I BtgZI ClaI DraI DraIII DrdI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI
SwaI XbaI XcmI XhoI XmnI ZraI