Restriction map for
pF1KA0727
(Linear) (Six-base) MAPSORT of: KIAA0727.seq Check: 5344 from: 1 to: 3018
KIAA0727.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 1727 3018
Size: 1727 1291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1664 1943 2778 3018
Size: 1267 397 279 835 240
Fragments arranged by size:
1267 835 397 279 240
AflII C'TTAA_G
Cuts at: 0 415 3018
Size: 415 2603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 371 3018
Size: 371 2647
ApaLI G'TGCA_C
Cuts at: 0 2928 3018
Size: 2928 90
ApoI r'AATT_y
Cuts at: 0 22 736 834 841 1484 1511 1707 1930
Size: 22 714 98 7 643 27 196 223
Cuts at: 1930 2033 3018
Size: 103 985
Fragments arranged by size:
985 714 643 223 196 103 98 27
22 7
AvaI C'yCGr_G
Cuts at: 0 67 566 2449 2988 3010 3018
Size: 67 499 1883 539 22 8
Fragments arranged by size:
1883 539 499 67 22 8
BamHI G'GATC_C
Cuts at: 0 1280 2661 3018
Size: 1280 1381 357
Fragments arranged by size:
1381 1280 357
BanI G'GyrC_C
Cuts at: 0 1820 2131 3018
Size: 1820 311 887
Fragments arranged by size:
1820 887 311
BanII G_rGCy'C
Cuts at: 0 705 2456 3018
Size: 705 1751 562
Fragments arranged by size:
1751 705 562
BbsI GAAGACnn'nnnn_
Cuts at: 0 707 2032 2624 2695 3018
Size: 707 1325 592 71 323
Fragments arranged by size:
1325 707 592 323 71
BbvCI CC'TCA_GC
Cuts at: 0 3001 3018
Size: 3001 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1368 1402 3018
Size: 1368 34 1616
Fragments arranged by size:
1616 1368 34
Bme1580I G_kGCm'C
Cuts at: 0 2085 2134 2932 3011 3018
Size: 2085 49 798 79 7
Fragments arranged by size:
2085 798 79 49 7
BmgBI CAC'GTC
Cuts at: 0 2586 3018
Size: 2586 432
BmrI ACTGGGnnnn_n'
Cuts at: 0 368 2893 3018
Size: 368 2525 125
Fragments arranged by size:
2525 368 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 242 3018
Size: 242 2776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1510 2440 3018
Size: 646 864 930 578
Fragments arranged by size:
930 864 646 578
Bpu10I CC'TnA_GC
Cuts at: 0 3001 3018
Size: 3001 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 1612 3018
Size: 459 1153 1406
Fragments arranged by size:
1406 1153 459
BsaI GGTCTCn'nnnn_
Cuts at: 0 213 3018
Size: 213 2805
BsaAI yAC'GTr
Cuts at: 0 2277 3018
Size: 2277 741
BsaHI Gr'CG_yC
Cuts at: 0 1022 2246 3018
Size: 1022 1224 772
Fragments arranged by size:
1224 1022 772
BsaWI w'CCGG_w
Cuts at: 0 950 3018
Size: 950 2068
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 73 119 149 2819 2831 2849 2861
Size: 43 30 46 30 2670 12 18 12
Cuts at: 2861 3018
Size: 157
Fragments arranged by size:
2670 157 46 43 30 30 18 12
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 2332 3018
Size: 799 1533 686
Fragments arranged by size:
1533 799 686
BseYI C'CCAG_C
Cuts at: 0 2357 2963 3018
Size: 2357 606 55
Fragments arranged by size:
2357 606 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 2899 2928 3018
Size: 808 2091 29 90
Fragments arranged by size:
2091 808 90 29
BsiHKAI G_wGCw'C
Cuts at: 0 705 1628 2932 3018
Size: 705 923 1304 86
Fragments arranged by size:
1304 923 705 86
BsmI GAATG_Cn'
Cuts at: 0 2372 3018
Size: 2372 646
BsmBI CGTCTCn'nnnn_
Cuts at: 0 63 1013 2938 2940 3018
Size: 63 950 1925 2 78
Fragments arranged by size:
1925 950 78 63 2
Bsp1286I G_dGCh'C
Cuts at: 0 705 1628 2085 2134 2456 2932 3011 3018
Size: 705 923 457 49 322 476 79 7
Fragments arranged by size:
923 705 476 457 322 79 49 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 2396 3018
Size: 470 1926 622
Fragments arranged by size:
1926 622 470
BsrDI GCAATG_nn'
Cuts at: 0 1215 1355 3018
Size: 1215 140 1663
Fragments arranged by size:
1663 1215 140
BsrFI r'CCGG_y
Cuts at: 0 1817 2192 3018
Size: 1817 375 826
Fragments arranged by size:
1817 826 375
BssSI C'ACGA_G
Cuts at: 0 1330 2221 3018
Size: 1330 891 797
Fragments arranged by size:
1330 891 797
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1876 2410 3018
Size: 1876 534 608
Fragments arranged by size:
1876 608 534
BstBI TT'CG_AA
Cuts at: 0 1509 2043 3018
Size: 1509 534 975
Fragments arranged by size:
1509 975 534
BstEII G'GTnAC_C
Cuts at: 0 520 1668 3018
Size: 520 1148 1350
Fragments arranged by size:
1350 1148 520
BstYI r'GATC_y
Cuts at: 0 788 1280 2661 3018
Size: 788 492 1381 357
Fragments arranged by size:
1381 788 492 357
Bsu36I CC'TnA_GG
Cuts at: 0 85 782 2511 3018
Size: 85 697 1729 507
Fragments arranged by size:
1729 697 507 85
BtgI C'CryG_G
Cuts at: 0 1116 2241 2417 2939 3018
Size: 1116 1125 176 522 79
Fragments arranged by size:
1125 1116 522 176 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1544 3018
Size: 1544 1474
ClaI AT'CG_AT
Cuts at: 0 2601 3018
Size: 2601 417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2104 2139 3018
Size: 2104 35 879
Fragments arranged by size:
2104 879 35
DraI TTT'AAA
Cuts at: 0 839 3018
Size: 839 2179
DraIII CAC_nnn'GTG
Cuts at: 0 2226 3018
Size: 2226 792
DrdI GACnn_nn'nnGTC
Cuts at: 0 2782 3018
Size: 2782 236
EaeI y'GGCC_r
Cuts at: 0 77 359 959 2228 2284 3018
Size: 77 282 600 1269 56 734
Fragments arranged by size:
1269 734 600 282 77 56
EarI CTCTTCn'nnn_
Cuts at: 0 171 262 2797 3018
Size: 171 91 2535 221
Fragments arranged by size:
2535 221 171 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1873 3018
Size: 19 1854 1145
Fragments arranged by size:
1854 1145 19
EcoICRI GAG'CTC
Cuts at: 0 703 3018
Size: 703 2315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1267 1510 1664 1943 2440 2778 3018
Size: 646 621 243 154 279 497 338 240
Fragments arranged by size:
646 621 497 338 279 243 240 154
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2392 3018
Size: 2392 626
EcoO109I rG'GnC_Cy
Cuts at: 0 2322 2462 3018
Size: 2322 140 556
Fragments arranged by size:
2322 556 140
EcoRI G'AATT_C
Cuts at: 0 22 736 1511 1707 1930 3018
Size: 22 714 775 196 223 1088
Fragments arranged by size:
1088 775 714 223 196 22
EcoRV GAT'ATC
Cuts at: 0 299 501 1149 3018
Size: 299 202 648 1869
Fragments arranged by size:
1869 648 299 202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 54 961 3018
Size: 54 907 2057
Fragments arranged by size:
2057 907 54
HaeII r_GCGC'y
Cuts at: 0 1050 1599 2199 2874 3018
Size: 1050 549 600 675 144
Fragments arranged by size:
1050 675 600 549 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 119 151 1053 1085 1771 1803
Size: 43 32 44 32 902 32 686 32
Cuts at: 1803 1848 1880 2682 2714 3018
Size: 45 32 802 32 304
Fragments arranged by size:
902 802 686 304 45 44 43 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1326 3018
Size: 1326 1692
HindIII A'AGCT_T
Cuts at: 0 437 597 3018
Size: 437 160 2421
Fragments arranged by size:
2421 437 160
MfeI C'AATT_G
Cuts at: 0 185 3018
Size: 185 2833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1039 2817 3018
Size: 446 593 1778 201
Fragments arranged by size:
1778 593 446 201
MscI TGG'CCA
Cuts at: 0 79 961 2230 2286 3018
Size: 79 882 1269 56 732
Fragments arranged by size:
1269 882 732 79 56
MspA1I CmG'CkG
Cuts at: 0 1239 1272 3018
Size: 1239 33 1746
Fragments arranged by size:
1746 1239 33
NaeI GCC'GGC
Cuts at: 0 1819 3018
Size: 1819 1199
NcoI C'CATG_G
Cuts at: 0 1116 2241 3018
Size: 1116 1125 777
Fragments arranged by size:
1125 1116 777
NgoMIV G'CCGG_C
Cuts at: 0 1817 3018
Size: 1817 1201
NspI r_CATG'y
Cuts at: 0 2148 3018
Size: 2148 870
PflMI CCAn_nnn'nTGG
Cuts at: 0 2226 3018
Size: 2226 792
PmlI CAC'GTG
Cuts at: 0 2277 3018
Size: 2277 741
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 629 661 2046 2078 3018
Size: 629 32 1385 32 940
Fragments arranged by size:
1385 940 629 32 32
PstI C_TGCA'G
Cuts at: 0 1223 2330 3018
Size: 1223 1107 688
Fragments arranged by size:
1223 1107 688
PvuII CAG'CTG
Cuts at: 0 1239 3018
Size: 1239 1779
SacI G_AGCT'C
Cuts at: 0 705 3018
Size: 705 2313
SfcI C'TryA_G
Cuts at: 0 1219 2326 3018
Size: 1219 1107 692
Fragments arranged by size:
1219 1107 692
SmaI CCC'GGG
Cuts at: 0 3012 3018
Size: 3012 6
SmlI C'TyrA_G
Cuts at: 0 415 474 566 1627 3018
Size: 415 59 92 1061 1391
Fragments arranged by size:
1391 1061 415 92 59
SphI G_CATG'C
Cuts at: 0 2148 3018
Size: 2148 870
StyI C'CwwG_G
Cuts at: 0 624 1031 1116 2066 2162 2241 3018
Size: 624 407 85 950 96 79 777
Fragments arranged by size:
950 777 624 407 96 85 79
TatI w'GTAC_w
Cuts at: 0 338 2675 2901 3018
Size: 338 2337 226 117
Fragments arranged by size:
2337 338 226 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2831 2863 3018
Size: 2831 32 155
Fragments arranged by size:
2831 155 32
Tth111I GACn'n_nGTC
Cuts at: 0 55 3018
Size: 55 2963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 3018
Size: 429 2589
XhoI C'TCGA_G
Cuts at: 0 566 3018
Size: 566 2452
XmaI C'CCGG_G
Cuts at: 0 3010 3018
Size: 3010 8
XmnI GAAnn'nnTTC
Cuts at: 0 1409 2854 3018
Size: 1409 1445 164
Fragments arranged by size:
1445 1409 164
Enzymes that do cut:
AccI AcuI AflII AlfI ApaLI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BcgI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI
MscI MspA1I NaeI NcoI NgoMIV NspI PflMI PmlI
PsrI PstI PvuII SacI SfcI SmaI SmlI SphI
StyI TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BbeI BciVI BclI BfrBI BglI BglII BlpI
BmtI BsaBI BsiEI BsiWI BspEI BspHI BsrBI BsrGI
BssHII BstXI BstZ17I BtsI EagI FseI FspI FspAI
HpaI KasI KpnI MluI MslI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI TaqII XbaI ZraI