Restriction map for
pF1KSDA0719
(Linear) (Six-base) MAPSORT of: KIAA0719.seq Check: 2675 from: 1 to: 1824
KIAA0719.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 317 765 989 1546 1824
Size: 250 67 448 224 557 278
Fragments arranged by size:
557 448 278 250 224 67
AflIII A'CryG_T
Cuts at: 0 1697 1824
Size: 1697 127
AleI CACnn'nnGTG
Cuts at: 0 634 1824
Size: 634 1190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1147 1824
Size: 1147 677
AlwNI CAG_nnn'CTG
Cuts at: 0 43 1824
Size: 43 1781
ApaI G_GGCC'C
Cuts at: 0 91 1824
Size: 91 1733
ApoI r'AATT_y
Cuts at: 0 882 1411 1824
Size: 882 529 413
Fragments arranged by size:
882 529 413
AvaI C'yCGr_G
Cuts at: 0 90 279 1824
Size: 90 189 1545
Fragments arranged by size:
1545 189 90
BanI G'GyrC_C
Cuts at: 0 138 160 191 1824
Size: 138 22 31 1633
Fragments arranged by size:
1633 138 31 22
BanII G_rGCy'C
Cuts at: 0 55 91 342 1824
Size: 55 36 251 1482
Fragments arranged by size:
1482 251 55 36
BbeI G_GCGC'C
Cuts at: 0 195 1824
Size: 195 1629
BbsI GAAGACnn'nnnn_
Cuts at: 0 256 1824
Size: 256 1568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1417 1451 1824
Size: 1417 34 373
Fragments arranged by size:
1417 373 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 883 1824
Size: 883 941
BclI T'GATC_A
Cuts at: 0 1242 1824
Size: 1242 582
BglI GCCn_nnn'nGGC
Cuts at: 0 114 274 1824
Size: 114 160 1550
Fragments arranged by size:
1550 160 114
BglII A'GATC_T
Cuts at: 0 1575 1824
Size: 1575 249
Bme1580I G_kGCm'C
Cuts at: 0 91 97 141 433 1824
Size: 91 6 44 292 1391
Fragments arranged by size:
1391 292 91 44 6
BmgBI CAC'GTC
Cuts at: 0 577 1824
Size: 577 1247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1145 1824
Size: 1145 679
BsaBI GATnn'nnATC
Cuts at: 0 1208 1824
Size: 1208 616
BsaHI Gr'CG_yC
Cuts at: 0 192 218 1824
Size: 192 26 1606
Fragments arranged by size:
1606 192 26
BseYI C'CCAG_C
Cuts at: 0 150 809 1824
Size: 150 659 1015
Fragments arranged by size:
1015 659 150
BsiEI CG_ry'CG
Cuts at: 0 134 1824
Size: 134 1690
BsiHKAI G_wGCw'C
Cuts at: 0 55 312 1824
Size: 55 257 1512
Fragments arranged by size:
1512 257 55
BsmI GAATG_Cn'
Cuts at: 0 952 1203 1824
Size: 952 251 621
Fragments arranged by size:
952 621 251
Bsp1286I G_dGCh'C
Cuts at: 0 55 91 97 141 312 342 433 1824
Size: 55 36 6 44 171 30 91 1391
Fragments arranged by size:
1391 171 91 55 44 36 30 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1025 1824
Size: 1025 799
BsrDI GCAATG_nn'
Cuts at: 0 991 1035 1824
Size: 991 44 789
Fragments arranged by size:
991 789 44
BsrGI T'GTAC_A
Cuts at: 0 526 1127 1824
Size: 526 601 697
Fragments arranged by size:
697 601 526
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1149 1824
Size: 1149 675
BstYI r'GATC_y
Cuts at: 0 1575 1824
Size: 1575 249
BtgI C'CryG_G
Cuts at: 0 1649 1824
Size: 1649 175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 1778 1824
Size: 126 1652 46
Fragments arranged by size:
1652 126 46
BtsI GCAGTG_nn'
Cuts at: 0 629 1265 1824
Size: 629 636 559
Fragments arranged by size:
636 629 559
ClaI AT'CG_AT
Cuts at: 0 1187 1824
Size: 1187 637
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1546 1581 1824
Size: 1546 35 243
Fragments arranged by size:
1546 243 35
DraI TTT'AAA
Cuts at: 0 375 1824
Size: 375 1449
EaeI y'GGCC_r
Cuts at: 0 2 1721 1824
Size: 2 1719 103
Fragments arranged by size:
1719 103 2
EarI CTCTTCn'nnn_
Cuts at: 0 1368 1391 1824
Size: 1368 23 433
Fragments arranged by size:
1368 433 23
EcoICRI GAG'CTC
Cuts at: 0 53 1824
Size: 53 1771
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 317 765 989 1546 1824
Size: 250 67 448 224 557 278
Fragments arranged by size:
557 448 278 250 224 67
EcoO109I rG'GnC_Cy
Cuts at: 0 301 1824
Size: 301 1523
EcoRV GAT'ATC
Cuts at: 0 801 1824
Size: 801 1023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1570 1824
Size: 1570 254
HaeII r_GCGC'y
Cuts at: 0 195 1824
Size: 195 1629
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 741 773 1731 1763 1824
Size: 310 32 399 32 958 32 61
Fragments arranged by size:
958 399 310 61 32 32 32
HincII GTy'rAC
Cuts at: 0 450 1824
Size: 450 1374
HindIII A'AGCT_T
Cuts at: 0 1090 1824
Size: 1090 734
KasI G'GCGC_C
Cuts at: 0 191 1824
Size: 191 1633
MscI TGG'CCA
Cuts at: 0 1723 1824
Size: 1723 101
MslI CAynn'nnrTG
Cuts at: 0 634 1129 1320 1696 1824
Size: 634 495 191 376 128
Fragments arranged by size:
634 495 376 191 128
MspA1I CmG'CkG
Cuts at: 0 28 43 120 224 287 1227 1824
Size: 28 15 77 104 63 940 597
Fragments arranged by size:
940 597 104 77 63 28 15
NarI GG'CG_CC
Cuts at: 0 192 1824
Size: 192 1632
NcoI C'CATG_G
Cuts at: 0 1649 1824
Size: 1649 175
NdeI CA'TA_TG
Cuts at: 0 1536 1824
Size: 1536 288
NspI r_CATG'y
Cuts at: 0 675 1128 1134 1701 1824
Size: 675 453 6 567 123
Fragments arranged by size:
675 567 453 123 6
PciI A'CATG_T
Cuts at: 0 1697 1824
Size: 1697 127
PfoI T'CCnGG_A
Cuts at: 0 55 1824
Size: 55 1769
PpuMI rG'GwC_Cy
Cuts at: 0 301 1824
Size: 301 1523
PshAI GACnn'nnGTC
Cuts at: 0 1296 1824
Size: 1296 528
PspOMI G'GGCC_C
Cuts at: 0 87 1824
Size: 87 1737
PvuII CAG'CTG
Cuts at: 0 120 1227 1824
Size: 120 1107 597
Fragments arranged by size:
1107 597 120
SacI G_AGCT'C
Cuts at: 0 55 1824
Size: 55 1769
SfcI C'TryA_G
Cuts at: 0 434 1824
Size: 434 1390
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 274 1824
Size: 274 1550
SfoI GGC'GCC
Cuts at: 0 193 1824
Size: 193 1631
SmaI CCC'GGG
Cuts at: 0 92 281 1824
Size: 92 189 1543
Fragments arranged by size:
1543 189 92
SmlI C'TyrA_G
Cuts at: 0 1160 1824
Size: 1160 664
SphI G_CATG'C
Cuts at: 0 675 1824
Size: 675 1149
SrfI GCCC'GGGC
Cuts at: 0 92 1824
Size: 92 1732
SspI AAT'ATT
Cuts at: 0 370 1824
Size: 370 1454
StyI C'CwwG_G
Cuts at: 0 702 1417 1649 1824
Size: 702 715 232 175
Fragments arranged by size:
715 702 232 175
TatI w'GTAC_w
Cuts at: 0 526 1127 1665 1824
Size: 526 601 538 159
Fragments arranged by size:
601 538 526 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 909 1731 1824
Size: 909 822 93
Fragments arranged by size:
909 822 93
XmaI C'CCGG_G
Cuts at: 0 90 279 1824
Size: 90 189 1545
Fragments arranged by size:
1545 189 90
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII Bme1580I BmgBI BpuEI BsaBI BsaHI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstAPI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII KasI MscI MslI MspA1I NarI NcoI NdeI
NspI PciI PfoI PpuMI PshAI PspOMI PvuII SacI
SfcI SfiI SfoI SmaI SmlI SphI SrfI SspI
StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BfrBI BlpI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BsgI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I DraIII DrdI EagI EciI
EcoNI EcoRI FseI FspI FspAI HpaI KpnI MfeI
MluI MmeI NaeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI