Restriction map for
pF1KSDA0717
(Linear) (Six-base) MAPSORT of: KIAA0717.seq Check: 3127 from: 1 to: 2181
KIAA0717.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 647 2181
Size: 647 1534
AatII G_ACGT'C
Cuts at: 0 596 803 1835 2181
Size: 596 207 1032 346
Fragments arranged by size:
1032 596 346 207
Acc65I G'GTAC_C
Cuts at: 0 365 1130 2009 2181
Size: 365 765 879 172
Fragments arranged by size:
879 765 365 172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2035 2181
Size: 2035 146
AflIII A'CryG_T
Cuts at: 0 1037 1922 2181
Size: 1037 885 259
Fragments arranged by size:
1037 885 259
AleI CACnn'nnGTG
Cuts at: 0 145 2004 2181
Size: 145 1859 177
Fragments arranged by size:
1859 177 145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2104 2136 2181
Size: 2104 32 45
Fragments arranged by size:
2104 45 32
AlwNI CAG_nnn'CTG
Cuts at: 0 135 2181
Size: 135 2046
ApaI G_GGCC'C
Cuts at: 0 922 1274 2181
Size: 922 352 907
Fragments arranged by size:
922 907 352
AvaI C'yCGr_G
Cuts at: 0 206 392 1363 1613 1940 2181
Size: 206 186 971 250 327 241
Fragments arranged by size:
971 327 250 241 206 186
BanI G'GyrC_C
Cuts at: 0 365 690 732 937 1130 1489 1525 2009
Size: 365 325 42 205 193 359 36 484
Cuts at: 2009 2181
Size: 172
Fragments arranged by size:
484 365 359 325 205 193 172 42
36
BanII G_rGCy'C
Cuts at: 0 922 1069 1274 1609 2181
Size: 922 147 205 335 572
Fragments arranged by size:
922 572 335 205 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 1151 2123 2181
Size: 1151 972 58
Fragments arranged by size:
1151 972 58
BclI T'GATC_A
Cuts at: 0 969 2181
Size: 969 1212
BglI GCCn_nnn'nGGC
Cuts at: 0 2094 2181
Size: 2094 87
BglII A'GATC_T
Cuts at: 0 295 845 2181
Size: 295 550 1336
Fragments arranged by size:
1336 550 295
BlpI GC'TnA_GC
Cuts at: 0 1355 2181
Size: 1355 826
Bme1580I G_kGCm'C
Cuts at: 0 99 152 737 766 922 940 1274 2043
Size: 99 53 585 29 156 18 334 769
Cuts at: 2043 2181
Size: 138
Fragments arranged by size:
769 585 334 156 138 99 53 29
18
BmgBI CAC'GTC
Cuts at: 0 213 1038 1449 2181
Size: 213 825 411 732
Fragments arranged by size:
825 732 411 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 570 2181
Size: 117 453 1611
Fragments arranged by size:
1611 453 117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 637 2181
Size: 637 1544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 732 799 1185 1677 2181
Size: 466 266 67 386 492 504
Fragments arranged by size:
504 492 466 386 266 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 2062 2181
Size: 1273 789 119
Fragments arranged by size:
1273 789 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 254 553 2181
Size: 31 223 299 1628
Fragments arranged by size:
1628 299 223 31
BsaHI Gr'CG_yC
Cuts at: 0 593 800 1832 2181
Size: 593 207 1032 349
Fragments arranged by size:
1032 593 349 207
BsaWI w'CCGG_w
Cuts at: 0 1069 2181
Size: 1069 1112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 1609 1639 2181
Size: 730 30 849 30 542
Fragments arranged by size:
849 730 542 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 852 855 2136 2139 2157 2181
Size: 772 80 3 1281 3 18 24
Fragments arranged by size:
1281 772 80 24 18 3 3
BseYI C'CCAG_C
Cuts at: 0 540 912 1060 2181
Size: 540 372 148 1121
Fragments arranged by size:
1121 540 372 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 691 1890 2181
Size: 602 89 1199 291
Fragments arranged by size:
1199 602 291 89
BsiHKAI G_wGCw'C
Cuts at: 0 399 1609 2181
Size: 399 1210 572
Fragments arranged by size:
1210 572 399
BsmBI CGTCTCn'nnnn_
Cuts at: 0 203 237 2181
Size: 203 34 1944
Fragments arranged by size:
1944 203 34
Bsp1286I G_dGCh'C
Cuts at: 0 99 152 399 737 766 922 940 1069
Size: 99 53 247 338 29 156 18 129
Cuts at: 1069 1274 1609 2043 2181
Size: 205 335 434 138
Fragments arranged by size:
434 338 335 247 205 156 138 129
99 53 29 18
BspHI T'CATG_A
Cuts at: 0 1418 2181
Size: 1418 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 647 2181
Size: 437 210 1534
Fragments arranged by size:
1534 437 210
BsrBI CCG'CTC
Cuts at: 0 789 822 2181
Size: 789 33 1359
Fragments arranged by size:
1359 789 33
BsrDI GCAATG_nn'
Cuts at: 0 114 323 672 1340 1349 2181
Size: 114 209 349 668 9 832
Fragments arranged by size:
832 668 349 209 114 9
BsrFI r'CCGG_y
Cuts at: 0 1678 2181
Size: 1678 503
BsrGI T'GTAC_A
Cuts at: 0 1298 2181
Size: 1298 883
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1646 2181
Size: 1646 535
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 570 1000 2006 2181
Size: 540 30 430 1006 175
Fragments arranged by size:
1006 540 430 175 30
BstYI r'GATC_y
Cuts at: 0 295 845 1106 1206 2181
Size: 295 550 261 100 975
Fragments arranged by size:
975 550 295 261 100
BtgI C'CryG_G
Cuts at: 0 71 999 2181
Size: 71 928 1182
Fragments arranged by size:
1182 928 71
ClaI AT'CG_AT
Cuts at: 0 1823 2181
Size: 1823 358
EaeI y'GGCC_r
Cuts at: 0 137 530 1391 1880 1996 2181
Size: 137 393 861 489 116 185
Fragments arranged by size:
861 489 393 185 137 116
EarI CTCTTCn'nnn_
Cuts at: 0 1187 2110 2181
Size: 1187 923 71
Fragments arranged by size:
1187 923 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1441 2181
Size: 1441 740
EcoICRI GAG'CTC
Cuts at: 0 1607 2181
Size: 1607 574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 732 799 1185 1677 2035 2181
Size: 466 266 67 386 492 358 146
Fragments arranged by size:
492 466 386 358 266 146 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 477 778 901 2017 2181
Size: 477 301 123 1116 164
Fragments arranged by size:
1116 477 301 164 123
EcoO109I rG'GnC_Cy
Cuts at: 0 783 918 919 1270 1271 2181
Size: 783 135 1 351 1 910
Fragments arranged by size:
910 783 351 135 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 612 1494 2181
Size: 612 882 687
Fragments arranged by size:
882 687 612
FspI TGC'GCA
Cuts at: 0 829 1381 2181
Size: 829 552 800
Fragments arranged by size:
829 800 552
FspAI rTGC'GCAy
Cuts at: 0 829 2181
Size: 829 1352
HaeII r_GCGC'y
Cuts at: 0 1538 2181
Size: 1538 643
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 386 418 730 762 2181
Size: 386 32 312 32 1419
Fragments arranged by size:
1419 386 312 32 32
HincII GTy'rAC
Cuts at: 0 456 2181
Size: 456 1725
KpnI G_GTAC'C
Cuts at: 0 369 1134 2013 2181
Size: 369 765 879 168
Fragments arranged by size:
879 765 369 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 2181
Size: 404 1777
MscI TGG'CCA
Cuts at: 0 139 532 1393 1998 2181
Size: 139 393 861 605 183
Fragments arranged by size:
861 605 393 183 139
MslI CAynn'nnrTG
Cuts at: 0 145 409 730 808 998 1387 1681 2004
Size: 145 264 321 78 190 389 294 323
Cuts at: 2004 2181
Size: 177
Fragments arranged by size:
389 323 321 294 264 190 177 145
78
MspA1I CmG'CkG
Cuts at: 0 132 675 1562 1878 2037 2181
Size: 132 543 887 316 159 144
Fragments arranged by size:
887 543 316 159 144 132
NcoI C'CATG_G
Cuts at: 0 999 2181
Size: 999 1182
NspI r_CATG'y
Cuts at: 0 148 1653 1686 2181
Size: 148 1505 33 495
Fragments arranged by size:
1505 495 148 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 135 158 777 1812 2181
Size: 135 23 619 1035 369
Fragments arranged by size:
1035 619 369 135 23
PfoI T'CCnGG_A
Cuts at: 0 776 1162 1187 1514 1844 2181
Size: 776 386 25 327 330 337
Fragments arranged by size:
776 386 337 330 327 25
PpuMI rG'GwC_Cy
Cuts at: 0 783 2181
Size: 783 1398
PshAI GACnn'nnGTC
Cuts at: 0 1889 2181
Size: 1889 292
PspOMI G'GGCC_C
Cuts at: 0 918 1270 2181
Size: 918 352 911
Fragments arranged by size:
918 911 352
PspXI vC'TCGA_Gb
Cuts at: 0 1363 2181
Size: 1363 818
PstI C_TGCA'G
Cuts at: 0 644 689 818 2181
Size: 644 45 129 1363
Fragments arranged by size:
1363 644 129 45
PvuII CAG'CTG
Cuts at: 0 132 1562 1878 2181
Size: 132 1430 316 303
Fragments arranged by size:
1430 316 303 132
SacI G_AGCT'C
Cuts at: 0 1609 2181
Size: 1609 572
SapI GCTCTTCn'nnn_
Cuts at: 0 2110 2181
Size: 2110 71
SexAI A'CCwGG_T
Cuts at: 0 1754 2181
Size: 1754 427
SfcI C'TryA_G
Cuts at: 0 640 685 814 2181
Size: 640 45 129 1367
Fragments arranged by size:
1367 640 129 45
SmaI CCC'GGG
Cuts at: 0 1615 2181
Size: 1615 566
SmlI C'TyrA_G
Cuts at: 0 1288 1363 2077 2181
Size: 1288 75 714 104
Fragments arranged by size:
1288 714 104 75
SphI G_CATG'C
Cuts at: 0 1653 2181
Size: 1653 528
StuI AGG'CCT
Cuts at: 0 1414 1441 2181
Size: 1414 27 740
Fragments arranged by size:
1414 740 27
StyI C'CwwG_G
Cuts at: 0 473 533 999 1436 2181
Size: 473 60 466 437 745
Fragments arranged by size:
745 473 466 437 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 955 959 2181
Size: 136 819 4 1222
Fragments arranged by size:
1222 819 136 4
TatI w'GTAC_w
Cuts at: 0 1298 1775 2181
Size: 1298 477 406
Fragments arranged by size:
1298 477 406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2037 2069 2181
Size: 2037 32 112
Fragments arranged by size:
2037 112 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 997 1617 1701 2181
Size: 997 620 84 480
Fragments arranged by size:
997 620 480 84
XhoI C'TCGA_G
Cuts at: 0 1363 2181
Size: 1363 818
XmaI C'CCGG_G
Cuts at: 0 1613 2181
Size: 1613 568
ZraI GAC'GTC
Cuts at: 0 594 801 1833 2181
Size: 594 207 1032 348
Fragments arranged by size:
1032 594 348 207
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AleI AloI AlwNI
ApaI AvaI BanI BanII BbsI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI BtgI ClaI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII KpnI MmeI MscI MslI MspA1I NcoI NspI
PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SapI SexAI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BfrBI BmtI Bpu10I BsaAI BsaBI
BsiEI BsiWI BsmI BspEI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FseI HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PsiI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XmnI