Restriction map for
pF1KA0713
(Linear) (Six-base) MAPSORT of: KIAA0713.seq Check: 7221 from: 1 to: 2871
KIAA0713.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AccI GT'mk_AC
Cuts at: 0 1136 2871
Size: 1136 1735
AclI AA'CG_TT
Cuts at: 0 2505 2871
Size: 2505 366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1491 2653 2871
Size: 1491 1162 218
Fragments arranged by size:
1491 1162 218
AflIII A'CryG_T
Cuts at: 0 1550 2871
Size: 1550 1321
AlwNI CAG_nnn'CTG
Cuts at: 0 1324 1956 2260 2548 2871
Size: 1324 632 304 288 323
Fragments arranged by size:
1324 632 323 304 288
ApaLI G'TGCA_C
Cuts at: 0 639 2871
Size: 639 2232
ApoI r'AATT_y
Cuts at: 0 770 1331 1629 2770 2871
Size: 770 561 298 1141 101
Fragments arranged by size:
1141 770 561 298 101
AseI AT'TA_AT
Cuts at: 0 784 815 2438 2871
Size: 784 31 1623 433
Fragments arranged by size:
1623 784 433 31
BamHI G'GATC_C
Cuts at: 0 1576 2871
Size: 1576 1295
BanI G'GyrC_C
Cuts at: 0 286 2226 2871
Size: 286 1940 645
Fragments arranged by size:
1940 645 286
BbsI GAAGACnn'nnnn_
Cuts at: 0 2721 2871
Size: 2721 150
BciVI GTATCCnnnnn_n'
Cuts at: 0 1216 2871
Size: 1216 1655
BclI T'GATC_A
Cuts at: 0 549 1716 2871
Size: 549 1167 1155
Fragments arranged by size:
1167 1155 549
BfrBI ATG'CAT
Cuts at: 0 1735 1942 2518 2871
Size: 1735 207 576 353
Fragments arranged by size:
1735 576 353 207
BglII A'GATC_T
Cuts at: 0 573 952 2871
Size: 573 379 1919
Fragments arranged by size:
1919 573 379
BlpI GC'TnA_GC
Cuts at: 0 1541 2871
Size: 1541 1330
Bme1580I G_kGCm'C
Cuts at: 0 643 2871
Size: 643 2228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 734 1202 2871
Size: 295 439 468 1669
Fragments arranged by size:
1669 468 439 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1020 1842 2038 2831 2871
Size: 354 666 822 196 793 40
Fragments arranged by size:
822 793 666 354 196 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 1616 2871
Size: 1616 1255
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1792 2871
Size: 1792 1079
BseYI C'CCAG_C
Cuts at: 0 1986 2871
Size: 1986 885
BsiHKAI G_wGCw'C
Cuts at: 0 643 2871
Size: 643 2228
BsmI GAATG_Cn'
Cuts at: 0 1127 1521 2796 2871
Size: 1127 394 1275 75
Fragments arranged by size:
1275 1127 394 75
Bsp1286I G_dGCh'C
Cuts at: 0 643 2871
Size: 643 2228
BspHI T'CATG_A
Cuts at: 0 1419 2871
Size: 1419 1452
BsrFI r'CCGG_y
Cuts at: 0 1757 2871
Size: 1757 1114
BsrGI T'GTAC_A
Cuts at: 0 1752 2871
Size: 1752 1119
BssSI C'ACGA_G
Cuts at: 0 764 2871
Size: 764 2107
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1741 2871
Size: 1741 1130
BstBI TT'CG_AA
Cuts at: 0 2574 2871
Size: 2574 297
BstEII G'GTnAC_C
Cuts at: 0 1230 2871
Size: 1230 1641
BstYI r'GATC_y
Cuts at: 0 573 952 1425 1560 1576 1932 2871
Size: 573 379 473 135 16 356 939
Fragments arranged by size:
939 573 473 379 356 135 16
Bsu36I CC'TnA_GG
Cuts at: 0 670 2871
Size: 670 2201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 386 2251 2871
Size: 386 1865 620
Fragments arranged by size:
1865 620 386
DraIII CAC_nnn'GTG
Cuts at: 0 2004 2871
Size: 2004 867
DrdI GACnn_nn'nnGTC
Cuts at: 0 2160 2871
Size: 2160 711
EaeI y'GGCC_r
Cuts at: 0 2524 2871
Size: 2524 347
EarI CTCTTCn'nnn_
Cuts at: 0 413 2871
Size: 413 2458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 734 1202 1491 2653 2871
Size: 295 439 468 289 1162 218
Fragments arranged by size:
1162 468 439 295 289 218
EcoRI G'AATT_C
Cuts at: 0 770 2871
Size: 770 2101
EcoRV GAT'ATC
Cuts at: 0 978 2871
Size: 978 1893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2689 2871
Size: 2689 182
FspI TGC'GCA
Cuts at: 0 1531 2871
Size: 1531 1340
FspAI rTGC'GCAy
Cuts at: 0 1531 2871
Size: 1531 1340
HaeII r_GCGC'y
Cuts at: 0 2854 2871
Size: 2854 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 731 763 1582 1614 2871
Size: 364 32 335 32 819 32 1257
Fragments arranged by size:
1257 819 364 335 32 32 32
HincII GTy'rAC
Cuts at: 0 5 2454 2463 2871
Size: 5 2449 9 408
Fragments arranged by size:
2449 408 9 5
HpaI GTT'AAC
Cuts at: 0 5 2871
Size: 5 2866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1308 1453 2871
Size: 229 1079 145 1418
Fragments arranged by size:
1418 1079 229 145
MscI TGG'CCA
Cuts at: 0 2526 2871
Size: 2526 345
MslI CAynn'nnrTG
Cuts at: 0 832 2871
Size: 832 2039
MspA1I CmG'CkG
Cuts at: 0 967 1321 2871
Size: 967 354 1550
Fragments arranged by size:
1550 967 354
NdeI CA'TA_TG
Cuts at: 0 25 829 1731 2424 2871
Size: 25 804 902 693 447
Fragments arranged by size:
902 804 693 447 25
NsiI A_TGCA'T
Cuts at: 0 1737 1944 2520 2871
Size: 1737 207 576 351
Fragments arranged by size:
1737 576 351 207
NspI r_CATG'y
Cuts at: 0 1173 1536 1554 2871
Size: 1173 363 18 1317
Fragments arranged by size:
1317 1173 363 18
PciI A'CATG_T
Cuts at: 0 1550 2871
Size: 1550 1321
PfoI T'CCnGG_A
Cuts at: 0 711 1875 2871
Size: 711 1164 996
Fragments arranged by size:
1164 996 711
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2014 2046 2871
Size: 2014 32 825
Fragments arranged by size:
2014 825 32
PvuII CAG'CTG
Cuts at: 0 967 1321 2871
Size: 967 354 1550
Fragments arranged by size:
1550 967 354
SfcI C'TryA_G
Cuts at: 0 1616 2031 2871
Size: 1616 415 840
Fragments arranged by size:
1616 840 415
SgrAI Cr'CCGG_yG
Cuts at: 0 1757 2871
Size: 1757 1114
SmlI C'TyrA_G
Cuts at: 0 333 1035 1857 2017 2846 2871
Size: 333 702 822 160 829 25
Fragments arranged by size:
829 822 702 333 160 25
SspI AAT'ATT
Cuts at: 0 86 897 2310 2871
Size: 86 811 1413 561
Fragments arranged by size:
1413 811 561 86
StuI AGG'CCT
Cuts at: 0 865 2871
Size: 865 2006
StyI C'CwwG_G
Cuts at: 0 42 2871
Size: 42 2829
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 2871
Size: 109 2762
TatI w'GTAC_w
Cuts at: 0 1674 1752 2871
Size: 1674 78 1119
Fragments arranged by size:
1674 1119 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 825 857 2871
Size: 825 32 2014
Fragments arranged by size:
2014 825 32
XbaI T'CTAG_A
Cuts at: 0 957 2871
Size: 957 1914
XmnI GAAnn'nnTTC
Cuts at: 0 383 2506 2690 2871
Size: 383 2123 184 181
Fragments arranged by size:
2123 383 184 181
Enzymes that do cut:
AccI AclI AcuI AflIII AlwNI ApaLI ApoI AseI
BamHI BanI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BpmI BpuEI BsaI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgZI DraIII DrdI EaeI EarI Eco57MI
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HpaI MmeI MscI MslI MspA1I NdeI NsiI NspI
PciI PfoI PsrI PvuII SfcI SgrAI SmlI SspI
StuI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI
BanII BbeI BbvCI BcgI BglI BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BssHII
BstXI BstZ17I BtgI BtsI ClaI CspCI DraI EagI
EciI EcoICRI EcoNI EcoO109I FseI HindIII KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XcmI XhoI XmaI ZraI