Restriction map for
pF1KA0691
(Linear) (Six-base) MAPSORT of: KIAA0691.seq Check: 6504 from: 1 to: 2259
KIAA0691.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 402 2259
Size: 402 1857
AccI GT'mk_AC
Cuts at: 0 1999 2259
Size: 1999 260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1518 2259
Size: 866 652 741
Fragments arranged by size:
866 741 652
AlwNI CAG_nnn'CTG
Cuts at: 0 711 1995 2055 2259
Size: 711 1284 60 204
Fragments arranged by size:
1284 711 204 60
ApaI G_GGCC'C
Cuts at: 0 1063 1148 1369 1810 2259
Size: 1063 85 221 441 449
Fragments arranged by size:
1063 449 441 221 85
ApoI r'AATT_y
Cuts at: 0 1981 2259
Size: 1981 278
AvaI C'yCGr_G
Cuts at: 0 691 1092 2259
Size: 691 401 1167
Fragments arranged by size:
1167 691 401
BanI G'GyrC_C
Cuts at: 0 1096 1273 1491 1555 1865 2164 2259
Size: 1096 177 218 64 310 299 95
Fragments arranged by size:
1096 310 299 218 177 95 64
BanII G_rGCy'C
Cuts at: 0 1063 1148 1369 1624 1810 2135 2259
Size: 1063 85 221 255 186 325 124
Fragments arranged by size:
1063 325 255 221 186 124 85
BbeI G_GCGC'C
Cuts at: 0 1277 2259
Size: 1277 982
BbvCI CC'TCA_GC
Cuts at: 0 172 1740 2259
Size: 172 1568 519
Fragments arranged by size:
1568 519 172
BglII A'GATC_T
Cuts at: 0 755 2259
Size: 755 1504
Bme1580I G_kGCm'C
Cuts at: 0 1056 1063 1099 1148 1369 1560 1810 1817
Size: 1056 7 36 49 221 191 250 7
Cuts at: 1817 2041 2167 2259
Size: 224 126 92
Fragments arranged by size:
1056 250 224 221 191 126 92 49
36 7 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1133 1409 1813 2259
Size: 1133 276 404 446
Fragments arranged by size:
1133 446 404 276
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1025 2259
Size: 1025 1234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 881 2053 2239 2259
Size: 780 101 1172 186 20
Fragments arranged by size:
1172 780 186 101 20
Bpu10I CC'TnA_GC
Cuts at: 0 172 1458 1610 1714 1740 2259
Size: 172 1286 152 104 26 519
Fragments arranged by size:
1286 519 172 152 104 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1020 2259
Size: 28 992 1239
Fragments arranged by size:
1239 992 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 283 544 2259
Size: 283 261 1715
Fragments arranged by size:
1715 283 261
BsaAI yAC'GTr
Cuts at: 0 537 2259
Size: 537 1722
BsaHI Gr'CG_yC
Cuts at: 0 593 1274 1933 2259
Size: 593 681 659 326
Fragments arranged by size:
681 659 593 326
BsaWI w'CCGG_w
Cuts at: 0 491 2259
Size: 491 1768
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 562 592 652 682 1089 1119
Size: 430 30 102 30 60 30 407 30
Cuts at: 1119 1469 1499 2259
Size: 350 30 760
Fragments arranged by size:
760 430 407 350 102 60 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 2143 2259
Size: 673 1470 116
Fragments arranged by size:
1470 673 116
BseYI C'CCAG_C
Cuts at: 0 730 814 819 948 1081 1147 1157 1171
Size: 730 84 5 129 133 66 10 14
Cuts at: 1171 1396 1438 1480 1513 1528 1745 1788 2259
Size: 225 42 42 33 15 217 43 471
Fragments arranged by size:
730 471 225 217 133 129 84 66
43 42 42 33 15 14 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1672 2259
Size: 472 1200 587
Fragments arranged by size:
1200 587 472
BsiEI CG_ry'CG
Cuts at: 0 2003 2259
Size: 2003 256
BsiHKAI G_wGCw'C
Cuts at: 0 1006 1624 2259
Size: 1006 618 635
Fragments arranged by size:
1006 635 618
BsmBI CGTCTCn'nnnn_
Cuts at: 0 247 2259
Size: 247 2012
Bsp1286I G_dGCh'C
Cuts at: 0 1006 1056 1063 1099 1148 1369 1560 1624
Size: 1006 50 7 36 49 221 191 64
Cuts at: 1624 1810 1817 2041 2135 2167 2259
Size: 186 7 224 94 32 92
Fragments arranged by size:
1006 224 221 191 186 94 92 64
50 49 36 32 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 2259
Size: 402 1857
BsrFI r'CCGG_y
Cuts at: 0 1246 1552 2259
Size: 1246 306 707
Fragments arranged by size:
1246 707 306
BssSI C'ACGA_G
Cuts at: 0 2125 2259
Size: 2125 134
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1555 1872 2259
Size: 1555 317 387
Fragments arranged by size:
1555 387 317
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1488 2259
Size: 1488 771
BstYI r'GATC_y
Cuts at: 0 342 755 2259
Size: 342 413 1504
Fragments arranged by size:
1504 413 342
BtgI C'CryG_G
Cuts at: 0 833 1634 1847 2259
Size: 833 801 213 412
Fragments arranged by size:
833 801 412 213
BtsI GCAGTG_nn'
Cuts at: 0 1248 1338 1495 2259
Size: 1248 90 157 764
Fragments arranged by size:
1248 764 157 90
DrdI GACnn_nn'nnGTC
Cuts at: 0 883 2259
Size: 883 1376
EaeI y'GGCC_r
Cuts at: 0 372 2259
Size: 372 1887
EarI CTCTTCn'nnn_
Cuts at: 0 359 853 1847 2017 2259
Size: 359 494 994 170 242
Fragments arranged by size:
994 494 359 242 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1208 1229 1654 2259
Size: 19 1189 21 425 605
Fragments arranged by size:
1189 605 425 21 19
EcoICRI GAG'CTC
Cuts at: 0 1622 2259
Size: 1622 637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 866 881 1518 2053 2239 2259
Size: 780 86 15 637 535 186 20
Fragments arranged by size:
780 637 535 186 86 20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 145 1187 1822 2259
Size: 46 99 1042 635 437
Fragments arranged by size:
1042 635 437 99 46
EcoO109I rG'GnC_Cy
Cuts at: 0 318 645 1026 1060 1366 1477 1605 1671
Size: 318 327 381 34 306 111 128 66
Cuts at: 1671 1807 2067 2250 2259
Size: 136 260 183 9
Fragments arranged by size:
381 327 318 306 260 183 136 128
111 66 34 9
EcoRI G'AATT_C
Cuts at: 0 1981 2259
Size: 1981 278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2232 2259
Size: 2232 27
FspI TGC'GCA
Cuts at: 0 541 562 2259
Size: 541 21 1697
Fragments arranged by size:
1697 541 21
FspAI rTGC'GCAy
Cuts at: 0 541 2259
Size: 541 1718
HaeII r_GCGC'y
Cuts at: 0 712 1277 1995 2259
Size: 712 565 718 264
Fragments arranged by size:
718 712 565 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 562 594 1469 1501 1694 1726
Size: 430 32 100 32 875 32 193 32
Cuts at: 1726 2259
Size: 533
Fragments arranged by size:
875 533 430 193 100 32 32 32
32
HincII GTy'rAC
Cuts at: 0 591 633 2000 2259
Size: 591 42 1367 259
Fragments arranged by size:
1367 591 259 42
HindIII A'AGCT_T
Cuts at: 0 302 2259
Size: 302 1957
KasI G'GCGC_C
Cuts at: 0 1273 2259
Size: 1273 986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1954 2259
Size: 227 1727 305
Fragments arranged by size:
1727 305 227
MscI TGG'CCA
Cuts at: 0 374 2259
Size: 374 1885
MslI CAynn'nnrTG
Cuts at: 0 412 2259
Size: 412 1847
MspA1I CmG'CkG
Cuts at: 0 952 1208 1761 1782 2202 2259
Size: 952 256 553 21 420 57
Fragments arranged by size:
952 553 420 256 57 21
NarI GG'CG_CC
Cuts at: 0 1274 2259
Size: 1274 985
NcoI C'CATG_G
Cuts at: 0 1634 1847 2259
Size: 1634 213 412
Fragments arranged by size:
1634 412 213
NspI r_CATG'y
Cuts at: 0 411 546 567 1875 2259
Size: 411 135 21 1308 384
Fragments arranged by size:
1308 411 384 135 21
PasI CC'CwG_GG
Cuts at: 0 1056 2259
Size: 1056 1203
PflMI CCAn_nnn'nTGG
Cuts at: 0 1122 1362 2259
Size: 1122 240 897
Fragments arranged by size:
1122 897 240
PmlI CAC'GTG
Cuts at: 0 537 2259
Size: 537 1722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 884 2259
Size: 852 32 1375
Fragments arranged by size:
1375 852 32
PpuMI rG'GwC_Cy
Cuts at: 0 645 1026 1477 1671 2250 2259
Size: 645 381 451 194 579 9
Fragments arranged by size:
645 579 451 381 194 9
PspOMI G'GGCC_C
Cuts at: 0 1059 1144 1365 1806 2259
Size: 1059 85 221 441 453
Fragments arranged by size:
1059 453 441 221 85
PspXI vC'TCGA_Gb
Cuts at: 0 691 2259
Size: 691 1568
PstI C_TGCA'G
Cuts at: 0 1760 2259
Size: 1760 499
PvuII CAG'CTG
Cuts at: 0 952 1761 2259
Size: 952 809 498
Fragments arranged by size:
952 809 498
SacI G_AGCT'C
Cuts at: 0 1624 2259
Size: 1624 635
SalI G'TCGA_C
Cuts at: 0 1998 2259
Size: 1998 261
SanDI GG'GwC_CC
Cuts at: 0 1026 1477 2259
Size: 1026 451 782
Fragments arranged by size:
1026 782 451
ScaI AGT'ACT
Cuts at: 0 625 2259
Size: 625 1634
SfcI C'TryA_G
Cuts at: 0 1756 2259
Size: 1756 503
SfoI GGC'GCC
Cuts at: 0 1275 2259
Size: 1275 984
SmlI C'TyrA_G
Cuts at: 0 43 691 999 2259
Size: 43 648 308 1260
Fragments arranged by size:
1260 648 308 43
SphI G_CATG'C
Cuts at: 0 546 567 2259
Size: 546 21 1692
Fragments arranged by size:
1692 546 21
StuI AGG'CCT
Cuts at: 0 1360 2259
Size: 1360 899
StyI C'CwwG_G
Cuts at: 0 21 1064 1361 1544 1634 1823 1847 2063
Size: 21 1043 297 183 90 189 24 216
Cuts at: 2063 2259
Size: 196
Fragments arranged by size:
1043 297 216 196 189 183 90 24
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1711 1780 2017 2259
Size: 1711 69 237 242
Fragments arranged by size:
1711 242 237 69
TatI w'GTAC_w
Cuts at: 0 623 1720 2102 2259
Size: 623 1097 382 157
Fragments arranged by size:
1097 623 382 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 928 960 1089 1121 2259
Size: 928 32 129 32 1138
Fragments arranged by size:
1138 928 129 32 32
XhoI C'TCGA_G
Cuts at: 0 691 2259
Size: 691 1568
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbvCI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NarI NcoI NspI PasI PflMI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI
SalI SanDI ScaI SfcI SfoI SmlI SphI StuI
StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BsaBI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EcoRV FseI
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PshAI
PsiI PsrI PvuI RsrII SacII SapI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XcmI XmaI XmnI ZraI