Restriction map for
pF1KSDA0690
(Linear) (Six-base) MAPSORT of: KIAA0690.seq Check: 252 from: 1 to: 3591
KIAA0690.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 3585 3591
Size: 3585 6
Acc65I G'GTAC_C
Cuts at: 0 277 3591
Size: 277 3314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 303 652 1048 1507 1760 2170 3591
Size: 108 195 349 396 459 253 410 1421
Fragments arranged by size:
1421 459 410 396 349 253 195 108
AfeI AGC'GCT
Cuts at: 0 49 334 3591
Size: 49 285 3257
Fragments arranged by size:
3257 285 49
AflII C'TTAA_G
Cuts at: 0 871 3591
Size: 871 2720
AflIII A'CryG_T
Cuts at: 0 2640 3591
Size: 2640 951
AleI CACnn'nnGTG
Cuts at: 0 397 1554 3591
Size: 397 1157 2037
Fragments arranged by size:
2037 1157 397
AlwNI CAG_nnn'CTG
Cuts at: 0 1090 1179 1245 1692 1729 2529 2611 3591
Size: 1090 89 66 447 37 800 82 980
Fragments arranged by size:
1090 980 800 447 89 82 66 37
ApaI G_GGCC'C
Cuts at: 0 2113 2121 3591
Size: 2113 8 1470
Fragments arranged by size:
2113 1470 8
ApaLI G'TGCA_C
Cuts at: 0 2292 3591
Size: 2292 1299
ApoI r'AATT_y
Cuts at: 0 502 1144 3591
Size: 502 642 2447
Fragments arranged by size:
2447 642 502
AvaI C'yCGr_G
Cuts at: 0 9 273 2113 2773 2819 3591
Size: 9 264 1840 660 46 772
Fragments arranged by size:
1840 772 660 264 46 9
BamHI G'GATC_C
Cuts at: 0 3063 3444 3591
Size: 3063 381 147
Fragments arranged by size:
3063 381 147
BanI G'GyrC_C
Cuts at: 0 277 2831 3208 3591
Size: 277 2554 377 383
Fragments arranged by size:
2554 383 377 277
BanII G_rGCy'C
Cuts at: 0 797 1165 2113 2121 2841 2894 3050 3591
Size: 797 368 948 8 720 53 156 541
Fragments arranged by size:
948 797 720 541 368 156 53 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1179 2161 3591
Size: 1179 982 1430
Fragments arranged by size:
1430 1179 982
BbvCI CC'TCA_GC
Cuts at: 0 2813 3302 3591
Size: 2813 489 289
Fragments arranged by size:
2813 489 289
BclI T'GATC_A
Cuts at: 0 3161 3254 3591
Size: 3161 93 337
Fragments arranged by size:
3161 337 93
BglI GCCn_nnn'nGGC
Cuts at: 0 739 2432 3376 3591
Size: 739 1693 944 215
Fragments arranged by size:
1693 944 739 215
BglII A'GATC_T
Cuts at: 0 368 1538 3591
Size: 368 1170 2053
Fragments arranged by size:
2053 1170 368
Bme1580I G_kGCm'C
Cuts at: 0 2113 2121 2296 3591
Size: 2113 8 175 1295
Fragments arranged by size:
2113 1295 175 8
BmgBI CAC'GTC
Cuts at: 0 2571 2622 3591
Size: 2571 51 969
Fragments arranged by size:
2571 969 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 830 2596 3591
Size: 830 1766 995
Fragments arranged by size:
1766 995 830
BmtI G_CTAG'C
Cuts at: 0 865 3591
Size: 865 2726
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 288 366 3591
Size: 288 78 3225
Fragments arranged by size:
3225 288 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1179 1222 1537 1802 2104 2161 2551
Size: 605 574 43 315 265 302 57 390
Cuts at: 2551 3337 3362 3591
Size: 786 25 229
Fragments arranged by size:
786 605 574 390 315 302 265 229
57 43 25
Bpu10I CC'TnA_GC
Cuts at: 0 775 1511 2813 2842 3302 3591
Size: 775 736 1302 29 460 289
Fragments arranged by size:
1302 775 736 460 289 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 726 1000 3268 3591
Size: 475 251 274 2268 323
Fragments arranged by size:
2268 475 323 274 251
BsaI GGTCTCn'nnnn_
Cuts at: 0 1415 3591
Size: 1415 2176
BsaHI Gr'CG_yC
Cuts at: 0 229 3591
Size: 229 3362
BsaWI w'CCGG_w
Cuts at: 0 455 2804 3591
Size: 455 2349 787
Fragments arranged by size:
2349 787 455
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 1048 1078 2077 2107 2524 2554
Size: 421 30 597 30 999 30 417 30
Cuts at: 2554 3591
Size: 1037
Fragments arranged by size:
1037 999 597 421 417 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 679 1065 1141 1390 2101 2175 2791 2875
Size: 679 386 76 249 711 74 616 84
Cuts at: 2875 2887 2890 2893 2896 2899 2902 2935 2962
Size: 12 3 3 3 3 3 33 27
Cuts at: 2962 2974 2977 3024 3187 3319 3322 3325 3591
Size: 12 3 47 163 132 3 3 266
Fragments arranged by size:
711 679 616 386 266 249 163 132
84 76 74 47 33 27 12 12
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 467 1638 3591
Size: 467 1171 1953
Fragments arranged by size:
1953 1171 467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 598 1572 2080 2149 2567 2665 3591
Size: 288 310 974 508 69 418 98 926
Fragments arranged by size:
974 926 508 418 310 288 98 69
BsiEI CG_ry'CG
Cuts at: 0 133 3442 3591
Size: 133 3309 149
Fragments arranged by size:
3309 149 133
BsiHKAI G_wGCw'C
Cuts at: 0 781 797 1165 1922 2182 2296 3591
Size: 781 16 368 757 260 114 1295
Fragments arranged by size:
1295 781 757 368 260 114 16
BsmI GAATG_Cn'
Cuts at: 0 1039 3353 3591
Size: 1039 2314 238
Fragments arranged by size:
2314 1039 238
BsmBI CGTCTCn'nnnn_
Cuts at: 0 220 418 3591
Size: 220 198 3173
Fragments arranged by size:
3173 220 198
Bsp1286I G_dGCh'C
Cuts at: 0 781 797 1165 1922 2113 2121 2182 2296
Size: 781 16 368 757 191 8 61 114
Cuts at: 2296 2841 2894 3050 3591
Size: 545 53 156 541
Fragments arranged by size:
781 757 545 541 368 191 156 114
61 53 16 8
BspEI T'CCGG_A
Cuts at: 0 455 2804 3591
Size: 455 2349 787
Fragments arranged by size:
2349 787 455
BspHI T'CATG_A
Cuts at: 0 698 3591
Size: 698 2893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 647 973 1262 3591
Size: 647 326 289 2329
Fragments arranged by size:
2329 647 326 289
BsrFI r'CCGG_y
Cuts at: 0 128 3155 3437 3591
Size: 128 3027 282 154
Fragments arranged by size:
3027 282 154 128
BssSI C'ACGA_G
Cuts at: 0 2341 3591
Size: 2341 1250
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1350 3591
Size: 1350 2241
BstEII G'GTnAC_C
Cuts at: 0 960 1314 1674 2439 3591
Size: 960 354 360 765 1152
Fragments arranged by size:
1152 960 765 360 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1400 1506 2517 3591
Size: 1400 106 1011 1074
Fragments arranged by size:
1400 1074 1011 106
BstYI r'GATC_y
Cuts at: 0 368 1022 1538 3063 3444 3591
Size: 368 654 516 1525 381 147
Fragments arranged by size:
1525 654 516 381 368 147
Bsu36I CC'TnA_GG
Cuts at: 0 691 1083 1331 1669 2105 2365 3591
Size: 691 392 248 338 436 260 1226
Fragments arranged by size:
1226 691 436 392 338 260 248
BtgI C'CryG_G
Cuts at: 0 233 1393 1499 1799 2052 2444 2855 3591
Size: 233 1160 106 300 253 392 411 736
Fragments arranged by size:
1160 736 411 392 300 253 233 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3343 3591
Size: 3343 248
BtsI GCAGTG_nn'
Cuts at: 0 504 548 684 919 1127 1307 2158 2264
Size: 504 44 136 235 208 180 851 106
Cuts at: 2264 2335 2404 2612 3591
Size: 71 69 208 979
Fragments arranged by size:
979 851 504 235 208 208 180 136
106 71 69 44
DraI TTT'AAA
Cuts at: 0 2493 3591
Size: 2493 1098
DraIII CAC_nnn'GTG
Cuts at: 0 1221 1682 2058 2229 3591
Size: 1221 461 376 171 1362
Fragments arranged by size:
1362 1221 461 376 171
DrdI GACnn_nn'nnGTC
Cuts at: 0 154 1672 3591
Size: 154 1518 1919
Fragments arranged by size:
1919 1518 154
EaeI y'GGCC_r
Cuts at: 0 130 1607 1646 2633 3089 3153 3368 3439
Size: 130 1477 39 987 456 64 215 71
Cuts at: 3439 3591
Size: 152
Fragments arranged by size:
1477 987 456 215 152 130 71 64
39
EagI C'GGCC_G
Cuts at: 0 130 3439 3591
Size: 130 3309 152
Fragments arranged by size:
3309 152 130
EarI CTCTTCn'nnn_
Cuts at: 0 236 664 760 1483 1783 2128 2381 2864
Size: 236 428 96 723 300 345 253 483
Cuts at: 2864 3194 3221 3591
Size: 330 27 370
Fragments arranged by size:
723 483 428 370 345 330 300 253
236 96 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 262 3591
Size: 262 3329
EcoICRI GAG'CTC
Cuts at: 0 795 1163 3591
Size: 795 368 2428
Fragments arranged by size:
2428 795 368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 303 605 652 1048 1179 1222 1507
Size: 108 195 302 47 396 131 43 285
Cuts at: 1507 1537 1760 1802 2104 2161 2170 2551 3337
Size: 30 223 42 302 57 9 381 786
Cuts at: 3337 3362 3591
Size: 25 229
Fragments arranged by size:
786 396 381 302 302 285 229 223
195 131 108 57 47 43 42 30
25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1510 2845 3529 3591
Size: 1510 1335 684 62
Fragments arranged by size:
1510 1335 684 62
EcoO109I rG'GnC_Cy
Cuts at: 0 194 519 636 918 1087 1131 1327 1689
Size: 194 325 117 282 169 44 196 362
Cuts at: 1689 2109 2110 2117 2118 2145 2201 2391 3591
Size: 420 1 7 1 27 56 190 1200
Fragments arranged by size:
1200 420 362 325 282 196 194 190
169 117 56 44 27 7 1 1
EcoRI G'AATT_C
Cuts at: 0 502 3591
Size: 502 3089
FseI GG_CCGG'CC
Cuts at: 0 3441 3591
Size: 3441 150
FspI TGC'GCA
Cuts at: 0 1696 3591
Size: 1696 1895
HaeII r_GCGC'y
Cuts at: 0 51 336 3149 3591
Size: 51 285 2813 442
Fragments arranged by size:
2813 442 285 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 1048 1080 1940 1972 2780 2812
Size: 172 32 844 32 860 32 808 32
Cuts at: 2812 3312 3344 3591
Size: 500 32 247
Fragments arranged by size:
860 844 808 500 247 172 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2799 3586 3591
Size: 2799 787 5
Fragments arranged by size:
2799 787 5
HindIII A'AGCT_T
Cuts at: 0 2671 2707 3591
Size: 2671 36 884
Fragments arranged by size:
2671 884 36
KpnI G_GTAC'C
Cuts at: 0 281 3591
Size: 281 3310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 371 3591
Size: 323 48 3220
Fragments arranged by size:
3220 323 48
MscI TGG'CCA
Cuts at: 0 1609 1648 2635 3091 3370 3591
Size: 1609 39 987 456 279 221
Fragments arranged by size:
1609 987 456 279 221 39
MslI CAynn'nnrTG
Cuts at: 0 397 1554 2599 2620 3591
Size: 397 1157 1045 21 971
Fragments arranged by size:
1157 1045 971 397 21
MspA1I CmG'CkG
Cuts at: 0 1176 2245 2453 2526 2649 2991 3591
Size: 1176 1069 208 73 123 342 600
Fragments arranged by size:
1176 1069 600 342 208 123 73
NaeI GCC'GGC
Cuts at: 0 130 3157 3439 3591
Size: 130 3027 282 152
Fragments arranged by size:
3027 282 152 130
NcoI C'CATG_G
Cuts at: 0 233 1499 2444 3591
Size: 233 1266 945 1147
Fragments arranged by size:
1266 1147 945 233
NgoMIV G'CCGG_C
Cuts at: 0 128 3155 3437 3591
Size: 128 3027 282 154
Fragments arranged by size:
3027 282 154 128
NheI G'CTAG_C
Cuts at: 0 861 3591
Size: 861 2730
NspI r_CATG'y
Cuts at: 0 621 2644 2688 3591
Size: 621 2023 44 903
Fragments arranged by size:
2023 903 621 44
PasI CC'CwG_GG
Cuts at: 0 411 2898 3591
Size: 411 2487 693
Fragments arranged by size:
2487 693 411
PciI A'CATG_T
Cuts at: 0 2640 3591
Size: 2640 951
PflMI CCAn_nnn'nTGG
Cuts at: 0 717 1302 3591
Size: 717 585 2289
Fragments arranged by size:
2289 717 585
PfoI T'CCnGG_A
Cuts at: 0 566 653 1895 1958 3059 3591
Size: 566 87 1242 63 1101 532
Fragments arranged by size:
1242 1101 566 532 87 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1412 1444 3591
Size: 1412 32 2147
Fragments arranged by size:
2147 1412 32
PpuMI rG'GwC_Cy
Cuts at: 0 194 636 918 1327 2145 3591
Size: 194 442 282 409 818 1446
Fragments arranged by size:
1446 818 442 409 282 194
PshAI GACnn'nnGTC
Cuts at: 0 2446 3591
Size: 2446 1145
PspOMI G'GGCC_C
Cuts at: 0 2109 2117 3591
Size: 2109 8 1474
Fragments arranged by size:
2109 1474 8
PstI C_TGCA'G
Cuts at: 0 188 679 1346 2399 2458 3509 3591
Size: 188 491 667 1053 59 1051 82
Fragments arranged by size:
1053 1051 667 491 188 82 59
PvuII CAG'CTG
Cuts at: 0 1176 2245 2453 2526 2649 3591
Size: 1176 1069 208 73 123 942
Fragments arranged by size:
1176 1069 942 208 123 73
SacI G_AGCT'C
Cuts at: 0 797 1165 3591
Size: 797 368 2426
Fragments arranged by size:
2426 797 368
SalI G'TCGA_C
Cuts at: 0 3584 3591
Size: 3584 7
SapI GCTCTTCn'nnn_
Cuts at: 0 664 1483 3591
Size: 664 819 2108
Fragments arranged by size:
2108 819 664
ScaI AGT'ACT
Cuts at: 0 439 3591
Size: 439 3152
SexAI A'CCwGG_T
Cuts at: 0 899 1964 3591
Size: 899 1065 1627
Fragments arranged by size:
1627 1065 899
SfcI C'TryA_G
Cuts at: 0 184 675 1342 1598 2395 2454 3505 3591
Size: 184 491 667 256 797 59 1051 86
Fragments arranged by size:
1051 797 667 491 256 184 86 59
SmaI CCC'GGG
Cuts at: 0 2115 2821 3591
Size: 2115 706 770
Fragments arranged by size:
2115 770 706
SmlI C'TyrA_G
Cuts at: 0 490 705 871 1015 3283 3591
Size: 490 215 166 144 2268 308
Fragments arranged by size:
2268 490 308 215 166 144
SrfI GCCC'GGGC
Cuts at: 0 2821 3591
Size: 2821 770
StuI AGG'CCT
Cuts at: 0 662 742 768 1596 2071 3524 3591
Size: 662 80 26 828 475 1453 67
Fragments arranged by size:
1453 828 662 475 80 67 26
StyI C'CwwG_G
Cuts at: 0 233 593 1499 1709 1976 2297 2444 3068
Size: 233 360 906 210 267 321 147 624
Cuts at: 3068 3591
Size: 523
Fragments arranged by size:
906 624 523 360 321 267 233 210
147
TatI w'GTAC_w
Cuts at: 0 327 437 2512 3591
Size: 327 110 2075 1079
Fragments arranged by size:
2075 1079 327 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 944 976 1319 1351 2077 2109 2524 2556
Size: 944 32 343 32 726 32 415 32
Cuts at: 2556 3591
Size: 1035
Fragments arranged by size:
1035 944 726 415 343 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1966 2572 3591
Size: 1966 606 1019
Fragments arranged by size:
1966 1019 606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 766 3591
Size: 766 2825
XmaI C'CCGG_G
Cuts at: 0 2113 2819 3591
Size: 2113 706 772
Fragments arranged by size:
2113 772 706
XmnI GAAnn'nnTTC
Cuts at: 0 2719 3591
Size: 2719 872
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AleI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BclI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FseI FspI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SalI SapI ScaI SexAI
SfcI SmaI SmlI SrfI StuI StyI TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AlfI AloI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI
BlpI BsaAI BsaBI BsiWI BsrBI BsrDI BsrGI BssHII
BstBI BstZ17I ClaI CspCI EcoRV FalI FspAI HpaI
KasI MfeI MluI NarI NdeI NotI NruI NsiI
PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI TaqII XbaI XhoI ZraI