Restriction map for
pF1KSDA0688
(Linear) (Six-base) MAPSORT of: KIAA0688.seq Check: 9403 from: 1 to: 2511
KIAA0688.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1873 2511
Size: 1873 638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2058 2206 2511
Size: 2058 148 305
Fragments arranged by size:
2058 305 148
AfeI AGC'GCT
Cuts at: 0 634 718 2511
Size: 634 84 1793
Fragments arranged by size:
1793 634 84
AleI CACnn'nnGTG
Cuts at: 0 1834 2248 2511
Size: 1834 414 263
Fragments arranged by size:
1834 414 263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 229 1515 1778 2511
Size: 229 1286 263 733
Fragments arranged by size:
1286 733 263 229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 467 499 1914 1946 2511
Size: 467 32 1415 32 565
Fragments arranged by size:
1415 565 467 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 333 370 492 864 875 960 2472 2511
Size: 333 37 122 372 11 85 1512 39
Fragments arranged by size:
1512 372 333 122 85 39 37 11
ApaI G_GGCC'C
Cuts at: 0 836 848 1364 1490 1808 2511
Size: 836 12 516 126 318 703
Fragments arranged by size:
836 703 516 318 126 12
ApoI r'AATT_y
Cuts at: 0 2080 2511
Size: 2080 431
AvaI C'yCGr_G
Cuts at: 0 22 167 222 1631 1874 2511
Size: 22 145 55 1409 243 637
Fragments arranged by size:
1409 637 243 145 55 22
BamHI G'GATC_C
Cuts at: 0 1194 2511
Size: 1194 1317
BanI G'GyrC_C
Cuts at: 0 226 342 373 388 502 1006 2489 2511
Size: 226 116 31 15 114 504 1483 22
Fragments arranged by size:
1483 504 226 116 114 31 22 15
BanII G_rGCy'C
Cuts at: 0 60 444 534 836 848 1058 1208 1364
Size: 60 384 90 302 12 210 150 156
Cuts at: 1364 1490 1808 2511
Size: 126 318 703
Fragments arranged by size:
703 384 318 302 210 156 150 126
90 60 12
BbeI G_GCGC'C
Cuts at: 0 346 2493 2511
Size: 346 2147 18
Fragments arranged by size:
2147 346 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1089 2511
Size: 1089 1422
BbvCI CC'TCA_GC
Cuts at: 0 141 2511
Size: 141 2370
BciVI GTATCCnnnnn_n'
Cuts at: 0 2086 2511
Size: 2086 425
BfrBI ATG'CAT
Cuts at: 0 1130 1970 2511
Size: 1130 840 541
Fragments arranged by size:
1130 840 541
BglI GCCn_nnn'nGGC
Cuts at: 0 151 1727 2511
Size: 151 1576 784
Fragments arranged by size:
1576 784 151
BlpI GC'TnA_GC
Cuts at: 0 1731 2511
Size: 1731 780
Bme1580I G_kGCm'C
Cuts at: 0 229 836 848 1009 1227 1265 1364 1490
Size: 229 607 12 161 218 38 99 126
Cuts at: 1490 1808 1881 2408 2511
Size: 318 73 527 103
Fragments arranged by size:
607 527 318 229 218 161 126 103
99 73 38 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 438 842 1096 1824 1891 2511
Size: 438 404 254 728 67 620
Fragments arranged by size:
728 620 438 404 254 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 1175 2511
Size: 312 863 1336
Fragments arranged by size:
1336 863 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 469 514 1042 1519 1921 2494 2511
Size: 307 162 45 528 477 402 573 17
Fragments arranged by size:
573 528 477 402 307 162 45 17
Bpu10I CC'TnA_GC
Cuts at: 0 141 347 2511
Size: 141 206 2164
Fragments arranged by size:
2164 206 141
BsaHI Gr'CG_yC
Cuts at: 0 343 2490 2511
Size: 343 2147 21
Fragments arranged by size:
2147 343 21
BsaWI w'CCGG_w
Cuts at: 0 298 405 2165 2511
Size: 298 107 1760 346
Fragments arranged by size:
1760 346 298 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 53 83 169 199 467 497 1195 1225
Size: 53 30 86 30 268 30 698 30
Cuts at: 1225 2511
Size: 1286
Fragments arranged by size:
1286 698 268 86 53 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 190 584 841 1216 1617 1765 1935
Size: 187 3 394 257 375 401 148 170
Cuts at: 1935 2511
Size: 576
Fragments arranged by size:
576 401 394 375 257 187 170 148
3
BseYI C'CCAG_C
Cuts at: 0 354 517 607 987 1565 2333 2511
Size: 354 163 90 380 578 768 178
Fragments arranged by size:
768 578 380 354 178 163 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 346 409 1623 2437 2511
Size: 325 21 63 1214 814 74
Fragments arranged by size:
1214 814 325 74 63 21
BsiEI CG_ry'CG
Cuts at: 0 2413 2511
Size: 2413 98
BsiHKAI G_wGCw'C
Cuts at: 0 1156 1417 2466 2511
Size: 1156 261 1049 45
Fragments arranged by size:
1156 1049 261 45
BsmI GAATG_Cn'
Cuts at: 0 694 2511
Size: 694 1817
BsmBI CGTCTCn'nnnn_
Cuts at: 0 262 2511
Size: 262 2249
Bsp1286I G_dGCh'C
Cuts at: 0 60 229 444 534 836 848 1009 1058
Size: 60 169 215 90 302 12 161 49
Cuts at: 1058 1156 1208 1227 1265 1364 1417 1490 1808
Size: 98 52 19 38 99 53 73 318
Cuts at: 1808 1881 2408 2466 2511
Size: 73 527 58 45
Fragments arranged by size:
527 318 302 215 169 161 99 98
90 73 73 60 58 53 52 49
45 38 19 12
BspEI T'CCGG_A
Cuts at: 0 405 2511
Size: 405 2106
BspHI T'CATG_A
Cuts at: 0 1080 2511
Size: 1080 1431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 297 731 1873 2511
Size: 297 434 1142 638
Fragments arranged by size:
1142 638 434 297
BsrBI CCG'CTC
Cuts at: 0 90 1700 2511
Size: 90 1610 811
Fragments arranged by size:
1610 811 90
BsrDI GCAATG_nn'
Cuts at: 0 1291 2511
Size: 1291 1220
BssSI C'ACGA_G
Cuts at: 0 1641 2511
Size: 1641 870
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1481 2030 2511
Size: 1481 549 481
Fragments arranged by size:
1481 549 481
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1429 2250 2511
Size: 1429 821 261
Fragments arranged by size:
1429 821 261
BstYI r'GATC_y
Cuts at: 0 402 1194 2477 2511
Size: 402 792 1283 34
Fragments arranged by size:
1283 792 402 34
Bsu36I CC'TnA_GG
Cuts at: 0 905 1199 2511
Size: 905 294 1312
Fragments arranged by size:
1312 905 294
BtgI C'CryG_G
Cuts at: 0 699 1582 1808 1906 2117 2319 2511
Size: 699 883 226 98 211 202 192
Fragments arranged by size:
883 699 226 211 202 192 98
BtsI GCAGTG_nn'
Cuts at: 0 1070 1227 1762 2291 2344 2511
Size: 1070 157 535 529 53 167
Fragments arranged by size:
1070 535 529 167 157 53
EaeI y'GGCC_r
Cuts at: 0 152 689 1419 1431 2160 2410 2511
Size: 152 537 730 12 729 250 101
Fragments arranged by size:
730 729 537 250 152 101 12
EagI C'GGCC_G
Cuts at: 0 2410 2511
Size: 2410 101
EarI CTCTTCn'nnn_
Cuts at: 0 544 618 1792 2454 2511
Size: 544 74 1174 662 57
Fragments arranged by size:
1174 662 544 74 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 469 514 1042 1519 1921 2058 2206
Size: 307 162 45 528 477 402 137 148
Cuts at: 2206 2494 2511
Size: 288 17
Fragments arranged by size:
528 477 402 307 288 162 148 137
45 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 2511
Size: 337 2174
EcoO109I rG'GnC_Cy
Cuts at: 0 523 571 832 833 844 891 957 1570
Size: 523 48 261 1 11 47 66 613
Cuts at: 1570 1804 1922 2511
Size: 234 118 589
Fragments arranged by size:
613 589 523 261 234 118 66 48
47 11 1
FspI TGC'GCA
Cuts at: 0 868 2511
Size: 868 1643
HaeII r_GCGC'y
Cuts at: 0 45 346 636 720 2493 2511
Size: 45 301 290 84 1773 18
Fragments arranged by size:
1773 301 290 84 45 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 1195 1227 1930 1962 2296 2328
Size: 394 32 769 32 703 32 334 32
Cuts at: 2328 2511
Size: 183
Fragments arranged by size:
769 703 394 334 183 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 342 2489 2511
Size: 342 2147 22
Fragments arranged by size:
2147 342 22
MscI TGG'CCA
Cuts at: 0 154 1421 1433 2162 2511
Size: 154 1267 12 729 349
Fragments arranged by size:
1267 729 349 154 12
MslI CAynn'nnrTG
Cuts at: 0 431 1427 1834 2230 2248 2511
Size: 431 996 407 396 18 263
Fragments arranged by size:
996 431 407 396 263 18
MspA1I CmG'CkG
Cuts at: 0 563 888 1350 1386 2119 2285 2511
Size: 563 325 462 36 733 166 226
Fragments arranged by size:
733 563 462 325 226 166 36
NarI GG'CG_CC
Cuts at: 0 343 2490 2511
Size: 343 2147 21
Fragments arranged by size:
2147 343 21
NcoI C'CATG_G
Cuts at: 0 1582 1808 2319 2511
Size: 1582 226 511 192
Fragments arranged by size:
1582 511 226 192
NsiI A_TGCA'T
Cuts at: 0 1132 1972 2511
Size: 1132 840 539
Fragments arranged by size:
1132 840 539
NspI r_CATG'y
Cuts at: 0 1107 2511
Size: 1107 1404
PasI CC'CwG_GG
Cuts at: 0 1463 1801 2495 2511
Size: 1463 338 694 16
Fragments arranged by size:
1463 694 338 16
PflMI CCAn_nnn'nTGG
Cuts at: 0 492 1170 1558 1814 2511
Size: 492 678 388 256 697
Fragments arranged by size:
697 678 492 388 256
PfoI T'CCnGG_A
Cuts at: 0 1244 1535 2511
Size: 1244 291 976
Fragments arranged by size:
1244 976 291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 467 499 2511
Size: 467 32 2012
Fragments arranged by size:
2012 467 32
PpuMI rG'GwC_Cy
Cuts at: 0 523 571 957 1570 1922 2511
Size: 523 48 386 613 352 589
Fragments arranged by size:
613 589 523 386 352 48
PspOMI G'GGCC_C
Cuts at: 0 832 844 1360 1486 1804 2511
Size: 832 12 516 126 318 707
Fragments arranged by size:
832 707 516 318 126 12
PstI C_TGCA'G
Cuts at: 0 1222 2298 2511
Size: 1222 1076 213
Fragments arranged by size:
1222 1076 213
PvuII CAG'CTG
Cuts at: 0 1350 1386 2511
Size: 1350 36 1125
Fragments arranged by size:
1350 1125 36
SacII CC_GC'GG
Cuts at: 0 2120 2511
Size: 2120 391
SanDI GG'GwC_CC
Cuts at: 0 1922 2511
Size: 1922 589
SapI GCTCTTCn'nnn_
Cuts at: 0 618 2454 2511
Size: 618 1836 57
Fragments arranged by size:
1836 618 57
ScaI AGT'ACT
Cuts at: 0 1672 2511
Size: 1672 839
SfcI C'TryA_G
Cuts at: 0 1218 2280 2294 2511
Size: 1218 1062 14 217
Fragments arranged by size:
1218 1062 217 14
SfoI GGC'GCC
Cuts at: 0 344 2491 2511
Size: 344 2147 20
Fragments arranged by size:
2147 344 20
SmaI CCC'GGG
Cuts at: 0 24 169 1876 2511
Size: 24 145 1707 635
Fragments arranged by size:
1707 635 145 24
SrfI GCCC'GGGC
Cuts at: 0 1876 2511
Size: 1876 635
SspI AAT'ATT
Cuts at: 0 1548 2511
Size: 1548 963
StuI AGG'CCT
Cuts at: 0 259 754 1498 1647 2511
Size: 259 495 744 149 864
Fragments arranged by size:
864 744 495 259 149
StyI C'CwwG_G
Cuts at: 0 749 1573 1582 1808 2319 2511
Size: 749 824 9 226 511 192
Fragments arranged by size:
824 749 511 226 192 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 779 1495 1938 2143 2511
Size: 346 433 716 443 205 368
Fragments arranged by size:
716 443 433 368 346 205
TatI w'GTAC_w
Cuts at: 0 1670 2511
Size: 1670 841
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2336 2368 2511
Size: 2336 32 143
Fragments arranged by size:
2336 143 32
Tth111I GACn'n_nGTC
Cuts at: 0 2310 2511
Size: 2310 201
XmaI C'CCGG_G
Cuts at: 0 22 167 1874 2511
Size: 22 145 1707 637
Fragments arranged by size:
1707 637 145 22
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
EaeI EagI EarI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MscI MslI MspA1I NarI NcoI NsiI
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacII SanDI SapI ScaI SfcI SfoI SmaI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI
BglII BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsiWI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SbfI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SwaI XbaI XcmI
XhoI XmnI ZraI