Restriction map for
pF1KA0682
(Linear) (Six-base) MAPSORT of: KIAA0682.seq Check: 2187 from: 1 to: 2880
KIAA0682.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 883 2880
Size: 883 1997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 400 1713 1899 2012 2111 2257 2538
Size: 118 282 1313 186 113 99 146 281
Cuts at: 2538 2857 2880
Size: 319 23
Fragments arranged by size:
1313 319 282 281 186 146 118 113
99 23
AflIII A'CryG_T
Cuts at: 0 1421 2880
Size: 1421 1459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 80 2880
Size: 80 2800
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 379 411 502 534 2880
Size: 379 32 91 32 2346
Fragments arranged by size:
2346 379 91 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 263 2071 2754 2880
Size: 263 1808 683 126
Fragments arranged by size:
1808 683 263 126
ApaLI G'TGCA_C
Cuts at: 0 700 1124 2880
Size: 700 424 1756
Fragments arranged by size:
1756 700 424
ApoI r'AATT_y
Cuts at: 0 934 2880
Size: 934 1946
AvaI C'yCGr_G
Cuts at: 0 1165 2880
Size: 1165 1715
BanI G'GyrC_C
Cuts at: 0 942 1789 2880
Size: 942 847 1091
Fragments arranged by size:
1091 942 847
BanII G_rGCy'C
Cuts at: 0 473 903 1292 1895 1971 2552 2789 2880
Size: 473 430 389 603 76 581 237 91
Fragments arranged by size:
603 581 473 430 389 237 91 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 1974 2581 2770 2880
Size: 1974 607 189 110
Fragments arranged by size:
1974 607 189 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 219 253 2880
Size: 219 34 2627
Fragments arranged by size:
2627 219 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 381 994 1154 2880
Size: 381 613 160 1726
Fragments arranged by size:
1726 613 381 160
BglI GCCn_nnn'nGGC
Cuts at: 0 169 445 1829 1909 2880
Size: 169 276 1384 80 971
Fragments arranged by size:
1384 971 276 169 80
BlpI GC'TnA_GC
Cuts at: 0 467 2880
Size: 467 2413
Bme1580I G_kGCm'C
Cuts at: 0 704 1128 1470 2880
Size: 704 424 342 1410
Fragments arranged by size:
1410 704 424 342
BmgBI CAC'GTC
Cuts at: 0 2391 2880
Size: 2391 489
BmrI ACTGGGnnnn_n'
Cuts at: 0 745 2612 2880
Size: 745 1867 268
Fragments arranged by size:
1867 745 268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 756 998 2880
Size: 756 242 1882
Fragments arranged by size:
1882 756 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 308 391 1273 1909 1918 1955 1971
Size: 285 23 83 882 636 9 37 16
Cuts at: 1971 1981 2365 2740 2848 2880
Size: 10 384 375 108 32
Fragments arranged by size:
882 636 384 375 285 108 83 37
32 23 16 10 9
Bpu10I CC'TnA_GC
Cuts at: 0 1358 2880
Size: 1358 1522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2356 2880
Size: 2356 524
BsaI GGTCTCn'nnnn_
Cuts at: 0 810 1621 1807 2880
Size: 810 811 186 1073
Fragments arranged by size:
1073 811 810 186
BsaAI yAC'GTr
Cuts at: 0 1422 2880
Size: 1422 1458
BsaBI GATnn'nnATC
Cuts at: 0 2096 2880
Size: 2096 784
BsaHI Gr'CG_yC
Cuts at: 0 1979 2880
Size: 1979 901
BsaWI w'CCGG_w
Cuts at: 0 822 1201 2880
Size: 822 379 1679
Fragments arranged by size:
1679 822 379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 502 532 2880
Size: 394 30 78 30 2348
Fragments arranged by size:
2348 394 78 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 556 559 1195 1198 1258 2158 2161
Size: 58 498 3 636 3 60 900 3
Cuts at: 2161 2164 2875 2880
Size: 3 711 5
Fragments arranged by size:
900 711 636 498 60 58 5 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 652 805 1670 2049 2880
Size: 652 153 865 379 831
Fragments arranged by size:
865 831 652 379 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 800 2880
Size: 622 178 2080
Fragments arranged by size:
2080 622 178
BsiEI CG_ry'CG
Cuts at: 0 1802 1824 2771 2880
Size: 1802 22 947 109
Fragments arranged by size:
1802 947 109 22
BsiHKAI G_wGCw'C
Cuts at: 0 704 1128 1292 1364 2312 2552 2880
Size: 704 424 164 72 948 240 328
Fragments arranged by size:
948 704 424 328 240 164 72
BsmI GAATG_Cn'
Cuts at: 0 1336 1569 2880
Size: 1336 233 1311
Fragments arranged by size:
1336 1311 233
Bsp1286I G_dGCh'C
Cuts at: 0 473 704 903 1128 1292 1364 1470 1895
Size: 473 231 199 225 164 72 106 425
Cuts at: 1895 1971 2312 2552 2789 2880
Size: 76 341 240 237 91
Fragments arranged by size:
473 425 341 240 237 231 225 199
164 106 91 76 72
BspEI T'CCGG_A
Cuts at: 0 1201 2880
Size: 1201 1679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 352 883 1232 1386 2880
Size: 352 531 349 154 1494
Fragments arranged by size:
1494 531 352 349 154
BsrBI CCG'CTC
Cuts at: 0 2773 2880
Size: 2773 107
BsrDI GCAATG_nn'
Cuts at: 0 1029 2880
Size: 1029 1851
BsrFI r'CCGG_y
Cuts at: 0 492 2880
Size: 492 2388
BssSI C'ACGA_G
Cuts at: 0 1624 2782 2880
Size: 1624 1158 98
Fragments arranged by size:
1624 1158 98
BstAPI GCAn_nnn'nTGC
Cuts at: 0 798 2754 2880
Size: 798 1956 126
Fragments arranged by size:
1956 798 126
BstBI TT'CG_AA
Cuts at: 0 966 2880
Size: 966 1914
BstEII G'GTnAC_C
Cuts at: 0 2739 2880
Size: 2739 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1470 2502 2880
Size: 1470 1032 378
Fragments arranged by size:
1470 1032 378
BstYI r'GATC_y
Cuts at: 0 1248 2420 2498 2540 2822 2880
Size: 1248 1172 78 42 282 58
Fragments arranged by size:
1248 1172 282 78 58 42
BtgI C'CryG_G
Cuts at: 0 1568 2036 2318 2880
Size: 1568 468 282 562
Fragments arranged by size:
1568 562 468 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1566 2880
Size: 1566 1314
BtsI GCAGTG_nn'
Cuts at: 0 1868 2850 2880
Size: 1868 982 30
Fragments arranged by size:
1868 982 30
DraIII CAC_nnn'GTG
Cuts at: 0 885 1368 2394 2880
Size: 885 483 1026 486
Fragments arranged by size:
1026 885 486 483
DrdI GACnn_nn'nnGTC
Cuts at: 0 1711 2880
Size: 1711 1169
EaeI y'GGCC_r
Cuts at: 0 248 494 959 1193 1571 1643 1799 1821
Size: 248 246 465 234 378 72 156 22
Cuts at: 1821 2400 2768 2880
Size: 579 368 112
Fragments arranged by size:
579 465 378 368 248 246 234 156
112 72 22
EagI C'GGCC_G
Cuts at: 0 1799 1821 2768 2880
Size: 1799 22 947 112
Fragments arranged by size:
1799 947 112 22
EarI CTCTTCn'nnn_
Cuts at: 0 152 478 566 669 671 725 1022 1172
Size: 152 326 88 103 2 54 297 150
Cuts at: 1172 1267 2130 2135 2150 2165 2222 2263 2557
Size: 95 863 5 15 15 57 41 294
Cuts at: 2557 2656 2880
Size: 99 224
Fragments arranged by size:
863 326 297 294 224 152 150 103
99 95 88 57 54 41 15 15
5 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 732 1877 2572 2721 2775 2880
Size: 732 1145 695 149 54 105
Fragments arranged by size:
1145 732 695 149 105 54
EcoICRI GAG'CTC
Cuts at: 0 1290 2550 2880
Size: 1290 1260 330
Fragments arranged by size:
1290 1260 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 285 308 391 400 1273 1713 1899
Size: 118 167 23 83 9 873 440 186
Cuts at: 1899 1909 1918 1955 1971 1981 2012 2111 2257
Size: 10 9 37 16 10 31 99 146
Cuts at: 2257 2365 2538 2740 2848 2857 2880
Size: 108 173 202 108 9 23
Fragments arranged by size:
873 440 202 186 173 167 146 118
108 108 99 83 37 31 23 23
16 10 10 9 9 9
EcoO109I rG'GnC_Cy
Cuts at: 0 243 561 1188 2046 2751 2880
Size: 243 318 627 858 705 129
Fragments arranged by size:
858 705 627 318 243 129
EcoRI G'AATT_C
Cuts at: 0 934 2880
Size: 934 1946
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2685 2880
Size: 2685 195
HaeII r_GCGC'y
Cuts at: 0 1080 2880
Size: 1080 1800
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 2442 2474 2757 2789 2880
Size: 394 32 2016 32 283 32 91
Fragments arranged by size:
2016 394 283 91 32 32 32
HincII GTy'rAC
Cuts at: 0 367 2880
Size: 367 2513
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 2125 2795 2880
Size: 596 1529 670 85
Fragments arranged by size:
1529 670 596 85
MscI TGG'CCA
Cuts at: 0 961 2880
Size: 961 1919
MslI CAynn'nnrTG
Cuts at: 0 707 1009 1087 1348 2880
Size: 707 302 78 261 1532
Fragments arranged by size:
1532 707 302 261 78
MspA1I CmG'CkG
Cuts at: 0 60 598 805 2835 2877 2880
Size: 60 538 207 2030 42 3
Fragments arranged by size:
2030 538 207 60 42 3
NaeI GCC'GGC
Cuts at: 0 494 2880
Size: 494 2386
NcoI C'CATG_G
Cuts at: 0 2036 2318 2880
Size: 2036 282 562
Fragments arranged by size:
2036 562 282
NgoMIV G'CCGG_C
Cuts at: 0 492 2880
Size: 492 2388
NruI TCG'CGA
Cuts at: 0 6 2880
Size: 6 2874
NspI r_CATG'y
Cuts at: 0 277 2880
Size: 277 2603
PasI CC'CwG_GG
Cuts at: 0 1470 2880
Size: 1470 1410
PflMI CCAn_nnn'nTGG
Cuts at: 0 263 1148 2409 2880
Size: 263 885 1261 471
Fragments arranged by size:
1261 885 471 263
PfoI T'CCnGG_A
Cuts at: 0 208 398 1679 1886 2825 2880
Size: 208 190 1281 207 939 55
Fragments arranged by size:
1281 939 208 207 190 55
PmlI CAC'GTG
Cuts at: 0 1422 2880
Size: 1422 1458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 2880
Size: 502 32 2346
Fragments arranged by size:
2346 502 32
PpuMI rG'GwC_Cy
Cuts at: 0 243 561 1188 2046 2880
Size: 243 318 627 858 834
Fragments arranged by size:
858 834 627 318 243
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1213 1245 2880
Size: 1213 32 1635
Fragments arranged by size:
1635 1213 32
PstI C_TGCA'G
Cuts at: 0 94 761 1743 1811 2750 2880
Size: 94 667 982 68 939 130
Fragments arranged by size:
982 939 667 130 94 68
PvuII CAG'CTG
Cuts at: 0 60 598 805 2835 2877 2880
Size: 60 538 207 2030 42 3
Fragments arranged by size:
2030 538 207 60 42 3
RsrII CG'GwC_CG
Cuts at: 0 2580 2880
Size: 2580 300
SacI G_AGCT'C
Cuts at: 0 1292 2552 2880
Size: 1292 1260 328
Fragments arranged by size:
1292 1260 328
SanDI GG'GwC_CC
Cuts at: 0 243 2880
Size: 243 2637
SapI GCTCTTCn'nnn_
Cuts at: 0 478 1267 2263 2557 2880
Size: 478 789 996 294 323
Fragments arranged by size:
996 789 478 323 294
SbfI CC_TGCA'GG
Cuts at: 0 761 2750 2880
Size: 761 1989 130
Fragments arranged by size:
1989 761 130
SfcI C'TryA_G
Cuts at: 0 90 757 1739 1807 2746 2880
Size: 90 667 982 68 939 134
Fragments arranged by size:
982 939 667 134 90 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1909 2880
Size: 1909 971
SmlI C'TyrA_G
Cuts at: 0 2371 2880
Size: 2371 509
StuI AGG'CCT
Cuts at: 0 1375 1912 2880
Size: 1375 537 968
Fragments arranged by size:
1375 968 537
StyI C'CwwG_G
Cuts at: 0 1118 1325 1631 2036 2318 2880
Size: 1118 207 306 405 282 562
Fragments arranged by size:
1118 562 405 306 282 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1060 2707 2880
Size: 1060 1647 173
Fragments arranged by size:
1647 1060 173
TatI w'GTAC_w
Cuts at: 0 1064 1592 2188 2880
Size: 1064 528 596 692
Fragments arranged by size:
1064 692 596 528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1638 1875 2880
Size: 1638 237 1005
Fragments arranged by size:
1638 1005 237
XmnI GAAnn'nnTTC
Cuts at: 0 181 1261 2880
Size: 181 1080 1619
Fragments arranged by size:
1619 1080 181
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NruI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PsrI
PstI PvuII RsrII SacI SanDI SapI SbfI SfcI
SfiI SmlI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BclI BfrBI BglII BmtI BsiWI BsmBI
BspHI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraI
EcoNI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NsiI
PacI PciI PmeI PshAI PsiI PspOMI PspXI PvuI
SacII SalI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI