Restriction map for
pF1KA0678
(Linear) (Six-base) MAPSORT of: KIAA0678.seq Check: 4964 from: 1 to: 6729
KIAA0678.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 0 510 1902 3498 5033 6695 6729
Size: 510 1392 1596 1535 1662 34
Fragments arranged by size:
1662 1596 1535 1392 510 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1661 3043 3539 3957 4202 4331 4802 5756
Size: 1661 1382 496 418 245 129 471 954
Cuts at: 5756 5912 6087 6533 6729
Size: 156 175 446 196
Fragments arranged by size:
1661 1382 954 496 471 446 418 245
196 175 156 129
AflII C'TTAA_G
Cuts at: 0 1470 3076 6729
Size: 1470 1606 3653
Fragments arranged by size:
3653 1606 1470
AflIII A'CryG_T
Cuts at: 0 778 3146 4022 4403 6729
Size: 778 2368 876 381 2326
Fragments arranged by size:
2368 2326 876 778 381
AhdI GACnn_n'nnGTC
Cuts at: 0 3106 6729
Size: 3106 3623
AleI CACnn'nnGTG
Cuts at: 0 362 6729
Size: 362 6367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 560 1823 6729
Size: 560 1263 4906
Fragments arranged by size:
4906 1263 560
AlwNI CAG_nnn'CTG
Cuts at: 0 1117 3026 3270 5296 6313 6628 6729
Size: 1117 1909 244 2026 1017 315 101
Fragments arranged by size:
2026 1909 1117 1017 315 244 101
ApaLI G'TGCA_C
Cuts at: 0 2709 6729
Size: 2709 4020
ApoI r'AATT_y
Cuts at: 0 250 640 889 1540 2077 2137 2328 2554
Size: 250 390 249 651 537 60 191 226
Cuts at: 2554 3583 4051 4625 4960 5026 5692 6001 6729
Size: 1029 468 574 335 66 666 309 728
Fragments arranged by size:
1029 728 666 651 574 537 468 390
335 309 250 249 226 191 66 60
AseI AT'TA_AT
Cuts at: 0 419 6338 6729
Size: 419 5919 391
Fragments arranged by size:
5919 419 391
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3973 4006 4416 4449 6729
Size: 3973 33 410 33 2280
Fragments arranged by size:
3973 2280 410 33 33
BanI G'GyrC_C
Cuts at: 0 1570 1822 3623 6729
Size: 1570 252 1801 3106
Fragments arranged by size:
3106 1801 1570 252
BanII G_rGCy'C
Cuts at: 0 3033 4319 5619 6038 6729
Size: 3033 1286 1300 419 691
Fragments arranged by size:
3033 1300 1286 691 419
BbeI G_GCGC'C
Cuts at: 0 3627 6729
Size: 3627 3102
BbsI GAAGACnn'nnnn_
Cuts at: 0 2250 3667 5940 6729
Size: 2250 1417 2273 789
Fragments arranged by size:
2273 2250 1417 789
BbvCI CC'TCA_GC
Cuts at: 0 6116 6729
Size: 6116 613
BciVI GTATCCnnnnn_n'
Cuts at: 0 4174 4417 6607 6729
Size: 4174 243 2190 122
Fragments arranged by size:
4174 2190 243 122
BclI T'GATC_A
Cuts at: 0 1208 3152 5040 6729
Size: 1208 1944 1888 1689
Fragments arranged by size:
1944 1888 1689 1208
BfrBI ATG'CAT
Cuts at: 0 1455 2034 5309 6729
Size: 1455 579 3275 1420
Fragments arranged by size:
3275 1455 1420 579
BglI GCCn_nnn'nGGC
Cuts at: 0 6466 6729
Size: 6466 263
BglII A'GATC_T
Cuts at: 0 480 2001 6729
Size: 480 1521 4728
Fragments arranged by size:
4728 1521 480
BlpI GC'TnA_GC
Cuts at: 0 4824 6729
Size: 4824 1905
Bme1580I G_kGCm'C
Cuts at: 0 1440 1575 2713 3509 5318 6729
Size: 1440 135 1138 796 1809 1411
Fragments arranged by size:
1809 1440 1411 1138 796 135
BmtI G_CTAG'C
Cuts at: 0 4276 6729
Size: 4276 2453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 383 1555 2196 2879 3578 3627 4781
Size: 381 2 1172 641 683 699 49 1154
Cuts at: 4781 5131 6576 6650 6729
Size: 350 1445 74 79
Fragments arranged by size:
1445 1172 1154 699 683 641 381 350
79 74 49 2
Bpu10I CC'TnA_GC
Cuts at: 0 6116 6729
Size: 6116 613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1294 3017 3911 5973 6472 6729
Size: 354 940 1723 894 2062 499 257
Fragments arranged by size:
2062 1723 940 894 499 354 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 1186 6262 6729
Size: 1186 5076 467
Fragments arranged by size:
5076 1186 467
BsaBI GATnn'nnATC
Cuts at: 0 2024 4004 6729
Size: 2024 1980 2725
Fragments arranged by size:
2725 2024 1980
BsaHI Gr'CG_yC
Cuts at: 0 3624 6729
Size: 3624 3105
BsaWI w'CCGG_w
Cuts at: 0 3794 6729
Size: 3794 2935
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 6509 6539 6729
Size: 6509 30 190
Fragments arranged by size:
6509 190 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1311 5957 6729
Size: 1311 4646 772
Fragments arranged by size:
4646 1311 772
BseYI C'CCAG_C
Cuts at: 0 1741 3026 3071 6453 6729
Size: 1741 1285 45 3382 276
Fragments arranged by size:
3382 1741 1285 276 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5386 6729
Size: 5386 1343
BsiHKAI G_wGCw'C
Cuts at: 0 266 1623 2713 3696 4319 5846 6038 6729
Size: 266 1357 1090 983 623 1527 192 691
Fragments arranged by size:
1527 1357 1090 983 691 623 266 192
BsmI GAATG_Cn'
Cuts at: 0 4471 4685 5311 6729
Size: 4471 214 626 1418
Fragments arranged by size:
4471 1418 626 214
Bsp1286I G_dGCh'C
Cuts at: 0 266 1440 1575 1623 2713 3033 3509 3696
Size: 266 1174 135 48 1090 320 476 187
Cuts at: 3696 4319 5318 5619 5846 6038 6729
Size: 623 999 301 227 192 691
Fragments arranged by size:
1174 1090 999 691 623 476 320 301
266 227 192 187 135 48
BspHI T'CATG_A
Cuts at: 0 2973 3383 5766 6729
Size: 2973 410 2383 963
Fragments arranged by size:
2973 2383 963 410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 6643 6689 6729
Size: 6643 46 40
Fragments arranged by size:
6643 46 40
BsrDI GCAATG_nn'
Cuts at: 0 613 2066 2192 2883 3530 5473 5555 6176
Size: 613 1453 126 691 647 1943 82 621
Cuts at: 6176 6500 6729
Size: 324 229
Fragments arranged by size:
1943 1453 691 647 621 613 324 229
126 82
BsrGI T'GTAC_A
Cuts at: 0 3389 5745 6729
Size: 3389 2356 984
Fragments arranged by size:
3389 2356 984
BssSI C'ACGA_G
Cuts at: 0 2339 6729
Size: 2339 4390
BstAPI GCAn_nnn'nTGC
Cuts at: 0 5131 5560 6729
Size: 5131 429 1169
Fragments arranged by size:
5131 1169 429
BstBI TT'CG_AA
Cuts at: 0 2235 5676 6729
Size: 2235 3441 1053
Fragments arranged by size:
3441 2235 1053
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5457 6729
Size: 5457 1272
BstYI r'GATC_y
Cuts at: 0 24 480 2001 2157 2274 2784 4092 4506
Size: 24 456 1521 156 117 510 1308 414
Cuts at: 4506 5957 6729
Size: 1451 772
Fragments arranged by size:
1521 1451 1308 772 510 456 414 156
117 24
BstZ17I GTA'TAC
Cuts at: 0 511 3499 6729
Size: 511 2988 3230
Fragments arranged by size:
3230 2988 511
Bsu36I CC'TnA_GG
Cuts at: 0 1075 5870 6729
Size: 1075 4795 859
Fragments arranged by size:
4795 1075 859
BtgI C'CryG_G
Cuts at: 0 1721 6254 6729
Size: 1721 4533 475
Fragments arranged by size:
4533 1721 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1038 1864 2560 6729
Size: 1038 826 696 4169
Fragments arranged by size:
4169 1038 826 696
BtsI GCAGTG_nn'
Cuts at: 0 231 2866 3618 6729
Size: 231 2635 752 3111
Fragments arranged by size:
3111 2635 752 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2971 3006 4061 4096 6729
Size: 2971 35 1055 35 2633
Fragments arranged by size:
2971 2633 1055 35 35
DraI TTT'AAA
Cuts at: 0 248 1701 5850 6729
Size: 248 1453 4149 879
Fragments arranged by size:
4149 1453 879 248
DraIII CAC_nnn'GTG
Cuts at: 0 3161 6729
Size: 3161 3568
EaeI y'GGCC_r
Cuts at: 0 3808 5186 6729
Size: 3808 1378 1543
Fragments arranged by size:
3808 1543 1378
EarI CTCTTCn'nnn_
Cuts at: 0 557 1189 1505 2284 2749 3458 4141 4307
Size: 557 632 316 779 465 709 683 166
Cuts at: 4307 4317 4670 5342 5468 6106 6729
Size: 10 353 672 126 638 623
Fragments arranged by size:
779 709 683 672 638 632 623 557
465 353 316 166 126 10
EcoICRI GAG'CTC
Cuts at: 0 4317 6036 6729
Size: 4317 1719 693
Fragments arranged by size:
4317 1719 693
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 383 1555 1661 2196 2879 3043 3539
Size: 381 2 1172 106 535 683 164 496
Cuts at: 3539 3578 3627 3957 4202 4331 4781 4802 5131
Size: 39 49 330 245 129 450 21 329
Cuts at: 5131 5756 5912 6087 6533 6576 6650 6729
Size: 625 156 175 446 43 74 79
Fragments arranged by size:
1172 683 625 535 496 450 446 381
330 329 245 175 164 156 129 106
79 74 49 43 39 21 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2464 4615 6729
Size: 2464 2151 2114
Fragments arranged by size:
2464 2151 2114
EcoO109I rG'GnC_Cy
Cuts at: 0 2665 6132 6625 6729
Size: 2665 3467 493 104
Fragments arranged by size:
3467 2665 493 104
EcoRI G'AATT_C
Cuts at: 0 640 6729
Size: 640 6089
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 96 1516 2176 2377 3862 6729
Size: 96 1420 660 201 1485 2867
Fragments arranged by size:
2867 1485 1420 660 201 96
HaeII r_GCGC'y
Cuts at: 0 3627 6729
Size: 3627 3102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3092 3124 4463 4495 5319 5351 5715 5747
Size: 3092 32 1339 32 824 32 364 32
Cuts at: 5747 5797 5829 6729
Size: 50 32 900
Fragments arranged by size:
3092 1339 900 824 364 50 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 4290 5421 6729
Size: 4290 1131 1308
Fragments arranged by size:
4290 1308 1131
HindIII A'AGCT_T
Cuts at: 0 1493 3829 3965 4821 6729
Size: 1493 2336 136 856 1908
Fragments arranged by size:
2336 1908 1493 856 136
KasI G'GCGC_C
Cuts at: 0 3623 6729
Size: 3623 3106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 159 4831 5130 5919 6729
Size: 69 90 4672 299 789 810
Fragments arranged by size:
4672 810 789 299 90 69
MscI TGG'CCA
Cuts at: 0 3810 5188 6729
Size: 3810 1378 1541
Fragments arranged by size:
3810 1541 1378
MslI CAynn'nnrTG
Cuts at: 0 362 2695 2843 4486 5182 6729
Size: 362 2333 148 1643 696 1547
Fragments arranged by size:
2333 1643 1547 696 362 148
MspA1I CmG'CkG
Cuts at: 0 1927 6120 6729
Size: 1927 4193 609
Fragments arranged by size:
4193 1927 609
NarI GG'CG_CC
Cuts at: 0 3624 6729
Size: 3624 3105
NcoI C'CATG_G
Cuts at: 0 1721 6254 6729
Size: 1721 4533 475
Fragments arranged by size:
4533 1721 475
NdeI CA'TA_TG
Cuts at: 0 4047 6729
Size: 4047 2682
NheI G'CTAG_C
Cuts at: 0 4272 6729
Size: 4272 2457
NruI TCG'CGA
Cuts at: 0 1355 6729
Size: 1355 5374
NsiI A_TGCA'T
Cuts at: 0 1457 2036 5311 6729
Size: 1457 579 3275 1418
Fragments arranged by size:
3275 1457 1418 579
NspI r_CATG'y
Cuts at: 0 35 782 1044 1165 2038 3150 4026 4407
Size: 35 747 262 121 873 1112 876 381
Cuts at: 4407 4836 6729
Size: 429 1893
Fragments arranged by size:
1893 1112 876 873 747 429 381 262
121 35
PciI A'CATG_T
Cuts at: 0 778 3146 4022 4403 6729
Size: 778 2368 876 381 2326
Fragments arranged by size:
2368 2326 876 778 381
PflMI CCAn_nnn'nTGG
Cuts at: 0 3026 6729
Size: 3026 3703
PfoI T'CCnGG_A
Cuts at: 0 399 2422 6729
Size: 399 2023 4307
Fragments arranged by size:
4307 2023 399
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1267 1299 6729
Size: 1267 32 5430
Fragments arranged by size:
5430 1267 32
PpuMI rG'GwC_Cy
Cuts at: 0 2665 6132 6625 6729
Size: 2665 3467 493 104
Fragments arranged by size:
3467 2665 493 104
PshAI GACnn'nnGTC
Cuts at: 0 3231 6729
Size: 3231 3498
PsiI TTA'TAA
Cuts at: 0 895 4169 6729
Size: 895 3274 2560
Fragments arranged by size:
3274 2560 895
PvuII CAG'CTG
Cuts at: 0 1927 6120 6729
Size: 1927 4193 609
Fragments arranged by size:
4193 1927 609
SacI G_AGCT'C
Cuts at: 0 4319 6038 6729
Size: 4319 1719 691
Fragments arranged by size:
4319 1719 691
SapI GCTCTTCn'nnn_
Cuts at: 0 4307 5468 6729
Size: 4307 1161 1261
Fragments arranged by size:
4307 1261 1161
ScaI AGT'ACT
Cuts at: 0 440 6729
Size: 440 6289
SfcI C'TryA_G
Cuts at: 0 257 456 2858 4265 4440 4600 5034 6692
Size: 257 199 2402 1407 175 160 434 1658
Cuts at: 6692 6729
Size: 37
Fragments arranged by size:
2402 1658 1407 434 257 199 175 160
37
SfoI GGC'GCC
Cuts at: 0 3625 6729
Size: 3625 3104
SmlI C'TyrA_G
Cuts at: 0 369 1273 1470 3032 3076 3926 5952 6487
Size: 369 904 197 1562 44 850 2026 535
Cuts at: 6487 6729
Size: 242
Fragments arranged by size:
2026 1562 904 850 535 369 242 197
44
SphI G_CATG'C
Cuts at: 0 1044 4836 6729
Size: 1044 3792 1893
Fragments arranged by size:
3792 1893 1044
SspI AAT'ATT
Cuts at: 0 465 652 813 916 1140 2388 3762 5427
Size: 465 187 161 103 224 1248 1374 1665
Cuts at: 5427 6729
Size: 1302
Fragments arranged by size:
1665 1374 1302 1248 465 224 187 161
103
StyI C'CwwG_G
Cuts at: 0 1721 4641 4764 6254 6714 6729
Size: 1721 2920 123 1490 460 15
Fragments arranged by size:
2920 1721 1490 460 123 15
TatI w'GTAC_w
Cuts at: 0 438 3389 4410 5527 5745 6729
Size: 438 2951 1021 1117 218 984
Fragments arranged by size:
2951 1117 1021 984 438 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1326 1358 3234 3266 4115 4147 4325 4357
Size: 1326 32 1876 32 849 32 178 32
Cuts at: 4357 4899 4931 6509 6541 6729
Size: 542 32 1578 32 188
Fragments arranged by size:
1876 1578 1326 849 542 188 178 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 5416 6729
Size: 5416 1313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1558 2151 3602 4925 6729
Size: 1558 593 1451 1323 1804
Fragments arranged by size:
1804 1558 1451 1323 593
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AleI AlfI AlwNI
ApaLI ApoI AseI BaeI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NruI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PshAI PsiI PvuII
SacI SapI ScaI SfcI SfoI SmlI SphI SspI
StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AloI ApaI
AscI AsiSI AvaI AvrII BamHI BcgI BmgBI BmrI
BplI BsaAI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BstEII ClaI DrdI EagI EciI EcoRV FseI
FspI FspAI HpaI KpnI MfeI MluI NaeI NgoMIV
NotI PacI PasI PmeI PmlI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI TaqII XbaI XhoI XmaI XmnI ZraI