Restriction map for
pF1KA0675
(Linear) (Six-base) MAPSORT of: KIAA0675.seq Check: 3026 from: 1 to: 3624
KIAA0675.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 1700 2097 2726 3624
Size: 1700 397 629 898
Fragments arranged by size:
1700 898 629 397
AflIII A'CryG_T
Cuts at: 0 3547 3624
Size: 3547 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2837 3624
Size: 2837 787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2137 2169 3624
Size: 2137 32 1455
Fragments arranged by size:
2137 1455 32
AlwNI CAG_nnn'CTG
Cuts at: 0 469 3040 3624
Size: 469 2571 584
Fragments arranged by size:
2571 584 469
ApoI r'AATT_y
Cuts at: 0 19 478 515 601 866 888 1004 1050
Size: 19 459 37 86 265 22 116 46
Cuts at: 1050 1126 3502 3586 3624
Size: 76 2376 84 38
Fragments arranged by size:
2376 459 265 116 86 84 76 46
38 37 22 19
AseI AT'TA_AT
Cuts at: 0 182 386 1898 3624
Size: 182 204 1512 1726
Fragments arranged by size:
1726 1512 204 182
AvaI C'yCGr_G
Cuts at: 0 2967 3624
Size: 2967 657
AvrII C'CTAG_G
Cuts at: 0 1704 3624
Size: 1704 1920
BanII G_rGCy'C
Cuts at: 0 2203 3003 3030 3624
Size: 2203 800 27 594
Fragments arranged by size:
2203 800 594 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 1996 2030 2153 2907 3624
Size: 1996 34 123 754 717
Fragments arranged by size:
1996 754 717 123 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 908 3624
Size: 908 2716
BclI T'GATC_A
Cuts at: 0 912 3624
Size: 912 2712
BglI GCCn_nnn'nGGC
Cuts at: 0 2746 3624
Size: 2746 878
BglII A'GATC_T
Cuts at: 0 696 1950 2491 3624
Size: 696 1254 541 1133
Fragments arranged by size:
1254 1133 696 541
BlpI GC'TnA_GC
Cuts at: 0 2202 3624
Size: 2202 1422
BmgBI CAC'GTC
Cuts at: 0 553 3624
Size: 553 3071
BmrI ACTGGGnnnn_n'
Cuts at: 0 1916 2861 3056 3237 3624
Size: 1916 945 195 181 387
Fragments arranged by size:
1916 945 387 195 181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 448 1689 2217 3624
Size: 448 1241 528 1407
Fragments arranged by size:
1407 1241 528 448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1939 2825 3381 3452 3624
Size: 1939 886 556 71 172
Fragments arranged by size:
1939 886 556 172 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2847 3624
Size: 2847 777
BsaI GGTCTCn'nnnn_
Cuts at: 0 1564 3624
Size: 1564 2060
BsaAI yAC'GTr
Cuts at: 0 2544 3624
Size: 2544 1080
BsaBI GATnn'nnATC
Cuts at: 0 499 1685 3624
Size: 499 1186 1939
Fragments arranged by size:
1939 1186 499
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2823 2853 3038 3068 3624
Size: 2823 30 185 30 556
Fragments arranged by size:
2823 556 185 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1244 2846 3624
Size: 1244 1602 778
Fragments arranged by size:
1602 1244 778
BseYI C'CCAG_C
Cuts at: 0 3341 3601 3624
Size: 3341 260 23
Fragments arranged by size:
3341 260 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 1427 3086 3579 3624
Size: 855 572 1659 493 45
Fragments arranged by size:
1659 855 572 493 45
BsiHKAI G_wGCw'C
Cuts at: 0 573 3624
Size: 573 3051
BsmI GAATG_Cn'
Cuts at: 0 2742 2770 3624
Size: 2742 28 854
Fragments arranged by size:
2742 854 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2982 3041 3624
Size: 2982 59 583
Fragments arranged by size:
2982 583 59
Bsp1286I G_dGCh'C
Cuts at: 0 573 2203 3003 3030 3624
Size: 573 1630 800 27 594
Fragments arranged by size:
1630 800 594 573 27
BspHI T'CATG_A
Cuts at: 0 3092 3453 3624
Size: 3092 361 171
Fragments arranged by size:
3092 361 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3058 3624
Size: 3058 566
BsrDI GCAATG_nn'
Cuts at: 0 985 2147 3624
Size: 985 1162 1477
Fragments arranged by size:
1477 1162 985
BsrFI r'CCGG_y
Cuts at: 0 3605 3624
Size: 3605 19
BsrGI T'GTAC_A
Cuts at: 0 952 3624
Size: 952 2672
BstEII G'GTnAC_C
Cuts at: 0 3595 3624
Size: 3595 29
BstYI r'GATC_y
Cuts at: 0 657 696 1950 2491 3624
Size: 657 39 1254 541 1133
Fragments arranged by size:
1254 1133 657 541 39
Bsu36I CC'TnA_GG
Cuts at: 0 2776 3624
Size: 2776 848
BtgI C'CryG_G
Cuts at: 0 3526 3624
Size: 3526 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 579 3624
Size: 579 3045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 182 217 3624
Size: 182 35 3407
Fragments arranged by size:
3407 182 35
DraI TTT'AAA
Cuts at: 0 693 893 1124 1430 3624
Size: 693 200 231 306 2194
Fragments arranged by size:
2194 693 306 231 200
EaeI y'GGCC_r
Cuts at: 0 2988 3624
Size: 2988 636
EarI CTCTTCn'nnn_
Cuts at: 0 1799 2048 3416 3428 3624
Size: 1799 249 1368 12 196
Fragments arranged by size:
1799 1368 249 196 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1939 2825 3381 3452 3624
Size: 1939 886 556 71 172
Fragments arranged by size:
1939 886 556 172 71
EcoO109I rG'GnC_Cy
Cuts at: 0 1910 3382 3624
Size: 1910 1472 242
Fragments arranged by size:
1910 1472 242
EcoRI G'AATT_C
Cuts at: 0 3586 3624
Size: 3586 38
EcoRV GAT'ATC
Cuts at: 0 1079 1530 3624
Size: 1079 451 2094
Fragments arranged by size:
2094 1079 451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 240 361 718 1291 2494 3624
Size: 240 121 357 573 1203 1130
Fragments arranged by size:
1203 1130 573 357 240 121
HaeII r_GCGC'y
Cuts at: 0 3040 3624
Size: 3040 584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1100 1132 1311 1343 1542 1574 1897 1929
Size: 1100 32 179 32 199 32 323 32
Cuts at: 1929 3448 3480 3624
Size: 1519 32 144
Fragments arranged by size:
1519 1100 323 199 179 144 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 97 144 3624
Size: 97 47 3480
Fragments arranged by size:
3480 97 47
HindIII A'AGCT_T
Cuts at: 0 612 1159 3624
Size: 612 547 2465
Fragments arranged by size:
2465 612 547
HpaI GTT'AAC
Cuts at: 0 144 3624
Size: 144 3480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2713 3577 3624
Size: 2713 864 47
Fragments arranged by size:
2713 864 47
MscI TGG'CCA
Cuts at: 0 2990 3624
Size: 2990 634
MslI CAynn'nnrTG
Cuts at: 0 1286 1430 3624
Size: 1286 144 2194
Fragments arranged by size:
2194 1286 144
MspA1I CmG'CkG
Cuts at: 0 2206 3624
Size: 2206 1418
NaeI GCC'GGC
Cuts at: 0 3607 3624
Size: 3607 17
NcoI C'CATG_G
Cuts at: 0 3526 3624
Size: 3526 98
NdeI CA'TA_TG
Cuts at: 0 2037 3624
Size: 2037 1587
NgoMIV G'CCGG_C
Cuts at: 0 3605 3624
Size: 3605 19
NspI r_CATG'y
Cuts at: 0 3551 3624
Size: 3551 73
PciI A'CATG_T
Cuts at: 0 3547 3624
Size: 3547 77
PfoI T'CCnGG_A
Cuts at: 0 1955 3624
Size: 1955 1669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1558 1590 3624
Size: 1558 32 2034
Fragments arranged by size:
2034 1558 32
PpuMI rG'GwC_Cy
Cuts at: 0 1910 3382 3624
Size: 1910 1472 242
Fragments arranged by size:
1910 1472 242
PsiI TTA'TAA
Cuts at: 0 812 1169 1420 2104 2273 3624
Size: 812 357 251 684 169 1351
Fragments arranged by size:
1351 812 684 357 251 169
PstI C_TGCA'G
Cuts at: 0 1500 2961 3624
Size: 1500 1461 663
Fragments arranged by size:
1500 1461 663
PvuII CAG'CTG
Cuts at: 0 2206 3624
Size: 2206 1418
SapI GCTCTTCn'nnn_
Cuts at: 0 2048 3428 3624
Size: 2048 1380 196
Fragments arranged by size:
2048 1380 196
SfcI C'TryA_G
Cuts at: 0 662 1496 1768 2157 2219 2957 3624
Size: 662 834 272 389 62 738 667
Fragments arranged by size:
834 738 667 662 389 272 62
SmaI CCC'GGG
Cuts at: 0 2969 3624
Size: 2969 655
SmlI C'TyrA_G
Cuts at: 0 2826 3624
Size: 2826 798
SnaBI TAC'GTA
Cuts at: 0 2544 3624
Size: 2544 1080
SspI AAT'ATT
Cuts at: 0 390 1232 3624
Size: 390 842 2392
Fragments arranged by size:
2392 842 390
StyI C'CwwG_G
Cuts at: 0 1617 1704 3526 3624
Size: 1617 87 1822 98
Fragments arranged by size:
1822 1617 98 87
SwaI ATTT'AAAT
Cuts at: 0 1430 3624
Size: 1430 2194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1125 1324 1461 2880 3624
Size: 1125 199 137 1419 744
Fragments arranged by size:
1419 1125 744 199 137
TatI w'GTAC_w
Cuts at: 0 952 1850 2786 3624
Size: 952 898 936 838
Fragments arranged by size:
952 936 898 838
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3038 3070 3269 3301 3624
Size: 3038 32 199 32 323
Fragments arranged by size:
3038 323 199 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2029 3624
Size: 2029 1595
XbaI T'CTAG_A
Cuts at: 0 2631 3624
Size: 2631 993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 388 1329 3356 3624
Size: 388 941 2027 268
Fragments arranged by size:
2027 941 388 268
XmaI C'CCGG_G
Cuts at: 0 2967 3624
Size: 2967 657
XmnI GAAnn'nnTTC
Cuts at: 0 296 880 2705 3624
Size: 296 584 1825 919
Fragments arranged by size:
1825 919 584 296
Enzymes that do cut:
AccI AflIII AlfI AloI AlwNI ApoI AseI AvaI
AvrII BanII BbsI BciVI BclI BglI BglII BlpI
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BstEII BstYI Bsu36I
BtgI BtgZI CspCI DraI EaeI EarI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PciI PfoI PpiI PpuMI PsiI PstI PvuII
SapI SfcI SmaI SmlI SnaBI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI ApaI ApaLI AscI AsiSI BaeI BamHI
BanI BbeI BbvCI BcgI BfrBI Bme1580I BmtI Bpu10I
BsaHI BsaWI BsiEI BsiWI BspEI BsrBI BssHII BssSI
BstAPI BstBI BstXI BstZ17I BtsI ClaI DraIII DrdI
EagI EciI EcoICRI EcoNI FseI FspI FspAI KasI
KpnI MfeI MluI NarI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SpeI SphI
SrfI StuI XhoI ZraI