Restriction map for
pF1KA0671
(Linear) (Six-base) MAPSORT of: KIAA0671.seq Check: 9556 from: 1 to: 1608
KIAA0671.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1469 1608
Size: 1469 139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1608
Size: 1217 391
AflII C'TTAA_G
Cuts at: 0 174 1608
Size: 174 1434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1225 1608
Size: 1225 383
AlwNI CAG_nnn'CTG
Cuts at: 0 715 1608
Size: 715 893
ApaLI G'TGCA_C
Cuts at: 0 1482 1608
Size: 1482 126
ApoI r'AATT_y
Cuts at: 0 219 1608
Size: 219 1389
AseI AT'TA_AT
Cuts at: 0 637 1608
Size: 637 971
BanII G_rGCy'C
Cuts at: 0 724 1511 1608
Size: 724 787 97
Fragments arranged by size:
787 724 97
BbvCI CC'TCA_GC
Cuts at: 0 155 1608
Size: 155 1453
BciVI GTATCCnnnnn_n'
Cuts at: 0 540 972 1608
Size: 540 432 636
Fragments arranged by size:
636 540 432
Bme1580I G_kGCm'C
Cuts at: 0 1486 1608
Size: 1486 122
BmrI ACTGGGnnnn_n'
Cuts at: 0 208 904 1155 1379 1608
Size: 208 696 251 224 229
Fragments arranged by size:
696 251 229 224 208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1608
Size: 1052 556
Bpu10I CC'TnA_GC
Cuts at: 0 155 1213 1608
Size: 155 1058 395
Fragments arranged by size:
1058 395 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1608
Size: 664 944
BsaAI yAC'GTr
Cuts at: 0 1490 1608
Size: 1490 118
BsaHI Gr'CG_yC
Cuts at: 0 1319 1608
Size: 1319 289
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 1608
Size: 82 30 1496
Fragments arranged by size:
1496 82 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 143 1335 1608
Size: 143 1192 273
Fragments arranged by size:
1192 273 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1608
Size: 854 754
BsiHKAI G_wGCw'C
Cuts at: 0 1486 1608
Size: 1486 122
Bsp1286I G_dGCh'C
Cuts at: 0 724 1486 1511 1608
Size: 724 762 25 97
Fragments arranged by size:
762 724 97 25
BspHI T'CATG_A
Cuts at: 0 60 1608
Size: 60 1548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1469 1608
Size: 1469 139
BsrDI GCAATG_nn'
Cuts at: 0 1408 1608
Size: 1408 200
BsrGI T'GTAC_A
Cuts at: 0 480 1608
Size: 480 1128
BssSI C'ACGA_G
Cuts at: 0 1105 1608
Size: 1105 503
BstBI TT'CG_AA
Cuts at: 0 214 1608
Size: 214 1394
BstYI r'GATC_y
Cuts at: 0 1270 1380 1608
Size: 1270 110 228
Fragments arranged by size:
1270 228 110
BtgI C'CryG_G
Cuts at: 0 355 1608
Size: 355 1253
BtsI GCAGTG_nn'
Cuts at: 0 1098 1300 1608
Size: 1098 202 308
Fragments arranged by size:
1098 308 202
DraI TTT'AAA
Cuts at: 0 1541 1608
Size: 1541 67
DraIII CAC_nnn'GTG
Cuts at: 0 992 1608
Size: 992 616
EarI CTCTTCn'nnn_
Cuts at: 0 58 324 968 1246 1608
Size: 58 266 644 278 362
Fragments arranged by size:
644 362 278 266 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1246 1608
Size: 1246 362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1217 1608
Size: 1052 165 391
Fragments arranged by size:
1052 391 165
EcoRI G'AATT_C
Cuts at: 0 219 1608
Size: 219 1389
EcoRV GAT'ATC
Cuts at: 0 1028 1608
Size: 1028 580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 618 805 1608
Size: 618 187 803
Fragments arranged by size:
803 618 187
FspI TGC'GCA
Cuts at: 0 1226 1608
Size: 1226 382
HaeII r_GCGC'y
Cuts at: 0 465 1608
Size: 465 1143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 325 357 1580 1608
Size: 82 32 211 32 1223 28
Fragments arranged by size:
1223 211 82 32 32 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1562 1608
Size: 125 1437 46
Fragments arranged by size:
1437 125 46
MslI CAynn'nnrTG
Cuts at: 0 1046 1608
Size: 1046 562
NcoI C'CATG_G
Cuts at: 0 355 1608
Size: 355 1253
NspI r_CATG'y
Cuts at: 0 352 1282 1398 1608
Size: 352 930 116 210
Fragments arranged by size:
930 352 210 116
PflMI CCAn_nnn'nTGG
Cuts at: 0 361 1608
Size: 361 1247
PfoI T'CCnGG_A
Cuts at: 0 912 1608
Size: 912 696
PsiI TTA'TAA
Cuts at: 0 1376 1556 1608
Size: 1376 180 52
Fragments arranged by size:
1376 180 52
PstI C_TGCA'G
Cuts at: 0 1454 1480 1608
Size: 1454 26 128
Fragments arranged by size:
1454 128 26
SfcI C'TryA_G
Cuts at: 0 512 767 1450 1476 1608
Size: 512 255 683 26 132
Fragments arranged by size:
683 512 255 132 26
SmlI C'TyrA_G
Cuts at: 0 174 643 1608
Size: 174 469 965
Fragments arranged by size:
965 469 174
SnaBI TAC'GTA
Cuts at: 0 1490 1608
Size: 1490 118
SphI G_CATG'C
Cuts at: 0 1282 1608
Size: 1282 326
SspI AAT'ATT
Cuts at: 0 186 1608
Size: 186 1422
StyI C'CwwG_G
Cuts at: 0 355 1608
Size: 355 1253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 1608
Size: 46 1562
TatI w'GTAC_w
Cuts at: 0 480 1608
Size: 480 1128
XmnI GAAnn'nnTTC
Cuts at: 0 28 52 333 1178 1608
Size: 28 24 281 845 430
Fragments arranged by size:
845 430 281 28 24
Enzymes that do cut:
AarI AcuI AflII AlfI AlwNI ApaLI ApoI AseI
BanII BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstBI BstYI BtgI BtsI
DraI DraIII EarI EciI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I MmeI MslI NcoI NspI PflMI
PfoI PsiI PstI SfcI SmlI SnaBI SphI SspI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI ApaI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BsaI BsaBI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BssHII BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DrdI EaeI EagI EcoICRI EcoNI EcoO109I FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI