Restriction map for
pF1KA0651
(Linear) (Six-base) MAPSORT of: KIAA0651.seq Check: 2516 from: 1 to: 3240
KIAA0651.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
Acc65I G'GTAC_C
Cuts at: 0 280 3240
Size: 280 2960
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 601 1063 1820 3240
Size: 297 304 462 757 1420
Fragments arranged by size:
1420 757 462 304 297
AleI CACnn'nnGTG
Cuts at: 0 74 3240
Size: 74 3166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 973 1090 3240
Size: 973 117 2150
Fragments arranged by size:
2150 973 117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2563 2595 3240
Size: 2563 32 645
Fragments arranged by size:
2563 645 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1116 1194 1524 1654 3069 3240
Size: 1116 78 330 130 1415 171
Fragments arranged by size:
1415 1116 330 171 130 78
ApaI G_GGCC'C
Cuts at: 0 1602 1641 1666 2593 2910 3240
Size: 1602 39 25 927 317 330
Fragments arranged by size:
1602 927 330 317 39 25
ApaLI G'TGCA_C
Cuts at: 0 1933 3240
Size: 1933 1307
AvaI C'yCGr_G
Cuts at: 0 284 324 350 1352 1634 1946 2321 2627
Size: 284 40 26 1002 282 312 375 306
Cuts at: 2627 2816 3240
Size: 189 424
Fragments arranged by size:
1002 424 375 312 306 284 282 189
40 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 272 305 1411 1444 3240
Size: 272 33 1106 33 1796
Fragments arranged by size:
1796 1106 272 33 33
BamHI G'GATC_C
Cuts at: 0 791 1967 2922 3240
Size: 791 1176 955 318
Fragments arranged by size:
1176 955 791 318
BanI G'GyrC_C
Cuts at: 0 280 401 3240
Size: 280 121 2839
Fragments arranged by size:
2839 280 121
BanII G_rGCy'C
Cuts at: 0 694 1139 1602 1641 1666 2593 2910 3079
Size: 694 445 463 39 25 927 317 169
Cuts at: 3079 3240
Size: 161
Fragments arranged by size:
927 694 463 445 317 169 161 39
25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1732 3240
Size: 1732 1508
BbvCI CC'TCA_GC
Cuts at: 0 1162 2894 3240
Size: 1162 1732 346
Fragments arranged by size:
1732 1162 346
BciVI GTATCCnnnnn_n'
Cuts at: 0 198 231 3240
Size: 198 33 3009
Fragments arranged by size:
3009 198 33
BclI T'GATC_A
Cuts at: 0 1901 3240
Size: 1901 1339
BglI GCCn_nnn'nGGC
Cuts at: 0 1670 2665 2845 2905 3240
Size: 1670 995 180 60 335
Fragments arranged by size:
1670 995 335 180 60
BglII A'GATC_T
Cuts at: 0 1613 3240
Size: 1613 1627
Bme1580I G_kGCm'C
Cuts at: 0 404 1201 1602 1641 1666 1937 2091 2593
Size: 404 797 401 39 25 271 154 502
Cuts at: 2593 2823 2910 3240
Size: 230 87 330
Fragments arranged by size:
797 502 404 401 330 271 230 154
87 39 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 219 1546 1642 3079 3240
Size: 219 1327 96 1437 161
Fragments arranged by size:
1437 1327 219 161 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1774 2806 2848 3240
Size: 1609 165 1032 42 392
Fragments arranged by size:
1609 1032 392 165 42
Bpu10I CC'TnA_GC
Cuts at: 0 1162 2594 2894 3240
Size: 1162 1432 300 346
Fragments arranged by size:
1432 1162 346 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1390 2239 2985 3240
Size: 1390 849 746 255
Fragments arranged by size:
1390 849 746 255
BsaHI Gr'CG_yC
Cuts at: 0 1185 3240
Size: 1185 2055
BsaWI w'CCGG_w
Cuts at: 0 334 1379 1955 3240
Size: 334 1045 576 1285
Fragments arranged by size:
1285 1045 576 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1267 1297 1543 1573 2596 2626 3240
Size: 1267 30 246 30 1023 30 614
Fragments arranged by size:
1267 1023 614 246 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 285 699 1522 1525 2503 2743 3139 3211
Size: 285 414 823 3 978 240 396 72
Cuts at: 3211 3240
Size: 29
Fragments arranged by size:
978 823 414 396 285 240 72 29
3
BseYI C'CCAG_C
Cuts at: 0 2552 2650 2889 2986 3240
Size: 2552 98 239 97 254
Fragments arranged by size:
2552 254 239 98 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 3240
Size: 1009 2231
BsiHKAI G_wGCw'C
Cuts at: 0 1139 1406 1937 1963 3240
Size: 1139 267 531 26 1277
Fragments arranged by size:
1277 1139 531 267 26
BsiWI C'GTAC_G
Cuts at: 0 1863 3240
Size: 1863 1377
BsmI GAATG_Cn'
Cuts at: 0 1491 3185 3240
Size: 1491 1694 55
Fragments arranged by size:
1694 1491 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3193 3240
Size: 3193 47
Bsp1286I G_dGCh'C
Cuts at: 0 404 694 1139 1201 1406 1602 1641 1666
Size: 404 290 445 62 205 196 39 25
Cuts at: 1666 1937 1963 2091 2593 2823 2910 3079 3240
Size: 271 26 128 502 230 87 169 161
Fragments arranged by size:
502 445 404 290 271 230 205 196
169 161 128 87 62 39 26 25
BspEI T'CCGG_A
Cuts at: 0 1379 3240
Size: 1379 1861
BspHI T'CATG_A
Cuts at: 0 1049 3240
Size: 1049 2191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 502 2057 2291 3240
Size: 502 1555 234 949
Fragments arranged by size:
1555 949 502 234
BsrBI CCG'CTC
Cuts at: 0 639 707 2207 2238 2598 3087 3240
Size: 639 68 1500 31 360 489 153
Fragments arranged by size:
1500 639 489 360 153 68 31
BsrDI GCAATG_nn'
Cuts at: 0 504 746 1871 3240
Size: 504 242 1125 1369
Fragments arranged by size:
1369 1125 504 242
BsrFI r'CCGG_y
Cuts at: 0 2744 3240
Size: 2744 496
BssSI C'ACGA_G
Cuts at: 0 2228 2364 3164 3240
Size: 2228 136 800 76
Fragments arranged by size:
2228 800 136 76
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1989 2953 3240
Size: 1989 964 287
Fragments arranged by size:
1989 964 287
BstBI TT'CG_AA
Cuts at: 0 615 3240
Size: 615 2625
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1536 2691 3240
Size: 1536 1155 549
Fragments arranged by size:
1536 1155 549
BstYI r'GATC_y
Cuts at: 0 33 791 1613 1967 2922 3009 3240
Size: 33 758 822 354 955 87 231
Fragments arranged by size:
955 822 758 354 231 87 33
Bsu36I CC'TnA_GG
Cuts at: 0 244 2585 3240
Size: 244 2341 655
Fragments arranged by size:
2341 655 244
BtgI C'CryG_G
Cuts at: 0 699 842 1497 2684 3240
Size: 699 143 655 1187 556
Fragments arranged by size:
1187 699 655 556 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1213 2966 3240
Size: 1213 1753 274
Fragments arranged by size:
1753 1213 274
BtsI GCAGTG_nn'
Cuts at: 0 2172 2378 2821 3108 3240
Size: 2172 206 443 287 132
Fragments arranged by size:
2172 443 287 206 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 729 764 3240
Size: 729 35 2476
Fragments arranged by size:
2476 729 35
DraIII CAC_nnn'GTG
Cuts at: 0 1032 3240
Size: 1032 2208
EaeI y'GGCC_r
Cuts at: 0 140 640 650 702 1671 2861 3240
Size: 140 500 10 52 969 1190 379
Fragments arranged by size:
1190 969 500 379 140 52 10
EarI CTCTTCn'nnn_
Cuts at: 0 29 412 617 857 1066 1076 2147 2837
Size: 29 383 205 240 209 10 1071 690
Cuts at: 2837 3077 3240
Size: 240 163
Fragments arranged by size:
1071 690 383 240 240 209 205 163
29 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3140 3240
Size: 3140 100
EcoICRI GAG'CTC
Cuts at: 0 1137 3240
Size: 1137 2103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 601 1063 1609 1774 1820 2806 2848
Size: 297 304 462 546 165 46 986 42
Cuts at: 2848 3240
Size: 392
Fragments arranged by size:
986 546 462 392 304 297 165 46
42
EcoO109I rG'GnC_Cy
Cuts at: 0 8 19 1113 1191 1264 1521 1598 1662
Size: 8 11 1094 78 73 257 77 64
Cuts at: 1662 1663 2196 2589 2590 2898 3240
Size: 1 533 393 1 308 342
Fragments arranged by size:
1094 533 393 342 308 257 78 77
73 64 11 8 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1683 1830 3240
Size: 1683 147 1410
Fragments arranged by size:
1683 1410 147
FspI TGC'GCA
Cuts at: 0 1993 3240
Size: 1993 1247
FspAI rTGC'GCAy
Cuts at: 0 1993 3240
Size: 1993 1247
HaeII r_GCGC'y
Cuts at: 0 1518 1743 2742 3240
Size: 1518 225 999 498
Fragments arranged by size:
1518 999 498 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1543 1575 2099 2131 2425 2457 3240
Size: 1543 32 524 32 294 32 783
Fragments arranged by size:
1543 783 524 294 32 32 32
KpnI G_GTAC'C
Cuts at: 0 284 3240
Size: 284 2956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 3240
Size: 689 2551
MslI CAynn'nnrTG
Cuts at: 0 74 148 436 761 1072 2566 3240
Size: 74 74 288 325 311 1494 674
Fragments arranged by size:
1494 674 325 311 288 74 74
MspA1I CmG'CkG
Cuts at: 0 264 518 922 1587 2354 2472 2545 2706
Size: 264 254 404 665 767 118 73 161
Cuts at: 2706 2859 2893 3240
Size: 153 34 347
Fragments arranged by size:
767 665 404 347 264 254 161 153
118 73 34
NaeI GCC'GGC
Cuts at: 0 2746 3240
Size: 2746 494
NgoMIV G'CCGG_C
Cuts at: 0 2744 3240
Size: 2744 496
NspI r_CATG'y
Cuts at: 0 153 825 2000 2716 3240
Size: 153 672 1175 716 524
Fragments arranged by size:
1175 716 672 524 153
PasI CC'CwG_GG
Cuts at: 0 777 2192 2868 2903 3240
Size: 777 1415 676 35 337
Fragments arranged by size:
1415 777 676 337 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 121 1233 1654 3240
Size: 121 1112 421 1586
Fragments arranged by size:
1586 1112 421 121
PfoI T'CCnGG_A
Cuts at: 0 632 1790 2798 3191 3240
Size: 632 1158 1008 393 49
Fragments arranged by size:
1158 1008 632 393 49
PpuMI rG'GwC_Cy
Cuts at: 0 1264 1521 3240
Size: 1264 257 1719
Fragments arranged by size:
1719 1264 257
PspOMI G'GGCC_C
Cuts at: 0 1598 1637 1662 2589 2906 3240
Size: 1598 39 25 927 317 334
Fragments arranged by size:
1598 927 334 317 39 25
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2681 2713 3240
Size: 2681 32 527
Fragments arranged by size:
2681 527 32
PstI C_TGCA'G
Cuts at: 0 33 259 962 1309 1490 1610 1853 2249
Size: 33 226 703 347 181 120 243 396
Cuts at: 2249 2428 2705 2726 3240
Size: 179 277 21 514
Fragments arranged by size:
703 514 396 347 277 243 226 181
179 120 33 21
PvuII CAG'CTG
Cuts at: 0 264 922 1587 2472 2545 2859 2893 3240
Size: 264 658 665 885 73 314 34 347
Fragments arranged by size:
885 665 658 347 314 264 73 34
SacI G_AGCT'C
Cuts at: 0 1139 3240
Size: 1139 2101
SapI GCTCTTCn'nnn_
Cuts at: 0 1076 3077 3240
Size: 1076 2001 163
Fragments arranged by size:
2001 1076 163
SfcI C'TryA_G
Cuts at: 0 29 255 873 958 1305 1486 1606 1849
Size: 29 226 618 85 347 181 120 243
Cuts at: 1849 2245 2424 2536 2701 2722 3224 3240
Size: 396 179 112 165 21 502 16
Fragments arranged by size:
618 502 396 347 243 226 181 179
165 120 112 85 29 21 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1670 2905 3240
Size: 1670 1235 335
Fragments arranged by size:
1670 1235 335
SmaI CCC'GGG
Cuts at: 0 286 352 1948 2818 3240
Size: 286 66 1596 870 422
Fragments arranged by size:
1596 870 422 286 66
SmlI C'TyrA_G
Cuts at: 0 1405 2218 2964 3240
Size: 1405 813 746 276
Fragments arranged by size:
1405 813 746 276
SphI G_CATG'C
Cuts at: 0 153 3240
Size: 153 3087
SrfI GCCC'GGGC
Cuts at: 0 2818 3240
Size: 2818 422
StuI AGG'CCT
Cuts at: 0 1324 3240
Size: 1324 1916
StyI C'CwwG_G
Cuts at: 0 12 377 470 545 2339 3240
Size: 12 365 93 75 1794 901
Fragments arranged by size:
1794 901 365 93 75 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1225 2893 3068 3240
Size: 1225 1668 175 172
Fragments arranged by size:
1668 1225 175 172
TatI w'GTAC_w
Cuts at: 0 1776 1808 2960 3240
Size: 1776 32 1152 280
Fragments arranged by size:
1776 1152 280 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1267 1299 1927 1959 2596 2628 3240
Size: 1267 32 628 32 637 32 612
Fragments arranged by size:
1267 637 628 612 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 142 2262 3240
Size: 142 2120 978
Fragments arranged by size:
2120 978 142
XmaI C'CCGG_G
Cuts at: 0 284 350 1946 2816 3240
Size: 284 66 1596 870 424
Fragments arranged by size:
1596 870 424 284 66
Enzymes that do cut:
Acc65I AcuI AleI AlfI AloI AlwNI ApaI ApaLI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BciVI
BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I KpnI
MmeI MslI MspA1I NaeI NgoMIV NspI PasI PflMI
PfoI PpuMI PspOMI PsrI PstI PvuII SacI SapI
SfcI SfiI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI ApoI AscI AseI AsiSI AvrII BbeI BcgI
BfrBI BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI
BsiEI BsrGI BssHII BstEII BstZ17I ClaI DraI DrdI
EagI EcoNI EcoRI EcoRV FseI HincII HindIII HpaI
KasI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI