Restriction map for
pF1KSDA0649
(Linear) (Six-base) MAPSORT of: KIAA0649.seq Check: 7924 from: 1 to: 3627
KIAA0649.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 965 1369 2060 2269 3291 3339 3561
Size: 442 523 404 691 209 1022 48 222
Cuts at: 3561 3627
Size: 66
Fragments arranged by size:
1022 691 523 442 404 222 209 66
48
AfeI AGC'GCT
Cuts at: 0 1026 3627
Size: 1026 2601
AhdI GACnn_n'nnGTC
Cuts at: 0 3183 3523 3627
Size: 3183 340 104
Fragments arranged by size:
3183 340 104
AleI CACnn'nnGTG
Cuts at: 0 536 3627
Size: 536 3091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 246 987 2366 3627
Size: 246 741 1379 1261
Fragments arranged by size:
1379 1261 741 246
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1537 1569 3627
Size: 1537 32 2058
Fragments arranged by size:
2058 1537 32
AlwNI CAG_nnn'CTG
Cuts at: 0 699 837 3627
Size: 699 138 2790
Fragments arranged by size:
2790 699 138
ApaI G_GGCC'C
Cuts at: 0 465 1966 2239 2449 2587 2611 3196 3340
Size: 465 1501 273 210 138 24 585 144
Cuts at: 3340 3627
Size: 287
Fragments arranged by size:
1501 585 465 287 273 210 144 138
24
ApaLI G'TGCA_C
Cuts at: 0 265 2664 3627
Size: 265 2399 963
Fragments arranged by size:
2399 963 265
ApoI r'AATT_y
Cuts at: 0 771 3627
Size: 771 2856
AvaI C'yCGr_G
Cuts at: 0 134 179 448 1246 2795 3009 3030 3200
Size: 134 45 269 798 1549 214 21 170
Cuts at: 3200 3480 3627
Size: 280 147
Fragments arranged by size:
1549 798 280 269 214 170 147 134
45 21
BamHI G'GATC_C
Cuts at: 0 607 2802 3627
Size: 607 2195 825
Fragments arranged by size:
2195 825 607
BanI G'GyrC_C
Cuts at: 0 184 1951 2015 2350 2808 2959 2974 3627
Size: 184 1767 64 335 458 151 15 653
Fragments arranged by size:
1767 653 458 335 184 151 64 15
BanII G_rGCy'C
Cuts at: 0 465 628 1381 1889 1966 2038 2141 2213
Size: 465 163 753 508 77 72 103 72
Cuts at: 2213 2239 2449 2587 2611 2780 2852 2896 3010
Size: 26 210 138 24 169 72 44 114
Cuts at: 3010 3057 3196 3340 3481 3487 3627
Size: 47 139 144 141 6 140
Fragments arranged by size:
753 508 465 210 169 163 144 141
140 139 138 114 103 77 72 72
72 47 44 26 24 6
BbeI G_GCGC'C
Cuts at: 0 2019 2978 3627
Size: 2019 959 649
Fragments arranged by size:
2019 959 649
BbsI GAAGACnn'nnnn_
Cuts at: 0 2048 3627
Size: 2048 1579
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 157 191 3627
Size: 157 34 3436
Fragments arranged by size:
3436 157 34
BglI GCCn_nnn'nGGC
Cuts at: 0 247 460 842 1633 1724 1933 3627
Size: 247 213 382 791 91 209 1694
Fragments arranged by size:
1694 791 382 247 213 209 91
BlpI GC'TnA_GC
Cuts at: 0 1436 1883 2906 3627
Size: 1436 447 1023 721
Fragments arranged by size:
1436 1023 721 447
Bme1580I G_kGCm'C
Cuts at: 0 187 269 465 543 1954 1966 2239 2449
Size: 187 82 196 78 1411 12 273 210
Cuts at: 2449 2587 2611 2668 2746 2962 3196 3340 3627
Size: 138 24 57 78 216 234 144 287
Fragments arranged by size:
1411 287 273 234 216 210 196 187
144 138 82 78 78 57 24 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 2303 3627
Size: 2303 1324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 19 1068 1355 2536 3071 3099 3627
Size: 5 14 1049 287 1181 535 28 528
Fragments arranged by size:
1181 1049 535 528 287 28 14 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2104 3627
Size: 2104 1523
BsaI GGTCTCn'nnnn_
Cuts at: 0 304 715 1243 1898 2385 3627
Size: 304 411 528 655 487 1242
Fragments arranged by size:
1242 655 528 487 411 304
BsaBI GATnn'nnATC
Cuts at: 0 148 3627
Size: 148 3479
BsaHI Gr'CG_yC
Cuts at: 0 239 845 1066 1075 2016 2975 3161 3551
Size: 239 606 221 9 941 959 186 390
Cuts at: 3551 3566 3627
Size: 15 61
Fragments arranged by size:
959 941 606 390 239 221 186 61
15 9
BsaWI w'CCGG_w
Cuts at: 0 1613 2213 2805 3627
Size: 1613 600 592 822
Fragments arranged by size:
1613 822 600 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 610 640 1347 1377 1561 1591
Size: 106 30 474 30 707 30 184 30
Cuts at: 1591 3627
Size: 2036
Fragments arranged by size:
2036 707 474 184 106 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 415 1120 1201 2403 3489 3627
Size: 343 72 705 81 1202 1086 138
Fragments arranged by size:
1202 1086 705 343 138 81 72
BseYI C'CCAG_C
Cuts at: 0 464 479 840 1267 1767 2202 2448 2455
Size: 464 15 361 427 500 435 246 7
Cuts at: 2455 2610 2941 3025 3109 3211 3251 3376 3627
Size: 155 331 84 84 102 40 125 251
Fragments arranged by size:
500 464 435 427 361 331 251 246
155 125 102 84 84 40 15 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 494 1469 2151 2172 2652 3627
Size: 160 334 975 682 21 480 975
Fragments arranged by size:
975 975 682 480 334 160 21
BsiEI CG_ry'CG
Cuts at: 0 3066 3627
Size: 3066 561
BsiHKAI G_wGCw'C
Cuts at: 0 269 2038 2668 2739 2824 3481 3627
Size: 269 1769 630 71 85 657 146
Fragments arranged by size:
1769 657 630 269 146 85 71
Bsp1286I G_dGCh'C
Cuts at: 0 187 269 465 543 628 1381 1889 1954
Size: 187 82 196 78 85 753 508 65
Cuts at: 1954 1966 2038 2141 2213 2239 2449 2587 2611
Size: 12 72 103 72 26 210 138 24
Cuts at: 2611 2668 2739 2746 2780 2824 2852 2896 2962
Size: 57 71 7 34 44 28 44 66
Cuts at: 2962 3010 3057 3196 3340 3481 3487 3627
Size: 48 47 139 144 141 6 140
Fragments arranged by size:
753 508 210 196 187 144 141 140
139 138 103 85 82 78 72 72
71 66 65 57 48 47 44 44
34 28 26 24 12 7 6
BspEI T'CCGG_A
Cuts at: 0 1613 2213 3627
Size: 1613 600 1414
Fragments arranged by size:
1613 1414 600
BspHI T'CATG_A
Cuts at: 0 8 1049 3627
Size: 8 1041 2578
Fragments arranged by size:
2578 1041 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1169 1916 3627
Size: 1169 747 1711
Fragments arranged by size:
1711 1169 747
BsrBI CCG'CTC
Cuts at: 0 1559 2593 3037 3627
Size: 1559 1034 444 590
Fragments arranged by size:
1559 1034 590 444
BsrFI r'CCGG_y
Cuts at: 0 246 454 525 960 1485 1723 2643 3627
Size: 246 208 71 435 525 238 920 984
Fragments arranged by size:
984 920 525 435 246 238 208 71
BssHII G'CGCG_C
Cuts at: 0 198 3627
Size: 198 3429
BssSI C'ACGA_G
Cuts at: 0 1413 2897 3627
Size: 1413 1484 730
Fragments arranged by size:
1484 1413 730
BstAPI GCAn_nnn'nTGC
Cuts at: 0 837 3627
Size: 837 2790
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1299 3627
Size: 1299 2328
BstYI r'GATC_y
Cuts at: 0 607 2802 3627
Size: 607 2195 825
Fragments arranged by size:
2195 825 607
Bsu36I CC'TnA_GG
Cuts at: 0 913 3508 3627
Size: 913 2595 119
Fragments arranged by size:
2595 913 119
BtgI C'CryG_G
Cuts at: 0 320 383 1298 1580 3115 3590 3627
Size: 320 63 915 282 1535 475 37
Fragments arranged by size:
1535 915 475 320 282 63 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 206 668 1604 3627
Size: 206 462 936 2023
Fragments arranged by size:
2023 936 462 206
BtsI GCAGTG_nn'
Cuts at: 0 543 1230 1509 1762 3152 3567 3627
Size: 543 687 279 253 1390 415 60
Fragments arranged by size:
1390 687 543 415 279 253 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 266 301 2577 2612 3627
Size: 266 35 2276 35 1015
Fragments arranged by size:
2276 1015 266 35 35
EaeI y'GGCC_r
Cuts at: 0 1286 1655 1728 1797 1890 2702 3430 3627
Size: 1286 369 73 69 93 812 728 197
Fragments arranged by size:
1286 812 728 369 197 93 73 69
EarI CTCTTCn'nnn_
Cuts at: 0 703 1537 2119 2245 3262 3310 3627
Size: 703 834 582 126 1017 48 317
Fragments arranged by size:
1017 834 703 582 317 126 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 515 618 697 765 1438 2391 2551 2829
Size: 515 103 79 68 673 953 160 278
Cuts at: 2829 3627
Size: 798
Fragments arranged by size:
953 798 673 515 278 160 103 79
68
EcoICRI GAG'CTC
Cuts at: 0 2036 3479 3627
Size: 2036 1443 148
Fragments arranged by size:
2036 1443 148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 19 442 965 1068 1355 1369 2060
Size: 5 14 423 523 103 287 14 691
Cuts at: 2060 2269 2536 3071 3099 3291 3339 3561 3627
Size: 209 267 535 28 192 48 222 66
Fragments arranged by size:
691 535 523 423 287 267 222 209
192 103 66 48 28 14 14 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 578 1310 2795 3627
Size: 578 732 1485 832
Fragments arranged by size:
1485 832 732 578
EcoO109I rG'GnC_Cy
Cuts at: 0 461 834 1158 1317 1511 1935 1962 1963
Size: 461 373 324 159 194 424 27 1
Cuts at: 1963 2073 2235 2290 2445 2583 2584 2607 2691
Size: 110 162 55 155 138 1 23 84
Cuts at: 2691 3192 3193 3204 3336 3337 3627
Size: 501 1 11 132 1 290
Fragments arranged by size:
501 461 424 373 324 290 194 162
159 155 138 132 110 84 55 27
23 11 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 3627
Size: 804 2823
HaeII r_GCGC'y
Cuts at: 0 134 1022 1028 1198 2019 2978 3627
Size: 134 888 6 170 821 959 649
Fragments arranged by size:
959 888 821 649 170 134 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 610 642 1561 1593 3169 3201
Size: 106 32 472 32 919 32 1576 32
Cuts at: 3201 3509 3541 3627
Size: 308 32 86
Fragments arranged by size:
1576 919 472 308 106 86 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 3528 3627
Size: 3528 99
KasI G'GCGC_C
Cuts at: 0 2015 2974 3627
Size: 2015 959 653
Fragments arranged by size:
2015 959 653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1200 3627
Size: 1103 97 2427
Fragments arranged by size:
2427 1103 97
MscI TGG'CCA
Cuts at: 0 1288 1657 1799 3432 3627
Size: 1288 369 142 1633 195
Fragments arranged by size:
1633 1288 369 195 142
MslI CAynn'nnrTG
Cuts at: 0 536 1978 3627
Size: 536 1442 1649
Fragments arranged by size:
1649 1442 536
MspA1I CmG'CkG
Cuts at: 0 1119 1128 2202 2749 2828 2937 2945 2956
Size: 1119 9 1074 547 79 109 8 11
Cuts at: 2956 3240 3627
Size: 284 387
Fragments arranged by size:
1119 1074 547 387 284 109 79 11
9 8
NaeI GCC'GGC
Cuts at: 0 248 456 962 1487 2645 3627
Size: 248 208 506 525 1158 982
Fragments arranged by size:
1158 982 525 506 248 208
NarI GG'CG_CC
Cuts at: 0 2016 2975 3627
Size: 2016 959 652
Fragments arranged by size:
2016 959 652
NcoI C'CATG_G
Cuts at: 0 320 1298 3627
Size: 320 978 2329
Fragments arranged by size:
2329 978 320
NgoMIV G'CCGG_C
Cuts at: 0 246 454 960 1485 2643 3627
Size: 246 208 506 525 1158 984
Fragments arranged by size:
1158 984 525 506 246 208
NspI r_CATG'y
Cuts at: 0 1983 3627
Size: 1983 1644
PasI CC'CwG_GG
Cuts at: 0 1686 1938 2141 2432 2442 3627
Size: 1686 252 203 291 10 1185
Fragments arranged by size:
1686 1185 291 252 203 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 2656 3116 3627
Size: 2656 460 511
Fragments arranged by size:
2656 511 460
PfoI T'CCnGG_A
Cuts at: 0 410 1387 1460 3627
Size: 410 977 73 2167
Fragments arranged by size:
2167 977 410 73
PpuMI rG'GwC_Cy
Cuts at: 0 1158 1511 2073 2290 2691 3627
Size: 1158 353 562 217 401 936
Fragments arranged by size:
1158 936 562 401 353 217
PshAI GACnn'nnGTC
Cuts at: 0 1307 3627
Size: 1307 2320
PspOMI G'GGCC_C
Cuts at: 0 461 1962 2235 2445 2583 2607 3192 3336
Size: 461 1501 273 210 138 24 585 144
Cuts at: 3336 3627
Size: 291
Fragments arranged by size:
1501 585 461 291 273 210 144 138
24
PstI C_TGCA'G
Cuts at: 0 164 1133 3335 3627
Size: 164 969 2202 292
Fragments arranged by size:
2202 969 292 164
PvuII CAG'CTG
Cuts at: 0 1119 1128 2202 2749 2937 2945 3627
Size: 1119 9 1074 547 188 8 682
Fragments arranged by size:
1119 1074 682 547 188 9 8
SacI G_AGCT'C
Cuts at: 0 2038 3481 3627
Size: 2038 1443 146
Fragments arranged by size:
2038 1443 146
SanDI GG'GwC_CC
Cuts at: 0 1511 2691 3627
Size: 1511 1180 936
Fragments arranged by size:
1511 1180 936
SapI GCTCTTCn'nnn_
Cuts at: 0 2245 3262 3627
Size: 2245 1017 365
Fragments arranged by size:
2245 1017 365
SfcI C'TryA_G
Cuts at: 0 72 160 338 1129 1493 3331 3627
Size: 72 88 178 791 364 1838 296
Fragments arranged by size:
1838 791 364 296 178 88 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 460 3627
Size: 460 3167
SfoI GGC'GCC
Cuts at: 0 2017 2976 3627
Size: 2017 959 651
Fragments arranged by size:
2017 959 651
SmaI CCC'GGG
Cuts at: 0 136 450 3032 3202 3627
Size: 136 314 2582 170 425
Fragments arranged by size:
2582 425 314 170 136
SmlI C'TyrA_G
Cuts at: 0 2119 3627
Size: 2119 1508
StuI AGG'CCT
Cuts at: 0 52 3627
Size: 52 3575
StyI C'CwwG_G
Cuts at: 0 320 920 1298 2546 2843 3554 3627
Size: 320 600 378 1248 297 711 73
Fragments arranged by size:
1248 711 600 378 320 297 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1281 3108 3265 3627
Size: 1281 1827 157 362
Fragments arranged by size:
1827 1281 362 157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 733 1296 1935 3627
Size: 733 563 639 1692
Fragments arranged by size:
1692 733 639 563
XmaI C'CCGG_G
Cuts at: 0 134 448 3030 3200 3627
Size: 134 314 2582 170 427
Fragments arranged by size:
2582 427 314 170 134
XmnI GAAnn'nnTTC
Cuts at: 0 695 2425 2711 3627
Size: 695 1730 286 916
Fragments arranged by size:
1730 916 695 286
Enzymes that do cut:
AcuI AfeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BcgI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SapI SfcI SfiI SfoI SmaI SmlI StuI StyI
TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI
BfrBI BglII BmgBI BmtI BplI Bpu10I BsaAI BsiWI
BsmI BsmBI BsrDI BsrGI BstBI BstEII BstZ17I ClaI
DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI ZraI