Restriction map for
pF1KSDA0645
(Linear) (Six-base) MAPSORT of: KIAA0645.seq Check: 1441 from: 1 to: 4716
KIAA0645.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1447 1625 4716
Size: 1447 178 3091
Fragments arranged by size:
3091 1447 178
AccI GT'mk_AC
Cuts at: 0 17 269 4716
Size: 17 252 4447
Fragments arranged by size:
4447 252 17
AclI AA'CG_TT
Cuts at: 0 2548 4716
Size: 2548 2168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2037 2587 2754 2783 2809 4645 4716
Size: 2037 550 167 29 26 1836 71
Fragments arranged by size:
2037 1836 550 167 71 29 26
AflII C'TTAA_G
Cuts at: 0 172 4716
Size: 172 4544
AflIII A'CryG_T
Cuts at: 0 370 4517 4716
Size: 370 4147 199
Fragments arranged by size:
4147 370 199
AhdI GACnn_n'nnGTC
Cuts at: 0 2037 2205 4716
Size: 2037 168 2511
Fragments arranged by size:
2511 2037 168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1465 4716
Size: 1465 3251
AlwNI CAG_nnn'CTG
Cuts at: 0 1490 2268 2648 3402 4716
Size: 1490 778 380 754 1314
Fragments arranged by size:
1490 1314 778 754 380
ApaI G_GGCC'C
Cuts at: 0 1425 3032 4716
Size: 1425 1607 1684
Fragments arranged by size:
1684 1607 1425
ApaLI G'TGCA_C
Cuts at: 0 1709 3817 4716
Size: 1709 2108 899
Fragments arranged by size:
2108 1709 899
ApoI r'AATT_y
Cuts at: 0 697 2692 4716
Size: 697 1995 2024
Fragments arranged by size:
2024 1995 697
AvaI C'yCGr_G
Cuts at: 0 1012 1327 1418 1661 4716
Size: 1012 315 91 243 3055
Fragments arranged by size:
3055 1012 315 243 91
AvrII C'CTAG_G
Cuts at: 0 1575 2113 4716
Size: 1575 538 2603
Fragments arranged by size:
2603 1575 538
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 467 500 3529 3562 4716
Size: 467 33 3029 33 1154
Fragments arranged by size:
3029 1154 467 33 33
BamHI G'GATC_C
Cuts at: 0 1947 3566 4716
Size: 1947 1619 1150
Fragments arranged by size:
1947 1619 1150
BanI G'GyrC_C
Cuts at: 0 3274 4716
Size: 3274 1442
BanII G_rGCy'C
Cuts at: 0 1425 3032 3172 3704 3815 4716
Size: 1425 1607 140 532 111 901
Fragments arranged by size:
1607 1425 901 532 140 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 761 2771 4716
Size: 761 2010 1945
Fragments arranged by size:
2010 1945 761
BbvCI CC'TCA_GC
Cuts at: 0 900 3087 4716
Size: 900 2187 1629
Fragments arranged by size:
2187 1629 900
BciVI GTATCCnnnnn_n'
Cuts at: 0 851 1642 1762 2618 4716
Size: 851 791 120 856 2098
Fragments arranged by size:
2098 856 851 791 120
BclI T'GATC_A
Cuts at: 0 1004 2579 4716
Size: 1004 1575 2137
Fragments arranged by size:
2137 1575 1004
BfrBI ATG'CAT
Cuts at: 0 3634 4701 4716
Size: 3634 1067 15
Fragments arranged by size:
3634 1067 15
BglI GCCn_nnn'nGGC
Cuts at: 0 3802 3861 4716
Size: 3802 59 855
Fragments arranged by size:
3802 855 59
BglII A'GATC_T
Cuts at: 0 1444 4716
Size: 1444 3272
BlpI GC'TnA_GC
Cuts at: 0 2086 2461 4716
Size: 2086 375 2255
Fragments arranged by size:
2255 2086 375
Bme1580I G_kGCm'C
Cuts at: 0 1425 1713 3032 3821 4218 4713 4716
Size: 1425 288 1319 789 397 495 3
Fragments arranged by size:
1425 1319 789 495 397 288 3
BmgBI CAC'GTC
Cuts at: 0 3984 4716
Size: 3984 732
BmrI ACTGGGnnnn_n'
Cuts at: 0 995 1419 3035 3061 3171 4153 4181 4311
Size: 995 424 1616 26 110 982 28 130
Cuts at: 4311 4557 4716
Size: 246 159
Fragments arranged by size:
1616 995 982 424 246 159 130 110
28 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2694 4716
Size: 2694 2022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1689 1693 1996 2144 2731 2817 3070
Size: 940 749 4 303 148 587 86 253
Cuts at: 3070 3363 4027 4569 4711 4716
Size: 293 664 542 142 5
Fragments arranged by size:
940 749 664 587 542 303 293 253
148 142 86 5 4
Bpu10I CC'TnA_GC
Cuts at: 0 900 3087 4716
Size: 900 2187 1629
Fragments arranged by size:
2187 1629 900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 866 1376 4237 4716
Size: 206 660 510 2861 479
Fragments arranged by size:
2861 660 510 479 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 231 4716
Size: 231 4485
BsaAI yAC'GTr
Cuts at: 0 3558 4716
Size: 3558 1158
BsaHI Gr'CG_yC
Cuts at: 0 1379 4716
Size: 1379 3337
BsaWI w'CCGG_w
Cuts at: 0 1876 4716
Size: 1876 2840
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 98 128 1555 1585 2509 2539 2707 2737
Size: 98 30 1427 30 924 30 168 30
Cuts at: 2737 3243 3273 4716
Size: 506 30 1443
Fragments arranged by size:
1443 1427 924 506 168 98 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1563 1950 2080 2527 2530 2728 3357 4543
Size: 1563 387 130 447 3 198 629 1186
Cuts at: 4543 4716
Size: 173
Fragments arranged by size:
1563 1186 629 447 387 198 173 130
3
BseYI C'CCAG_C
Cuts at: 0 2357 2684 4217 4716
Size: 2357 327 1533 499
Fragments arranged by size:
2357 1533 499 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1603 1757 2120 2365 2437 2915 3044
Size: 796 807 154 363 245 72 478 129
Cuts at: 3044 4035 4054 4716
Size: 991 19 662
Fragments arranged by size:
991 807 796 662 478 363 245 154
129 72 19
BsiEI CG_ry'CG
Cuts at: 0 3708 4716
Size: 3708 1008
BsiHKAI G_wGCw'C
Cuts at: 0 417 1161 1713 1788 3172 3815 3821 4271
Size: 417 744 552 75 1384 643 6 450
Cuts at: 4271 4716
Size: 445
Fragments arranged by size:
1384 744 643 552 450 445 417 75
6
BsmI GAATG_Cn'
Cuts at: 0 2957 3042 4053 4316 4716
Size: 2957 85 1011 263 400
Fragments arranged by size:
2957 1011 400 263 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2395 2682 4716
Size: 2395 287 2034
Fragments arranged by size:
2395 2034 287
Bsp1286I G_dGCh'C
Cuts at: 0 417 1161 1425 1713 1788 3032 3172 3704
Size: 417 744 264 288 75 1244 140 532
Cuts at: 3704 3815 3821 4218 4271 4713 4716
Size: 111 6 397 53 442 3
Fragments arranged by size:
1244 744 532 442 417 397 288 264
140 111 75 53 6 3
BspEI T'CCGG_A
Cuts at: 0 1876 4716
Size: 1876 2840
BspHI T'CATG_A
Cuts at: 0 639 1046 1989 4716
Size: 639 407 943 2727
Fragments arranged by size:
2727 943 639 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 150 1447 1625 2092 2352 4716
Size: 150 1297 178 467 260 2364
Fragments arranged by size:
2364 1297 467 260 178 150
BsrBI CCG'CTC
Cuts at: 0 4533 4716
Size: 4533 183
BsrDI GCAATG_nn'
Cuts at: 0 4331 4716
Size: 4331 385
BsrFI r'CCGG_y
Cuts at: 0 2755 3860 4001 4661 4716
Size: 2755 1105 141 660 55
Fragments arranged by size:
2755 1105 660 141 55
BsrGI T'GTAC_A
Cuts at: 0 4353 4716
Size: 4353 363
BssSI C'ACGA_G
Cuts at: 0 3814 4716
Size: 3814 902
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3606 4716
Size: 3606 1110
BstEII G'GTnAC_C
Cuts at: 0 4083 4716
Size: 4083 633
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3219 3331 4716
Size: 3219 112 1385
Fragments arranged by size:
3219 1385 112
BstYI r'GATC_y
Cuts at: 0 1444 1892 1947 2627 3443 3566 4716
Size: 1444 448 55 680 816 123 1150
Fragments arranged by size:
1444 1150 816 680 448 123 55
Bsu36I CC'TnA_GG
Cuts at: 0 275 2510 4716
Size: 275 2235 2206
Fragments arranged by size:
2235 2206 275
BtgI C'CryG_G
Cuts at: 0 330 3305 4029 4716
Size: 330 2975 724 687
Fragments arranged by size:
2975 724 687 330
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1196 2956 3595 4716
Size: 1196 1760 639 1121
Fragments arranged by size:
1760 1196 1121 639
BtsI GCAGTG_nn'
Cuts at: 0 60 1528 1740 2865 4180 4716
Size: 60 1468 212 1125 1315 536
Fragments arranged by size:
1468 1315 1125 536 212 60
DraIII CAC_nnn'GTG
Cuts at: 0 2559 4716
Size: 2559 2157
EaeI y'GGCC_r
Cuts at: 0 327 1311 3584 3713 3862 4716
Size: 327 984 2273 129 149 854
Fragments arranged by size:
2273 984 854 327 149 129
EarI CTCTTCn'nnn_
Cuts at: 0 783 835 2366 3160 3608 3803 4105 4228
Size: 783 52 1531 794 448 195 302 123
Cuts at: 4228 4324 4716
Size: 96 392
Fragments arranged by size:
1531 794 783 448 392 302 195 123
96 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1936 3162 3666 4503 4716
Size: 1936 1226 504 837 213
Fragments arranged by size:
1936 1226 837 504 213
EcoICRI GAG'CTC
Cuts at: 0 3170 3813 4716
Size: 3170 643 903
Fragments arranged by size:
3170 903 643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1689 1693 1996 2037 2144 2587 2731
Size: 940 749 4 303 41 107 443 144
Cuts at: 2731 2754 2783 2809 2817 3070 3363 4027 4569
Size: 23 29 26 8 253 293 664 542
Cuts at: 4569 4645 4711 4716
Size: 76 66 5
Fragments arranged by size:
940 749 664 542 443 303 293 253
144 107 76 66 41 29 26 23
8 5 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4321 4716
Size: 4321 395
EcoO109I rG'GnC_Cy
Cuts at: 0 2117 2265 2801 2888 3501 4171 4716
Size: 2117 148 536 87 613 670 545
Fragments arranged by size:
2117 670 613 545 536 148 87
EcoRI G'AATT_C
Cuts at: 0 2692 4716
Size: 2692 2024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 60 4716
Size: 60 4656
HaeII r_GCGC'y
Cuts at: 0 1848 4584 4592 4716
Size: 1848 2736 8 124
Fragments arranged by size:
2736 1848 124 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 228 236 260 670 702 1455 1486
Size: 204 24 8 24 410 32 753 31
Cuts at: 1486 1487 1518 1555 1587 1871 1903 2023 2055
Size: 1 31 37 32 284 32 120 32
Cuts at: 2055 2191 2223 3376 3408 4716
Size: 136 32 1153 32 1308
Fragments arranged by size:
1308 1153 753 410 284 204 136 120
37 32 32 32 32 32 32 31
31 24 24 8 1
HincII GTy'rAC
Cuts at: 0 3056 3981 4716
Size: 3056 925 735
Fragments arranged by size:
3056 925 735
HindIII A'AGCT_T
Cuts at: 0 100 1825 4716
Size: 100 1725 2891
Fragments arranged by size:
2891 1725 100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1859 1975 2172 3086 3248 4716
Size: 1859 116 197 914 162 1468
Fragments arranged by size:
1859 1468 914 197 162 116
MscI TGG'CCA
Cuts at: 0 3586 3715 4716
Size: 3586 129 1001
Fragments arranged by size:
3586 1001 129
MslI CAynn'nnrTG
Cuts at: 0 1597 1625 3217 3262 4706 4716
Size: 1597 28 1592 45 1444 10
Fragments arranged by size:
1597 1592 1444 45 28 10
MspA1I CmG'CkG
Cuts at: 0 1065 1937 2460 3151 3402 3483 4019 4244
Size: 1065 872 523 691 251 81 536 225
Cuts at: 4244 4443 4479 4716
Size: 199 36 237
Fragments arranged by size:
1065 872 691 536 523 251 237 225
199 81 36
NaeI GCC'GGC
Cuts at: 0 2757 3862 4716
Size: 2757 1105 854
Fragments arranged by size:
2757 1105 854
NcoI C'CATG_G
Cuts at: 0 3305 4029 4716
Size: 3305 724 687
Fragments arranged by size:
3305 724 687
NdeI CA'TA_TG
Cuts at: 0 3289 4716
Size: 3289 1427
NgoMIV G'CCGG_C
Cuts at: 0 2755 3860 4716
Size: 2755 1105 856
Fragments arranged by size:
2755 1105 856
NsiI A_TGCA'T
Cuts at: 0 3636 4703 4716
Size: 3636 1067 13
Fragments arranged by size:
3636 1067 13
NspI r_CATG'y
Cuts at: 0 374 1126 1756 2040 3634 4521 4705 4716
Size: 374 752 630 284 1594 887 184 11
Fragments arranged by size:
1594 887 752 630 374 284 184 11
PciI A'CATG_T
Cuts at: 0 370 4517 4716
Size: 370 4147 199
Fragments arranged by size:
4147 370 199
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 3306 3737 4577 4716
Size: 393 2913 431 840 139
Fragments arranged by size:
2913 840 431 393 139
PfoI T'CCnGG_A
Cuts at: 0 1670 2918 3446 4322 4716
Size: 1670 1248 528 876 394
Fragments arranged by size:
1670 1248 876 528 394
PmlI CAC'GTG
Cuts at: 0 3558 4716
Size: 3558 1158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3075 3107 4716
Size: 3075 32 1609
Fragments arranged by size:
3075 1609 32
PpuMI rG'GwC_Cy
Cuts at: 0 2117 2801 4716
Size: 2117 684 1915
Fragments arranged by size:
2117 1915 684
PshAI GACnn'nnGTC
Cuts at: 0 652 3475 4716
Size: 652 2823 1241
Fragments arranged by size:
2823 1241 652
PspOMI G'GGCC_C
Cuts at: 0 1421 3028 4716
Size: 1421 1607 1688
Fragments arranged by size:
1688 1607 1421
PstI C_TGCA'G
Cuts at: 0 1444 1968 2273 4442 4716
Size: 1444 524 305 2169 274
Fragments arranged by size:
2169 1444 524 305 274
PvuII CAG'CTG
Cuts at: 0 3151 3402 3483 4019 4244 4443 4716
Size: 3151 251 81 536 225 199 273
Fragments arranged by size:
3151 536 273 251 225 199 81
SacI G_AGCT'C
Cuts at: 0 3172 3815 4716
Size: 3172 643 901
Fragments arranged by size:
3172 901 643
SapI GCTCTTCn'nnn_
Cuts at: 0 3160 3608 3803 4228 4716
Size: 3160 448 195 425 488
Fragments arranged by size:
3160 488 448 425 195
ScaI AGT'ACT
Cuts at: 0 4097 4716
Size: 4097 619
SexAI A'CCwGG_T
Cuts at: 0 1761 4716
Size: 1761 2955
SfcI C'TryA_G
Cuts at: 0 633 1440 1964 2269 2478 2640 4438 4716
Size: 633 807 524 305 209 162 1798 278
Fragments arranged by size:
1798 807 633 524 305 278 209 162
SmaI CCC'GGG
Cuts at: 0 1014 1420 4716
Size: 1014 406 3296
Fragments arranged by size:
3296 1014 406
SmlI C'TyrA_G
Cuts at: 0 172 221 881 1391 1661 4252 4716
Size: 172 49 660 510 270 2591 464
Fragments arranged by size:
2591 660 510 464 270 172 49
SphI G_CATG'C
Cuts at: 0 4705 4716
Size: 4705 11
SspI AAT'ATT
Cuts at: 0 4366 4716
Size: 4366 350
StuI AGG'CCT
Cuts at: 0 2489 4716
Size: 2489 2227
StyI C'CwwG_G
Cuts at: 0 1575 1643 2113 3305 4029 4113 4716
Size: 1575 68 470 1192 724 84 603
Fragments arranged by size:
1575 1192 724 603 470 84 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 400 995 1009 2676 3722 4716
Size: 400 595 14 1667 1046 994
Fragments arranged by size:
1667 1046 994 595 400 14
TatI w'GTAC_w
Cuts at: 0 2717 4095 4353 4716
Size: 2717 1378 258 363
Fragments arranged by size:
2717 1378 363 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1073 1084 1105 1116 1999 2031 2707 2739
Size: 1073 11 21 11 883 32 676 32
Cuts at: 2739 4716
Size: 1977
Fragments arranged by size:
1977 1073 883 676 32 32 21 11
11
XbaI T'CTAG_A
Cuts at: 0 664 2136 4716
Size: 664 1472 2580
Fragments arranged by size:
2580 1472 664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3045 3264 4716
Size: 3045 219 1452
Fragments arranged by size:
3045 1452 219
XhoI C'TCGA_G
Cuts at: 0 1661 4716
Size: 1661 3055
XmaI C'CCGG_G
Cuts at: 0 1012 1418 4716
Size: 1012 406 3298
Fragments arranged by size:
3298 1012 406
XmnI GAAnn'nnTTC
Cuts at: 0 592 4519 4630 4716
Size: 592 3927 111 86
Fragments arranged by size:
3927 592 111 86
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AhdI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SapI ScaI SexAI SfcI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AleI AloI AscI AseI
AsiSI BbeI BcgI BmtI BsaBI BsiWI BssHII BstBI
BstZ17I ClaI CspCI DraI DrdI EagI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MluI NarI
NheI NotI NruI PacI PasI PmeI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I
ZraI