(Linear) (Six-base) MAPSORT of: KIAA0642.seq Check: 5624 from: 1 to: 3960 KIAA0642.orf With 182 enzymes: SgfI * February 28, 2007 11:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1943 3960 Size: 1943 2017 AatII G_ACGT'C Cuts at: 0 822 3960 Size: 822 3138 AccI GT'mk_AC Cuts at: 0 1314 3960 Size: 1314 2646 Acc65I G'GTAC_C Cuts at: 0 243 3052 3960 Size: 243 2809 908 Fragments arranged by size: 2809 908 243 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 161 965 1196 1307 1370 2128 2224 2971 Size: 161 804 231 111 63 758 96 747 Cuts at: 2971 3032 3448 3960 Size: 61 416 512 Fragments arranged by size: 804 758 747 512 416 231 161 111 96 63 61 AhdI GACnn_n'nnGTC Cuts at: 0 1221 3960 Size: 1221 2739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 478 2483 3960 Size: 478 2005 1477 Fragments arranged by size: 2005 1477 478 AlwNI CAG_nnn'CTG Cuts at: 0 21 1371 1507 1935 2125 2224 3687 3960 Size: 21 1350 136 428 190 99 1463 273 Fragments arranged by size: 1463 1350 428 273 190 136 99 21 ApaI G_GGCC'C Cuts at: 0 2616 3960 Size: 2616 1344 ApaLI G'TGCA_C Cuts at: 0 1577 3960 Size: 1577 2383 ApoI r'AATT_y Cuts at: 0 196 606 649 1694 2363 3189 3330 3960 Size: 196 410 43 1045 669 826 141 630 Fragments arranged by size: 1045 826 669 630 410 196 141 43 AvaI C'yCGr_G Cuts at: 0 2615 3960 Size: 2615 1345 AvrII C'CTAG_G Cuts at: 0 3325 3960 Size: 3325 635 BanI G'GyrC_C Cuts at: 0 243 2957 3052 3960 Size: 243 2714 95 908 Fragments arranged by size: 2714 908 243 95 BanII G_rGCy'C Cuts at: 0 51 348 2616 3150 3205 3960 Size: 51 297 2268 534 55 755 Fragments arranged by size: 2268 755 534 297 55 51 BbeI G_GCGC'C Cuts at: 0 2961 3960 Size: 2961 999 BbsI GAAGACnn'nnnn_ Cuts at: 0 2000 2355 2532 3960 Size: 2000 355 177 1428 Fragments arranged by size: 2000 1428 355 177 BclI T'GATC_A Cuts at: 0 2076 3960 Size: 2076 1884 BfrBI ATG'CAT Cuts at: 0 465 1650 3910 3960 Size: 465 1185 2260 50 Fragments arranged by size: 2260 1185 465 50 BglI GCCn_nnn'nGGC Cuts at: 0 39 3960 Size: 39 3921 Bme1580I G_kGCm'C Cuts at: 0 1331 1581 2616 3960 Size: 1331 250 1035 1344 Fragments arranged by size: 1344 1331 1035 250 BmrI ACTGGGnnnn_n' Cuts at: 0 3878 3960 Size: 3878 82 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1083 3960 Size: 1083 2877 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 196 995 1826 2743 3091 3960 Size: 74 122 799 831 917 348 869 Fragments arranged by size: 917 869 831 799 348 122 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3658 3960 Size: 3658 302 BsaI GGTCTCn'nnnn_ Cuts at: 0 594 1528 3960 Size: 594 934 2432 Fragments arranged by size: 2432 934 594 BsaBI GATnn'nnATC Cuts at: 0 1599 3960 Size: 1599 2361 BsaHI Gr'CG_yC Cuts at: 0 819 2958 3960 Size: 819 2139 1002 Fragments arranged by size: 2139 1002 819 BsaWI w'CCGG_w Cuts at: 0 2780 3960 Size: 2780 1180 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 63 93 320 350 1383 1413 3204 3234 Size: 63 30 227 30 1033 30 1791 30 Cuts at: 3234 3960 Size: 726 Fragments arranged by size: 1791 1033 726 227 63 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 71 335 338 341 461 1075 1306 1391 Size: 71 264 3 3 120 614 231 85 Cuts at: 1391 1850 2553 2611 2662 2686 3960 Size: 459 703 58 51 24 1274 Fragments arranged by size: 1274 703 614 459 264 231 120 85 71 58 51 24 3 3 BseYI C'CCAG_C Cuts at: 0 479 576 1330 2012 2633 3960 Size: 479 97 754 682 621 1327 Fragments arranged by size: 1327 754 682 621 479 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3157 3856 3960 Size: 3157 699 104 Fragments arranged by size: 3157 699 104 BsiHKAI G_wGCw'C Cuts at: 0 51 1069 1479 1581 1619 3150 3960 Size: 51 1018 410 102 38 1531 810 Fragments arranged by size: 1531 1018 810 410 102 51 38 BsmI GAATG_Cn' Cuts at: 0 979 3162 3960 Size: 979 2183 798 Fragments arranged by size: 2183 979 798 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3937 3960 Size: 3937 23 Bsp1286I G_dGCh'C Cuts at: 0 51 348 1069 1331 1479 1581 1619 2616 Size: 51 297 721 262 148 102 38 997 Cuts at: 2616 3150 3205 3960 Size: 534 55 755 Fragments arranged by size: 997 755 721 534 297 262 148 102 55 51 38 BspEI T'CCGG_A Cuts at: 0 2780 3960 Size: 2780 1180 BspHI T'CATG_A Cuts at: 0 1811 3960 Size: 1811 2149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1943 3179 3960 Size: 1943 1236 781 Fragments arranged by size: 1943 1236 781 BsrDI GCAATG_nn' Cuts at: 0 94 1664 3960 Size: 94 1570 2296 Fragments arranged by size: 2296 1570 94 BsrFI r'CCGG_y Cuts at: 0 2654 3389 3960 Size: 2654 735 571 Fragments arranged by size: 2654 735 571 BstAPI GCAn_nnn'nTGC Cuts at: 0 1935 3687 3960 Size: 1935 1752 273 Fragments arranged by size: 1935 1752 273 BstEII G'GTnAC_C Cuts at: 0 865 3960 Size: 865 3095 BstXI CCAn_nnnn'nTGG Cuts at: 0 1801 3960 Size: 1801 2159 BstYI r'GATC_y Cuts at: 0 1680 2193 3074 3879 3960 Size: 1680 513 881 805 81 Fragments arranged by size: 1680 881 805 513 81 Bsu36I CC'TnA_GG Cuts at: 0 1685 3960 Size: 1685 2275 BtsI GCAGTG_nn' Cuts at: 0 894 1497 3314 3478 3960 Size: 894 603 1817 164 482 Fragments arranged by size: 1817 894 603 482 164 DrdI GACnn_nn'nnGTC Cuts at: 0 770 3960 Size: 770 3190 EaeI y'GGCC_r Cuts at: 0 1450 3960 Size: 1450 2510 EarI CTCTTCn'nnn_ Cuts at: 0 1016 1088 1283 1409 1765 2280 2309 2315 Size: 1016 72 195 126 356 515 29 6 Cuts at: 2315 2429 2483 2528 2538 2543 2588 2663 2704 Size: 114 54 45 10 5 45 75 41 Cuts at: 2704 2942 2990 3071 3080 3960 Size: 238 48 81 9 880 Fragments arranged by size: 1016 880 515 356 238 195 126 114 81 75 72 54 48 45 45 41 29 10 9 6 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2577 3960 Size: 2577 1383 EcoICRI GAG'CTC Cuts at: 0 49 3148 3960 Size: 49 3099 812 Fragments arranged by size: 3099 812 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 74 161 196 965 995 1196 1307 1370 Size: 74 87 35 769 30 201 111 63 Cuts at: 1370 1826 2128 2224 2743 2971 3032 3091 3448 Size: 456 302 96 519 228 61 59 357 Cuts at: 3448 3960 Size: 512 Fragments arranged by size: 769 519 512 456 357 302 228 201 111 96 87 74 63 61 59 35 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 214 3960 Size: 214 3746 EcoO109I rG'GnC_Cy Cuts at: 0 1702 2221 3322 3587 3960 Size: 1702 519 1101 265 373 Fragments arranged by size: 1702 1101 519 373 265 EcoRI G'AATT_C Cuts at: 0 3189 3330 3960 Size: 3189 141 630 Fragments arranged by size: 3189 630 141 EcoRV GAT'ATC Cuts at: 0 1809 3960 Size: 1809 2151 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2431 2536 3960 Size: 2431 105 1424 Fragments arranged by size: 2431 1424 105 HaeII r_GCGC'y Cuts at: 0 2961 3645 3960 Size: 2961 684 315 Fragments arranged by size: 2961 684 315 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1207 1239 1381 1413 2113 2145 2967 2999 Size: 1207 32 142 32 700 32 822 32 Cuts at: 2999 3887 3919 3960 Size: 888 32 41 Fragments arranged by size: 1207 888 822 700 142 41 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1788 2827 3960 Size: 1788 1039 1133 Fragments arranged by size: 1788 1133 1039 KasI G'GCGC_C Cuts at: 0 2957 3960 Size: 2957 1003 KpnI G_GTAC'C Cuts at: 0 247 3056 3960 Size: 247 2809 904 Fragments arranged by size: 2809 904 247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 802 1818 3191 3308 3929 3960 Size: 802 1016 1373 117 621 31 Fragments arranged by size: 1373 1016 802 621 117 31 MslI CAynn'nnrTG Cuts at: 0 1816 3960 Size: 1816 2144 MspA1I CmG'CkG Cuts at: 0 7 2454 2733 2814 3687 3960 Size: 7 2447 279 81 873 273 Fragments arranged by size: 2447 873 279 273 81 7 NarI GG'CG_CC Cuts at: 0 2958 3960 Size: 2958 1002 NdeI CA'TA_TG Cuts at: 0 1865 3960 Size: 1865 2095 NsiI A_TGCA'T Cuts at: 0 467 1652 3912 3960 Size: 467 1185 2260 48 Fragments arranged by size: 2260 1185 467 48 NspI r_CATG'y Cuts at: 0 558 2876 3960 Size: 558 2318 1084 Fragments arranged by size: 2318 1084 558 PflMI CCAn_nnn'nTGG Cuts at: 0 2125 3960 Size: 2125 1835 PfoI T'CCnGG_A Cuts at: 0 212 3960 Size: 212 3748 PpuMI rG'GwC_Cy Cuts at: 0 1702 3322 3960 Size: 1702 1620 638 Fragments arranged by size: 1702 1620 638 PspOMI G'GGCC_C Cuts at: 0 2612 3960 Size: 2612 1348 PstI C_TGCA'G Cuts at: 0 795 1940 3960 Size: 795 1145 2020 Fragments arranged by size: 2020 1145 795 PvuII CAG'CTG Cuts at: 0 2814 3687 3960 Size: 2814 873 273 Fragments arranged by size: 2814 873 273 SacI G_AGCT'C Cuts at: 0 51 3150 3960 Size: 51 3099 810 Fragments arranged by size: 3099 810 51 SapI GCTCTTCn'nnn_ Cuts at: 0 1016 2315 2942 2990 3960 Size: 1016 1299 627 48 970 Fragments arranged by size: 1299 1016 970 627 48 SexAI A'CCwGG_T Cuts at: 0 1200 3960 Size: 1200 2760 SfcI C'TryA_G Cuts at: 0 791 1936 2165 3374 3960 Size: 791 1145 229 1209 586 Fragments arranged by size: 1209 1145 791 586 229 SfoI GGC'GCC Cuts at: 0 2959 3960 Size: 2959 1001 SmaI CCC'GGG Cuts at: 0 2617 3960 Size: 2617 1343 SmlI C'TyrA_G Cuts at: 0 3637 3960 Size: 3637 323 StyI C'CwwG_G Cuts at: 0 594 3325 3614 3960 Size: 594 2731 289 346 Fragments arranged by size: 2731 594 346 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3922 3960 Size: 3922 38 TatI w'GTAC_w Cuts at: 0 557 999 3772 3960 Size: 557 442 2773 188 Fragments arranged by size: 2773 557 442 188 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 320 323 352 355 3739 3771 3960 Size: 320 3 29 3 3384 32 189 Fragments arranged by size: 3384 320 189 32 29 3 3 Tth111I GACn'n_nGTC Cuts at: 0 3244 3960 Size: 3244 716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2895 3960 Size: 2895 1065 XmaI C'CCGG_G Cuts at: 0 2615 3960 Size: 2615 1345 XmnI GAAnn'nnTTC Cuts at: 0 224 273 363 1733 2237 2366 3960 Size: 224 49 90 1370 504 129 1594 Fragments arranged by size: 1594 1370 504 224 129 90 49 ZraI GAC'GTC Cuts at: 0 820 3960 Size: 820 3140 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AhdI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BclI BfrBI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII KasI KpnI MmeI MslI MspA1I NarI NdeI NsiI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AflIII AgeI AleI AloI AscI AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsiEI BsiWI BsrBI BsrGI BssHII BssSI BstBI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII EagI FseI FspI FspAI HincII HpaI MfeI MluI MscI NaeI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI