Restriction map for pF1KSDA0642
 (Linear) (Six-base) MAPSORT of: KIAA0642.seq  Check: 5624  from: 1  to: 3960



KIAA0642.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1943    3960 
   Size:       1943    2017
 
AatII G_ACGT'C

Cuts at:      0     822    3960 
   Size:        822    3138
 
AccI GT'mk_AC

Cuts at:      0    1314    3960 
   Size:       1314    2646
 
Acc65I G'GTAC_C

Cuts at:      0     243    3052    3960 
   Size:        243    2809     908

  Fragments arranged by size: 

                2809     908     243
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     965    1196    1307    1370    2128    2224    2971 
   Size:        161     804     231     111      63     758      96     747

Cuts at:   2971    3032    3448    3960 
   Size:         61     416     512

  Fragments arranged by size: 

                 804     758     747     512     416     231     161     111
                  96      63      61
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1221    3960 
   Size:       1221    2739
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     478    2483    3960 
   Size:        478    2005    1477

  Fragments arranged by size: 

                2005    1477     478
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21    1371    1507    1935    2125    2224    3687    3960 
   Size:         21    1350     136     428     190      99    1463     273

  Fragments arranged by size: 

                1463    1350     428     273     190     136      99      21
            
 
ApaI G_GGCC'C

Cuts at:      0    2616    3960 
   Size:       2616    1344
 
ApaLI G'TGCA_C

Cuts at:      0    1577    3960 
   Size:       1577    2383
 
ApoI r'AATT_y

Cuts at:      0     196     606     649    1694    2363    3189    3330    3960 
   Size:        196     410      43    1045     669     826     141     630

  Fragments arranged by size: 

                1045     826     669     630     410     196     141      43
            
 
AvaI C'yCGr_G

Cuts at:      0    2615    3960 
   Size:       2615    1345
 
AvrII C'CTAG_G

Cuts at:      0    3325    3960 
   Size:       3325     635
 
BanI G'GyrC_C

Cuts at:      0     243    2957    3052    3960 
   Size:        243    2714      95     908

  Fragments arranged by size: 

                2714     908     243      95
 
BanII G_rGCy'C

Cuts at:      0      51     348    2616    3150    3205    3960 
   Size:         51     297    2268     534      55     755

  Fragments arranged by size: 

                2268     755     534     297      55      51
 
BbeI G_GCGC'C

Cuts at:      0    2961    3960 
   Size:       2961     999
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2000    2355    2532    3960 
   Size:       2000     355     177    1428

  Fragments arranged by size: 

                2000    1428     355     177
 
BclI T'GATC_A

Cuts at:      0    2076    3960 
   Size:       2076    1884
 
BfrBI ATG'CAT

Cuts at:      0     465    1650    3910    3960 
   Size:        465    1185    2260      50

  Fragments arranged by size: 

                2260    1185     465      50
 
BglI GCCn_nnn'nGGC

Cuts at:      0      39    3960 
   Size:         39    3921
 
Bme1580I G_kGCm'C

Cuts at:      0    1331    1581    2616    3960 
   Size:       1331     250    1035    1344

  Fragments arranged by size: 

                1344    1331    1035     250
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3878    3960 
   Size:       3878      82
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1083    3960 
   Size:       1083    2877
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     196     995    1826    2743    3091    3960 
   Size:         74     122     799     831     917     348     869

  Fragments arranged by size: 

                 917     869     831     799     348     122      74
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3658    3960 
   Size:       3658     302
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     594    1528    3960 
   Size:        594     934    2432

  Fragments arranged by size: 

                2432     934     594
 
BsaBI GATnn'nnATC

Cuts at:      0    1599    3960 
   Size:       1599    2361
 
BsaHI Gr'CG_yC

Cuts at:      0     819    2958    3960 
   Size:        819    2139    1002

  Fragments arranged by size: 

                2139    1002     819
 
BsaWI w'CCGG_w

Cuts at:      0    2780    3960 
   Size:       2780    1180
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      63      93     320     350    1383    1413    3204    3234 
   Size:         63      30     227      30    1033      30    1791      30

Cuts at:   3234    3960 
   Size:        726

  Fragments arranged by size: 

                1791    1033     726     227      63      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      71     335     338     341     461    1075    1306    1391 
   Size:         71     264       3       3     120     614     231      85

Cuts at:   1391    1850    2553    2611    2662    2686    3960 
   Size:        459     703      58      51      24    1274

  Fragments arranged by size: 

                1274     703     614     459     264     231     120      85
                  71      58      51      24       3       3
 
BseYI C'CCAG_C

Cuts at:      0     479     576    1330    2012    2633    3960 
   Size:        479      97     754     682     621    1327

  Fragments arranged by size: 

                1327     754     682     621     479      97
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3157    3856    3960 
   Size:       3157     699     104

  Fragments arranged by size: 

                3157     699     104
 
BsiHKAI G_wGCw'C

Cuts at:      0      51    1069    1479    1581    1619    3150    3960 
   Size:         51    1018     410     102      38    1531     810

  Fragments arranged by size: 

                1531    1018     810     410     102      51      38
 
BsmI GAATG_Cn'

Cuts at:      0     979    3162    3960 
   Size:        979    2183     798

  Fragments arranged by size: 

                2183     979     798
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3937    3960 
   Size:       3937      23
 
Bsp1286I G_dGCh'C

Cuts at:      0      51     348    1069    1331    1479    1581    1619    2616 
   Size:         51     297     721     262     148     102      38     997

Cuts at:   2616    3150    3205    3960 
   Size:        534      55     755

  Fragments arranged by size: 

                 997     755     721     534     297     262     148     102
                  55      51      38
 
BspEI T'CCGG_A

Cuts at:      0    2780    3960 
   Size:       2780    1180
 
BspHI T'CATG_A

Cuts at:      0    1811    3960 
   Size:       1811    2149
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1943    3179    3960 
   Size:       1943    1236     781

  Fragments arranged by size: 

                1943    1236     781
 
BsrDI GCAATG_nn'

Cuts at:      0      94    1664    3960 
   Size:         94    1570    2296

  Fragments arranged by size: 

                2296    1570      94
 
BsrFI r'CCGG_y

Cuts at:      0    2654    3389    3960 
   Size:       2654     735     571

  Fragments arranged by size: 

                2654     735     571
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1935    3687    3960 
   Size:       1935    1752     273

  Fragments arranged by size: 

                1935    1752     273
 
BstEII G'GTnAC_C

Cuts at:      0     865    3960 
   Size:        865    3095
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1801    3960 
   Size:       1801    2159
 
BstYI r'GATC_y

Cuts at:      0    1680    2193    3074    3879    3960 
   Size:       1680     513     881     805      81

  Fragments arranged by size: 

                1680     881     805     513      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1685    3960 
   Size:       1685    2275
 
BtsI GCAGTG_nn'

Cuts at:      0     894    1497    3314    3478    3960 
   Size:        894     603    1817     164     482

  Fragments arranged by size: 

                1817     894     603     482     164
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     770    3960 
   Size:        770    3190
 
EaeI y'GGCC_r

Cuts at:      0    1450    3960 
   Size:       1450    2510
 
EarI CTCTTCn'nnn_

Cuts at:      0    1016    1088    1283    1409    1765    2280    2309    2315 
   Size:       1016      72     195     126     356     515      29       6

Cuts at:   2315    2429    2483    2528    2538    2543    2588    2663    2704 
   Size:        114      54      45      10       5      45      75      41

Cuts at:   2704    2942    2990    3071    3080    3960 
   Size:        238      48      81       9     880

  Fragments arranged by size: 

                1016     880     515     356     238     195     126     114
                  81      75      72      54      48      45      45      41
                  29      10       9       6       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2577    3960 
   Size:       2577    1383
 
EcoICRI GAG'CTC

Cuts at:      0      49    3148    3960 
   Size:         49    3099     812

  Fragments arranged by size: 

                3099     812      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     161     196     965     995    1196    1307    1370 
   Size:         74      87      35     769      30     201     111      63

Cuts at:   1370    1826    2128    2224    2743    2971    3032    3091    3448 
   Size:        456     302      96     519     228      61      59     357

Cuts at:   3448    3960 
   Size:        512

  Fragments arranged by size: 

                 769     519     512     456     357     302     228     201
                 111      96      87      74      63      61      59      35
                  30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     214    3960 
   Size:        214    3746
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1702    2221    3322    3587    3960 
   Size:       1702     519    1101     265     373

  Fragments arranged by size: 

                1702    1101     519     373     265
 
EcoRI G'AATT_C

Cuts at:      0    3189    3330    3960 
   Size:       3189     141     630

  Fragments arranged by size: 

                3189     630     141
 
EcoRV GAT'ATC

Cuts at:      0    1809    3960 
   Size:       1809    2151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2431    2536    3960 
   Size:       2431     105    1424

  Fragments arranged by size: 

                2431    1424     105
 
HaeII r_GCGC'y

Cuts at:      0    2961    3645    3960 
   Size:       2961     684     315

  Fragments arranged by size: 

                2961     684     315
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1207    1239    1381    1413    2113    2145    2967    2999 
   Size:       1207      32     142      32     700      32     822      32

Cuts at:   2999    3887    3919    3960 
   Size:        888      32      41

  Fragments arranged by size: 

                1207     888     822     700     142      41      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1788    2827    3960 
   Size:       1788    1039    1133

  Fragments arranged by size: 

                1788    1133    1039
 
KasI G'GCGC_C

Cuts at:      0    2957    3960 
   Size:       2957    1003
 
KpnI G_GTAC'C

Cuts at:      0     247    3056    3960 
   Size:        247    2809     904

  Fragments arranged by size: 

                2809     904     247
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    1818    3191    3308    3929    3960 
   Size:        802    1016    1373     117     621      31

  Fragments arranged by size: 

                1373    1016     802     621     117      31
 
MslI CAynn'nnrTG

Cuts at:      0    1816    3960 
   Size:       1816    2144
 
MspA1I CmG'CkG

Cuts at:      0       7    2454    2733    2814    3687    3960 
   Size:          7    2447     279      81     873     273

  Fragments arranged by size: 

                2447     873     279     273      81       7
 
NarI GG'CG_CC

Cuts at:      0    2958    3960 
   Size:       2958    1002
 
NdeI CA'TA_TG

Cuts at:      0    1865    3960 
   Size:       1865    2095
 
NsiI A_TGCA'T

Cuts at:      0     467    1652    3912    3960 
   Size:        467    1185    2260      48

  Fragments arranged by size: 

                2260    1185     467      48
 
NspI r_CATG'y

Cuts at:      0     558    2876    3960 
   Size:        558    2318    1084

  Fragments arranged by size: 

                2318    1084     558
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2125    3960 
   Size:       2125    1835
 
PfoI T'CCnGG_A

Cuts at:      0     212    3960 
   Size:        212    3748
 
PpuMI rG'GwC_Cy

Cuts at:      0    1702    3322    3960 
   Size:       1702    1620     638

  Fragments arranged by size: 

                1702    1620     638
 
PspOMI G'GGCC_C

Cuts at:      0    2612    3960 
   Size:       2612    1348
 
PstI C_TGCA'G

Cuts at:      0     795    1940    3960 
   Size:        795    1145    2020

  Fragments arranged by size: 

                2020    1145     795
 
PvuII CAG'CTG

Cuts at:      0    2814    3687    3960 
   Size:       2814     873     273

  Fragments arranged by size: 

                2814     873     273
 
SacI G_AGCT'C

Cuts at:      0      51    3150    3960 
   Size:         51    3099     810

  Fragments arranged by size: 

                3099     810      51
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1016    2315    2942    2990    3960 
   Size:       1016    1299     627      48     970

  Fragments arranged by size: 

                1299    1016     970     627      48
 
SexAI A'CCwGG_T

Cuts at:      0    1200    3960 
   Size:       1200    2760
 
SfcI C'TryA_G

Cuts at:      0     791    1936    2165    3374    3960 
   Size:        791    1145     229    1209     586

  Fragments arranged by size: 

                1209    1145     791     586     229
 
SfoI GGC'GCC

Cuts at:      0    2959    3960 
   Size:       2959    1001
 
SmaI CCC'GGG

Cuts at:      0    2617    3960 
   Size:       2617    1343
 
SmlI C'TyrA_G

Cuts at:      0    3637    3960 
   Size:       3637     323
 
StyI C'CwwG_G

Cuts at:      0     594    3325    3614    3960 
   Size:        594    2731     289     346

  Fragments arranged by size: 

                2731     594     346     289
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3922    3960 
   Size:       3922      38
 
TatI w'GTAC_w

Cuts at:      0     557     999    3772    3960 
   Size:        557     442    2773     188

  Fragments arranged by size: 

                2773     557     442     188
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     320     323     352     355    3739    3771    3960 
   Size:        320       3      29       3    3384      32     189

  Fragments arranged by size: 

                3384     320     189      32      29       3       3
 
Tth111I GACn'n_nGTC

Cuts at:      0    3244    3960 
   Size:       3244     716
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2895    3960 
   Size:       2895    1065
 
XmaI C'CCGG_G

Cuts at:      0    2615    3960 
   Size:       2615    1345
 
XmnI GAAnn'nnTTC

Cuts at:      0     224     273     363    1733    2237    2366    3960 
   Size:        224      49      90    1370     504     129    1594

  Fragments arranged by size: 

                1594    1370     504     224     129      90      49
 
ZraI GAC'GTC

Cuts at:      0     820    3960 
   Size:        820    3140
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AhdI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI     BclI    BfrBI     BglI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtsI
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     KasI     KpnI
     MmeI     MslI   MspA1I     NarI     NdeI     NsiI     NspI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI    SexAI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII   AflIII     AgeI     AleI     AloI     AscI
     AseI    AsiSI     BaeI    BamHI    BbvCI     BcgI    BciVI    BglII
     BlpI    BmgBI     BmtI   Bpu10I    BsaAI    BsiEI    BsiWI    BsrBI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     FseI     FspI    FspAI   HincII
     HpaI     MfeI     MluI     MscI     NaeI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI
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