Restriction map for
pF1KA0641
(Linear) (Six-base) MAPSORT of: KIAA0641.seq Check: 1379 from: 1 to: 3813
KIAA0641.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 714 875 1362 1965 2367 2911 3813
Size: 714 161 487 603 402 544 902
Fragments arranged by size:
902 714 603 544 487 402 161
AatII G_ACGT'C
Cuts at: 0 1448 3404 3813
Size: 1448 1956 409
Fragments arranged by size:
1956 1448 409
AccI GT'mk_AC
Cuts at: 0 1442 3410 3813
Size: 1442 1968 403
Fragments arranged by size:
1968 1442 403
Acc65I G'GTAC_C
Cuts at: 0 1856 3813
Size: 1856 1957
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 250 2433 3800 3813
Size: 94 156 2183 1367 13
Fragments arranged by size:
2183 1367 156 94 13
AfeI AGC'GCT
Cuts at: 0 2492 3813
Size: 2492 1321
AflIII A'CryG_T
Cuts at: 0 1733 2309 3743 3813
Size: 1733 576 1434 70
Fragments arranged by size:
1733 1434 576 70
AhdI GACnn_n'nnGTC
Cuts at: 0 2415 3762 3813
Size: 2415 1347 51
Fragments arranged by size:
2415 1347 51
AleI CACnn'nnGTG
Cuts at: 0 46 3742 3813
Size: 46 3696 71
Fragments arranged by size:
3696 71 46
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 188 3813
Size: 188 3625
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2509 2541 3813
Size: 2509 32 1272
Fragments arranged by size:
2509 1272 32
AlwNI CAG_nnn'CTG
Cuts at: 0 201 255 729 1203 1763 1894 1950 2903
Size: 201 54 474 474 560 131 56 953
Cuts at: 2903 3813
Size: 910
Fragments arranged by size:
953 910 560 474 474 201 131 56
54
ApaI G_GGCC'C
Cuts at: 0 228 968 974 989 1538 2608 2615 2969
Size: 228 740 6 15 549 1070 7 354
Cuts at: 2969 3050 3097 3813
Size: 81 47 716
Fragments arranged by size:
1070 740 716 549 354 228 81 47
15 7 6
ApaLI G'TGCA_C
Cuts at: 0 439 862 3813
Size: 439 423 2951
Fragments arranged by size:
2951 439 423
AscI GG'CGCG_CC
Cuts at: 0 1220 3813
Size: 1220 2593
AvaI C'yCGr_G
Cuts at: 0 835 967 1227 1846 2242 2608 2614 2692
Size: 835 132 260 619 396 366 6 78
Cuts at: 2692 2752 2845 2910 3437 3445 3459 3813
Size: 60 93 65 527 8 14 354
Fragments arranged by size:
835 619 527 396 366 354 260 132
93 78 65 60 14 8 6
BanI G'GyrC_C
Cuts at: 0 564 895 1647 1856 1953 2253 2349 3100
Size: 564 331 752 209 97 300 96 751
Cuts at: 3100 3261 3270 3463 3787 3813
Size: 161 9 193 324 26
Fragments arranged by size:
752 751 564 331 324 300 209 193
161 97 96 26 9
BanII G_rGCy'C
Cuts at: 0 228 680 968 974 989 1013 1177 1538
Size: 228 452 288 6 15 24 164 361
Cuts at: 1538 1642 1883 2030 2112 2570 2608 2615 2751
Size: 104 241 147 82 458 38 7 136
Cuts at: 2751 2897 2939 2969 3050 3097 3114 3215 3446
Size: 146 42 30 81 47 17 101 231
Cuts at: 3446 3455 3502 3813
Size: 9 47 311
Fragments arranged by size:
458 452 361 311 288 241 231 228
164 147 146 136 104 101 82 81
47 47 42 38 30 24 17 15
9 7 6
BbeI G_GCGC'C
Cuts at: 0 899 1651 3265 3467 3813
Size: 899 752 1614 202 346
Fragments arranged by size:
1614 899 752 346 202
BbsI GAAGACnn'nnnn_
Cuts at: 0 2439 3813
Size: 2439 1374
BbvCI CC'TCA_GC
Cuts at: 0 1805 2048 2282 2421 2699 3813
Size: 1805 243 234 139 278 1114
Fragments arranged by size:
1805 1114 278 243 234 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 417 440 451 600 634 2179 2213
Size: 406 11 23 11 149 34 1545 34
Cuts at: 2213 3813
Size: 1600
Fragments arranged by size:
1600 1545 406 149 34 34 23 11
11
BglI GCCn_nnn'nGGC
Cuts at: 0 1162 1405 2044 2912 3813
Size: 1162 243 639 868 901
Fragments arranged by size:
1162 901 868 639 243
BlpI GC'TnA_GC
Cuts at: 0 1171 1246 3813
Size: 1171 75 2567
Fragments arranged by size:
2567 1171 75
Bme1580I G_kGCm'C
Cuts at: 0 144 228 443 694 866 968 974 989
Size: 144 84 215 251 172 102 6 15
Cuts at: 989 1538 1624 1721 2056 2139 2608 2615 2969
Size: 549 86 97 335 83 469 7 354
Cuts at: 2969 3050 3097 3275 3813
Size: 81 47 178 538
Fragments arranged by size:
549 538 469 354 335 251 215 178
172 144 102 97 86 84 83 81
47 15 7 6
BmgBI CAC'GTC
Cuts at: 0 48 1083 1625 2312 3722 3813
Size: 48 1035 542 687 1410 91
Fragments arranged by size:
1410 1035 687 542 91 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 319 1340 1584 1627 1811 2789 2979
Size: 255 64 1021 244 43 184 978 190
Cuts at: 2979 3813
Size: 834
Fragments arranged by size:
1021 978 834 255 244 190 184 64
43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 2307 2607 2730 3133 3813
Size: 141 2166 300 123 403 680
Fragments arranged by size:
2166 680 403 300 141 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 232 1060 1867 1883 2495 3813
Size: 223 9 828 807 16 612 1318
Fragments arranged by size:
1318 828 807 612 223 16 9
Bpu10I CC'TnA_GC
Cuts at: 0 1641 1805 2048 2282 2421 2699 3813
Size: 1641 164 243 234 139 278 1114
Fragments arranged by size:
1641 1114 278 243 234 164 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 304 742 2527 3307 3813
Size: 19 285 438 1785 780 506
Fragments arranged by size:
1785 780 506 438 285 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 1093 3340 3813
Size: 1093 2247 473
Fragments arranged by size:
2247 1093 473
BsaAI yAC'GTr
Cuts at: 0 3744 3813
Size: 3744 69
BsaHI Gr'CG_yC
Cuts at: 0 277 410 896 1445 1648 2144 3262 3401
Size: 277 133 486 549 203 496 1118 139
Cuts at: 3401 3464 3758 3813
Size: 63 294 55
Fragments arranged by size:
1118 549 496 486 294 277 203 139
133 63 55
BsaWI w'CCGG_w
Cuts at: 0 1239 2059 2931 3813
Size: 1239 820 872 882
Fragments arranged by size:
1239 882 872 820
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1711 1741 3669 3699 3813
Size: 1711 30 1928 30 114
Fragments arranged by size:
1928 1711 114 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 220 871 925 928 1309 1571 1699
Size: 22 198 651 54 3 381 262 128
Cuts at: 1699 2140 3181 3184 3187 3190 3223 3271 3813
Size: 441 1041 3 3 3 33 48 542
Fragments arranged by size:
1041 651 542 441 381 262 198 128
54 48 33 22 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 687 707 773 993 1266 1422 1709 2257
Size: 687 20 66 220 273 156 287 548
Cuts at: 2257 2763 2896 2946 2968 2974 3331 3511 3813
Size: 506 133 50 22 6 357 180 302
Fragments arranged by size:
687 548 506 357 302 287 273 220
180 156 133 66 50 22 20 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 202 232 400 3813
Size: 46 156 30 168 3413
Fragments arranged by size:
3413 168 156 46 30
BsiEI CG_ry'CG
Cuts at: 0 87 3163 3529 3650 3813
Size: 87 3076 366 121 163
Fragments arranged by size:
3076 366 163 121 87
BsiHKAI G_wGCw'C
Cuts at: 0 443 680 866 1013 1247 1642 2543 2779
Size: 443 237 186 147 234 395 901 236
Cuts at: 2779 3215 3446 3813
Size: 436 231 367
Fragments arranged by size:
901 443 436 395 367 237 236 234
231 186 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1791 3813
Size: 1791 2022
Bsp1286I G_dGCh'C
Cuts at: 0 144 228 443 680 694 866 968 974
Size: 144 84 215 237 14 172 102 6
Cuts at: 974 989 1013 1177 1247 1538 1624 1642 1721
Size: 15 24 164 70 291 86 18 79
Cuts at: 1721 1883 2030 2056 2112 2139 2543 2570 2608
Size: 162 147 26 56 27 404 27 38
Cuts at: 2608 2615 2751 2779 2897 2939 2969 3050 3097
Size: 7 136 28 118 42 30 81 47
Cuts at: 3097 3114 3215 3275 3446 3455 3502 3813
Size: 17 101 60 171 9 47 311
Fragments arranged by size:
404 311 291 237 215 172 171 164
162 147 144 136 118 102 101 86
84 81 79 70 60 56 47 47
42 38 30 28 27 27 26 24
18 17 15 14 9 7 6
BspEI T'CCGG_A
Cuts at: 0 2931 3813
Size: 2931 882
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 293 338 623 714 875 1362 1965
Size: 251 42 45 285 91 161 487 603
Cuts at: 1965 2367 2787 2911 3317 3813
Size: 402 420 124 406 496
Fragments arranged by size:
603 496 487 420 406 402 285 251
161 124 91 45 42
BsrBI CCG'CTC
Cuts at: 0 641 1500 1561 2363 3685 3813
Size: 641 859 61 802 1322 128
Fragments arranged by size:
1322 859 802 641 128 61
BsrFI r'CCGG_y
Cuts at: 0 53 1312 1333 3069 3158 3264 3524 3813
Size: 53 1259 21 1736 89 106 260 289
Fragments arranged by size:
1736 1259 289 260 106 89 53 21
BssHII G'CGCG_C
Cuts at: 0 1220 1250 3299 3301 3813
Size: 1220 30 2049 2 512
Fragments arranged by size:
2049 1220 512 30 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 870 1894 3813
Size: 870 1024 1919
Fragments arranged by size:
1919 1024 870
BstEII G'GTnAC_C
Cuts at: 0 1092 2008 3813
Size: 1092 916 1805
Fragments arranged by size:
1805 1092 916
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 1775 2208 3813
Size: 48 1727 433 1605
Fragments arranged by size:
1727 1605 433 48
Bsu36I CC'TnA_GG
Cuts at: 0 2292 2715 3490 3813
Size: 2292 423 775 323
Fragments arranged by size:
2292 775 423 323
BtgI C'CryG_G
Cuts at: 0 87 356 1143 1361 1409 1428 1458 1834
Size: 87 269 787 218 48 19 30 376
Cuts at: 1834 2039 3275 3632 3813
Size: 205 1236 357 181
Fragments arranged by size:
1236 787 376 357 269 218 205 181
87 48 30 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 409 3813
Size: 409 3404
BtsI GCAGTG_nn'
Cuts at: 0 144 516 2268 2863 3813
Size: 144 372 1752 595 950
Fragments arranged by size:
1752 950 595 372 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 3406 3813
Size: 3406 407
EaeI y'GGCC_r
Cuts at: 0 2 84 1163 1179 1314 1406 1583 2459
Size: 2 82 1079 16 135 92 177 876
Cuts at: 2459 3160 3526 3614 3647 3813
Size: 701 366 88 33 166
Fragments arranged by size:
1079 876 701 366 177 166 135 92
88 82 33 16 2
EagI C'GGCC_G
Cuts at: 0 84 3160 3526 3647 3813
Size: 84 3076 366 121 166
Fragments arranged by size:
3076 366 166 121 84
EarI CTCTTCn'nnn_
Cuts at: 0 3248 3563 3813
Size: 3248 315 250
Fragments arranged by size:
3248 315 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 364 1276 1564 3109 3813
Size: 364 912 288 1545 704
Fragments arranged by size:
1545 912 704 364 288
EcoICRI GAG'CTC
Cuts at: 0 678 1011 1640 3213 3444 3813
Size: 678 333 629 1573 231 369
Fragments arranged by size:
1573 678 629 369 333 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 223 232 250 1060 1867 1883 2433
Size: 94 129 9 18 810 807 16 550
Cuts at: 2433 2495 3800 3813
Size: 62 1305 13
Fragments arranged by size:
1305 810 807 550 129 94 62 18
16 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 205 1294 3813
Size: 115 90 1089 2519
Fragments arranged by size:
2519 1089 115 90
EcoO109I rG'GnC_Cy
Cuts at: 0 224 225 315 964 985 1494 1534 1535
Size: 224 1 90 649 21 509 40 1
Cuts at: 1535 1611 1779 1947 2384 2604 2605 2882 3046
Size: 76 168 168 437 220 1 277 164
Cuts at: 3046 3047 3093 3360 3775 3813
Size: 1 46 267 415 38
Fragments arranged by size:
649 509 437 415 277 267 224 220
168 168 164 90 76 46 40 38
21 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3642 3813
Size: 3642 171
FseI GG_CCGG'CC
Cuts at: 0 3073 3162 3813
Size: 3073 89 651
Fragments arranged by size:
3073 651 89
FspI TGC'GCA
Cuts at: 0 3313 3813
Size: 3313 500
HaeII r_GCGC'y
Cuts at: 0 21 899 930 936 1651 1728 2494 3265
Size: 21 878 31 6 715 77 766 771
Cuts at: 3265 3467 3518 3813
Size: 202 51 295
Fragments arranged by size:
878 771 766 715 295 202 77 51
31 21 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1363 1395 1771 1803 2401 2433 3748 3780
Size: 1363 32 376 32 598 32 1315 32
Cuts at: 3780 3813
Size: 33
Fragments arranged by size:
1363 1315 598 376 33 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1443 3813
Size: 1443 2370
HindIII A'AGCT_T
Cuts at: 0 1482 2032 3813
Size: 1482 550 1781
Fragments arranged by size:
1781 1482 550
KasI G'GCGC_C
Cuts at: 0 895 1647 3261 3463 3813
Size: 895 752 1614 202 350
Fragments arranged by size:
1614 895 752 350 202
KpnI G_GTAC'C
Cuts at: 0 1860 3813
Size: 1860 1953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1754 3813
Size: 773 981 2059
Fragments arranged by size:
2059 981 773
MscI TGG'CCA
Cuts at: 0 4 1408 3813
Size: 4 1404 2405
Fragments arranged by size:
2405 1404 4
MslI CAynn'nnrTG
Cuts at: 0 46 3742 3813
Size: 46 3696 71
Fragments arranged by size:
3696 71 46
MspA1I CmG'CkG
Cuts at: 0 558 777 783 800 843 1018 1035 1145
Size: 558 219 6 17 43 175 17 110
Cuts at: 1145 1363 1419 1460 1656 1662 1754 1763 2978
Size: 218 56 41 196 6 92 9 1215
Cuts at: 2978 3012 3218 3565 3606 3813
Size: 34 206 347 41 207
Fragments arranged by size:
1215 558 347 219 218 207 206 196
175 110 92 56 43 41 41 34
17 17 9 6 6
NaeI GCC'GGC
Cuts at: 0 55 1314 1335 3071 3160 3266 3813
Size: 55 1259 21 1736 89 106 547
Fragments arranged by size:
1736 1259 547 106 89 55 21
NarI GG'CG_CC
Cuts at: 0 896 1648 3262 3464 3813
Size: 896 752 1614 202 349
Fragments arranged by size:
1614 896 752 349 202
NcoI C'CATG_G
Cuts at: 0 356 1409 3632 3813
Size: 356 1053 2223 181
Fragments arranged by size:
2223 1053 356 181
NgoMIV G'CCGG_C
Cuts at: 0 53 1312 1333 3069 3158 3264 3813
Size: 53 1259 21 1736 89 106 549
Fragments arranged by size:
1736 1259 549 106 89 53 21
PasI CC'CwG_GG
Cuts at: 0 657 1451 1944 3813
Size: 657 794 493 1869
Fragments arranged by size:
1869 794 657 493
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 255 1950 3813
Size: 150 105 1695 1863
Fragments arranged by size:
1863 1695 150 105
PfoI T'CCnGG_A
Cuts at: 0 200 743 1981 3230 3813
Size: 200 543 1238 1249 583
Fragments arranged by size:
1249 1238 583 543 200
PmlI CAC'GTG
Cuts at: 0 3744 3813
Size: 3744 69
PpuMI rG'GwC_Cy
Cuts at: 0 315 1494 1611 1779 2384 2882 3360 3775
Size: 315 1179 117 168 605 498 478 415
Cuts at: 3775 3813
Size: 38
Fragments arranged by size:
1179 605 498 478 415 315 168 117
38
PshAI GACnn'nnGTC
Cuts at: 0 3060 3813
Size: 3060 753
PspOMI G'GGCC_C
Cuts at: 0 224 964 970 985 1534 2604 2611 2965
Size: 224 740 6 15 549 1070 7 354
Cuts at: 2965 3046 3093 3813
Size: 81 47 720
Fragments arranged by size:
1070 740 720 549 354 224 81 47
15 7 6
PspXI vC'TCGA_Gb
Cuts at: 0 2692 3813
Size: 2692 1121
PstI C_TGCA'G
Cuts at: 0 167 383 782 833 1199 2330 2372 2873
Size: 167 216 399 51 366 1131 42 501
Cuts at: 2873 3813
Size: 940
Fragments arranged by size:
1131 940 501 399 366 216 167 51
42
PvuII CAG'CTG
Cuts at: 0 558 777 783 2978 3813
Size: 558 219 6 2195 835
Fragments arranged by size:
2195 835 558 219 6
RsrII CG'GwC_CG
Cuts at: 0 741 795 3813
Size: 741 54 3018
Fragments arranged by size:
3018 741 54
SacI G_AGCT'C
Cuts at: 0 680 1013 1642 3215 3446 3813
Size: 680 333 629 1573 231 367
Fragments arranged by size:
1573 680 629 367 333 231
SacII CC_GC'GG
Cuts at: 0 1146 1364 1461 3813
Size: 1146 218 97 2352
Fragments arranged by size:
2352 1146 218 97
SalI G'TCGA_C
Cuts at: 0 1441 3813
Size: 1441 2372
SanDI GG'GwC_CC
Cuts at: 0 1494 1779 2384 3813
Size: 1494 285 605 1429
Fragments arranged by size:
1494 1429 605 285
SbfI CC_TGCA'GG
Cuts at: 0 1199 2330 3813
Size: 1199 1131 1483
Fragments arranged by size:
1483 1199 1131
ScaI AGT'ACT
Cuts at: 0 1279 3813
Size: 1279 2534
SfcI C'TryA_G
Cuts at: 0 163 219 379 778 829 1195 2326 2368
Size: 163 56 160 399 51 366 1131 42
Cuts at: 2368 2869 3222 3813
Size: 501 353 591
Fragments arranged by size:
1131 591 501 399 366 353 163 160
56 51 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1162 3813
Size: 1162 2651
SfoI GGC'GCC
Cuts at: 0 897 1649 3263 3465 3813
Size: 897 752 1614 202 348
Fragments arranged by size:
1614 897 752 348 202
SgrAI Cr'CCGG_yG
Cuts at: 0 3264 3813
Size: 3264 549
SmaI CCC'GGG
Cuts at: 0 969 1229 2610 2754 2847 3439 3461 3813
Size: 969 260 1381 144 93 592 22 352
Fragments arranged by size:
1381 969 592 352 260 144 93 22
SmlI C'TyrA_G
Cuts at: 0 34 319 757 2542 2692 3322 3813
Size: 34 285 438 1785 150 630 491
Fragments arranged by size:
1785 630 491 438 285 150 34
SrfI GCCC'GGGC
Cuts at: 0 969 3813
Size: 969 2844
StuI AGG'CCT
Cuts at: 0 1112 1156 2575 2811 3813
Size: 1112 44 1419 236 1002
Fragments arranged by size:
1419 1112 1002 236 44
StyI C'CwwG_G
Cuts at: 0 356 890 1409 2940 2951 3149 3467 3617
Size: 356 534 519 1531 11 198 318 150
Cuts at: 3617 3632 3813
Size: 15 181
Fragments arranged by size:
1531 534 519 356 318 198 181 150
15 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 552 1111 2271 2759 3813
Size: 552 559 1160 488 1054
Fragments arranged by size:
1160 1054 559 552 488
TatI w'GTAC_w
Cuts at: 0 524 1124 1277 3813
Size: 524 600 153 2536
Fragments arranged by size:
2536 600 524 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1711 1743 3669 3701 3813
Size: 1711 32 1926 32 112
Fragments arranged by size:
1926 1711 112 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 789 1783 3400 3813
Size: 789 994 1617 413
Fragments arranged by size:
1617 994 789 413
XhoI C'TCGA_G
Cuts at: 0 2692 3813
Size: 2692 1121
XmaI C'CCGG_G
Cuts at: 0 967 1227 2608 2752 2845 3437 3459 3813
Size: 967 260 1381 144 93 592 22 354
Fragments arranged by size:
1381 967 592 354 260 144 93 22
XmnI GAAnn'nnTTC
Cuts at: 0 106 3813
Size: 106 3707
ZraI GAC'GTC
Cuts at: 0 1446 3402 3813
Size: 1446 1956 411
Fragments arranged by size:
1956 1446 411
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstEII BstXI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PstI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI
SrfI StuI StyI TaqII TatI TstI Tth111I XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI ApoI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI
BsmI BspHI BsrDI BsrGI BssSI BstBI BstYI BstZ17I
ClaI CspCI DraI DraIII EcoRI EcoRV FspAI HpaI
MfeI MluI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PsiI PsrI PvuI SapI
SexAI SgfI SnaBI SpeI SphI SspI SwaI XbaI
XcmI