Restriction map for
pF1KSDA0640
(Linear) (Six-base) MAPSORT of: KIAA0640.seq Check: 4473 from: 1 to: 1755
KIAA0640.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 150 760 848 1114 1132 1330 1492
Size: 148 2 610 88 266 18 198 162
Cuts at: 1492 1755
Size: 263
Fragments arranged by size:
610 266 263 198 162 148 88 18
2
AflII C'TTAA_G
Cuts at: 0 427 1755
Size: 427 1328
AflIII A'CryG_T
Cuts at: 0 1271 1755
Size: 1271 484
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 1485 1755
Size: 1062 423 270
Fragments arranged by size:
1062 423 270
ApaLI G'TGCA_C
Cuts at: 0 117 1755
Size: 117 1638
ApoI r'AATT_y
Cuts at: 0 265 1755
Size: 265 1490
AseI AT'TA_AT
Cuts at: 0 394 599 1630 1755
Size: 394 205 1031 125
Fragments arranged by size:
1031 394 205 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 1274 1364 1755
Size: 1274 90 391
Fragments arranged by size:
1274 391 90
BciVI GTATCCnnnnn_n'
Cuts at: 0 398 1755
Size: 398 1357
BclI T'GATC_A
Cuts at: 0 1670 1755
Size: 1670 85
BfrBI ATG'CAT
Cuts at: 0 343 1755
Size: 343 1412
Bme1580I G_kGCm'C
Cuts at: 0 121 1755
Size: 121 1634
BmrI ACTGGGnnnn_n'
Cuts at: 0 139 1689 1755
Size: 139 1550 66
Fragments arranged by size:
1550 139 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 778 1755
Size: 778 977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1030 1084 1105 1528 1591 1755
Size: 1015 15 54 21 423 63 164
Fragments arranged by size:
1015 423 164 63 54 21 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1121 1537 1561 1755
Size: 79 1042 416 24 194
Fragments arranged by size:
1042 416 194 79 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 84 1755
Size: 84 1671
BsaWI w'CCGG_w
Cuts at: 0 977 1755
Size: 977 778
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1354 1384 1504 1534 1755
Size: 1354 30 120 30 221
Fragments arranged by size:
1354 221 120 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 467 1525 1755
Size: 31 436 1058 230
Fragments arranged by size:
1058 436 230 31
BseYI C'CCAG_C
Cuts at: 0 89 927 1755
Size: 89 838 828
Fragments arranged by size:
838 828 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1755
Size: 1124 631
BsiHKAI G_wGCw'C
Cuts at: 0 121 164 1755
Size: 121 43 1591
Fragments arranged by size:
1591 121 43
Bsp1286I G_dGCh'C
Cuts at: 0 121 164 1755
Size: 121 43 1591
Fragments arranged by size:
1591 121 43
BspEI T'CCGG_A
Cuts at: 0 977 1755
Size: 977 778
BspHI T'CATG_A
Cuts at: 0 138 1620 1755
Size: 138 1482 135
Fragments arranged by size:
1482 138 135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 431 1695 1755
Size: 431 1264 60
Fragments arranged by size:
1264 431 60
BsrFI r'CCGG_y
Cuts at: 0 575 1755
Size: 575 1180
BssSI C'ACGA_G
Cuts at: 0 1100 1755
Size: 1100 655
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1102 1755
Size: 1102 653
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1359 1755
Size: 1359 396
BstYI r'GATC_y
Cuts at: 0 735 1755
Size: 735 1020
BtgI C'CryG_G
Cuts at: 0 1496 1748 1755
Size: 1496 252 7
Fragments arranged by size:
1496 252 7
DraI TTT'AAA
Cuts at: 0 218 348 1755
Size: 218 130 1407
Fragments arranged by size:
1407 218 130
EarI CTCTTCn'nnn_
Cuts at: 0 152 404 1106 1322 1361 1709 1755
Size: 152 252 702 216 39 348 46
Fragments arranged by size:
702 348 252 216 152 46 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1453 1755
Size: 1453 302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 150 760 848 1015 1030 1084 1105
Size: 148 2 610 88 167 15 54 21
Cuts at: 1105 1114 1132 1330 1492 1528 1591 1755
Size: 9 18 198 162 36 63 164
Fragments arranged by size:
610 198 167 164 162 148 88 63
54 36 21 18 15 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1489 1755
Size: 1489 266
EcoO109I rG'GnC_Cy
Cuts at: 0 99 1482 1684 1755
Size: 99 1383 202 71
Fragments arranged by size:
1383 202 99 71
EcoRI G'AATT_C
Cuts at: 0 265 1755
Size: 265 1490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1162 1419 1755
Size: 1162 257 336
Fragments arranged by size:
1162 336 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 720 752 1354 1386 1755
Size: 720 32 602 32 369
Fragments arranged by size:
720 602 369 32 32
HindIII A'AGCT_T
Cuts at: 0 433 1183 1342 1755
Size: 433 750 159 413
Fragments arranged by size:
750 433 413 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 262 946 1340 1433 1755
Size: 218 44 684 394 93 322
Fragments arranged by size:
684 394 322 218 93 44
MspA1I CmG'CkG
Cuts at: 0 71 990 1506 1755
Size: 71 919 516 249
Fragments arranged by size:
919 516 249 71
NcoI C'CATG_G
Cuts at: 0 1496 1755
Size: 1496 259
NsiI A_TGCA'T
Cuts at: 0 345 1755
Size: 345 1410
NspI r_CATG'y
Cuts at: 0 210 1275 1755
Size: 210 1065 480
Fragments arranged by size:
1065 480 210
PciI A'CATG_T
Cuts at: 0 1271 1755
Size: 1271 484
PflMI CCAn_nnn'nTGG
Cuts at: 0 1598 1755
Size: 1598 157
PpuMI rG'GwC_Cy
Cuts at: 0 99 1684 1755
Size: 99 1585 71
Fragments arranged by size:
1585 99 71
PsiI TTA'TAA
Cuts at: 0 485 1755
Size: 485 1270
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1229 1261 1755
Size: 1229 32 494
Fragments arranged by size:
1229 494 32
PstI C_TGCA'G
Cuts at: 0 1289 1490 1692 1755
Size: 1289 201 202 63
Fragments arranged by size:
1289 202 201 63
PvuII CAG'CTG
Cuts at: 0 990 1506 1755
Size: 990 516 249
Fragments arranged by size:
990 516 249
SapI GCTCTTCn'nnn_
Cuts at: 0 152 1755
Size: 152 1603
SbfI CC_TGCA'GG
Cuts at: 0 1490 1755
Size: 1490 265
SexAI A'CCwGG_T
Cuts at: 0 1647 1755
Size: 1647 108
SfcI C'TryA_G
Cuts at: 0 1285 1486 1688 1755
Size: 1285 201 202 67
Fragments arranged by size:
1285 202 201 67
SmlI C'TyrA_G
Cuts at: 0 94 427 1136 1516 1540 1755
Size: 94 333 709 380 24 215
Fragments arranged by size:
709 380 333 215 94 24
StyI C'CwwG_G
Cuts at: 0 1496 1755
Size: 1496 259
TatI w'GTAC_w
Cuts at: 0 696 1755
Size: 696 1059
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 276 300 308 332 1755
Size: 276 24 8 24 1423
Fragments arranged by size:
1423 276 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 0 585 1755
Size: 585 1170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1503 1755
Size: 1503 252
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApaLI ApoI AseI BbsI
BciVI BclI BfrBI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrFI BssSI BstAPI BstXI BstYI
BtgI DraI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HindIII MmeI MspA1I NcoI NsiI NspI
PciI PflMI PpuMI PsiI PsrI PstI PvuII SapI
SbfI SexAI SfcI SmlI StyI TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BglI
BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EcoICRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII XbaI XhoI
XmaI XmnI ZraI