Restriction map for
pF1KSDA0637
(Linear) (Six-base) MAPSORT of: KIAA0637.seq Check: 7845 from: 1 to: 3513
KIAA0637.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2310 3513
Size: 2310 1203
AatII G_ACGT'C
Cuts at: 0 2663 3513
Size: 2663 850
AccI GT'mk_AC
Cuts at: 0 2027 3513
Size: 2027 1486
Acc65I G'GTAC_C
Cuts at: 0 1786 3513
Size: 1786 1727
AclI AA'CG_TT
Cuts at: 0 2033 3513
Size: 2033 1480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1390 2386 2466 2974 3263 3337 3374
Size: 716 674 996 80 508 289 74 37
Cuts at: 3374 3513
Size: 139
Fragments arranged by size:
996 716 674 508 289 139 80 74
37
AflII C'TTAA_G
Cuts at: 0 2158 3513
Size: 2158 1355
AflIII A'CryG_T
Cuts at: 0 713 989 2312 3513
Size: 713 276 1323 1201
Fragments arranged by size:
1323 1201 713 276
AgeI A'CCGG_T
Cuts at: 0 2582 3513
Size: 2582 931
AhdI GACnn_n'nnGTC
Cuts at: 0 1191 2664 3513
Size: 1191 1473 849
Fragments arranged by size:
1473 1191 849
AleI CACnn'nnGTG
Cuts at: 0 1882 3143 3290 3513
Size: 1882 1261 147 223
Fragments arranged by size:
1882 1261 223 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 322 354 3513
Size: 322 32 3159
Fragments arranged by size:
3159 322 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2544 3513
Size: 2544 969
ApaLI G'TGCA_C
Cuts at: 0 2578 3513
Size: 2578 935
ApoI r'AATT_y
Cuts at: 0 92 3108 3513
Size: 92 3016 405
Fragments arranged by size:
3016 405 92
AvaI C'yCGr_G
Cuts at: 0 210 383 1880 2466 2695 3513
Size: 210 173 1497 586 229 818
Fragments arranged by size:
1497 818 586 229 210 173
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 441 474 1770 1803 3513
Size: 441 33 1296 33 1710
Fragments arranged by size:
1710 1296 441 33 33
BamHI G'GATC_C
Cuts at: 0 3024 3513
Size: 3024 489
BanI G'GyrC_C
Cuts at: 0 1330 1477 1508 1786 1884 3513
Size: 1330 147 31 278 98 1629
Fragments arranged by size:
1629 1330 278 147 98 31
BanII G_rGCy'C
Cuts at: 0 340 1106 3513
Size: 340 766 2407
Fragments arranged by size:
2407 766 340
BbeI G_GCGC'C
Cuts at: 0 1512 3513
Size: 1512 2001
BbsI GAAGACnn'nnnn_
Cuts at: 0 1232 1789 2449 3000 3513
Size: 1232 557 660 551 513
Fragments arranged by size:
1232 660 557 551 513
BbvCI CC'TCA_GC
Cuts at: 0 595 2639 2860 3513
Size: 595 2044 221 653
Fragments arranged by size:
2044 653 595 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 778 812 3513
Size: 778 34 2701
Fragments arranged by size:
2701 778 34
BclI T'GATC_A
Cuts at: 0 2762 3513
Size: 2762 751
BfrBI ATG'CAT
Cuts at: 0 407 3513
Size: 407 3106
BglI GCCn_nnn'nGGC
Cuts at: 0 298 1538 1582 2271 3513
Size: 298 1240 44 689 1242
Fragments arranged by size:
1242 1240 689 298 44
BglII A'GATC_T
Cuts at: 0 2564 3513
Size: 2564 949
BlpI GC'TnA_GC
Cuts at: 0 1105 2548 3513
Size: 1105 1443 965
Fragments arranged by size:
1443 1105 965
Bme1580I G_kGCm'C
Cuts at: 0 970 1002 1036 1480 1897 2582 3513
Size: 970 32 34 444 417 685 931
Fragments arranged by size:
970 931 685 444 417 34 32
BmgBI CAC'GTC
Cuts at: 0 288 1230 2192 2313 3126 3513
Size: 288 942 962 121 813 387
Fragments arranged by size:
962 942 813 387 288 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 258 1951 2256 3513
Size: 258 1693 305 1257
Fragments arranged by size:
1693 1257 305 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 196 1125 3513
Size: 196 929 2388
Fragments arranged by size:
2388 929 196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 338 1229 1993 2085 2383 3286 3513
Size: 324 14 891 764 92 298 903 227
Fragments arranged by size:
903 891 764 324 298 227 92 14
Bpu10I CC'TnA_GC
Cuts at: 0 595 2639 2860 3513
Size: 595 2044 221 653
Fragments arranged by size:
2044 653 595 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1402 3513
Size: 474 928 2111
Fragments arranged by size:
2111 928 474
BsaI GGTCTCn'nnnn_
Cuts at: 0 1851 3513
Size: 1851 1662
BsaAI yAC'GTr
Cuts at: 0 480 3513
Size: 480 3033
BsaBI GATnn'nnATC
Cuts at: 0 2929 3513
Size: 2929 584
BsaHI Gr'CG_yC
Cuts at: 0 637 1273 1509 2660 3325 3513
Size: 637 636 236 1151 665 188
Fragments arranged by size:
1151 665 637 636 236 188
BsaWI w'CCGG_w
Cuts at: 0 1125 1872 2582 3513
Size: 1125 747 710 931
Fragments arranged by size:
1125 931 747 710
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 664 694 1991 2021 2356 2386
Size: 322 30 312 30 1297 30 335 30
Cuts at: 2386 3025 3055 3175 3205 3513
Size: 639 30 120 30 308
Fragments arranged by size:
1297 639 335 322 312 308 120 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 696 1149 1152 1857 2932 3070 3073
Size: 685 11 453 3 705 1075 138 3
Cuts at: 3073 3196 3283 3286 3513
Size: 123 87 3 227
Fragments arranged by size:
1075 705 685 453 227 138 123 87
11 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2494 2557 3513
Size: 2494 63 956
Fragments arranged by size:
2494 956 63
BsiEI CG_ry'CG
Cuts at: 0 500 660 2275 2587 3513
Size: 500 160 1615 312 926
Fragments arranged by size:
1615 926 500 312 160
BsiHKAI G_wGCw'C
Cuts at: 0 340 506 841 1106 2465 2582 2680 2845
Size: 340 166 335 265 1359 117 98 165
Cuts at: 2845 3513
Size: 668
Fragments arranged by size:
1359 668 340 335 265 166 165 117
98
BsmI GAATG_Cn'
Cuts at: 0 917 2348 3513
Size: 917 1431 1165
Fragments arranged by size:
1431 1165 917
BsmBI CGTCTCn'nnnn_
Cuts at: 0 767 1127 1846 2914 3513
Size: 767 360 719 1068 599
Fragments arranged by size:
1068 767 719 599 360
Bsp1286I G_dGCh'C
Cuts at: 0 340 506 841 970 1002 1036 1106 1480
Size: 340 166 335 129 32 34 70 374
Cuts at: 1480 1897 2465 2582 2680 2845 3513
Size: 417 568 117 98 165 668
Fragments arranged by size:
668 568 417 374 340 335 166 165
129 117 98 70 34 32
BspEI T'CCGG_A
Cuts at: 0 1872 3513
Size: 1872 1641
BspHI T'CATG_A
Cuts at: 0 470 1358 3104 3446 3513
Size: 470 888 1746 342 67
Fragments arranged by size:
1746 888 470 342 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 804 2310 2552 3513
Size: 804 1506 242 961
Fragments arranged by size:
1506 961 804 242
BsrBI CCG'CTC
Cuts at: 0 175 2574 2700 3300 3513
Size: 175 2399 126 600 213
Fragments arranged by size:
2399 600 213 175 126
BsrFI r'CCGG_y
Cuts at: 0 1134 1505 2395 2582 3513
Size: 1134 371 890 187 931
Fragments arranged by size:
1134 931 890 371 187
BssHII G'CGCG_C
Cuts at: 0 251 486 3513
Size: 251 235 3027
Fragments arranged by size:
3027 251 235
BssSI C'ACGA_G
Cuts at: 0 969 3513
Size: 969 2544
BstAPI GCAn_nnn'nTGC
Cuts at: 0 923 974 1484 2544 3513
Size: 923 51 510 1060 969
Fragments arranged by size:
1060 969 923 510 51
BstEII G'GTnAC_C
Cuts at: 0 589 2385 3513
Size: 589 1796 1128
Fragments arranged by size:
1796 1128 589
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3021 3513
Size: 3021 492
BstYI r'GATC_y
Cuts at: 0 644 2564 3024 3513
Size: 644 1920 460 489
Fragments arranged by size:
1920 644 489 460
BtgI C'CryG_G
Cuts at: 0 293 1406 1888 2876 2963 2998 3248 3513
Size: 293 1113 482 988 87 35 250 265
Fragments arranged by size:
1113 988 482 293 265 250 87 35
BtsI GCAGTG_nn'
Cuts at: 0 921 2292 2482 3513
Size: 921 1371 190 1031
Fragments arranged by size:
1371 1031 921 190
DraI TTT'AAA
Cuts at: 0 66 126 3513
Size: 66 60 3387
Fragments arranged by size:
3387 66 60
EaeI y'GGCC_r
Cuts at: 0 290 1746 2272 2609 3203 3331 3350 3432
Size: 290 1456 526 337 594 128 19 82
Cuts at: 3432 3513
Size: 81
Fragments arranged by size:
1456 594 526 337 290 128 82 81
19
EagI C'GGCC_G
Cuts at: 0 2272 3513
Size: 2272 1241
EarI CTCTTCn'nnn_
Cuts at: 0 68 811 1608 2917 3513
Size: 68 743 797 1309 596
Fragments arranged by size:
1309 797 743 596 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3079 3513
Size: 3079 434
EcoICRI GAG'CTC
Cuts at: 0 338 1104 3513
Size: 338 766 2409
Fragments arranged by size:
2409 766 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 338 716 1229 1390 1993 2085 2383
Size: 324 14 378 513 161 603 92 298
Cuts at: 2383 2386 2466 2974 3263 3286 3337 3374 3513
Size: 3 80 508 289 23 51 37 139
Fragments arranged by size:
603 513 508 378 324 298 289 161
139 92 80 51 37 23 14 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2653 2863 3138 3319 3513
Size: 2653 210 275 181 194
Fragments arranged by size:
2653 275 210 194 181
EcoO109I rG'GnC_Cy
Cuts at: 0 960 3513
Size: 960 2553
EcoRI G'AATT_C
Cuts at: 0 92 3108 3513
Size: 92 3016 405
Fragments arranged by size:
3016 405 92
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1619 3505 3513
Size: 1619 1886 8
Fragments arranged by size:
1886 1619 8
HaeII r_GCGC'y
Cuts at: 0 1512 1913 2305 3513
Size: 1512 401 392 1208
Fragments arranged by size:
1512 1208 401 392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 1177 1209 1482 1514 2650 2682
Size: 664 32 481 32 273 32 1136 32
Cuts at: 2682 2747 2779 3139 3171 3513
Size: 65 32 360 32 342
Fragments arranged by size:
1136 664 481 360 342 273 65 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1508 3513
Size: 1508 2005
KpnI G_GTAC'C
Cuts at: 0 1790 3513
Size: 1790 1723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 2288 3513
Size: 1355 933 1225
Fragments arranged by size:
1355 1225 933
MscI TGG'CCA
Cuts at: 0 292 3205 3434 3513
Size: 292 2913 229 79
Fragments arranged by size:
2913 292 229 79
MslI CAynn'nnrTG
Cuts at: 0 788 1882 2848 2875 3143 3290 3513
Size: 788 1094 966 27 268 147 223
Fragments arranged by size:
1094 966 788 268 223 147 27
MspA1I CmG'CkG
Cuts at: 0 161 200 865 1484 1793 1815 1890 2361
Size: 161 39 665 619 309 22 75 471
Cuts at: 2361 2529 2768 3000 3290 3513
Size: 168 239 232 290 223
Fragments arranged by size:
665 619 471 309 290 239 232 223
168 161 75 39 22
NarI GG'CG_CC
Cuts at: 0 1509 3513
Size: 1509 2004
NcoI C'CATG_G
Cuts at: 0 293 1406 2876 2963 3248 3513
Size: 293 1113 1470 87 285 265
Fragments arranged by size:
1470 1113 293 285 265 87
NsiI A_TGCA'T
Cuts at: 0 409 3513
Size: 409 3104
NspI r_CATG'y
Cuts at: 0 411 717 993 2694 3513
Size: 411 306 276 1701 819
Fragments arranged by size:
1701 819 411 306 276
PciI A'CATG_T
Cuts at: 0 713 989 3513
Size: 713 276 2524
Fragments arranged by size:
2524 713 276
PflMI CCAn_nnn'nTGG
Cuts at: 0 633 2532 3513
Size: 633 1899 981
Fragments arranged by size:
1899 981 633
PfoI T'CCnGG_A
Cuts at: 0 340 850 2101 2654 3513
Size: 340 510 1251 553 859
Fragments arranged by size:
1251 859 553 510 340
PmlI CAC'GTG
Cuts at: 0 480 3513
Size: 480 3033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 3086 3118 3513
Size: 322 32 2732 32 395
Fragments arranged by size:
2732 395 322 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 960 3513
Size: 960 2553
PshAI GACnn'nnGTC
Cuts at: 0 1371 3513
Size: 1371 2142
PspXI vC'TCGA_Gb
Cuts at: 0 2466 2695 3513
Size: 2466 229 818
Fragments arranged by size:
2466 818 229
PstI C_TGCA'G
Cuts at: 0 231 801 1593 2621 3513
Size: 231 570 792 1028 892
Fragments arranged by size:
1028 892 792 570 231
PvuI CG_AT'CG
Cuts at: 0 660 3513
Size: 660 2853
PvuII CAG'CTG
Cuts at: 0 200 1484 1793 2361 2768 3290 3513
Size: 200 1284 309 568 407 522 223
Fragments arranged by size:
1284 568 522 407 309 223 200
SacI G_AGCT'C
Cuts at: 0 340 1106 3513
Size: 340 766 2407
Fragments arranged by size:
2407 766 340
SacII CC_GC'GG
Cuts at: 0 1891 3001 3513
Size: 1891 1110 512
Fragments arranged by size:
1891 1110 512
SapI GCTCTTCn'nnn_
Cuts at: 0 811 2917 3513
Size: 811 2106 596
Fragments arranged by size:
2106 811 596
ScaI AGT'ACT
Cuts at: 0 220 2074 3513
Size: 220 1854 1439
Fragments arranged by size:
1854 1439 220
SfcI C'TryA_G
Cuts at: 0 227 683 797 1589 2334 2617 3513
Size: 227 456 114 792 745 283 896
Fragments arranged by size:
896 792 745 456 283 227 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 298 3513
Size: 298 3215
SfoI GGC'GCC
Cuts at: 0 1510 3513
Size: 1510 2003
SmaI CCC'GGG
Cuts at: 0 212 1882 3513
Size: 212 1670 1631
Fragments arranged by size:
1670 1631 212
SmlI C'TyrA_G
Cuts at: 0 453 1417 2158 2466 2695 3513
Size: 453 964 741 308 229 818
Fragments arranged by size:
964 818 741 453 308 229
StuI AGG'CCT
Cuts at: 0 1267 3040 3513
Size: 1267 1773 473
Fragments arranged by size:
1773 1267 473
StyI C'CwwG_G
Cuts at: 0 293 1406 2591 2876 2963 3206 3248 3429
Size: 293 1113 1185 285 87 243 42 181
Cuts at: 3429 3513
Size: 84
Fragments arranged by size:
1185 1113 293 285 243 181 87 84
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 627 1537 1970 2373 3426 3513
Size: 627 910 433 403 1053 87
Fragments arranged by size:
1053 910 627 433 403 87
TatI w'GTAC_w
Cuts at: 0 218 266 410 1052 2072 3071 3513
Size: 218 48 144 642 1020 999 442
Fragments arranged by size:
1020 999 642 442 218 144 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1452 1484 1991 2023 2236 2268 2356 2388
Size: 1452 32 507 32 213 32 88 32
Cuts at: 2388 3175 3207 3513
Size: 787 32 306
Fragments arranged by size:
1452 787 507 306 213 88 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2191 3447 3513
Size: 2191 1256 66
Fragments arranged by size:
2191 1256 66
XhoI C'TCGA_G
Cuts at: 0 2466 2695 3513
Size: 2466 229 818
Fragments arranged by size:
2466 818 229
XmaI C'CCGG_G
Cuts at: 0 210 1880 3513
Size: 210 1670 1633
Fragments arranged by size:
1670 1633 210
XmnI GAAnn'nnTTC
Cuts at: 0 424 877 934 1540 3513
Size: 424 453 57 606 1973
Fragments arranged by size:
1973 606 453 424 57
ZraI GAC'GTC
Cuts at: 0 2661 3513
Size: 2661 852
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI DraI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NarI NcoI NsiI NspI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspXI PstI PvuI PvuII
SacI SacII SapI ScaI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AlfI ApaI AscI AseI AsiSI AvrII BciVI
BmtI BseYI BsiWI BsrDI BsrGI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EcoRV FseI FspI
FspAI HincII HindIII HpaI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PsiI
PspOMI PsrI RsrII SalI SanDI SbfI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI