Restriction map for
pF1KA0628
(Linear) (Six-base) MAPSORT of: KIAA0628.seq Check: 883 from: 1 to: 1608
KIAA0628.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 228 1608
Size: 228 1380
AatII G_ACGT'C
Cuts at: 0 103 1078 1608
Size: 103 975 530
Fragments arranged by size:
975 530 103
AclI AA'CG_TT
Cuts at: 0 1502 1608
Size: 1502 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 322 714 1050 1466 1608
Size: 80 242 392 336 416 142
Fragments arranged by size:
416 392 336 242 142 80
AfeI AGC'GCT
Cuts at: 0 1063 1608
Size: 1063 545
AflIII A'CryG_T
Cuts at: 0 452 1608
Size: 452 1156
AhdI GACnn_n'nnGTC
Cuts at: 0 535 1608
Size: 535 1073
AleI CACnn'nnGTG
Cuts at: 0 859 1608
Size: 859 749
AlwNI CAG_nnn'CTG
Cuts at: 0 201 230 1608
Size: 201 29 1378
Fragments arranged by size:
1378 201 29
ApaLI G'TGCA_C
Cuts at: 0 288 1608
Size: 288 1320
ApoI r'AATT_y
Cuts at: 0 512 932 1100 1436 1608
Size: 512 420 168 336 172
Fragments arranged by size:
512 420 336 172 168
AvaI C'yCGr_G
Cuts at: 0 136 825 1608
Size: 136 689 783
Fragments arranged by size:
783 689 136
BamHI G'GATC_C
Cuts at: 0 1016 1608
Size: 1016 592
BanI G'GyrC_C
Cuts at: 0 671 1608
Size: 671 937
BanII G_rGCy'C
Cuts at: 0 128 161 176 350 490 742 826 1330
Size: 128 33 15 174 140 252 84 504
Cuts at: 1330 1608
Size: 278
Fragments arranged by size:
504 278 252 174 140 128 84 33
15
Bme1580I G_kGCm'C
Cuts at: 0 292 1608
Size: 292 1316
BmrI ACTGGGnnnn_n'
Cuts at: 0 1034 1608
Size: 1034 574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 547 1135 1554 1608
Size: 147 400 588 419 54
Fragments arranged by size:
588 419 400 147 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1130 1214 1222 1382 1608
Size: 542 588 84 8 160 226
Fragments arranged by size:
588 542 226 160 84 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 1563 1608
Size: 266 1297 45
Fragments arranged by size:
1297 266 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 583 690 942 1608
Size: 583 107 252 666
Fragments arranged by size:
666 583 252 107
BsaAI yAC'GTr
Cuts at: 0 455 1041 1608
Size: 455 586 567
Fragments arranged by size:
586 567 455
BsaHI Gr'CG_yC
Cuts at: 0 100 1075 1608
Size: 100 975 533
Fragments arranged by size:
975 533 100
BsaWI w'CCGG_w
Cuts at: 0 940 1608
Size: 940 668
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1187 1217 1608
Size: 1187 30 391
Fragments arranged by size:
1187 391 30
BseYI C'CCAG_C
Cuts at: 0 436 1088 1608
Size: 436 652 520
Fragments arranged by size:
652 520 436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 1401 1608
Size: 1327 74 207
Fragments arranged by size:
1327 207 74
BsiHKAI G_wGCw'C
Cuts at: 0 128 176 292 350 490 742 826 1262
Size: 128 48 116 58 140 252 84 436
Cuts at: 1262 1330 1608
Size: 68 278
Fragments arranged by size:
436 278 252 140 128 116 84 68
58 48
BsmI GAATG_Cn'
Cuts at: 0 712 880 895 964 1608
Size: 712 168 15 69 644
Fragments arranged by size:
712 644 168 69 15
Bsp1286I G_dGCh'C
Cuts at: 0 128 161 176 292 350 490 742 826
Size: 128 33 15 116 58 140 252 84
Cuts at: 826 1262 1330 1608
Size: 436 68 278
Fragments arranged by size:
436 278 252 140 128 116 84 68
58 33 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 1608
Size: 228 1380
BsrDI GCAATG_nn'
Cuts at: 0 801 969 1053 1608
Size: 801 168 84 555
Fragments arranged by size:
801 555 168 84
BssSI C'ACGA_G
Cuts at: 0 154 1608
Size: 154 1454
BstBI TT'CG_AA
Cuts at: 0 1292 1608
Size: 1292 316
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1563 1608
Size: 1563 45
BstYI r'GATC_y
Cuts at: 0 260 848 1016 1268 1608
Size: 260 588 168 252 340
Fragments arranged by size:
588 340 260 252 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 889 1608
Size: 889 719
BtsI GCAGTG_nn'
Cuts at: 0 637 1608
Size: 637 971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 699 734 783 818 867 902 951 986
Size: 699 35 49 35 49 35 49 35
Cuts at: 986 1035 1070 1608
Size: 49 35 538
Fragments arranged by size:
699 538 49 49 49 49 35 35
35 35 35
EaeI y'GGCC_r
Cuts at: 0 480 1608
Size: 480 1128
EarI CTCTTCn'nnn_
Cuts at: 0 1442 1608
Size: 1442 166
EcoICRI GAG'CTC
Cuts at: 0 126 174 348 488 740 824 1328 1608
Size: 126 48 174 140 252 84 504 280
Fragments arranged by size:
504 280 252 174 140 126 84 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 322 542 714 1050 1130 1214 1222
Size: 80 242 220 172 336 80 84 8
Cuts at: 1222 1382 1466 1608
Size: 160 84 142
Fragments arranged by size:
336 242 220 172 160 142 84 84
80 80 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 816 1608
Size: 816 792
EcoO109I rG'GnC_Cy
Cuts at: 0 782 1286 1551 1608
Size: 782 504 265 57
Fragments arranged by size:
782 504 265 57
EcoRI G'AATT_C
Cuts at: 0 512 932 1100 1608
Size: 512 420 168 508
Fragments arranged by size:
512 508 420 168
EcoRV GAT'ATC
Cuts at: 0 378 1608
Size: 378 1230
FspI TGC'GCA
Cuts at: 0 629 1608
Size: 629 979
HaeII r_GCGC'y
Cuts at: 0 1065 1608
Size: 1065 543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 70 102 521 553 1474 1506 1608
Size: 70 32 419 32 921 32 102
Fragments arranged by size:
921 419 102 70 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1145 1608
Size: 1145 463
MscI TGG'CCA
Cuts at: 0 482 1608
Size: 482 1126
MslI CAynn'nnrTG
Cuts at: 0 118 859 1565 1608
Size: 118 741 706 43
Fragments arranged by size:
741 706 118 43
NspI r_CATG'y
Cuts at: 0 436 1608
Size: 436 1172
PmlI CAC'GTG
Cuts at: 0 455 1608
Size: 455 1153
PspXI vC'TCGA_Gb
Cuts at: 0 825 1608
Size: 825 783
SacI G_AGCT'C
Cuts at: 0 128 176 350 490 742 826 1330 1608
Size: 128 48 174 140 252 84 504 278
Fragments arranged by size:
504 278 252 174 140 128 84 48
SfcI C'TryA_G
Cuts at: 0 1577 1608
Size: 1577 31
SmlI C'TyrA_G
Cuts at: 0 281 825 1542 1608
Size: 281 544 717 66
Fragments arranged by size:
717 544 281 66
SnaBI TAC'GTA
Cuts at: 0 1041 1608
Size: 1041 567
SphI G_CATG'C
Cuts at: 0 436 1608
Size: 436 1172
StuI AGG'CCT
Cuts at: 0 1035 1608
Size: 1035 573
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1102 1555 1608
Size: 1102 453 53
Fragments arranged by size:
1102 453 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1187 1219 1608
Size: 1187 32 389
Fragments arranged by size:
1187 389 32
Tth111I GACn'n_nGTC
Cuts at: 0 99 1608
Size: 99 1509
XhoI C'TCGA_G
Cuts at: 0 825 1608
Size: 825 783
XmnI GAAnn'nnTTC
Cuts at: 0 559 727 811 895 1063 1147 1315 1608
Size: 559 168 84 84 168 84 168 293
Fragments arranged by size:
559 293 168 168 168 84 84 84
ZraI GAC'GTC
Cuts at: 0 101 1076 1608
Size: 101 975 532
Fragments arranged by size:
975 532 101
Enzymes that do cut:
AarI AatII AclI AcuI AfeI AflIII AhdI AleI
AlwNI ApaLI ApoI AvaI BamHI BanI BanII Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BssSI BstBI BstXI BstYI BtgZI BtsI CspCI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HindIII MscI MslI NspI PmlI PspXI
SacI SfcI SmlI SnaBI SphI StuI TaqII TstI
Tth111I XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AflII AgeI AlfI AloI ApaI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
Bpu10I BsaBI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I
BtgI ClaI DraI DraIII DrdI EagI EciI FalI
FseI FspAI HincII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI
SrfI SspI StyI SwaI TatI XbaI XcmI XmaI