Restriction map for
pF1KSDA0626
(Linear) (Six-base) MAPSORT of: KIAA0626.seq Check: 576 from: 1 to: 1227
KIAA0626.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1210 1227
Size: 1210 17
AccI GT'mk_AC
Cuts at: 0 452 857 1227
Size: 452 405 370
Fragments arranged by size:
452 405 370
Acc65I G'GTAC_C
Cuts at: 0 1131 1227
Size: 1131 96
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1130 1227
Size: 898 232 97
Fragments arranged by size:
898 232 97
AflIII A'CryG_T
Cuts at: 0 368 370 1227
Size: 368 2 857
Fragments arranged by size:
857 368 2
AleI CACnn'nnGTG
Cuts at: 0 118 373 1227
Size: 118 255 854
Fragments arranged by size:
854 255 118
ApoI r'AATT_y
Cuts at: 0 804 1227
Size: 804 423
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 326 359 1227
Size: 326 33 868
Fragments arranged by size:
868 326 33
BanI G'GyrC_C
Cuts at: 0 394 1131 1227
Size: 394 737 96
Fragments arranged by size:
737 394 96
BanII G_rGCy'C
Cuts at: 0 133 1227
Size: 133 1094
BbsI GAAGACnn'nnnn_
Cuts at: 0 547 1227
Size: 547 680
BbvCI CC'TCA_GC
Cuts at: 0 935 1227
Size: 935 292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 691 725 877 911 1227
Size: 691 34 152 34 316
Fragments arranged by size:
691 316 152 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 75 354 1227
Size: 75 279 873
Fragments arranged by size:
873 279 75
Bme1580I G_kGCm'C
Cuts at: 0 50 140 1227
Size: 50 90 1087
Fragments arranged by size:
1087 90 50
BmgBI CAC'GTC
Cuts at: 0 1091 1227
Size: 1091 136
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 1227
Size: 663 564
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 740 836 1227
Size: 740 96 391
Fragments arranged by size:
740 391 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 806 808 1227
Size: 458 348 2 419
Fragments arranged by size:
458 419 348 2
Bpu10I CC'TnA_GC
Cuts at: 0 935 1227
Size: 935 292
BsaAI yAC'GTr
Cuts at: 0 371 1227
Size: 371 856
BsaHI Gr'CG_yC
Cuts at: 0 911 1227
Size: 911 316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 305 308 778 805 1045 1227
Size: 305 3 470 27 240 182
Fragments arranged by size:
470 305 240 182 27 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 907 1227
Size: 602 305 320
Fragments arranged by size:
602 320 305
Bsp1286I G_dGCh'C
Cuts at: 0 50 133 140 1227
Size: 50 83 7 1087
Fragments arranged by size:
1087 83 50 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1210 1227
Size: 1210 17
BsrDI GCAATG_nn'
Cuts at: 0 146 1227
Size: 146 1081
BssSI C'ACGA_G
Cuts at: 0 925 1227
Size: 925 302
BstEII G'GTnAC_C
Cuts at: 0 411 1227
Size: 411 816
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1003 1227
Size: 492 511 224
Fragments arranged by size:
511 492 224
BtgI C'CryG_G
Cuts at: 0 680 1227
Size: 680 547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 902 1227
Size: 902 325
BtsI GCAGTG_nn'
Cuts at: 0 1187 1227
Size: 1187 40
ClaI AT'CG_AT
Cuts at: 0 6 1227
Size: 6 1221
DraI TTT'AAA
Cuts at: 0 107 1227
Size: 107 1120
DrdI GACnn_nn'nnGTC
Cuts at: 0 448 1041 1227
Size: 448 593 186
Fragments arranged by size:
593 448 186
EarI CTCTTCn'nnn_
Cuts at: 0 7 266 698 1106 1227
Size: 7 259 432 408 121
Fragments arranged by size:
432 408 259 121 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 627 1063 1227
Size: 627 436 164
Fragments arranged by size:
627 436 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 806 808 898 1130 1227
Size: 458 348 2 90 232 97
Fragments arranged by size:
458 348 232 97 90 2
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1227
Size: 363 864
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 901 933 1075 1090 1107 1122
Size: 516 32 353 32 142 15 17 15
Cuts at: 1122 1227
Size: 105
Fragments arranged by size:
516 353 142 105 32 32 17 15
15
KpnI G_GTAC'C
Cuts at: 0 1135 1227
Size: 1135 92
MslI CAynn'nnrTG
Cuts at: 0 118 373 463 490 952 989 1227
Size: 118 255 90 27 462 37 238
Fragments arranged by size:
462 255 238 118 90 37 27
MspA1I CmG'CkG
Cuts at: 0 320 901 1227
Size: 320 581 326
Fragments arranged by size:
581 326 320
NcoI C'CATG_G
Cuts at: 0 680 1227
Size: 680 547
NspI r_CATG'y
Cuts at: 0 988 1227
Size: 988 239
PmlI CAC'GTG
Cuts at: 0 371 1227
Size: 371 856
PstI C_TGCA'G
Cuts at: 0 596 751 1071 1227
Size: 596 155 320 156
Fragments arranged by size:
596 320 156 155
SapI GCTCTTCn'nnn_
Cuts at: 0 7 698 1106 1227
Size: 7 691 408 121
Fragments arranged by size:
691 408 121 7
SfcI C'TryA_G
Cuts at: 0 201 592 747 1067 1227
Size: 201 391 155 320 160
Fragments arranged by size:
391 320 201 160 155
SphI G_CATG'C
Cuts at: 0 988 1227
Size: 988 239
StyI C'CwwG_G
Cuts at: 0 338 680 1227
Size: 338 342 547
Fragments arranged by size:
547 342 338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 371 436 589 682 1227
Size: 371 65 153 93 545
Fragments arranged by size:
545 371 153 93 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 564 596 955 987 1227
Size: 564 32 359 32 240
Fragments arranged by size:
564 359 240 32 32
XbaI T'CTAG_A
Cuts at: 0 648 1227
Size: 648 579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 233 1227
Size: 233 994
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI ApoI BaeI
BanI BanII BbsI BbvCI BcgI BciVI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaAI BsaHI BseRI BsgI
Bsp1286I BspMI BsrDI BssSI BstEII BstXI BtgI BtgZI
BtsI ClaI DraI DrdI EarI EciI Eco57MI FalI
Hin4I KpnI MslI MspA1I NcoI NspI PmlI PstI
SapI SfcI SphI StyI TaqII TstI XbaI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BclI BfrBI BglI BglII BlpI BmtI
BpuEI BsaI BsaBI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstYI BstZ17I Bsu36I CspCI DraIII
EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I
XhoI XmaI XmnI ZraI