Restriction map for
pF1KA0625
(Linear) (Six-base) MAPSORT of: KIAA0625.seq Check: 7065 from: 1 to: 8031
KIAA0625.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AatII G_ACGT'C
Cuts at: 0 1891 8031
Size: 1891 6140
AccI GT'mk_AC
Cuts at: 0 269 1067 5937 7112 8031
Size: 269 798 4870 1175 919
Fragments arranged by size:
4870 1175 919 798 269
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1334 2123 2699 3281 3404 3530 4027
Size: 1162 172 789 576 582 123 126 497
Cuts at: 4027 4364 4529 4727 4835 6507 7591 7645 8031
Size: 337 165 198 108 1672 1084 54 386
Fragments arranged by size:
1672 1162 1084 789 582 576 497 386
337 198 172 165 126 123 108 54
AfeI AGC'GCT
Cuts at: 0 58 8031
Size: 58 7973
AflII C'TTAA_G
Cuts at: 0 1261 5790 8031
Size: 1261 4529 2241
Fragments arranged by size:
4529 2241 1261
AflIII A'CryG_T
Cuts at: 0 7 381 680 1142 2230 2376 7162 7849
Size: 7 374 299 462 1088 146 4786 687
Cuts at: 7849 8031
Size: 182
Fragments arranged by size:
4786 1088 687 462 374 299 182 146
7
AleI CACnn'nnGTG
Cuts at: 0 1813 8031
Size: 1813 6218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6392 7787 8031
Size: 6392 1395 244
Fragments arranged by size:
6392 1395 244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7069 7101 8031
Size: 7069 32 930
Fragments arranged by size:
7069 930 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1345 3055 3999 4171 4297 7749 8031
Size: 1345 1710 944 172 126 3452 282
Fragments arranged by size:
3452 1710 1345 944 282 172 126
ApaI G_GGCC'C
Cuts at: 0 5162 8031
Size: 5162 2869
ApaLI G'TGCA_C
Cuts at: 0 6017 8031
Size: 6017 2014
ApoI r'AATT_y
Cuts at: 0 85 1366 1704 1786 2439 2662 3451 3817
Size: 85 1281 338 82 653 223 789 366
Cuts at: 3817 4146 4959 5310 5335 5527 6067 6123 6253
Size: 329 813 351 25 192 540 56 130
Cuts at: 6253 8031
Size: 1778
Fragments arranged by size:
1778 1281 813 789 653 540 366 351
338 329 223 192 130 85 82 56
25
AseI AT'TA_AT
Cuts at: 0 3982 6155 8031
Size: 3982 2173 1876
Fragments arranged by size:
3982 2173 1876
AvaI C'yCGr_G
Cuts at: 0 7680 7837 8031
Size: 7680 157 194
Fragments arranged by size:
7680 194 157
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2474 2507 8031
Size: 2474 33 5524
Fragments arranged by size:
5524 2474 33
BamHI G'GATC_C
Cuts at: 0 849 927 7668 8031
Size: 849 78 6741 363
Fragments arranged by size:
6741 849 363 78
BanI G'GyrC_C
Cuts at: 0 7333 8031
Size: 7333 698
BanII G_rGCy'C
Cuts at: 0 778 5162 6297 7687 7886 8031
Size: 778 4384 1135 1390 199 145
Fragments arranged by size:
4384 1390 1135 778 199 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 1891 3659 4095 4391 7060 8031
Size: 110 1781 1768 436 296 2669 971
Fragments arranged by size:
2669 1781 1768 971 436 296 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1044 1078 6281 6315 8031
Size: 1044 34 5203 34 1716
Fragments arranged by size:
5203 1716 1044 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4883 8031
Size: 4883 3148
BclI T'GATC_A
Cuts at: 0 1970 3933 6734 8031
Size: 1970 1963 2801 1297
Fragments arranged by size:
2801 1970 1963 1297
BfrBI ATG'CAT
Cuts at: 0 1905 4247 6240 6780 7126 8031
Size: 1905 2342 1993 540 346 905
Fragments arranged by size:
2342 1993 1905 905 540 346
BglII A'GATC_T
Cuts at: 0 2150 2742 3573 5493 8031
Size: 2150 592 831 1920 2538
Fragments arranged by size:
2538 2150 1920 831 592
Bme1580I G_kGCm'C
Cuts at: 0 5162 6021 8031
Size: 5162 859 2010
Fragments arranged by size:
5162 2010 859
BmgBI CAC'GTC
Cuts at: 0 4417 5540 8031
Size: 4417 1123 2491
Fragments arranged by size:
4417 2491 1123
BmrI ACTGGGnnnn_n'
Cuts at: 0 314 5370 6145 8031
Size: 314 5056 775 1886
Fragments arranged by size:
5056 1886 775 314
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4473 8031
Size: 4473 3558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4274 4443 5042 7718 7808 8011 8031
Size: 4274 169 599 2676 90 203 20
Fragments arranged by size:
4274 2676 599 203 169 90 20
Bpu10I CC'TnA_GC
Cuts at: 0 3833 6623 7750 8031
Size: 3833 2790 1127 281
Fragments arranged by size:
3833 2790 1127 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 2891 3109 3142 5172 5573 6508 7378
Size: 763 2128 218 33 2030 401 935 870
Cuts at: 7378 8031
Size: 653
Fragments arranged by size:
2128 2030 935 870 763 653 401 218
33
BsaI GGTCTCn'nnnn_
Cuts at: 0 2873 4292 4770 7939 8031
Size: 2873 1419 478 3169 92
Fragments arranged by size:
3169 2873 1419 478 92
BsaAI yAC'GTr
Cuts at: 0 3631 3931 7163 7850 8031
Size: 3631 300 3232 687 181
Fragments arranged by size:
3631 3232 687 300 181
BsaBI GATnn'nnATC
Cuts at: 0 455 6864 8031
Size: 455 6409 1167
Fragments arranged by size:
6409 1167 455
BsaHI Gr'CG_yC
Cuts at: 0 1888 6405 8031
Size: 1888 4517 1626
Fragments arranged by size:
4517 1888 1626
BsaWI w'CCGG_w
Cuts at: 0 79 1023 8031
Size: 79 944 7008
Fragments arranged by size:
7008 944 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3603 3633 5480 5510 5962 5992 6561 6591
Size: 3603 30 1847 30 452 30 569 30
Cuts at: 6591 7410 7440 8031
Size: 819 30 591
Fragments arranged by size:
3603 1847 819 591 569 452 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2788 3980 4745 5917 7894 8031
Size: 2788 1192 765 1172 1977 137
Fragments arranged by size:
2788 1977 1192 1172 765 137
BseYI C'CCAG_C
Cuts at: 0 1445 2053 3899 7471 7688 8031
Size: 1445 608 1846 3572 217 343
Fragments arranged by size:
3572 1846 1445 608 343 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1627 6441 8031
Size: 1627 4814 1590
Fragments arranged by size:
4814 1627 1590
BsiHKAI G_wGCw'C
Cuts at: 0 7 6021 6297 6469 7886 8031
Size: 7 6014 276 172 1417 145
Fragments arranged by size:
6014 1417 276 172 145 7
BsmI GAATG_Cn'
Cuts at: 0 1569 5964 7126 8031
Size: 1569 4395 1162 905
Fragments arranged by size:
4395 1569 1162 905
BsmBI CGTCTCn'nnnn_
Cuts at: 0 208 4871 8031
Size: 208 4663 3160
Fragments arranged by size:
4663 3160 208
Bsp1286I G_dGCh'C
Cuts at: 0 7 778 5162 6021 6297 6469 7687 7886
Size: 7 771 4384 859 276 172 1218 199
Cuts at: 7886 8031
Size: 145
Fragments arranged by size:
4384 1218 859 771 276 199 172 145
7
BspHI T'CATG_A
Cuts at: 0 6047 7596 8031
Size: 6047 1549 435
Fragments arranged by size:
6047 1549 435
BsrDI GCAATG_nn'
Cuts at: 0 1456 1553 1564 4974 8031
Size: 1456 97 11 3410 3057
Fragments arranged by size:
3410 3057 1456 97 11
BsrGI T'GTAC_A
Cuts at: 0 1590 3367 3685 4192 5743 8031
Size: 1590 1777 318 507 1551 2288
Fragments arranged by size:
2288 1777 1590 1551 507 318
BssSI C'ACGA_G
Cuts at: 0 6010 7238 8031
Size: 6010 1228 793
Fragments arranged by size:
6010 1228 793
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1575 8031
Size: 1575 6456
BstBI TT'CG_AA
Cuts at: 0 7017 8031
Size: 7017 1014
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 7696 8031
Size: 495 7201 335
Fragments arranged by size:
7201 495 335
BstYI r'GATC_y
Cuts at: 0 464 849 901 927 980 1257 1818 2150
Size: 464 385 52 26 53 277 561 332
Cuts at: 2150 2742 3513 3573 5493 6618 7668 8031
Size: 592 771 60 1920 1125 1050 363
Fragments arranged by size:
1920 1125 1050 771 592 561 464 385
363 332 277 60 53 52 26
BstZ17I GTA'TAC
Cuts at: 0 1068 8031
Size: 1068 6963
BtgI C'CryG_G
Cuts at: 0 755 3027 3430 6501 8031
Size: 755 2272 403 3071 1530
Fragments arranged by size:
3071 2272 1530 755 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2936 6250 6574 8031
Size: 2936 3314 324 1457
Fragments arranged by size:
3314 2936 1457 324
BtsI GCAGTG_nn'
Cuts at: 0 1490 4470 7382 8031
Size: 1490 2980 2912 649
Fragments arranged by size:
2980 2912 1490 649
ClaI AT'CG_AT
Cuts at: 0 805 8031
Size: 805 7226
DraI TTT'AAA
Cuts at: 0 591 2300 2739 3594 5612 8031
Size: 591 1709 439 855 2018 2419
Fragments arranged by size:
2419 2018 1709 855 591 439
DraIII CAC_nnn'GTG
Cuts at: 0 4643 8031
Size: 4643 3388
EaeI y'GGCC_r
Cuts at: 0 859 5603 6874 8031
Size: 859 4744 1271 1157
Fragments arranged by size:
4744 1271 1157 859
EarI CTCTTCn'nnn_
Cuts at: 0 344 2160 2395 5453 6707 6802 7261 7621
Size: 344 1816 235 3058 1254 95 459 360
Cuts at: 7621 7871 8031
Size: 250 160
Fragments arranged by size:
3058 1816 1254 459 360 344 250 235
160 95
EcoICRI GAG'CTC
Cuts at: 0 6295 7884 8031
Size: 6295 1589 147
Fragments arranged by size:
6295 1589 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1334 2123 2699 3281 3404 3530 4027
Size: 1162 172 789 576 582 123 126 497
Cuts at: 4027 4274 4364 4443 4529 4727 4835 5042 6507
Size: 247 90 79 86 198 108 207 1465
Cuts at: 6507 7591 7645 7718 7808 8011 8031
Size: 1084 54 73 90 203 20
Fragments arranged by size:
1465 1162 1084 789 582 576 497 247
207 203 198 172 126 123 108 90
90 86 79 73 54 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 3868 5897 8031
Size: 649 3219 2029 2134
Fragments arranged by size:
3219 2134 2029 649
EcoO109I rG'GnC_Cy
Cuts at: 0 2826 3874 5159 7277 7575 7611 7746 8031
Size: 2826 1048 1285 2118 298 36 135 285
Fragments arranged by size:
2826 2118 1285 1048 298 285 135 36
EcoRI G'AATT_C
Cuts at: 0 4146 4959 6067 8031
Size: 4146 813 1108 1964
Fragments arranged by size:
4146 1964 1108 813
EcoRV GAT'ATC
Cuts at: 0 1553 8031
Size: 1553 6478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 595 1704 4678 8031
Size: 595 1109 2974 3353
Fragments arranged by size:
3353 2974 1109 595
HaeII r_GCGC'y
Cuts at: 0 60 8031
Size: 60 7971
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 876 893 908 925 1504 1536 1605 1637
Size: 876 17 15 17 579 32 69 32
Cuts at: 1637 2710 2742 3117 3149 3189 3221 3364 3396
Size: 1073 32 375 32 40 32 143 32
Cuts at: 3396 3538 3570 3603 3635 5962 5994 6561 6593
Size: 142 32 33 32 2327 32 567 32
Cuts at: 6593 6919 6951 7211 7243 8031
Size: 326 32 260 32 788
Fragments arranged by size:
2327 1073 876 788 579 567 375 326
260 143 142 69 40 33 32 32
32 32 32 32 32 32 32 32
32 32 17 17 15
HincII GTy'rAC
Cuts at: 0 387 919 1727 2240 8031
Size: 387 532 808 513 5791
Fragments arranged by size:
5791 808 532 513 387
HindIII A'AGCT_T
Cuts at: 0 325 1480 1916 3673 6982 8023 8031
Size: 325 1155 436 1757 3309 1041 8
Fragments arranged by size:
3309 1757 1155 1041 436 325 8
HpaI GTT'AAC
Cuts at: 0 387 8031
Size: 387 7644
MfeI C'AATT_G
Cuts at: 0 429 2646 7193 8031
Size: 429 2217 4547 838
Fragments arranged by size:
4547 2217 838 429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 394 1008 2994 3930 4432 4601 5024
Size: 92 302 614 1986 936 502 169 423
Cuts at: 5024 5674 6661 6885 8031
Size: 650 987 224 1146
Fragments arranged by size:
1986 1146 987 936 650 614 502 423
302 224 169 92
MscI TGG'CCA
Cuts at: 0 861 6876 8031
Size: 861 6015 1155
Fragments arranged by size:
6015 1155 861
MslI CAynn'nnrTG
Cuts at: 0 380 488 683 1224 1800 1813 2672 3740
Size: 380 108 195 541 576 13 859 1068
Cuts at: 3740 5216 5283 6649 8031
Size: 1476 67 1366 1382
Fragments arranged by size:
1476 1382 1366 1068 859 576 541 380
195 108 67 13
MspA1I CmG'CkG
Cuts at: 0 1611 2958 3850 3903 6034 6270 6527 7308
Size: 1611 1347 892 53 2131 236 257 781
Cuts at: 7308 7771 7828 8031
Size: 463 57 203
Fragments arranged by size:
2131 1611 1347 892 781 463 257 236
203 57 53
NcoI C'CATG_G
Cuts at: 0 755 8031
Size: 755 7276
NdeI CA'TA_TG
Cuts at: 0 3888 5835 6790 8031
Size: 3888 1947 955 1241
Fragments arranged by size:
3888 1947 1241 955
NsiI A_TGCA'T
Cuts at: 0 1907 4249 6242 6782 7128 8031
Size: 1907 2342 1993 540 346 903
Fragments arranged by size:
2342 1993 1907 903 540 346
NspI r_CATG'y
Cuts at: 0 11 684 729 1146 1203 1835 2380 3148
Size: 11 673 45 417 57 632 545 768
Cuts at: 3148 4251 8031
Size: 1103 3780
Fragments arranged by size:
3780 1103 768 673 632 545 417 57
45 11
PasI CC'CwG_GG
Cuts at: 0 7458 8031
Size: 7458 573
PciI A'CATG_T
Cuts at: 0 7 680 1142 2376 8031
Size: 7 673 462 1234 5655
Fragments arranged by size:
5655 1234 673 462 7
PfoI T'CCnGG_A
Cuts at: 0 7695 8031
Size: 7695 336
PmlI CAC'GTG
Cuts at: 0 7850 8031
Size: 7850 181
PpuMI rG'GwC_Cy
Cuts at: 0 3874 7277 7575 7611 7746 8031
Size: 3874 3403 298 36 135 285
Fragments arranged by size:
3874 3403 298 285 135 36
PshAI GACnn'nnGTC
Cuts at: 0 2837 8031
Size: 2837 5194
PsiI TTA'TAA
Cuts at: 0 1856 4187 4550 6818 8031
Size: 1856 2331 363 2268 1213
Fragments arranged by size:
2331 2268 1856 1213 363
PspOMI G'GGCC_C
Cuts at: 0 5158 8031
Size: 5158 2873
PspXI vC'TCGA_Gb
Cuts at: 0 7680 8031
Size: 7680 351
PstI C_TGCA'G
Cuts at: 0 6703 7409 8031
Size: 6703 706 622
Fragments arranged by size:
6703 706 622
PvuII CAG'CTG
Cuts at: 0 1611 2958 3850 6034 6270 6527 7308 7828
Size: 1611 1347 892 2184 236 257 781 520
Cuts at: 7828 8031
Size: 203
Fragments arranged by size:
2184 1611 1347 892 781 520 257 236
203
SacI G_AGCT'C
Cuts at: 0 6297 7886 8031
Size: 6297 1589 145
Fragments arranged by size:
6297 1589 145
ScaI AGT'ACT
Cuts at: 0 2406 4809 8031
Size: 2406 2403 3222
Fragments arranged by size:
3222 2406 2403
SfcI C'TryA_G
Cuts at: 0 1988 3338 3800 4331 6699 6754 7362 7405
Size: 1988 1350 462 531 2368 55 608 43
Cuts at: 7405 7775 8031
Size: 370 256
Fragments arranged by size:
2368 1988 1350 608 531 462 370 256
55 43
SmlI C'TyrA_G
Cuts at: 0 742 1261 2906 3088 3121 5187 5588 5790
Size: 742 519 1645 182 33 2066 401 202
Cuts at: 5790 6487 7393 7680 8031
Size: 697 906 287 351
Fragments arranged by size:
2066 1645 906 742 697 519 401 351
287 202 182 33
SpeI A'CTAG_T
Cuts at: 0 1729 4030 8031
Size: 1729 2301 4001
Fragments arranged by size:
4001 2301 1729
SphI G_CATG'C
Cuts at: 0 4251 8031
Size: 4251 3780
SspI AAT'ATT
Cuts at: 0 5041 7658 8031
Size: 5041 2617 373
Fragments arranged by size:
5041 2617 373
StuI AGG'CCT
Cuts at: 0 3525 8031
Size: 3525 4506
StyI C'CwwG_G
Cuts at: 0 755 897 7185 7544 8031
Size: 755 142 6288 359 487
Fragments arranged by size:
6288 755 487 359 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1261 1443 4384 8031
Size: 1261 182 2941 3647
Fragments arranged by size:
3647 2941 1261 182
TatI w'GTAC_w
Cuts at: 0 1173 1307 1590 2122 2176 2404 3367 3679
Size: 1173 134 283 532 54 228 963 312
Cuts at: 3679 3685 4108 4192 4649 4807 5743 6770 7247
Size: 6 423 84 457 158 936 1027 477
Cuts at: 7247 8031
Size: 784
Fragments arranged by size:
1173 1027 963 936 784 532 477 457
423 312 283 228 158 134 84 54
6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7410 7442 8031
Size: 7410 32 589
Fragments arranged by size:
7410 589 32
Tth111I GACn'n_nGTC
Cuts at: 0 1357 6137 8031
Size: 1357 4780 1894
Fragments arranged by size:
4780 1894 1357
XbaI T'CTAG_A
Cuts at: 0 1891 6258 8031
Size: 1891 4367 1773
Fragments arranged by size:
4367 1891 1773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2050 3010 5682 7192 8031
Size: 2050 960 2672 1510 839
Fragments arranged by size:
2672 2050 1510 960 839
XhoI C'TCGA_G
Cuts at: 0 7680 8031
Size: 7680 351
XmnI GAAnn'nnTTC
Cuts at: 0 497 1370 3612 4092 4565 6854 8031
Size: 497 873 2242 480 473 2289 1177
Fragments arranged by size:
2289 2242 1177 873 497 480 473
ZraI GAC'GTC
Cuts at: 0 1889 8031
Size: 1889 6142
Enzymes that do cut:
AatII AccI AcuI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PciI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuII SacI ScaI
SfcI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AgeI AhdI AscI AsiSI AvrII
BbeI BbvCI BglI BlpI BmtI BsiEI BsiWI BspEI
BspMI BsrBI BsrFI BssHII BstEII Bsu36I CspCI DrdI
EagI EciI FseI FspI FspAI KasI KpnI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PflMI
PmeI PpiI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI SwaI XmaI