Restriction map for
pF1KA0612
(Linear) (Six-base) MAPSORT of: KIAA0612.seq Check: 7733 from: 1 to: 5391
KIAA0612.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1002 2592 2958 5391
Size: 1002 1590 366 2433
Fragments arranged by size:
2433 1590 1002 366
AccI GT'mk_AC
Cuts at: 0 1294 5391
Size: 1294 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 349 505 1153 1271 3113 4819 5391
Size: 251 98 156 648 118 1842 1706 572
Fragments arranged by size:
1842 1706 648 572 251 156 118 98
AfeI AGC'GCT
Cuts at: 0 406 5391
Size: 406 4985
AflII C'TTAA_G
Cuts at: 0 418 5391
Size: 418 4973
AhdI GACnn_n'nnGTC
Cuts at: 0 86 3492 4734 5391
Size: 86 3406 1242 657
Fragments arranged by size:
3406 1242 657 86
AleI CACnn'nnGTG
Cuts at: 0 2524 5391
Size: 2524 2867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 425 2732 5391
Size: 425 2307 2659
Fragments arranged by size:
2659 2307 425
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2033 2065 5391
Size: 2033 32 3326
Fragments arranged by size:
3326 2033 32
AlwNI CAG_nnn'CTG
Cuts at: 0 438 837 1050 1098 1170 1287 2038 2322
Size: 438 399 213 48 72 117 751 284
Cuts at: 2322 2403 2964 3145 3496 3768 4376 4414 4797
Size: 81 561 181 351 272 608 38 383
Cuts at: 4797 4923 5391
Size: 126 468
Fragments arranged by size:
751 608 561 468 438 399 383 351
284 272 213 181 126 117 81 72
48 38
ApaI G_GGCC'C
Cuts at: 0 387 540 712 753 1411 2381 2681 2792
Size: 387 153 172 41 658 970 300 111
Cuts at: 2792 3450 4130 4153 4192 4234 4603 5305 5391
Size: 658 680 23 39 42 369 702 86
Fragments arranged by size:
970 702 680 658 658 387 369 300
172 153 111 86 42 41 39 23
AvaI C'yCGr_G
Cuts at: 0 705 745 753 1316 1404 2456 3074 3881
Size: 705 40 8 563 88 1052 618 807
Cuts at: 3881 4547 5391
Size: 666 844
Fragments arranged by size:
1052 844 807 705 666 618 563 88
40 8
BamHI G'GATC_C
Cuts at: 0 1781 5391
Size: 1781 3610
BanI G'GyrC_C
Cuts at: 0 735 2334 2452 2469 2526 3304 3456 5391
Size: 735 1599 118 17 57 778 152 1935
Fragments arranged by size:
1935 1599 778 735 152 118 57 17
BanII G_rGCy'C
Cuts at: 0 205 387 540 712 753 851 1331 1385
Size: 205 182 153 172 41 98 480 54
Cuts at: 1385 1411 1540 1925 2147 2158 2381 2681 2792
Size: 26 129 385 222 11 223 300 111
Cuts at: 2792 3450 4051 4130 4153 4192 4234 4603 4666
Size: 658 601 79 23 39 42 369 63
Cuts at: 4666 4969 5305 5391
Size: 303 336 86
Fragments arranged by size:
658 601 480 385 369 336 303 300
223 222 205 182 172 153 129 111
98 86 79 63 54 42 41 39
26 23 11
BbeI G_GCGC'C
Cuts at: 0 739 2338 3460 5391
Size: 739 1599 1122 1931
Fragments arranged by size:
1931 1599 1122 739
BbsI GAAGACnn'nnnn_
Cuts at: 0 1556 1782 3951 4007 5391
Size: 1556 226 2169 56 1384
Fragments arranged by size:
2169 1556 1384 226 56
BbvCI CC'TCA_GC
Cuts at: 0 379 439 1036 3502 4886 5391
Size: 379 60 597 2466 1384 505
Fragments arranged by size:
2466 1384 597 505 379 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2167 2201 2956 2990 3065 3099 3382 3416
Size: 2167 34 755 34 75 34 283 34
Cuts at: 3416 5391
Size: 1975
Fragments arranged by size:
2167 1975 755 283 75 34 34 34
34
BclI T'GATC_A
Cuts at: 0 2807 5391
Size: 2807 2584
BglI GCCn_nnn'nGGC
Cuts at: 0 69 505 699 1618 1940 3418 3454 4229
Size: 69 436 194 919 322 1478 36 775
Cuts at: 4229 4431 4859 5391
Size: 202 428 532
Fragments arranged by size:
1478 919 775 532 436 428 322 202
194 69 36
BglII A'GATC_T
Cuts at: 0 3752 5391
Size: 3752 1639
BlpI GC'TnA_GC
Cuts at: 0 850 1198 2660 5391
Size: 850 348 1462 2731
Fragments arranged by size:
2731 1462 850 348
Bme1580I G_kGCm'C
Cuts at: 0 60 387 540 712 753 1411 2381 2457
Size: 60 327 153 172 41 658 970 76
Cuts at: 2457 2474 2531 2581 2681 2792 3307 3450 4130
Size: 17 57 50 100 111 515 143 680
Cuts at: 4130 4153 4192 4234 4408 4603 5305 5391
Size: 23 39 42 174 195 702 86
Fragments arranged by size:
970 702 680 658 515 327 195 174
172 153 143 111 100 86 76 60
57 50 42 41 39 23 17
BmgBI CAC'GTC
Cuts at: 0 5175 5391
Size: 5175 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 573 1601 2588 2613 2818 4156 5018 5391
Size: 573 1028 987 25 205 1338 862 373
Fragments arranged by size:
1338 1028 987 862 573 373 205 25
BmtI G_CTAG'C
Cuts at: 0 4214 5391
Size: 4214 1177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 993 1089 1647 2931 4560 5391
Size: 993 96 558 1284 1629 831
Fragments arranged by size:
1629 1284 993 831 558 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 68 189 391 547 703 901 1132
Size: 59 9 121 202 156 156 198 231
Cuts at: 1132 1141 1192 1309 1369 1741 1988 2374 2725
Size: 9 51 117 60 372 247 386 351
Cuts at: 2725 3151 3167 3185 3245 3408 3766 3790 3963
Size: 426 16 18 60 163 358 24 173
Cuts at: 3963 4007 4067 4285 4406 4781 4912 5317 5391
Size: 44 60 218 121 375 131 405 74
Fragments arranged by size:
426 405 386 375 372 358 351 247
231 218 202 198 173 163 156 156
131 121 121 117 74 60 60 60
59 51 44 24 18 16 9 9
Bpu10I CC'TnA_GC
Cuts at: 0 379 439 1036 2031 2264 3502 4886 4968
Size: 379 60 597 995 233 1238 1384 82
Cuts at: 4968 5084 5391
Size: 116 307
Fragments arranged by size:
1384 1238 995 597 379 307 233 116
82 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2203 3176 4981 5391
Size: 2203 973 1805 410
Fragments arranged by size:
2203 1805 973 410
BsaI GGTCTCn'nnnn_
Cuts at: 0 3143 3479 3878 4552 5391
Size: 3143 336 399 674 839
Fragments arranged by size:
3143 839 674 399 336
BsaAI yAC'GTr
Cuts at: 0 591 5391
Size: 591 4800
BsaBI GATnn'nnATC
Cuts at: 0 2806 5391
Size: 2806 2585
BsaHI Gr'CG_yC
Cuts at: 0 736 2335 3457 5391
Size: 736 1599 1122 1934
Fragments arranged by size:
1934 1599 1122 736
BsaWI w'CCGG_w
Cuts at: 0 1226 2163 4561 5391
Size: 1226 937 2398 830
Fragments arranged by size:
2398 1226 937 830
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 1367 1397 1720 1750 3379 3409
Size: 187 30 1150 30 323 30 1629 30
Cuts at: 3409 3844 3874 4998 5028 5391
Size: 435 30 1124 30 363
Fragments arranged by size:
1629 1150 1124 435 363 323 187 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 187 544 931 985 1234 1629 1729
Size: 125 62 357 387 54 249 395 100
Cuts at: 1729 1787 2137 2140 3152 3295 3328 3497 3631
Size: 58 350 3 1012 143 33 169 134
Cuts at: 3631 3634 3637 3640 3643 3652 3763 3835 3838
Size: 3 3 3 3 9 111 72 3
Cuts at: 3838 3853 3862 3865 3868 4050 4055 4674 5006
Size: 15 9 3 3 182 5 619 332
Cuts at: 5006 5385 5388 5391
Size: 379 3 3
Fragments arranged by size:
1012 619 395 387 379 357 350 332
249 182 169 143 134 125 111 100
72 62 58 54 33 15 9 9
5 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 253 623 1154 1490 1746 2473 2693 2742
Size: 253 370 531 336 256 727 220 49
Cuts at: 2742 2951 3154 3286 3300 3449 3642 3713 3912
Size: 209 203 132 14 149 193 71 199
Cuts at: 3912 3918 4227 4476 4909 5063 5391
Size: 6 309 249 433 154 328
Fragments arranged by size:
727 531 433 370 336 328 309 256
253 249 220 209 203 199 193 154
149 132 71 49 14 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 850 1300 1384 1536 2794 5391
Size: 131 719 450 84 152 1258 2597
Fragments arranged by size:
2597 1258 719 450 152 131 84
BsiEI CG_ry'CG
Cuts at: 0 606 3576 5391
Size: 606 2970 1815
Fragments arranged by size:
2970 1815 606
BsiHKAI G_wGCw'C
Cuts at: 0 205 851 1331 1385 1925 2147 3210 3444
Size: 205 646 480 54 540 222 1063 234
Cuts at: 3444 4249 5391
Size: 805 1142
Fragments arranged by size:
1142 1063 805 646 540 480 234 222
205 54
BsmI GAATG_Cn'
Cuts at: 0 960 1032 5391
Size: 960 72 4359
Fragments arranged by size:
4359 960 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 188 670 5391
Size: 188 482 4721
Fragments arranged by size:
4721 482 188
Bsp1286I G_dGCh'C
Cuts at: 0 60 205 387 540 712 753 851 1331
Size: 60 145 182 153 172 41 98 480
Cuts at: 1331 1385 1411 1540 1925 2147 2158 2381 2457
Size: 54 26 129 385 222 11 223 76
Cuts at: 2457 2474 2531 2581 2681 2792 3210 3307 3444
Size: 17 57 50 100 111 418 97 137
Cuts at: 3444 3450 4051 4130 4153 4192 4234 4249 4408
Size: 6 601 79 23 39 42 15 159
Cuts at: 4408 4603 4666 4969 5305 5391
Size: 195 63 303 336 86
Fragments arranged by size:
601 480 418 385 336 303 223 222
195 182 172 159 153 145 137 129
111 100 98 97 86 79 76 63
60 57 54 50 42 41 39 26
23 17 15 11 6
BspEI T'CCGG_A
Cuts at: 0 1226 5391
Size: 1226 4165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1002 2375 2592 2958 3663 3912 5391
Size: 1002 1373 217 366 705 249 1479
Fragments arranged by size:
1479 1373 1002 705 366 249 217
BsrBI CCG'CTC
Cuts at: 0 687 4503 5033 5391
Size: 687 3816 530 358
Fragments arranged by size:
3816 687 530 358
BsrDI GCAATG_nn'
Cuts at: 0 512 3907 5391
Size: 512 3395 1484
Fragments arranged by size:
3395 1484 512
BsrFI r'CCGG_y
Cuts at: 0 4223 5391
Size: 4223 1168
BssSI C'ACGA_G
Cuts at: 0 3250 3562 5391
Size: 3250 312 1829
Fragments arranged by size:
3250 1829 312
BstAPI GCAn_nnn'nTGC
Cuts at: 0 828 2964 3214 5391
Size: 828 2136 250 2177
Fragments arranged by size:
2177 2136 828 250
BstXI CCAn_nnnn'nTGG
Cuts at: 0 213 525 4138 4302 5295 5391
Size: 213 312 3613 164 993 96
Fragments arranged by size:
3613 993 312 213 164 96
BstYI r'GATC_y
Cuts at: 0 1416 1781 2801 3752 3764 4086 4703 4793
Size: 1416 365 1020 951 12 322 617 90
Cuts at: 4793 5391
Size: 598
Fragments arranged by size:
1416 1020 951 617 598 365 322 90
12
Bsu36I CC'TnA_GG
Cuts at: 0 562 1688 2120 4627 5312 5391
Size: 562 1126 432 2507 685 79
Fragments arranged by size:
2507 1126 685 562 432 79
BtgI C'CryG_G
Cuts at: 0 22 44 52 212 2921 3006 3570 4251
Size: 22 22 8 160 2709 85 564 681
Cuts at: 4251 5123 5391
Size: 872 268
Fragments arranged by size:
2709 872 681 564 268 160 85 22
22 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 108 924 3758 5391
Size: 108 816 2834 1633
Fragments arranged by size:
2834 1633 816 108
BtsI GCAGTG_nn'
Cuts at: 0 58 114 2100 2428 2937 3302 4098 4521
Size: 58 56 1986 328 509 365 796 423
Cuts at: 4521 4637 5391
Size: 116 754
Fragments arranged by size:
1986 796 754 509 423 365 328 116
58 56
ClaI AT'CG_AT
Cuts at: 0 2834 5391
Size: 2834 2557
DraIII CAC_nnn'GTG
Cuts at: 0 2238 5391
Size: 2238 3153
EaeI y'GGCC_r
Cuts at: 0 1932 2012 3059 3377 3573 3951 4254 4277
Size: 1932 80 1047 318 196 378 303 23
Cuts at: 4277 5391
Size: 1114
Fragments arranged by size:
1932 1114 1047 378 318 303 196 80
23
EagI C'GGCC_G
Cuts at: 0 3573 5391
Size: 3573 1818
EarI CTCTTCn'nnn_
Cuts at: 0 439 461 481 2567 3602 3608 3629 3824
Size: 439 22 20 2086 1035 6 21 195
Cuts at: 3824 3830 4603 4763 5373 5391
Size: 6 773 160 610 18
Fragments arranged by size:
2086 1035 773 610 439 195 160 22
21 20 18 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1384 3034 4608 5391
Size: 1384 1650 1574 783
Fragments arranged by size:
1650 1574 1384 783
EcoICRI GAG'CTC
Cuts at: 0 203 849 1329 1383 1923 2145 5391
Size: 203 646 480 54 540 222 3246
Fragments arranged by size:
3246 646 540 480 222 203 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 68 189 251 349 391 505 547
Size: 59 9 121 62 98 42 114 42
Cuts at: 547 703 901 1132 1141 1153 1192 1271 1309
Size: 156 198 231 9 12 39 79 38
Cuts at: 1309 1369 1741 1988 2374 2725 3113 3151 3167
Size: 60 372 247 386 351 388 38 16
Cuts at: 3167 3185 3245 3408 3766 3790 3963 4007 4067
Size: 18 60 163 358 24 173 44 60
Cuts at: 4067 4285 4406 4781 4819 4912 5317 5391
Size: 218 121 375 38 93 405 74
Fragments arranged by size:
405 388 386 375 372 358 351 247
231 218 198 173 163 156 121 121
114 98 93 79 74 62 60 60
60 59 44 42 42 39 38 38
38 24 18 16 12 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 294 421 883 967 1375 5315 5391
Size: 294 127 462 84 408 3940 76
Fragments arranged by size:
3940 462 408 294 127 84 76
EcoO109I rG'GnC_Cy
Cuts at: 0 33 383 384 536 574 709 749 750
Size: 33 350 1 152 38 135 40 1
Cuts at: 750 1095 1408 1596 1825 1941 2035 2169 2319
Size: 345 313 188 229 116 94 134 150
Cuts at: 2319 2326 2378 2400 2677 2678 2789 3142 3245
Size: 7 52 22 277 1 111 353 103
Cuts at: 3245 3255 3351 4127 4150 4188 4189 4231 4432
Size: 10 96 776 23 38 1 42 201
Cuts at: 4432 4453 4599 4600 5056 5077 5274 5301 5302
Size: 21 146 1 456 21 197 27 1
Cuts at: 5302 5391
Size: 89
Fragments arranged by size:
776 456 353 350 345 313 277 229
201 197 188 152 150 146 135 134
116 111 103 96 94 89 52 42
40 38 38 33 27 23 22 21
21 10 7 1 1 1 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 468 1867 3822 4789 5391
Size: 468 1399 1955 967 602
Fragments arranged by size:
1955 1399 967 602 468
FspI TGC'GCA
Cuts at: 0 664 2992 5391
Size: 664 2328 2399
Fragments arranged by size:
2399 2328 664
FspAI rTGC'GCAy
Cuts at: 0 2992 5391
Size: 2992 2399
HaeII r_GCGC'y
Cuts at: 0 408 525 739 1551 2338 3121 3460 4264
Size: 408 117 214 812 787 783 339 804
Cuts at: 4264 5391
Size: 1127
Fragments arranged by size:
1127 812 804 787 783 408 339 214
117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 187 219 1720 1752 1766 1798
Size: 72 32 83 32 1501 32 14 32
Cuts at: 1798 1957 1989 3142 3174 3478 3510 3844 3876
Size: 159 32 1153 32 304 32 334 32
Cuts at: 3876 4720 4752 5391
Size: 844 32 639
Fragments arranged by size:
1501 1153 844 639 334 304 159 83
72 32 32 32 32 32 32 32
32 32 14
HincII GTy'rAC
Cuts at: 0 2498 5391
Size: 2498 2893
KasI G'GCGC_C
Cuts at: 0 735 2334 3456 5391
Size: 735 1599 1122 1935
Fragments arranged by size:
1935 1599 1122 735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1658 1955 2876 5030 5312 5391
Size: 1658 297 921 2154 282 79
Fragments arranged by size:
2154 1658 921 297 282 79
MscI TGG'CCA
Cuts at: 0 3379 3953 4279 5391
Size: 3379 574 326 1112
Fragments arranged by size:
3379 1112 574 326
MslI CAynn'nnrTG
Cuts at: 0 2294 2524 2908 5215 5391
Size: 2294 230 384 2307 176
Fragments arranged by size:
2307 2294 384 230 176
MspA1I CmG'CkG
Cuts at: 0 627 672 837 933 1158 1170 1284 1750
Size: 627 45 165 96 225 12 114 466
Cuts at: 1750 1857 1864 2226 2481 2712 2955 2961 4283
Size: 107 7 362 255 231 243 6 1322
Cuts at: 4283 4352 4932 5391
Size: 69 580 459
Fragments arranged by size:
1322 627 580 466 459 362 255 243
231 225 165 114 107 96 69 45
12 7 6
NaeI GCC'GGC
Cuts at: 0 4225 5391
Size: 4225 1166
NarI GG'CG_CC
Cuts at: 0 736 2335 3457 5391
Size: 736 1599 1122 1934
Fragments arranged by size:
1934 1599 1122 736
NcoI C'CATG_G
Cuts at: 0 44 52 5123 5391
Size: 44 8 5071 268
Fragments arranged by size:
5071 268 44 8
NgoMIV G'CCGG_C
Cuts at: 0 4223 5391
Size: 4223 1168
NheI G'CTAG_C
Cuts at: 0 4210 5391
Size: 4210 1181
NspI r_CATG'y
Cuts at: 0 1143 4008 5391
Size: 1143 2865 1383
Fragments arranged by size:
2865 1383 1143
PasI CC'CwG_GG
Cuts at: 0 387 1543 2322 2681 2797 3139 3339 3435
Size: 387 1156 779 359 116 342 200 96
Cuts at: 3435 3490 3509 4427 5305 5391
Size: 55 19 918 878 86
Fragments arranged by size:
1156 918 878 779 387 359 342 200
116 96 86 55 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 28 1050 2238 3145 3496 5124 5391
Size: 28 1022 1188 907 351 1628 267
Fragments arranged by size:
1628 1188 1022 907 351 267 28
PfoI T'CCnGG_A
Cuts at: 0 3201 3599 3649 3767 3815 4237 5294 5391
Size: 3201 398 50 118 48 422 1057 97
Fragments arranged by size:
3201 1057 422 398 118 97 50 48
PmlI CAC'GTG
Cuts at: 0 591 5391
Size: 591 4800
PpuMI rG'GwC_Cy
Cuts at: 0 33 574 1596 1825 3245 3255 3351 5391
Size: 33 541 1022 229 1420 10 96 2040
Fragments arranged by size:
2040 1420 1022 541 229 96 33 10
PshAI GACnn'nnGTC
Cuts at: 0 3932 5391
Size: 3932 1459
PspOMI G'GGCC_C
Cuts at: 0 383 536 708 749 1407 2377 2677 2788
Size: 383 153 172 41 658 970 300 111
Cuts at: 2788 3446 4126 4149 4188 4230 4599 5301 5391
Size: 658 680 23 39 42 369 702 90
Fragments arranged by size:
970 702 680 658 658 383 369 300
172 153 111 90 42 41 39 23
PstI C_TGCA'G
Cuts at: 0 152 551 647 932 1103 1238 2729 2960
Size: 152 399 96 285 171 135 1491 231
Cuts at: 2960 2969 3405 4105 4644 5391
Size: 9 436 700 539 747
Fragments arranged by size:
1491 747 700 539 436 399 285 231
171 152 135 96 9
PvuI CG_AT'CG
Cuts at: 0 606 5391
Size: 606 4785
PvuII CAG'CTG
Cuts at: 0 837 933 1170 1284 1750 1864 2226 2481
Size: 837 96 237 114 466 114 362 255
Cuts at: 2481 2955 2961 4283 5391
Size: 474 6 1322 1108
Fragments arranged by size:
1322 1108 837 474 466 362 255 237
114 114 96 6
SacI G_AGCT'C
Cuts at: 0 205 851 1331 1385 1925 2147 5391
Size: 205 646 480 54 540 222 3244
Fragments arranged by size:
3244 646 540 480 222 205 54
SanDI GG'GwC_CC
Cuts at: 0 33 574 1596 1825 3245 5391
Size: 33 541 1022 229 1420 2146
Fragments arranged by size:
2146 1420 1022 541 229 33
SapI GCTCTTCn'nnn_
Cuts at: 0 439 4763 5391
Size: 439 4324 628
Fragments arranged by size:
4324 628 439
SfcI C'TryA_G
Cuts at: 0 148 547 643 928 1099 1234 2725 2956
Size: 148 399 96 285 171 135 1491 231
Cuts at: 2956 2965 3401 3792 4101 4415 4640 5391
Size: 9 436 391 309 314 225 751
Fragments arranged by size:
1491 751 436 399 391 314 309 285
231 225 171 148 135 96 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1940 3418 4229 4431 5391
Size: 1940 1478 811 202 960
Fragments arranged by size:
1940 1478 960 811 202
SfoI GGC'GCC
Cuts at: 0 737 2336 3458 5391
Size: 737 1599 1122 1933
Fragments arranged by size:
1933 1599 1122 737
SmaI CCC'GGG
Cuts at: 0 707 747 755 1318 1406 2458 4549 5391
Size: 707 40 8 563 88 1052 2091 842
Fragments arranged by size:
2091 1052 842 707 563 88 40 8
SmlI C'TyrA_G
Cuts at: 0 418 2218 3191 4960 5391
Size: 418 1800 973 1769 431
Fragments arranged by size:
1800 1769 973 431 418
SrfI GCCC'GGGC
Cuts at: 0 2458 5391
Size: 2458 2933
StuI AGG'CCT
Cuts at: 0 3412 4135 4399 5391
Size: 3412 723 264 992
Fragments arranged by size:
3412 992 723 264
StyI C'CwwG_G
Cuts at: 0 44 52 1298 1412 1449 2342 2673 3183
Size: 44 8 1246 114 37 893 331 510
Cuts at: 3183 3477 4532 5123 5391
Size: 294 1055 591 268
Fragments arranged by size:
1246 1055 893 591 510 331 294 268
114 44 37 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2355 2440 2514 2934 3113 3331 3448 5391
Size: 2355 85 74 420 179 218 117 1943
Fragments arranged by size:
2355 1943 420 218 179 117 85 74
TatI w'GTAC_w
Cuts at: 0 1871 2934 4988 5391
Size: 1871 1063 2054 403
Fragments arranged by size:
2054 1871 1063 403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1367 1399 1636 1668 3379 3411 5391
Size: 1367 32 237 32 1711 32 1980
Fragments arranged by size:
1980 1711 1367 237 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1732 5391
Size: 1732 3659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2349 2626 3283 4843 5172 5391
Size: 2349 277 657 1560 329 219
Fragments arranged by size:
2349 1560 657 329 277 219
XmaI C'CCGG_G
Cuts at: 0 705 745 753 1316 1404 2456 4547 5391
Size: 705 40 8 563 88 1052 2091 844
Fragments arranged by size:
2091 1052 844 705 563 88 40 8
XmnI GAAnn'nnTTC
Cuts at: 0 4772 5391
Size: 4772 619
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AhdI AleI AlfI
AloI AlwNI ApaI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuI PvuII SacI
SanDI SapI SfcI SfiI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BciVI BfrBI BsiWI BspHI
BsrGI BssHII BstBI BstEII BstZ17I CspCI DraI DrdI
EcoRI EcoRV FseI HindIII HpaI KpnI MfeI MluI
NdeI NotI NruI NsiI PacI PciI PmeI PpiI
PsiI PspXI PsrI RsrII SacII SalI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
XbaI XhoI ZraI