Restriction map for
pF1KA0611
(Linear) (Six-base) MAPSORT of: KIAA0611.seq Check: 6882 from: 1 to: 2733
KIAA0611.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
AatII G_ACGT'C
Cuts at: 0 1961 2733
Size: 1961 772
AccI GT'mk_AC
Cuts at: 0 368 1073 2733
Size: 368 705 1660
Fragments arranged by size:
1660 705 368
Acc65I G'GTAC_C
Cuts at: 0 575 2733
Size: 575 2158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1891 2733
Size: 538 1353 842
Fragments arranged by size:
1353 842 538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 973 1270 1781 2733
Size: 973 297 511 952
Fragments arranged by size:
973 952 511 297
AlwNI CAG_nnn'CTG
Cuts at: 0 1191 2733
Size: 1191 1542
ApaI G_GGCC'C
Cuts at: 0 916 2733
Size: 916 1817
ApaLI G'TGCA_C
Cuts at: 0 254 958 1465 1647 2443 2733
Size: 254 704 507 182 796 290
Fragments arranged by size:
796 704 507 290 254 182
AvaI C'yCGr_G
Cuts at: 0 91 697 1367 1732 1779 2733
Size: 91 606 670 365 47 954
Fragments arranged by size:
954 670 606 365 91 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 383 416 2733
Size: 383 33 2317
Fragments arranged by size:
2317 383 33
BanI G'GyrC_C
Cuts at: 0 86 517 575 2733
Size: 86 431 58 2158
Fragments arranged by size:
2158 431 86 58
BanII G_rGCy'C
Cuts at: 0 916 2318 2733
Size: 916 1402 415
Fragments arranged by size:
1402 916 415
BbsI GAAGACnn'nnnn_
Cuts at: 0 1064 1148 2700 2733
Size: 1064 84 1552 33
Fragments arranged by size:
1552 1064 84 33
BbvCI CC'TCA_GC
Cuts at: 0 1037 1168 2116 2733
Size: 1037 131 948 617
Fragments arranged by size:
1037 948 617 131
BciVI GTATCCnnnnn_n'
Cuts at: 0 122 2318 2733
Size: 122 2196 415
Fragments arranged by size:
2196 415 122
BclI T'GATC_A
Cuts at: 0 1029 2733
Size: 1029 1704
BglI GCCn_nnn'nGGC
Cuts at: 0 973 1538 2733
Size: 973 565 1195
Fragments arranged by size:
1195 973 565
BglII A'GATC_T
Cuts at: 0 1211 2733
Size: 1211 1522
BlpI GC'TnA_GC
Cuts at: 0 2548 2733
Size: 2548 185
Bme1580I G_kGCm'C
Cuts at: 0 89 258 522 916 962 1469 1651 1770
Size: 89 169 264 394 46 507 182 119
Cuts at: 1770 1849 1875 2447 2733
Size: 79 26 572 286
Fragments arranged by size:
572 507 394 286 264 182 169 119
89 79 46 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 279 2733
Size: 279 2454
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 131 2733
Size: 131 2602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1292 1528 1600 1654 1813 2733
Size: 699 593 236 72 54 159 920
Fragments arranged by size:
920 699 593 236 159 72 54
Bpu10I CC'TnA_GC
Cuts at: 0 728 1037 1168 2116 2312 2733
Size: 728 309 131 948 196 421
Fragments arranged by size:
948 728 421 309 196 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1789 2317 2665 2733
Size: 1789 528 348 68
Fragments arranged by size:
1789 528 348 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 95 537 1142 1576 2733
Size: 95 442 605 434 1157
Fragments arranged by size:
1157 605 442 434 95
BsaAI yAC'GTr
Cuts at: 0 339 1646 2733
Size: 339 1307 1087
Fragments arranged by size:
1307 1087 339
BsaBI GATnn'nnATC
Cuts at: 0 2383 2733
Size: 2383 350
BsaHI Gr'CG_yC
Cuts at: 0 1958 2733
Size: 1958 775
BsaWI w'CCGG_w
Cuts at: 0 431 2733
Size: 431 2302
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1045 1075 1289 1319 1336 1366 1459 1489
Size: 1045 30 214 30 17 30 93 30
Cuts at: 1489 2130 2160 2176 2206 2554 2584 2733
Size: 641 30 16 30 348 30 149
Fragments arranged by size:
1045 641 348 214 149 93 30 30
30 30 30 30 30 17 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 337 589 1429 2733
Size: 337 252 840 1304
Fragments arranged by size:
1304 840 337 252
BseYI C'CCAG_C
Cuts at: 0 81 668 735 881 2710 2733
Size: 81 587 67 146 1829 23
Fragments arranged by size:
1829 587 146 81 67 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1955 2733
Size: 1345 610 778
Fragments arranged by size:
1345 778 610
BsiEI CG_ry'CG
Cuts at: 0 1473 1853 2344 2733
Size: 1473 380 491 389
Fragments arranged by size:
1473 491 389 380
BsiHKAI G_wGCw'C
Cuts at: 0 258 962 1388 1469 1651 2318 2401 2447
Size: 258 704 426 81 182 667 83 46
Cuts at: 2447 2733
Size: 286
Fragments arranged by size:
704 667 426 286 258 182 83 81
46
BsmI GAATG_Cn'
Cuts at: 0 1599 1665 2733
Size: 1599 66 1068
Fragments arranged by size:
1599 1068 66
Bsp1286I G_dGCh'C
Cuts at: 0 89 258 522 916 962 1388 1469 1651
Size: 89 169 264 394 46 426 81 182
Cuts at: 1651 1770 1849 1875 2318 2401 2447 2733
Size: 119 79 26 443 83 46 286
Fragments arranged by size:
443 426 394 286 264 182 169 119
89 83 81 79 46 46 26
BspEI T'CCGG_A
Cuts at: 0 431 2733
Size: 431 2302
BspHI T'CATG_A
Cuts at: 0 440 2528 2733
Size: 440 2088 205
Fragments arranged by size:
2088 440 205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 587 2696 2733
Size: 587 2109 37
Fragments arranged by size:
2109 587 37
BsrBI CCG'CTC
Cuts at: 0 1475 2733
Size: 1475 1258
BsrDI GCAATG_nn'
Cuts at: 0 1959 2733
Size: 1959 774
BsrFI r'CCGG_y
Cuts at: 0 374 900 2066 2733
Size: 374 526 1166 667
Fragments arranged by size:
1166 667 526 374
BsrGI T'GTAC_A
Cuts at: 0 662 2733
Size: 662 2071
BssSI C'ACGA_G
Cuts at: 0 386 1387 2587 2733
Size: 386 1001 1200 146
Fragments arranged by size:
1200 1001 386 146
BstBI TT'CG_AA
Cuts at: 0 678 2733
Size: 678 2055
BstEII G'GTnAC_C
Cuts at: 0 1671 1707 2733
Size: 1671 36 1026
Fragments arranged by size:
1671 1026 36
BstYI r'GATC_y
Cuts at: 0 326 506 1187 1211 2325 2733
Size: 326 180 681 24 1114 408
Fragments arranged by size:
1114 681 408 326 180 24
BstZ17I GTA'TAC
Cuts at: 0 1074 2733
Size: 1074 1659
Bsu36I CC'TnA_GG
Cuts at: 0 1588 2733
Size: 1588 1145
BtgI C'CryG_G
Cuts at: 0 704 1490 1716 2733
Size: 704 786 226 1017
Fragments arranged by size:
1017 786 704 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 963 2550 2595 2733
Size: 963 1587 45 138
Fragments arranged by size:
1587 963 138 45
ClaI AT'CG_AT
Cuts at: 0 2597 2733
Size: 2597 136
DraIII CAC_nnn'GTG
Cuts at: 0 996 2451 2733
Size: 996 1455 282
Fragments arranged by size:
1455 996 282
DrdI GACnn_nn'nnGTC
Cuts at: 0 352 2281 2733
Size: 352 1929 452
Fragments arranged by size:
1929 452 352
EaeI y'GGCC_r
Cuts at: 0 293 1487 1850 2064 2085 2341 2453 2733
Size: 293 1194 363 214 21 256 112 280
Fragments arranged by size:
1194 363 293 280 256 214 112 21
EagI C'GGCC_G
Cuts at: 0 1850 2341 2733
Size: 1850 491 392
Fragments arranged by size:
1850 491 392
EarI CTCTTCn'nnn_
Cuts at: 0 91 1343 2733
Size: 91 1252 1390
Fragments arranged by size:
1390 1252 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 954 2554 2733
Size: 954 1600 179
Fragments arranged by size:
1600 954 179
EcoICRI GAG'CTC
Cuts at: 0 2316 2733
Size: 2316 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 699 1292 1528 1600 1654 1813 1891
Size: 538 161 593 236 72 54 159 78
Cuts at: 1891 2733
Size: 842
Fragments arranged by size:
842 593 538 236 161 159 78 72
54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1749 1906 2733
Size: 1749 157 827
Fragments arranged by size:
1749 827 157
EcoO109I rG'GnC_Cy
Cuts at: 0 11 65 894 912 1172 2733
Size: 11 54 829 18 260 1561
Fragments arranged by size:
1561 829 260 54 18 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 528 2379 2733
Size: 528 1851 354
Fragments arranged by size:
1851 528 354
HaeII r_GCGC'y
Cuts at: 0 2419 2503 2733
Size: 2419 84 230
Fragments arranged by size:
2419 230 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 759 791 2204 2236 2272 2304 2733
Size: 759 32 1413 32 36 32 429
Fragments arranged by size:
1413 759 429 36 32 32 32
HincII GTy'rAC
Cuts at: 0 357 2733
Size: 357 2376
KpnI G_GTAC'C
Cuts at: 0 579 2733
Size: 579 2154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1040 1322 2414 2733
Size: 635 405 282 1092 319
Fragments arranged by size:
1092 635 405 319 282
MscI TGG'CCA
Cuts at: 0 1489 2455 2733
Size: 1489 966 278
Fragments arranged by size:
1489 966 278
MslI CAynn'nnrTG
Cuts at: 0 1252 1763 2404 2533 2599 2628 2733
Size: 1252 511 641 129 66 29 105
Fragments arranged by size:
1252 641 511 129 105 66 29
MspA1I CmG'CkG
Cuts at: 0 668 735 1167 1554 1718 2660 2733
Size: 668 67 432 387 164 942 73
Fragments arranged by size:
942 668 432 387 164 73 67
NaeI GCC'GGC
Cuts at: 0 376 2733
Size: 376 2357
NgoMIV G'CCGG_C
Cuts at: 0 374 2733
Size: 374 2359
PasI CC'CwG_GG
Cuts at: 0 279 2733
Size: 279 2454
PfoI T'CCnGG_A
Cuts at: 0 2270 2733
Size: 2270 463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1807 1839 2422 2454 2733
Size: 1807 32 583 32 279
Fragments arranged by size:
1807 583 279 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 894 1172 2733
Size: 894 278 1561
Fragments arranged by size:
1561 894 278
PspOMI G'GGCC_C
Cuts at: 0 912 2733
Size: 912 1821
PspXI vC'TCGA_Gb
Cuts at: 0 1732 2733
Size: 1732 1001
PstI C_TGCA'G
Cuts at: 0 584 1559 2034 2733
Size: 584 975 475 699
Fragments arranged by size:
975 699 584 475
PvuII CAG'CTG
Cuts at: 0 668 735 1167 1554 2660 2733
Size: 668 67 432 387 1106 73
Fragments arranged by size:
1106 668 432 387 73 67
SacI G_AGCT'C
Cuts at: 0 2318 2733
Size: 2318 415
SacII CC_GC'GG
Cuts at: 0 1719 2733
Size: 1719 1014
ScaI AGT'ACT
Cuts at: 0 1810 2733
Size: 1810 923
SfcI C'TryA_G
Cuts at: 0 369 580 1207 1555 2030 2733
Size: 369 211 627 348 475 703
Fragments arranged by size:
703 627 475 369 348 211
SmaI CCC'GGG
Cuts at: 0 699 2733
Size: 699 2034
SmlI C'TyrA_G
Cuts at: 0 1732 1804 2332 2680 2733
Size: 1732 72 528 348 53
Fragments arranged by size:
1732 528 348 72 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 391 660 895 1894 2733
Size: 391 269 235 999 839
Fragments arranged by size:
999 839 391 269 235
TatI w'GTAC_w
Cuts at: 0 140 662 852 1328 1808 2603 2733
Size: 140 522 190 476 480 795 130
Fragments arranged by size:
795 522 480 476 190 140 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 144 176 1107 1139 1289 1321 1336 1368
Size: 144 32 931 32 150 32 15 32
Cuts at: 1368 1459 1491 1513 1545 2235 2267 2733
Size: 91 32 22 32 690 32 466
Fragments arranged by size:
931 690 466 150 144 91 32 32
32 32 32 32 32 22 15
XhoI C'TCGA_G
Cuts at: 0 1732 2733
Size: 1732 1001
XmaI C'CCGG_G
Cuts at: 0 697 2733
Size: 697 2036
XmnI GAAnn'nnTTC
Cuts at: 0 1744 2733
Size: 1744 989
ZraI GAC'GTC
Cuts at: 0 1959 2733
Size: 1959 774
Enzymes that do cut:
AatII AccI Acc65I AcuI AlfI AlwNI ApaI ApaLI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NgoMIV PasI PfoI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SacII ScaI SfcI SmaI
SmlI TaqII TatI TstI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstAPI
BstXI BtsI CspCI DraI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PshAI PsiI PsrI PvuI RsrII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XcmI