Restriction map for
pF1KSDA0607
(Linear) (Six-base) MAPSORT of: KIAA0607.seq Check: 1325 from: 1 to: 2136
KIAA0607.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 133 1080 1260 2136
Size: 133 947 180 876
Fragments arranged by size:
947 876 180 133
AatII G_ACGT'C
Cuts at: 0 1603 2136
Size: 1603 533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1690 2136
Size: 814 876 446
Fragments arranged by size:
876 814 446
AfeI AGC'GCT
Cuts at: 0 61 2136
Size: 61 2075
AflII C'TTAA_G
Cuts at: 0 1015 2136
Size: 1015 1121
AleI CACnn'nnGTG
Cuts at: 0 931 1471 2136
Size: 931 540 665
Fragments arranged by size:
931 665 540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 482 837 2136
Size: 482 355 1299
Fragments arranged by size:
1299 482 355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1843 1875 2136
Size: 1843 32 261
Fragments arranged by size:
1843 261 32
AlwNI CAG_nnn'CTG
Cuts at: 0 423 534 678 1011 1200 1947 2124 2136
Size: 423 111 144 333 189 747 177 12
Fragments arranged by size:
747 423 333 189 177 144 111 12
ApaI G_GGCC'C
Cuts at: 0 1310 1379 1407 2136
Size: 1310 69 28 729
Fragments arranged by size:
1310 729 69 28
AvaI C'yCGr_G
Cuts at: 0 365 1379 1724 1785 1990 2136
Size: 365 1014 345 61 205 146
Fragments arranged by size:
1014 365 345 205 146 61
BamHI G'GATC_C
Cuts at: 0 836 980 1139 2136
Size: 836 144 159 997
Fragments arranged by size:
997 836 159 144
BanI G'GyrC_C
Cuts at: 0 228 458 475 1147 1352 2136
Size: 228 230 17 672 205 784
Fragments arranged by size:
784 672 230 228 205 17
BanII G_rGCy'C
Cuts at: 0 75 596 668 772 1118 1310 1379 1407
Size: 75 521 72 104 346 192 69 28
Cuts at: 1407 1670 1792 1856 2135 2136
Size: 263 122 64 279 1
Fragments arranged by size:
521 346 279 263 192 122 104 75
72 69 64 28 1
BbeI G_GCGC'C
Cuts at: 0 232 2136
Size: 232 1904
BbvCI CC'TCA_GC
Cuts at: 0 644 2136
Size: 644 1492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 122 687 721 2136
Size: 88 34 565 34 1415
Fragments arranged by size:
1415 565 88 34 34
BclI T'GATC_A
Cuts at: 0 1391 1553 2136
Size: 1391 162 583
Fragments arranged by size:
1391 583 162
BglI GCCn_nnn'nGGC
Cuts at: 0 280 742 811 1066 1156 1249 1282 1314
Size: 280 462 69 255 90 93 33 32
Cuts at: 1314 2136
Size: 822
Fragments arranged by size:
822 462 280 255 93 90 69 33
32
BlpI GC'TnA_GC
Cuts at: 0 766 2136
Size: 766 1370
Bme1580I G_kGCm'C
Cuts at: 0 512 710 928 1310 1379 1407 2136
Size: 512 198 218 382 69 28 729
Fragments arranged by size:
729 512 382 218 198 69 28
BmtI G_CTAG'C
Cuts at: 0 1810 2136
Size: 1810 326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1185 1345 2136
Size: 1185 160 791
Fragments arranged by size:
1185 791 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 934 1682 1936 2136
Size: 76 858 748 254 200
Fragments arranged by size:
858 748 254 200 76
Bpu10I CC'TnA_GC
Cuts at: 0 644 1687 2136
Size: 644 1043 449
Fragments arranged by size:
1043 644 449
BsaI GGTCTCn'nnnn_
Cuts at: 0 1159 2136
Size: 1159 977
BsaAI yAC'GTr
Cuts at: 0 1770 2136
Size: 1770 366
BsaBI GATnn'nnATC
Cuts at: 0 1390 2136
Size: 1390 746
BsaHI Gr'CG_yC
Cuts at: 0 229 1565 1600 2136
Size: 229 1336 35 536
Fragments arranged by size:
1336 536 229 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1843 1873 2136
Size: 1843 30 263
Fragments arranged by size:
1843 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 931 1177 1252 1261 1327 1660 2095 2136
Size: 931 246 75 9 66 333 435 41
Fragments arranged by size:
931 435 333 246 75 66 41 9
BseYI C'CCAG_C
Cuts at: 0 1265 2136
Size: 1265 871
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1054 1639 2136
Size: 773 281 585 497
Fragments arranged by size:
773 585 497 281
BsiHKAI G_wGCw'C
Cuts at: 0 668 772 1856 2136
Size: 668 104 1084 280
Fragments arranged by size:
1084 668 280 104
BsmI GAATG_Cn'
Cuts at: 0 721 994 2136
Size: 721 273 1142
Fragments arranged by size:
1142 721 273
BsmBI CGTCTCn'nnnn_
Cuts at: 0 214 913 1316 2077 2136
Size: 214 699 403 761 59
Fragments arranged by size:
761 699 403 214 59
Bsp1286I G_dGCh'C
Cuts at: 0 75 512 596 668 710 772 928 1118
Size: 75 437 84 72 42 62 156 190
Cuts at: 1118 1310 1379 1407 1670 1792 1856 2135 2136
Size: 192 69 28 263 122 64 279 1
Fragments arranged by size:
437 279 263 192 190 156 122 84
75 72 69 64 62 42 28 1
BspHI T'CATG_A
Cuts at: 0 1049 1595 2136
Size: 1049 546 541
Fragments arranged by size:
1049 546 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 133 608 1080 1091 1260 1516 1853 2136
Size: 133 475 472 11 169 256 337 283
Fragments arranged by size:
475 472 337 283 256 169 133 11
BsrBI CCG'CTC
Cuts at: 0 395 2136
Size: 395 1741
BsrDI GCAATG_nn'
Cuts at: 0 464 2136
Size: 464 1672
BsrFI r'CCGG_y
Cuts at: 0 1882 2042 2136
Size: 1882 160 94
Fragments arranged by size:
1882 160 94
BssSI C'ACGA_G
Cuts at: 0 1039 1531 2136
Size: 1039 492 605
Fragments arranged by size:
1039 605 492
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1011 1947 2136
Size: 1011 936 189
Fragments arranged by size:
1011 936 189
BstEII G'GTnAC_C
Cuts at: 0 948 1536 2136
Size: 948 588 600
Fragments arranged by size:
948 600 588
BstYI r'GATC_y
Cuts at: 0 173 726 836 980 1139 1385 2136
Size: 173 553 110 144 159 246 751
Fragments arranged by size:
751 553 246 173 159 144 110
BtsI GCAGTG_nn'
Cuts at: 0 300 1009 1444 1808 1971 2125 2136
Size: 300 709 435 364 163 154 11
Fragments arranged by size:
709 435 364 300 163 154 11
EaeI y'GGCC_r
Cuts at: 0 308 605 1157 1274 1283 1315 1458 1655
Size: 308 297 552 117 9 32 143 197
Cuts at: 1655 1936 2000 2087 2136
Size: 281 64 87 49
Fragments arranged by size:
552 308 297 281 197 143 117 87
64 49 32 9
EarI CTCTTCn'nnn_
Cuts at: 0 1709 1756 2136
Size: 1709 47 380
Fragments arranged by size:
1709 380 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 186 604 2136
Size: 186 418 1532
Fragments arranged by size:
1532 418 186
EcoICRI GAG'CTC
Cuts at: 0 666 770 1854 2136
Size: 666 104 1084 282
Fragments arranged by size:
1084 666 282 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 814 934 1682 1690 1936 2136
Size: 76 738 120 748 8 246 200
Fragments arranged by size:
748 738 246 200 120 76 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 493 625 1087 1234 1393 2136
Size: 79 414 132 462 147 159 743
Fragments arranged by size:
743 462 414 159 147 132 79
EcoO109I rG'GnC_Cy
Cuts at: 0 372 451 531 618 1306 1376 1403 1404
Size: 372 79 80 87 688 70 27 1
Cuts at: 1404 2136
Size: 732
Fragments arranged by size:
732 688 372 87 80 79 70 27
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1134 2136
Size: 1134 1002
HaeII r_GCGC'y
Cuts at: 0 63 232 2136
Size: 63 169 1904
Fragments arranged by size:
1904 169 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 2136
Size: 1156 32 948
Fragments arranged by size:
1156 948 32
KasI G'GCGC_C
Cuts at: 0 228 2136
Size: 228 1908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 2136
Size: 854 1282
MscI TGG'CCA
Cuts at: 0 1276 1285 1317 1460 1657 2136
Size: 1276 9 32 143 197 479
Fragments arranged by size:
1276 479 197 143 32 9
MslI CAynn'nnrTG
Cuts at: 0 515 931 1471 1594 2136
Size: 515 416 540 123 542
Fragments arranged by size:
542 540 515 416 123
MspA1I CmG'CkG
Cuts at: 0 675 1197 1741 1918 1934 1956 2136
Size: 675 522 544 177 16 22 180
Fragments arranged by size:
675 544 522 180 177 22 16
NarI GG'CG_CC
Cuts at: 0 229 2136
Size: 229 1907
NheI G'CTAG_C
Cuts at: 0 1806 2136
Size: 1806 330
NspI r_CATG'y
Cuts at: 0 1872 2136
Size: 1872 264
PasI CC'CwG_GG
Cuts at: 0 1303 1662 2136
Size: 1303 359 474
Fragments arranged by size:
1303 474 359
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 1272 2136
Size: 534 738 864
Fragments arranged by size:
864 738 534
PfoI T'CCnGG_A
Cuts at: 0 853 983 2136
Size: 853 130 1153
Fragments arranged by size:
1153 853 130
PmlI CAC'GTG
Cuts at: 0 1770 2136
Size: 1770 366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1843 1875 2136
Size: 1843 32 261
Fragments arranged by size:
1843 261 32
PpuMI rG'GwC_Cy
Cuts at: 0 372 2136
Size: 372 1764
PspOMI G'GGCC_C
Cuts at: 0 1306 1375 1403 2136
Size: 1306 69 28 733
Fragments arranged by size:
1306 733 69 28
PstI C_TGCA'G
Cuts at: 0 295 734 1091 1436 1505 1850 2072 2136
Size: 295 439 357 345 69 345 222 64
Fragments arranged by size:
439 357 345 345 295 222 69 64
PvuII CAG'CTG
Cuts at: 0 675 1197 1741 1956 2136
Size: 675 522 544 215 180
Fragments arranged by size:
675 544 522 215 180
SacI G_AGCT'C
Cuts at: 0 668 772 1856 2136
Size: 668 104 1084 280
Fragments arranged by size:
1084 668 280 104
SanDI GG'GwC_CC
Cuts at: 0 372 2136
Size: 372 1764
SapI GCTCTTCn'nnn_
Cuts at: 0 1709 2136
Size: 1709 427
SbfI CC_TGCA'GG
Cuts at: 0 1850 2136
Size: 1850 286
SfcI C'TryA_G
Cuts at: 0 291 431 730 1087 1432 1501 1846 2068
Size: 291 140 299 357 345 69 345 222
Cuts at: 2068 2136
Size: 68
Fragments arranged by size:
357 345 345 299 291 222 140 69
68
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 280 1249 1282 1314 2136
Size: 280 969 33 32 822
Fragments arranged by size:
969 822 280 33 32
SfoI GGC'GCC
Cuts at: 0 230 2136
Size: 230 1906
SmaI CCC'GGG
Cuts at: 0 367 1381 1787 2136
Size: 367 1014 406 349
Fragments arranged by size:
1014 406 367 349
SmlI C'TyrA_G
Cuts at: 0 1015 2136
Size: 1015 1121
SpeI A'CTAG_T
Cuts at: 0 1614 2136
Size: 1614 522
SphI G_CATG'C
Cuts at: 0 1872 2136
Size: 1872 264
StuI AGG'CCT
Cuts at: 0 1876 2136
Size: 1876 260
StyI C'CwwG_G
Cuts at: 0 128 221 2111 2136
Size: 128 93 1890 25
Fragments arranged by size:
1890 128 93 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 459 1135 2136
Size: 256 203 676 1001
Fragments arranged by size:
1001 676 256 203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1131 1163 2136
Size: 1131 32 973
Fragments arranged by size:
1131 973 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1310 2136
Size: 1310 826
XmaI C'CCGG_G
Cuts at: 0 365 1379 1785 2136
Size: 365 1014 406 351
Fragments arranged by size:
1014 406 365 351
ZraI GAC'GTC
Cuts at: 0 1601 2136
Size: 1601 535
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AleI AlfI AloI
AlwNI ApaI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BclI BglI BlpI Bme1580I BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstYI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NheI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SbfI SfcI SfiI
SfoI SmaI SmlI SpeI SphI StuI StyI TaqII
TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflIII AgeI AhdI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BciVI BfrBI
BglII BmgBI BmrI BpuEI BsaWI BsiEI BsiWI BspEI
BsrGI BssHII BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI ScaI SexAI SgfI SgrAI SnaBI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI