Restriction map for
pF1KSDA0604
(Linear) (Six-base) MAPSORT of: KIAA0604.seq Check: 6249 from: 1 to: 2295
KIAA0604.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 181 286 479 891 1104 2295
Size: 181 105 193 412 213 1191
Fragments arranged by size:
1191 412 213 193 181 105
AccI GT'mk_AC
Cuts at: 0 704 2295
Size: 704 1591
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1583 2029 2295
Size: 484 1099 446 266
Fragments arranged by size:
1099 484 446 266
AfeI AGC'GCT
Cuts at: 0 526 2295
Size: 526 1769
AflIII A'CryG_T
Cuts at: 0 1583 2295
Size: 1583 712
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1133 1165 2295
Size: 1133 32 1130
Fragments arranged by size:
1133 1130 32
AlwNI CAG_nnn'CTG
Cuts at: 0 21 278 440 582 906 1008 1977 2067
Size: 21 257 162 142 324 102 969 90
Cuts at: 2067 2295
Size: 228
Fragments arranged by size:
969 324 257 228 162 142 102 90
21
ApaI G_GGCC'C
Cuts at: 0 629 2295
Size: 629 1666
ApoI r'AATT_y
Cuts at: 0 1556 1590 2295
Size: 1556 34 705
Fragments arranged by size:
1556 705 34
AvaI C'yCGr_G
Cuts at: 0 82 779 2295
Size: 82 697 1516
Fragments arranged by size:
1516 697 82
AvrII C'CTAG_G
Cuts at: 0 240 2295
Size: 240 2055
BanI G'GyrC_C
Cuts at: 0 193 1011 2295
Size: 193 818 1284
Fragments arranged by size:
1284 818 193
BanII G_rGCy'C
Cuts at: 0 346 576 629 767 1127 1523 2084 2152
Size: 346 230 53 138 360 396 561 68
Cuts at: 2152 2295
Size: 143
Fragments arranged by size:
561 396 360 346 230 143 138 68
53
BbeI G_GCGC'C
Cuts at: 0 1015 2295
Size: 1015 1280
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 523 1453 1707 2295
Size: 80 443 930 254 588
Fragments arranged by size:
930 588 443 254 80
BclI T'GATC_A
Cuts at: 0 1385 2295
Size: 1385 910
BglI GCCn_nnn'nGGC
Cuts at: 0 408 1278 1872 2255 2295
Size: 408 870 594 383 40
Fragments arranged by size:
870 594 408 383 40
BglII A'GATC_T
Cuts at: 0 971 2295
Size: 971 1324
BlpI GC'TnA_GC
Cuts at: 0 509 2295
Size: 509 1786
Bme1580I G_kGCm'C
Cuts at: 0 629 944 2209 2295
Size: 629 315 1265 86
Fragments arranged by size:
1265 629 315 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 859 2295
Size: 859 1436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 340 480 928 1258 1537 2295
Size: 196 144 140 448 330 279 758
Fragments arranged by size:
758 448 330 279 196 144 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1179 2295
Size: 1051 128 1116
Fragments arranged by size:
1116 1051 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 254 581 1234 1643 2295
Size: 79 175 327 653 409 652
Fragments arranged by size:
653 652 409 327 175 79
BsaHI Gr'CG_yC
Cuts at: 0 116 1012 1307 2295
Size: 116 896 295 988
Fragments arranged by size:
988 896 295 116
BsaWI w'CCGG_w
Cuts at: 0 1397 1898 2295
Size: 1397 501 397
Fragments arranged by size:
1397 501 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 1912 1942 2295
Size: 352 30 1530 30 353
Fragments arranged by size:
1530 353 352 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 577 979 1426 2071 2295
Size: 577 402 447 645 224
Fragments arranged by size:
645 577 447 402 224
BseYI C'CCAG_C
Cuts at: 0 21 567 575 861 1642 1977 2202 2295
Size: 21 546 8 286 781 335 225 93
Fragments arranged by size:
781 546 335 286 225 93 21 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1205 2295
Size: 197 1008 1090
Fragments arranged by size:
1090 1008 197
BsiEI CG_ry'CG
Cuts at: 0 1968 2295
Size: 1968 327
BsiHKAI G_wGCw'C
Cuts at: 0 821 1127 2131 2152 2295
Size: 821 306 1004 21 143
Fragments arranged by size:
1004 821 306 143 21
BsmI GAATG_Cn'
Cuts at: 0 296 1686 2295
Size: 296 1390 609
Fragments arranged by size:
1390 609 296
Bsp1286I G_dGCh'C
Cuts at: 0 346 576 629 767 821 944 1127 1523
Size: 346 230 53 138 54 123 183 396
Cuts at: 1523 2084 2131 2152 2209 2295
Size: 561 47 21 57 86
Fragments arranged by size:
561 396 346 230 183 138 123 86
57 54 53 47 21
BspEI T'CCGG_A
Cuts at: 0 1397 1898 2295
Size: 1397 501 397
Fragments arranged by size:
1397 501 397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 181 286 479 891 1104 1294 1868 2295
Size: 181 105 193 412 213 190 574 427
Fragments arranged by size:
574 427 412 213 193 190 181 105
BsrDI GCAATG_nn'
Cuts at: 0 744 1988 2295
Size: 744 1244 307
Fragments arranged by size:
1244 744 307
BsrFI r'CCGG_y
Cuts at: 0 1355 2295
Size: 1355 940
BsrGI T'GTAC_A
Cuts at: 0 1595 2295
Size: 1595 700
BssSI C'ACGA_G
Cuts at: 0 882 2155 2295
Size: 882 1273 140
Fragments arranged by size:
1273 882 140
BstAPI GCAn_nnn'nTGC
Cuts at: 0 903 2067 2295
Size: 903 1164 228
Fragments arranged by size:
1164 903 228
BstEII G'GTnAC_C
Cuts at: 0 2166 2295
Size: 2166 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 1530 1617 1948 2295
Size: 126 1404 87 331 347
Fragments arranged by size:
1404 347 331 126 87
BstYI r'GATC_y
Cuts at: 0 971 2295
Size: 971 1324
BtgI C'CryG_G
Cuts at: 0 119 1019 2075 2295
Size: 119 900 1056 220
Fragments arranged by size:
1056 900 220 119
BtsI GCAGTG_nn'
Cuts at: 0 214 507 821 2295
Size: 214 293 314 1474
Fragments arranged by size:
1474 314 293 214
EaeI y'GGCC_r
Cuts at: 0 926 1965 2295
Size: 926 1039 330
Fragments arranged by size:
1039 926 330
EagI C'GGCC_G
Cuts at: 0 1965 2295
Size: 1965 330
EarI CTCTTCn'nnn_
Cuts at: 0 1339 2104 2295
Size: 1339 765 191
Fragments arranged by size:
1339 765 191
EcoICRI GAG'CTC
Cuts at: 0 1125 2150 2295
Size: 1125 1025 145
Fragments arranged by size:
1125 1025 145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 340 480 484 928 1258 1537 1583
Size: 196 144 140 4 444 330 279 46
Cuts at: 1583 2029 2295
Size: 446 266
Fragments arranged by size:
446 444 330 279 266 196 144 140
46 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 882 2295
Size: 286 596 1413
Fragments arranged by size:
1413 596 286
EcoO109I rG'GnC_Cy
Cuts at: 0 104 625 626 674 1328 1416 1737 1767
Size: 104 521 1 48 654 88 321 30
Cuts at: 1767 2295
Size: 528
Fragments arranged by size:
654 528 521 321 104 88 48 30
1
HaeII r_GCGC'y
Cuts at: 0 528 1015 2295
Size: 528 487 1280
Fragments arranged by size:
1280 528 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 2295
Size: 1024 32 1239
Fragments arranged by size:
1239 1024 32
HincII GTy'rAC
Cuts at: 0 1811 2295
Size: 1811 484
KasI G'GCGC_C
Cuts at: 0 1011 2295
Size: 1011 1284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 755 893 1319 1720 2240 2295
Size: 592 163 138 426 401 520 55
Fragments arranged by size:
592 520 426 401 163 138 55
MscI TGG'CCA
Cuts at: 0 928 2295
Size: 928 1367
MslI CAynn'nnrTG
Cuts at: 0 124 301 937 1789 2295
Size: 124 177 636 852 506
Fragments arranged by size:
852 636 506 177 124
MspA1I CmG'CkG
Cuts at: 0 153 174 924 954 1428 1723 2280 2295
Size: 153 21 750 30 474 295 557 15
Fragments arranged by size:
750 557 474 295 153 30 21 15
NarI GG'CG_CC
Cuts at: 0 1012 2295
Size: 1012 1283
NcoI C'CATG_G
Cuts at: 0 119 1019 2295
Size: 119 900 1276
Fragments arranged by size:
1276 900 119
NspI r_CATG'y
Cuts at: 0 1587 1819 2295
Size: 1587 232 476
Fragments arranged by size:
1587 476 232
PasI CC'CwG_GG
Cuts at: 0 629 1419 2272 2295
Size: 629 790 853 23
Fragments arranged by size:
853 790 629 23
PciI A'CATG_T
Cuts at: 0 1583 2295
Size: 1583 712
PflMI CCAn_nnn'nTGG
Cuts at: 0 1025 1977 2295
Size: 1025 952 318
Fragments arranged by size:
1025 952 318
PfoI T'CCnGG_A
Cuts at: 0 317 1514 2295
Size: 317 1197 781
Fragments arranged by size:
1197 781 317
PpuMI rG'GwC_Cy
Cuts at: 0 674 1328 2295
Size: 674 654 967
Fragments arranged by size:
967 674 654
PspOMI G'GGCC_C
Cuts at: 0 625 2295
Size: 625 1670
PstI C_TGCA'G
Cuts at: 0 17 1004 1736 2295
Size: 17 987 732 559
Fragments arranged by size:
987 732 559 17
PvuII CAG'CTG
Cuts at: 0 153 174 924 1428 2280 2295
Size: 153 21 750 504 852 15
Fragments arranged by size:
852 750 504 153 21 15
RsrII CG'GwC_CG
Cuts at: 0 1400 2133 2295
Size: 1400 733 162
Fragments arranged by size:
1400 733 162
SacI G_AGCT'C
Cuts at: 0 1127 2152 2295
Size: 1127 1025 143
Fragments arranged by size:
1127 1025 143
SanDI GG'GwC_CC
Cuts at: 0 1328 2295
Size: 1328 967
SapI GCTCTTCn'nnn_
Cuts at: 0 2104 2295
Size: 2104 191
SbfI CC_TGCA'GG
Cuts at: 0 1736 2295
Size: 1736 559
SexAI A'CCwGG_T
Cuts at: 0 1175 1940 2295
Size: 1175 765 355
Fragments arranged by size:
1175 765 355
SfcI C'TryA_G
Cuts at: 0 13 544 678 1000 1732 2295
Size: 13 531 134 322 732 563
Fragments arranged by size:
732 563 531 322 134 13
SfoI GGC'GCC
Cuts at: 0 1013 2295
Size: 1013 1282
SmlI C'TyrA_G
Cuts at: 0 1030 1194 2295
Size: 1030 164 1101
Fragments arranged by size:
1101 1030 164
SphI G_CATG'C
Cuts at: 0 1819 2295
Size: 1819 476
StuI AGG'CCT
Cuts at: 0 292 2295
Size: 292 2003
StyI C'CwwG_G
Cuts at: 0 119 240 1019 1311 1463 1610 1763 1830
Size: 119 121 779 292 152 147 153 67
Cuts at: 1830 2295
Size: 465
Fragments arranged by size:
779 465 292 153 152 147 121 119
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 347 853 1220 2121 2185 2295
Size: 347 506 367 901 64 110
Fragments arranged by size:
901 506 367 347 110 64
TatI w'GTAC_w
Cuts at: 0 47 1595 1928 2295
Size: 47 1548 333 367
Fragments arranged by size:
1548 367 333 47
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 370 2295
Size: 370 1925
XmnI GAAnn'nnTTC
Cuts at: 0 427 2295
Size: 427 1868
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AloI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbeI BbsI BclI
BglI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII
RsrII SacI SanDI SapI SbfI SexAI SfcI SfoI
SmlI SphI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
ApaLI AscI AseI AsiSI BaeI BamHI BbvCI BcgI
BciVI BfrBI BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsiWI BsmBI BspHI BsrBI BssHII BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI SacII SalI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI