Restriction map for
pF1KA0600
(Linear) (Six-base) MAPSORT of: KIAA0600.seq Check: 4668 from: 1 to: 3111
KIAA0600.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1908 2965 3111
Size: 1908 1057 146
Fragments arranged by size:
1908 1057 146
AccI GT'mk_AC
Cuts at: 0 1180 3111
Size: 1180 1931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2486 2550 3111
Size: 2486 64 561
Fragments arranged by size:
2486 561 64
AflIII A'CryG_T
Cuts at: 0 1091 1425 3111
Size: 1091 334 1686
Fragments arranged by size:
1686 1091 334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2178 3111
Size: 2178 933
AlwNI CAG_nnn'CTG
Cuts at: 0 225 264 279 420 477 489 775 1218
Size: 225 39 15 141 57 12 286 443
Cuts at: 1218 1521 1530 1560 1905 1965 2455 3111
Size: 303 9 30 345 60 490 656
Fragments arranged by size:
656 490 443 345 303 286 225 141
60 57 39 30 15 12 9
ApaI G_GGCC'C
Cuts at: 0 734 1850 3111
Size: 734 1116 1261
Fragments arranged by size:
1261 1116 734
ApoI r'AATT_y
Cuts at: 0 565 3111
Size: 565 2546
AvaI C'yCGr_G
Cuts at: 0 1715 3056 3111
Size: 1715 1341 55
Fragments arranged by size:
1715 1341 55
AvrII C'CTAG_G
Cuts at: 0 1119 3111
Size: 1119 1992
BanI G'GyrC_C
Cuts at: 0 904 1929 2371 3111
Size: 904 1025 442 740
Fragments arranged by size:
1025 904 740 442
BanII G_rGCy'C
Cuts at: 0 186 196 239 593 648 734 1053 1312
Size: 186 10 43 354 55 86 319 259
Cuts at: 1312 1704 1726 1850 2014 2204 2463 3089 3111
Size: 392 22 124 164 190 259 626 22
Fragments arranged by size:
626 392 354 319 259 259 190 186
164 124 86 55 43 22 22 10
BbeI G_GCGC'C
Cuts at: 0 1933 3111
Size: 1933 1178
BbsI GAAGACnn'nnnn_
Cuts at: 0 1757 1781 3111
Size: 1757 24 1330
Fragments arranged by size:
1757 1330 24
BbvCI CC'TCA_GC
Cuts at: 0 553 1040 1838 1936 3111
Size: 553 487 798 98 1175
Fragments arranged by size:
1175 798 553 487 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1816 1850 3111
Size: 1816 34 1261
Fragments arranged by size:
1816 1261 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1867 3111
Size: 1867 1244
BglI GCCn_nnn'nGGC
Cuts at: 0 1283 2791 3037 3111
Size: 1283 1508 246 74
Fragments arranged by size:
1508 1283 246 74
BlpI GC'TnA_GC
Cuts at: 0 821 3111
Size: 821 2290
Bme1580I G_kGCm'C
Cuts at: 0 734 1850 1955 3111
Size: 734 1116 105 1156
Fragments arranged by size:
1156 1116 734 105
BmgBI CAC'GTC
Cuts at: 0 1094 2547 3111
Size: 1094 1453 564
Fragments arranged by size:
1453 1094 564
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 1971 2358 3111
Size: 122 1849 387 753
Fragments arranged by size:
1849 753 387 122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 692 1502 2148 3111
Size: 692 810 646 963
Fragments arranged by size:
963 810 692 646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 484 499 544 1195 1315 1372 1582
Size: 442 42 15 45 651 120 57 210
Cuts at: 1582 1777 2121 2191 2770 2937 3111
Size: 195 344 70 579 167 174
Fragments arranged by size:
651 579 442 344 210 195 174 167
120 70 57 45 42 15
Bpu10I CC'TnA_GC
Cuts at: 0 553 1040 1477 1838 1936 3111
Size: 553 487 437 361 98 1175
Fragments arranged by size:
1175 553 487 437 361 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 334 2188 3111
Size: 232 102 1854 923
Fragments arranged by size:
1854 923 232 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 2974 3111
Size: 2974 137
BsaHI Gr'CG_yC
Cuts at: 0 700 919 1930 3111
Size: 700 219 1011 1181
Fragments arranged by size:
1181 1011 700 219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 819 849 3111
Size: 819 30 2262
Fragments arranged by size:
2262 819 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1663 1672 1675 1774 1777 1801 1804 1807
Size: 1663 9 3 99 3 24 3 3
Cuts at: 1807 1834 1855 1873 2124 2648 3001 3097 3111
Size: 27 21 18 251 524 353 96 14
Fragments arranged by size:
1663 524 353 251 99 96 27 24
21 18 14 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 160 638 955 1281 1590 1689 1898 2375
Size: 160 478 317 326 309 99 209 477
Cuts at: 2375 3111
Size: 736
Fragments arranged by size:
736 478 477 326 317 309 209 160
99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 2947 3111
Size: 58 2889 164
Fragments arranged by size:
2889 164 58
BsiHKAI G_wGCw'C
Cuts at: 0 239 1258 1375 1744 2204 2408 3111
Size: 239 1019 117 369 460 204 703
Fragments arranged by size:
1019 703 460 369 239 204 117
BsmBI CGTCTCn'nnnn_
Cuts at: 0 740 1100 2537 3111
Size: 740 360 1437 574
Fragments arranged by size:
1437 740 574 360
Bsp1286I G_dGCh'C
Cuts at: 0 186 196 239 593 648 734 1053 1258
Size: 186 10 43 354 55 86 319 205
Cuts at: 1258 1312 1375 1704 1726 1744 1850 1955 2014
Size: 54 63 329 22 18 106 105 59
Cuts at: 2014 2204 2408 2463 3089 3111
Size: 190 204 55 626 22
Fragments arranged by size:
626 354 329 319 205 204 190 186
106 105 86 63 59 55 55 54
43 22 22 18 10
BspHI T'CATG_A
Cuts at: 0 1250 3111
Size: 1250 1861
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1908 2965 3111
Size: 1908 1057 146
Fragments arranged by size:
1908 1057 146
BsrBI CCG'CTC
Cuts at: 0 686 825 3111
Size: 686 139 2286
Fragments arranged by size:
2286 686 139
BsrDI GCAATG_nn'
Cuts at: 0 530 1379 2373 2585 3111
Size: 530 849 994 212 526
Fragments arranged by size:
994 849 530 526 212
BsrFI r'CCGG_y
Cuts at: 0 105 1240 2951 3075 3111
Size: 105 1135 1711 124 36
Fragments arranged by size:
1711 1135 124 105 36
BssSI C'ACGA_G
Cuts at: 0 2593 3111
Size: 2593 518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 225 264 366 393 489 1341 1914 3111
Size: 225 39 102 27 96 852 573 1197
Fragments arranged by size:
1197 852 573 225 102 96 39 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 135 168 2262 2368 3111
Size: 135 33 2094 106 743
Fragments arranged by size:
2094 743 135 106 33
BstYI r'GATC_y
Cuts at: 0 1598 2900 3111
Size: 1598 1302 211
Fragments arranged by size:
1598 1302 211
Bsu36I CC'TnA_GG
Cuts at: 0 1646 3111
Size: 1646 1465
BtgI C'CryG_G
Cuts at: 0 61 134 1133 1314 1395 1462 2261 2361
Size: 61 73 999 181 81 67 799 100
Cuts at: 2361 3008 3089 3111
Size: 647 81 22
Fragments arranged by size:
999 799 647 181 100 81 81 73
67 61 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 969 2407 3111
Size: 969 1438 704
Fragments arranged by size:
1438 969 704
BtsI GCAGTG_nn'
Cuts at: 0 208 1160 2162 2879 3111
Size: 208 952 1002 717 232
Fragments arranged by size:
1002 952 717 232 208
DraIII CAC_nnn'GTG
Cuts at: 0 2117 3111
Size: 2117 994
DrdI GACnn_nn'nnGTC
Cuts at: 0 2026 3111
Size: 2026 1085
EaeI y'GGCC_r
Cuts at: 0 1430 1943 2694 2876 3111
Size: 1430 513 751 182 235
Fragments arranged by size:
1430 751 513 235 182
EarI CTCTTCn'nnn_
Cuts at: 0 452 508 877 2002 2044 2462 3111
Size: 452 56 369 1125 42 418 649
Fragments arranged by size:
1125 649 452 418 369 56 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2613 3111
Size: 2613 498
EcoICRI GAG'CTC
Cuts at: 0 237 2202 3111
Size: 237 1965 909
Fragments arranged by size:
1965 909 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 484 499 544 1195 1315 1372 1582
Size: 442 42 15 45 651 120 57 210
Cuts at: 1582 1777 2121 2191 2486 2550 2770 2937 3111
Size: 195 344 70 295 64 220 167 174
Fragments arranged by size:
651 442 344 295 220 210 195 174
167 120 70 64 57 45 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1222 1759 2962 3111
Size: 1222 537 1203 149
Fragments arranged by size:
1222 1203 537 149
EcoO109I rG'GnC_Cy
Cuts at: 0 731 1206 1518 1846 1962 2050 2334 3111
Size: 731 475 312 328 116 88 284 777
Fragments arranged by size:
777 731 475 328 312 284 116 88
EcoRI G'AATT_C
Cuts at: 0 565 3111
Size: 565 2546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2055 3111
Size: 2055 1056
FspI TGC'GCA
Cuts at: 0 2149 3111
Size: 2149 962
FspAI rTGC'GCAy
Cuts at: 0 2149 3111
Size: 2149 962
HaeII r_GCGC'y
Cuts at: 0 247 1933 3008 3111
Size: 247 1686 1075 103
Fragments arranged by size:
1686 1075 247 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 18 45 50 819 851 1023 1055
Size: 13 5 27 5 769 32 172 32
Cuts at: 1055 2204 2236 3111
Size: 1149 32 875
Fragments arranged by size:
1149 875 769 172 32 32 32 27
13 5 5
HincII GTy'rAC
Cuts at: 0 1181 3111
Size: 1181 1930
KasI G'GCGC_C
Cuts at: 0 1929 3111
Size: 1929 1182
MscI TGG'CCA
Cuts at: 0 1432 1945 2696 2878 3111
Size: 1432 513 751 182 233
Fragments arranged by size:
1432 751 513 233 182
MslI CAynn'nnrTG
Cuts at: 0 85 1030 1319 1384 1438 2098 2155 2260
Size: 85 945 289 65 54 660 57 105
Cuts at: 2260 2366 2426 3111
Size: 106 60 685
Fragments arranged by size:
945 685 660 289 106 105 85 65
60 57 54
MspA1I CmG'CkG
Cuts at: 0 63 264 336 432 438 450 474 489
Size: 63 201 72 96 6 12 24 15
Cuts at: 489 1464 1500 1530 1590 1905 2712 2941 3111
Size: 975 36 30 60 315 807 229 170
Fragments arranged by size:
975 807 315 229 201 170 96 72
63 60 36 30 24 15 12 6
NaeI GCC'GGC
Cuts at: 0 3077 3111
Size: 3077 34
NarI GG'CG_CC
Cuts at: 0 1930 3111
Size: 1930 1181
NcoI C'CATG_G
Cuts at: 0 134 2261 2361 3008 3089 3111
Size: 134 2127 100 647 81 22
Fragments arranged by size:
2127 647 134 100 81 22
NgoMIV G'CCGG_C
Cuts at: 0 3075 3111
Size: 3075 36
NspI r_CATG'y
Cuts at: 0 1324 1342 1443 3111
Size: 1324 18 101 1668
Fragments arranged by size:
1668 1324 101 18
PasI CC'CwG_GG
Cuts at: 0 694 1965 1996 3111
Size: 694 1271 31 1115
Fragments arranged by size:
1271 1115 694 31
PflMI CCAn_nnn'nTGG
Cuts at: 0 638 1965 2455 3111
Size: 638 1327 490 656
Fragments arranged by size:
1327 656 638 490
PfoI T'CCnGG_A
Cuts at: 0 560 1559 3111
Size: 560 999 1552
Fragments arranged by size:
1552 999 560
PpuMI rG'GwC_Cy
Cuts at: 0 2334 3111
Size: 2334 777
PshAI GACnn'nnGTC
Cuts at: 0 2652 3111
Size: 2652 459
PspOMI G'GGCC_C
Cuts at: 0 730 1846 3111
Size: 730 1116 1265
Fragments arranged by size:
1265 1116 730
PstI C_TGCA'G
Cuts at: 0 230 269 341 1130 1337 1526 1910 2196
Size: 230 39 72 789 207 189 384 286
Cuts at: 2196 2828 3111
Size: 632 283
Fragments arranged by size:
789 632 384 286 283 230 207 189
72 39
PvuII CAG'CTG
Cuts at: 0 264 336 489 1530 1590 2712 3111
Size: 264 72 153 1041 60 1122 399
Fragments arranged by size:
1122 1041 399 264 153 72 60
SacI G_AGCT'C
Cuts at: 0 239 2204 3111
Size: 239 1965 907
Fragments arranged by size:
1965 907 239
SacII CC_GC'GG
Cuts at: 0 64 1465 3111
Size: 64 1401 1646
Fragments arranged by size:
1646 1401 64
SalI G'TCGA_C
Cuts at: 0 1179 3111
Size: 1179 1932
SapI GCTCTTCn'nnn_
Cuts at: 0 452 508 2002 3111
Size: 452 56 1494 1109
Fragments arranged by size:
1494 1109 452 56
SfcI C'TryA_G
Cuts at: 0 226 265 337 1126 1333 1522 1585 1906
Size: 226 39 72 789 207 189 63 321
Cuts at: 1906 2192 2285 2308 2824 3111
Size: 286 93 23 516 287
Fragments arranged by size:
789 516 321 287 286 226 207 189
93 72 63 39 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3037 3111
Size: 3037 74
SfoI GGC'GCC
Cuts at: 0 1931 3111
Size: 1931 1180
SmaI CCC'GGG
Cuts at: 0 1717 3058 3111
Size: 1717 1341 53
Fragments arranged by size:
1717 1341 53
SmlI C'TyrA_G
Cuts at: 0 247 349 2203 3111
Size: 247 102 1854 908
Fragments arranged by size:
1854 908 247 102
SpeI A'CTAG_T
Cuts at: 0 1410 3111
Size: 1410 1701
SphI G_CATG'C
Cuts at: 0 1324 1443 3111
Size: 1324 119 1668
Fragments arranged by size:
1668 1324 119
StyI C'CwwG_G
Cuts at: 0 47 134 1119 2261 2361 2831 3008 3089
Size: 47 87 985 1142 100 470 177 81
Cuts at: 3089 3111
Size: 22
Fragments arranged by size:
1142 985 470 177 100 87 81 47
22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1654 2389 2653 2998 3111
Size: 1654 735 264 345 113
Fragments arranged by size:
1654 735 345 264 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1387 1419 2474 2506 2508 2540 3111
Size: 1387 32 1055 32 2 32 571
Fragments arranged by size:
1387 1055 571 32 32 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2544 3111
Size: 2544 567
XmaI C'CCGG_G
Cuts at: 0 1715 3056 3111
Size: 1715 1341 55
Fragments arranged by size:
1715 1341 55
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SalI SapI SfcI SfiI SfoI
SmaI SmlI SpeI SphI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI BaeI BamHI BclI
BfrBI BglII BmtI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BspEI BsrGI BssHII BstBI BstEII BstZ17I ClaI
CspCI DraI EagI EcoRV FseI HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SanDI SbfI ScaI SexAI SgfI SgrAI
SnaBI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI