Restriction map for
pF1KA0597
(Linear) (Six-base) MAPSORT of: KIAA0597.seq Check: 5641 from: 1 to: 2730
KIAA0597.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1579 1666 2730
Size: 827 752 87 1064
Fragments arranged by size:
1064 827 752 87
AflIII A'CryG_T
Cuts at: 0 864 969 2730
Size: 864 105 1761
Fragments arranged by size:
1761 864 105
AhdI GACnn_n'nnGTC
Cuts at: 0 1345 2730
Size: 1345 1385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 191 2730
Size: 191 2539
AlwNI CAG_nnn'CTG
Cuts at: 0 1644 2730
Size: 1644 1086
ApaLI G'TGCA_C
Cuts at: 0 453 2730
Size: 453 2277
ApoI r'AATT_y
Cuts at: 0 411 1072 1365 1881 2730
Size: 411 661 293 516 849
Fragments arranged by size:
849 661 516 411 293
AvaI C'yCGr_G
Cuts at: 0 1579 2730
Size: 1579 1151
BanII G_rGCy'C
Cuts at: 0 373 762 2024 2210 2730
Size: 373 389 1262 186 520
Fragments arranged by size:
1262 520 389 373 186
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 861 1287 1905 2161 2730
Size: 23 838 426 618 256 569
Fragments arranged by size:
838 618 569 426 256 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 1675 2730
Size: 1675 1055
BclI T'GATC_A
Cuts at: 0 615 2147 2730
Size: 615 1532 583
Fragments arranged by size:
1532 615 583
BglI GCCn_nnn'nGGC
Cuts at: 0 213 2730
Size: 213 2517
BglII A'GATC_T
Cuts at: 0 1081 2730
Size: 1081 1649
Bme1580I G_kGCm'C
Cuts at: 0 457 2292 2730
Size: 457 1835 438
Fragments arranged by size:
1835 457 438
BmgBI CAC'GTC
Cuts at: 0 452 972 2730
Size: 452 520 1758
Fragments arranged by size:
1758 520 452
BmrI ACTGGGnnnn_n'
Cuts at: 0 1105 2295 2730
Size: 1105 1190 435
Fragments arranged by size:
1190 1105 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 563 1232 1544 1819 2204 2730
Size: 182 381 669 312 275 385 526
Fragments arranged by size:
669 526 385 381 312 275 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 1128 1709 2047 2144 2730
Size: 1128 581 338 97 586
Fragments arranged by size:
1128 586 581 338 97
BsaAI yAC'GTr
Cuts at: 0 1498 2730
Size: 1498 1232
BsaBI GATnn'nnATC
Cuts at: 0 1385 2730
Size: 1385 1345
BsaWI w'CCGG_w
Cuts at: 0 2390 2730
Size: 2390 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1291 1321 1835 1865 2730
Size: 1291 30 514 30 865
Fragments arranged by size:
1291 865 514 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 709 712 724 2252 2730
Size: 706 3 3 12 1528 478
Fragments arranged by size:
1528 706 478 12 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 2730
Size: 617 2113
BsiHKAI G_wGCw'C
Cuts at: 0 373 457 516 2024 2730
Size: 373 84 59 1508 706
Fragments arranged by size:
1508 706 373 84 59
BsmI GAATG_Cn'
Cuts at: 0 118 466 786 833 2730
Size: 118 348 320 47 1897
Fragments arranged by size:
1897 348 320 118 47
Bsp1286I G_dGCh'C
Cuts at: 0 373 457 516 762 2024 2210 2292 2730
Size: 373 84 59 246 1262 186 82 438
Fragments arranged by size:
1262 438 373 246 186 84 82 59
BspEI T'CCGG_A
Cuts at: 0 2390 2730
Size: 2390 340
BspHI T'CATG_A
Cuts at: 0 2144 2150 2730
Size: 2144 6 580
Fragments arranged by size:
2144 580 6
BsrDI GCAATG_nn'
Cuts at: 0 2315 2730
Size: 2315 415
BssSI C'ACGA_G
Cuts at: 0 1632 2662 2730
Size: 1632 1030 68
Fragments arranged by size:
1632 1030 68
BstYI r'GATC_y
Cuts at: 0 1081 1692 2730
Size: 1081 611 1038
Fragments arranged by size:
1081 1038 611
Bsu36I CC'TnA_GG
Cuts at: 0 2099 2239 2730
Size: 2099 140 491
Fragments arranged by size:
2099 491 140
BtgI C'CryG_G
Cuts at: 0 8 504 2275 2730
Size: 8 496 1771 455
Fragments arranged by size:
1771 496 455 8
BtsI GCAGTG_nn'
Cuts at: 0 260 653 1645 2730
Size: 260 393 992 1085
Fragments arranged by size:
1085 992 393 260
DraI TTT'AAA
Cuts at: 0 887 1880 2730
Size: 887 993 850
Fragments arranged by size:
993 887 850
DraIII CAC_nnn'GTG
Cuts at: 0 461 463 2730
Size: 461 2 2267
Fragments arranged by size:
2267 461 2
EaeI y'GGCC_r
Cuts at: 0 1974 2730
Size: 1974 756
EarI CTCTTCn'nnn_
Cuts at: 0 683 803 2730
Size: 683 120 1927
Fragments arranged by size:
1927 683 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2556 2590 2730
Size: 2556 34 140
Fragments arranged by size:
2556 140 34
EcoICRI GAG'CTC
Cuts at: 0 371 2022 2730
Size: 371 1651 708
Fragments arranged by size:
1651 708 371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 563 827 1232 1544 1579 1666 1819
Size: 182 381 264 405 312 35 87 153
Cuts at: 1819 2204 2730
Size: 385 526
Fragments arranged by size:
526 405 385 381 312 264 182 153
87 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2501 2730
Size: 2501 229
EcoO109I rG'GnC_Cy
Cuts at: 0 1846 2338 2730
Size: 1846 492 392
Fragments arranged by size:
1846 492 392
EcoRV GAT'ATC
Cuts at: 0 1185 2730
Size: 1185 1545
HaeII r_GCGC'y
Cuts at: 0 2616 2730
Size: 2616 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 1032 1064 1331 1363 2325 2357
Size: 385 32 615 32 267 32 962 32
Cuts at: 2357 2730
Size: 373
Fragments arranged by size:
962 615 385 373 267 32 32 32
32
HincII GTy'rAC
Cuts at: 0 404 1755 2656 2730
Size: 404 1351 901 74
Fragments arranged by size:
1351 901 404 74
HpaI GTT'AAC
Cuts at: 0 1755 2730
Size: 1755 975
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 2730
Size: 506 2224
MslI CAynn'nnrTG
Cuts at: 0 74 569 2149 2730
Size: 74 495 1580 581
Fragments arranged by size:
1580 581 495 74
MspA1I CmG'CkG
Cuts at: 0 10 637 2032 2730
Size: 10 627 1395 698
Fragments arranged by size:
1395 698 627 10
NcoI C'CATG_G
Cuts at: 0 504 2275 2730
Size: 504 1771 455
Fragments arranged by size:
1771 504 455
NdeI CA'TA_TG
Cuts at: 0 22 2730
Size: 22 2708
NspI r_CATG'y
Cuts at: 0 335 535 868 1771 2308 2730
Size: 335 200 333 903 537 422
Fragments arranged by size:
903 537 422 335 333 200
PciI A'CATG_T
Cuts at: 0 864 2730
Size: 864 1866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1562 1594 2730
Size: 1562 32 1136
Fragments arranged by size:
1562 1136 32
PpuMI rG'GwC_Cy
Cuts at: 0 2338 2730
Size: 2338 392
PspXI vC'TCGA_Gb
Cuts at: 0 1579 2730
Size: 1579 1151
PvuII CAG'CTG
Cuts at: 0 637 2032 2730
Size: 637 1395 698
Fragments arranged by size:
1395 698 637
SacI G_AGCT'C
Cuts at: 0 373 2024 2730
Size: 373 1651 706
Fragments arranged by size:
1651 706 373
SacII CC_GC'GG
Cuts at: 0 11 2730
Size: 11 2719
SapI GCTCTTCn'nnn_
Cuts at: 0 803 2730
Size: 803 1927
SexAI A'CCwGG_T
Cuts at: 0 1341 2730
Size: 1341 1389
SfcI C'TryA_G
Cuts at: 0 1548 2730
Size: 1548 1182
SmlI C'TyrA_G
Cuts at: 0 1579 2730
Size: 1579 1151
SnaBI TAC'GTA
Cuts at: 0 1498 2730
Size: 1498 1232
SpeI A'CTAG_T
Cuts at: 0 849 2730
Size: 849 1881
SphI G_CATG'C
Cuts at: 0 335 535 1771 2308 2730
Size: 335 200 1236 537 422
Fragments arranged by size:
1236 537 422 335 200
StuI AGG'CCT
Cuts at: 0 995 1808 2730
Size: 995 813 922
Fragments arranged by size:
995 922 813
StyI C'CwwG_G
Cuts at: 0 504 1193 2249 2275 2730
Size: 504 689 1056 26 455
Fragments arranged by size:
1056 689 504 455 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 26 812 864 2730
Size: 26 786 52 1866
Fragments arranged by size:
1866 786 52 26
TatI w'GTAC_w
Cuts at: 0 82 1392 2730
Size: 82 1310 1338
Fragments arranged by size:
1338 1310 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2096 2128 2282 2314 2730
Size: 2096 32 154 32 416
Fragments arranged by size:
2096 416 154 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2282 2730
Size: 2282 448
XhoI C'TCGA_G
Cuts at: 0 1579 2730
Size: 1579 1151
XmnI GAAnn'nnTTC
Cuts at: 0 1808 2730
Size: 1808 922
Enzymes that do cut:
AcuI AflIII AhdI AlfI AlwNI ApaLI ApoI AvaI
BanII BbsI BciVI BclI BglI BglII Bme1580I BmgBI
BmrI BpmI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BssSI
BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HincII HpaI MmeI MslI MspA1I NcoI NdeI NspI
PciI PpiI PpuMI PspXI PvuII SacI SacII SapI
SexAI SfcI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BfrBI BlpI BmtI
BplI Bpu10I BpuEI BsaHI BseYI BsiEI BsiWI BsmBI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DrdI EagI EcoRI
FalI FseI FspI FspAI HindIII KasI KpnI MfeI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PsrI PstI PvuI RsrII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SrfI
SspI SwaI Tth111I XbaI XmaI ZraI